scholarly journals The Profile of Amino Acids in Sea Horse ((Hippocampus sp)

2021 ◽  
Vol 869 (1) ◽  
pp. 012027
Author(s):  
A A Muhammadar ◽  
Z Nur ◽  
A W Perdana ◽  
M Nasir ◽  
D F Putra ◽  
...  
Keyword(s):  
Analytical Chemistry ◽  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Proximate Analysis ◽  
Ash Content ◽  
Nutritional Values ◽  
Large Size ◽  
Aceh Province ◽  
Gas Chromatography Mass Spectroscopy

Abstract Seahorse (Hippocampus sp.) is a marine fisheries commodity that has high economic and nutritional values. The aims of study were to determine the proximate composition and the profile of the amino acids content in seahorses. The method used refers to the AOAC (Association of Official Analytical Chemistry) for proximate analysis and a GC-MS (Gas Chromatography-Mass Spectroscopy) for the amino acid analysis. This research was done in March 2021. The sea horses were carried out from Simeulue waters, Simeulue district, Aceh Province, Indonesia. Samples were divided into 2 sizes, small size (3.00-5.00 cm) and large size ((5.01-10.00 cm). The analysis was done at laboratorium of Syiah Kuala University. The results of the study was found that the proximate content of the seahorse was water content (6.29%), ash content (25.43%), fat content (0.57%), protein content (39.32%) and carbohydrates ( 28.48%). The amino acid composition of large seahorses and small seahorses consists of four types of essential amino acids, namely leucine, isoleucine, histidine and valine, and six types of non-essential amino acids, namely alanine, glycine, aspartic acid, asparagine, arginine and serine.

scholarly journals AMINO ACID PROFILE AND BIOACTIVE COMPOUNDS OF SEAHORSE Hippocampus comes

2018 ◽  
Vol 9 (2) ◽  
pp. 605-617
Author(s):  
Evi Maya Sari ◽  
Mala Nurilmala ◽  
Asadatun Abdullah
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Bioactive Compounds ◽  
Proximate Composition ◽  
Ethanol Extract ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Ash Content ◽  
Phytochemical Analysis

Seahorse is one of the marine living  resources usually used as ornamental fish, traditional medicinal materials, and souvenirs. The purpose of the study was to determine the proximate composition of wet and dry seahorses, determine the profile of amino acid hydrolyzate and powder of seahorses, and determines to content of bioactive compounds from the ethanol extract of seahorses on qualitatively. The sample of this study is seahorses obtained from nature. Prviously, seahorses were morphometric identified, subsequently, seahorses were made of the powder, hydrolyzate, and ethanol extract. Several analyzes used were qualitative analysis of proximate, amino acid, and phytochemical analysis. Morphometric identification results indicate that the type is Hippocampus comes. The proximate composition is water content is 66.16 ± 0.33% (wet) and 10.33 ± 0.16% (dry), ash content of 9.55 ± 0.15% (wet) and 9.65 ± 0.16% (dry), lipid content 1.18 ± 0.23% (wet) and 4.89 ± 0.37% (dry), protein content of 22.73 ± 0.17% (wet) and 69.83 ± 0.31% (dry), carbohydrate 0.39 ± 0.23 (wet) and 5.50 ± 0.34 (dry). The amino acid composition both on hydrolyzate and powder comprising 9 essential amino acids are lysine, leucine, isoleucine, phenylalaline, valine, methionine, histidine, arginine, and threonine and 6 non essential amino acids are tyrosine, alanine, glycine, serine, glutamic acid, and aspartic acid. The results of identification of bioactive compounds is flavonoids, triterpenoids, steroids, saponins, and phenol of hydroquinone. Keywords: Hippocampus comes, proximate analysis, amino acid, and bioactive compounds

scholarly journals Evaluation of the Phytochemical and Nutritional Profiles of Cnidoscolus chayamansa (Mc Vaugh) Leaf Collected in Jos, North Central, Nigeria

2021 ◽  
pp. 52-58
Author(s):  
M. K. Jiyil ◽  
R. J. Kutshik ◽  
C. E. Mafuyai ◽  
V. P. Dalong ◽  
D. H. Edward ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Crude Fibre ◽  
Crude Lipid ◽  
North Central ◽  
Cnidoscolus Chayamansa ◽  
Nutritional Profiles

Background: Plants could either be ornamental, medicinal, as well as nutritional, hence there has been a high level of reliance on plants as a whole by both man and animals for survival. Aim of the Study: This study aimed to evaluate the Phytochemical contents and Nutritional Profiles of Cnidoscolus chayamansa  Leaf Collected in Jos, North Central, Nigeria. Study Duration: This study was conducted on 30th June, 2019 at the Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Nigeria. Methodology: The proximate and phytochemical compositions were investigated in accordance with standard procedures. Mineral concentrations were determined by using flame photometer, atomic absorption spectrophotometer, calorimetry.  Crude protein content was determined by Kjeldahl method and amino acid profile were analyzed using Technicon sequential Multi-Sample Amino Acid Analyzer (TSM). Results: The preliminary qualitative phytochemical screening revealed the presence of alkaloid, flavonoids, tanins, saponins, terpenes and steroids, balsam and phenol with the absence of cardiac glycosides, and resin. The proximate analysis showed high carbohydrate (27.48±0.02), crude fibre (25.18±0.02) and protein (18.63±0.01), moderate concentration of moisture content (12.62±0.00) and ashes content (11.68±0.01), low concentration of crude lipid (4.40±0.01). The minerals detected were calcium, magnesium, potassium, sodium, iron, manganese, cobalt, sulphur, aluminium, zinc, molybdenum, and Phosphorus. Seventeen amino acids were analysed; nine essential amino acids namely; Leucine, isoleucine, phenylalanine, trptophane, valine, threonine, arginine, methionine and histidine and Eight non-essential amino acids namely; proline, tyrosine, cysteine, alanine, glutamate, gycine, serine and aspartic. Glutamic and leucine acids were found in higher concentration as 10.14+ 0.016 and 8.99+ 0.000 respectively. Conclusion: This investigation shows that Cnidoscolus chayamansa leaf from Jos, Nigeria contains high medicinal and nutritional compositions which could be exploited for the treatment of diseases as well as nutritional supplements.

scholarly journals Nutritional Potential of Two Insect Species Consumed in Togo: Gnathocera trivittata (Swederus, 1787) and Gnathocera impressa (Olivier, 1789)

2021 ◽  
pp. 24-32
Author(s):  
Fègbawè Badanaro ◽  
Amivi Tete-Benissan ◽  
Kou'santa Amouzou
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Composition ◽  
Essential Amino Acids ◽  
Atomic Absorption Spectrophotometry ◽  
Fiber Content ◽  
Nutritional Values ◽  
Protein Energy ◽  
Micronutrient Malnutrition ◽  
The Individual

Aims:  The objective of this study is to determine the nutritional values of Gnathocera trivittata and Gnathocera impressa in order to promote their consumption for food security. Place and Duration of Study: Samples of G. trivittata and G. impressa were caught in Togo in the three following localities: Kparatao (8°57'151"N; 1°11'838"E), Kpéwa (9°16'978"N; 1°14'149"E) and Soudou (9°21'604"N; 1°21'348"E), between September and December 2013. Methodology: The contents of ash, protein, vitamins as well as lipids were determined. Fiber content was obtained. Minerals were analyzed by atomic absorption spectrophotometry and colorimetry. Fatty acid composition of the lipids was determined by gas chromatography and the amino acid composition was obtained by separation of the individual amino acids using the Biochrom 30+ amino acid analyzer. Results: The results reveal that the average protein content of the insects studied ranged from 59.36 to 61.63%. Average lipid levels ranged from 9.09 to 9.86%. They contain all the essential fatty and amino acids. Their average fiber content fluctuates between 8.35 and 10.05%. Regarding the composition of micronutrients, the species studied are very rich in minerals and vitamins. The ratios of minerals, fatty acids and essential amino acids are balanced. Conclusion:  Given the nutritional potentials of the insects studied, they can contribute significantly to the fight against protein-energy and micronutrient malnutrition in Togo.

Prospects of Using Poultry by-Products in the Technology of Chopped Semi-Finished Products

2018 ◽  
Vol 7 (3.34) ◽  
pp. 495 ◽  
Author(s):  
Gulnar Zhumanova ◽  
Maksim Rebezov ◽  
Bakhytkul Assenova ◽  
Eleonora Okuskhan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Ash Content ◽  
Total Protein Content ◽  
Fat Emulsion ◽  
Microbial Characteristics ◽  
Complete Set ◽  
Sensory Parameters ◽  
By Products

In this paper the technology of preparation the protein-fat emulsion and its effect to sensory, physicochemical and microbial characteristics of meat cutlets are presented. The protein-fat emulsion consists of 75% of the chicken crests, 15% of the vegetable oil and 10% water. The protein, fat and ash content of emulsion are 9.53%, 6.38% and 0.95%, respectively. The amino acid composition of the protein-fat emulsion includes a complete set of essential amino acids - 36.2%. Adding of protein-fat emulsion as an ingredient in meat cutlets increase the total protein content, improves the sensory parameters and consistency of meat cutlets.  

scholarly journals Chemical Analysis of Prosopis Africana (Guill &Perr.) Seeds

2020 ◽  
Vol 3 (2) ◽  
pp. 129-140
Author(s):  
BA Anhwange ◽  
BA Kyenge ◽  
RE Kukwa ◽  
B Ishwa
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Ash Content ◽  
Crude Fibre ◽  
Nutritional Factors ◽  
High Protein Content ◽  
Standard Procedures ◽  
Prosopis Africana

The seeds of Prosopis africana (Guill. & Perr.) were collected and analysed for proximate, anti-nutritional factors, elemental, oxide composition and amino acid profile using standard procedures to evaluate the seeds' nutritional potentials. The results of the proximate composition indicated the following: ash content (3.94%), moisture (8.56%), protein (32.27%), lipids (2.36%), crude fibre (6.75%), and carbohydrate (46.12 %). The results of anti-nutritional factors showed that Prosopis africana seed contained oxalate (0.27 mg/100g), saponin (10.5 mg/100g), alkaloid (7.5 mg/100g), and tannin (16.24 mg/100g). Amino acid profile of the seeds was found to contain; lysine (2.77 mg/100g), methionine (1.86 mg/100g), isoleucine (3.46 mg/100g), leucine (13.26 mg/100g), phenylalanine (4.82 mg/100g), valine (4.13 mg/100g) and non-essential amino acids; arginine (3.62 mg/100g), serine (2.81 mg/100g), aspartic acid (4.58 mg/100g) and glycine (7.12 mg/100g). Elemental composition of the seeds showed potassium (1.397 mg/Kg), calcium (0.405 mg/Kg), Sulphur (1.679 mg/Kg), zinc (0.008 mg/Kg), cadmium (0.002 mg/Kg), phosphorus (0.545 mg/Kg), chlorine (0.021 mg/Kg), manganese (0.01 mg/Kg), silicon (0.202 mg/Kg), and antimony (0.007 mg/Kg); While the oxides composition were: MnO (0.013%), CaO (0.567%), Al O (0.061%), ZnO (0.01%) and SiO (0.433%). The 2 3 2 results of the study revealed that Prosopis africana seed contained high protein content which can be use to supplement the high cost conventional sources. However, the value of anti-nutritional factors suggests that proper processing methods should be adopted before it could be consumed.

scholarly journals Uji Pengaruh Suhu Pengeringan pada Karakteristik Kimia dan Sensori Kaldu Bubuk Kepala Ikan Gabus (Channa striata)

2018 ◽  
Vol 6 (2) ◽  
pp. 97-106
Author(s):  
Akhmad Sobri ◽  
Herpandi Herpandi ◽  
Susi Lestari
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Water Content ◽  
Sensory Analysis ◽  
Essential Amino Acids ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Drying Temperature ◽  
Channa Striata

The purposes of this research was to investigate the proximate content, amino acid content and sensory snakehead fish (Channa striata) powder broth with the effect of drying temperature. The research was conducted on April 2016 to August 2016. The research method was used randomized block design (RBD). Drying temperature treatment used 50 oC, 60 oC and 70 oC. The parameters of this research was proximate analysis such as water content, ash content, protein content, lipid content, and carbohydrate content while for amino acid analysis only to find out the amount of essential amino acids and non-essential course and analytical sensory (appearance, flavor, texture, color and taste). Proximate content gave significant effect on protein content and there was no effect on water content, ash content, fat and carbohydrate content. Gave significant effect on sensory analysis (appearance, flavor, texture, color and taste). The concentration of protein content of the resulting broth powder ranges from 11.99% to 20.94%, there are eight types of essential amino acids and 7 types of non-essential amino acids while sensory analysis produced for sightings range from 3.72 to 4.52, flavor range between 3.8 to 4.32, textures range from 3.52 to 4.4, color range from 1.88 to 3.8 and taste range from 3.84 to 4.4.

Screening of amino acid constituents from date palm fruits

2016 ◽  
Vol 5 (10) ◽  
pp. 4972
Author(s):  
Lata Birlangi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
United Arab Emirates ◽  
Nutritional Value ◽  
Date Palm ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Cultivated Plants ◽  
Palm Fruits ◽  
Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

scholarly journals Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sonia Yun Liu ◽  
Shemil P. Macelline ◽  
Peter V. Chrystal ◽  
Peter H. Selle
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Chicken Meat ◽  
Meat Production ◽  
Accurate Identification ◽  
Reduced Crude Protein

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

scholarly journals Further evidence that mechanisms of host/symbiont integration are dissimilar in the maternal versus embryonic Acyrthosiphon pisum bacteriome

EvoDevo ◽  
2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Celeste R. Banfill ◽  
Alex C. C. Wilson ◽  
Hsiao-ling Lu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acyrthosiphon Pisum ◽  
Essential Amino Acids ◽  
Developmental Time ◽  
Follicular Epithelium ◽  
Future Research ◽  
Buchnera Aphidicola ◽  
Bacterial Endosymbionts ◽  
Asexual Embryogenesis

Abstract Background Host/symbiont integration is a signature of evolutionarily ancient, obligate endosymbioses. However, little is known about the cellular and developmental mechanisms of host/symbiont integration at the molecular level. Many insects possess obligate bacterial endosymbionts that provide essential nutrients. To advance understanding of the developmental and metabolic integration of hosts and endosymbionts, we track the localization of a non-essential amino acid transporter, ApNEAAT1, across asexual embryogenesis in the aphid, Acyrthosiphon pisum. Previous work in adult bacteriomes revealed that ApNEAAT1 functions to exchange non-essential amino acids at the A. pisum/Buchnera aphidicola symbiotic interface. Driven by amino acid concentration gradients, ApNEAAT1 moves proline, serine, and alanine from A. pisum to Buchnera and cysteine from Buchnera to A. pisum. Here, we test the hypothesis that ApNEAAT1 is localized to the symbiotic interface during asexual embryogenesis. Results During A. pisum asexual embryogenesis, ApNEAAT1 does not localize to the symbiotic interface. We observed ApNEAAT1 localization to the maternal follicular epithelium, the germline, and, in late-stage embryos, to anterior neural structures and insect immune cells (hemocytes). We predict that ApNEAAT1 provisions non-essential amino acids to developing oocytes and embryos, as well as to the brain and related neural structures. Additionally, ApNEAAT1 may perform roles related to host immunity. Conclusions Our work provides further evidence that the embryonic and adult bacteriomes of asexual A. pisum are not equivalent. Future research is needed to elucidate the developmental time point at which the bacteriome reaches maturity.

The screening and preliminary construction of quality mutant population for cultivar “Nipponbare” in japonica rice (Oryza sativa)

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Da Zhang ◽  
Jian Wu ◽  
Guan Li ◽  
Chun Shi
Keyword(s):  
Amino Acids ◽  
Oryza Sativa ◽  
Amino Acid ◽  
Mutation Frequency ◽  
Near Infrared ◽  
Essential Amino Acids ◽  
Japonica Rice ◽  
Near Infrared Reflectance ◽  
Mutant Population ◽  
Nonessential Amino Acids

AbstractProgenies of Oryza sativa cv. Nipponbare induced with 0.4% ethyl methane sulphonate (EMS) were screened for quality mutants and the preliminary quality mutant population was constructed in present experiment. A total of 2210 materials were first screened using near infrared reflectance spectroscopy (NIRS) from which 208 quality mutants were obtained for a second screening and then yielded 73 quality mutants including amylase content (AC), gel consistency (GC), gelatinization temperature (GT), protein content (PC), rapid viscosity analysis (RVA) parameters and amino acid contents. The screening yielded 11 PC mutants with a mutation frequency of 4.98‰, followed by 7 rice floury viscosity mutants (3.17‰), 5 AC mutants (2.26‰), 4 chalky mutants, GT and GC mutants (1.81‰), and 2 ASV mutants (0.9‰). The relative contents of 17 kinds of amino acid mutations, including 7 kinds for essential amino acids and 10 kinds for nonessential amino acids were identified. With the variation of 10% as the screening standard, mutants were obtained for lysine and leucine at 0.45‰ and for valine at 4.98‰, but no mutants were found for isoleucine, phenylalanine, threonine. For nonessential amino acids, mutants of glutamic (0.45‰), arginine (3.62‰), alanine (3.17‰), serine (0.45‰), glycine (0.45‰), tyrosine (1.81‰), proline (2.71‰), and histidine (0.45‰) were obtained, but none was found for aspartic, phenylalanine nor threonine. At 100% as the screening standard for methionine and cysteines, the mutation frequency of these two amino acid mutants were 0.9‰ and 4.98‰ respectively. Quality mutants in this preliminary library of rice could play important role in gene function and breeding of rice quality.

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