scholarly journals AMINO ACID PROFILE AND BIOACTIVE COMPOUNDS OF SEAHORSE Hippocampus comes

2018 ◽  
Vol 9 (2) ◽  
pp. 605-617
Author(s):  
Evi Maya Sari ◽  
Mala Nurilmala ◽  
Asadatun Abdullah
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Bioactive Compounds ◽  
Proximate Composition ◽  
Ethanol Extract ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Ash Content ◽  
Phytochemical Analysis

Seahorse is one of the marine living  resources usually used as ornamental fish, traditional medicinal materials, and souvenirs. The purpose of the study was to determine the proximate composition of wet and dry seahorses, determine the profile of amino acid hydrolyzate and powder of seahorses, and determines to content of bioactive compounds from the ethanol extract of seahorses on qualitatively. The sample of this study is seahorses obtained from nature. Prviously, seahorses were morphometric identified, subsequently, seahorses were made of the powder, hydrolyzate, and ethanol extract. Several analyzes used were qualitative analysis of proximate, amino acid, and phytochemical analysis. Morphometric identification results indicate that the type is Hippocampus comes. The proximate composition is water content is 66.16 ± 0.33% (wet) and 10.33 ± 0.16% (dry), ash content of 9.55 ± 0.15% (wet) and 9.65 ± 0.16% (dry), lipid content 1.18 ± 0.23% (wet) and 4.89 ± 0.37% (dry), protein content of 22.73 ± 0.17% (wet) and 69.83 ± 0.31% (dry), carbohydrate 0.39 ± 0.23 (wet) and 5.50 ± 0.34 (dry). The amino acid composition both on hydrolyzate and powder comprising 9 essential amino acids are lysine, leucine, isoleucine, phenylalaline, valine, methionine, histidine, arginine, and threonine and 6 non essential amino acids are tyrosine, alanine, glycine, serine, glutamic acid, and aspartic acid. The results of identification of bioactive compounds is flavonoids, triterpenoids, steroids, saponins, and phenol of hydroquinone. Keywords: Hippocampus comes, proximate analysis, amino acid, and bioactive compounds

scholarly journals Proximate composition, mineral content and amino acid profile of Irvingia gabonensis O’Rorke baill leaf

2016 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Efosa Ewere ◽  
Oboso Etim ◽  
Usunomena Usunobun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximate Composition ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Antinutritional Factors ◽  
World Health ◽  
Isoleucine Leucine ◽  
Irvingia Gabonensis ◽  
Mineral Analyses

Several plants are utilized for medicinal and nutritional purposes. Irvingia gabonensis O’Rorke Baill leaf is used in herbal medicine for treatment of a number of ailments. This study was therefore carried out to investigate the proximate composition, antinutritional factors, mineral composition and amino acid profile of Irvingia gabonensis O’Rorke Baill leaf. The proximate and antinutritional factors analyses were done using standard procedures. The mineral analyses were done using flame photometry, titrimetic method, molybdo vanadate method and atomic absorption spectrophotometry and the amino acid profile was done with the aid of Applied Biosystems PTH amino acid analyzer. Results of proximate analyses were carbohydrates (75.15±1.29 %), protein (11.43±1.07 %), fat (1.99±0.74 %), fibre (4.89±0.61 %), ash (6.71±0.28 %), moisture (5.12±0.03 %) and caloric value (364.30±5.95 Kcal). Antinutrients (phytate, oxalate and cyanide) levels in the leaf were also very low. Results from mineral analyses obtained revealed that the leaf is also a very rich source of calcium, potassium, sodium, magnesium and so on. Compared with the World health organization (WHO) standards, results of the amino acid profile showed that the leaf is very rich in isoleucine, leucine, lysine, phenylalanine, threonine, valine and tyrosine which are nutritionally essential amino acids. Furthermore, extraction of the leaf using ethanol reduced the levels of these amino acids but not below the recommended WHO standard levels for most of the essential amino acids. Irvingia gabonensis O’Rorke Baill leaf is therefore a potential source of key nutrients.

scholarly journals The Proximate Composition, Amino Acid Profile and Chemical Indices in Five Freshwater Fishes from Tiga Dam Reservoir, Nigeria

2019 ◽  
Vol 77 (2) ◽  
pp. 87-92
Author(s):  
Audu Michael Elaigwu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Proximate Composition ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
High Energy ◽  
Dam Reservoir

Abstract The study assesses the proximate composition, amino acid profile and its content and chemical indices of the sun-dried Schilbe mystus, Bagrus bayad, Oreochromis niloticus, Clarias anguillaris and Petrocephalus bane bane from Tiga Dam Reservoir, Nigeria. The proximate composition varied significantly (p<0.05) in all five species of fish and these ranged as follows: Moisture (4.79 - 9.52 g/100 g), Crude Protein (42.20 - 57.71 g/100 g), Ash Content (0.90 - 12.51 g/100 g), Ether Extract (3.41 - 9.93 g/100 g), Crude Fibre (0.62 - 5.08 g/100 g), Nitrogen Free Extract (12.28 - 42.70 g/100 g) and Dry Matter (90.48 - 95.21 g/100 g). The amino acid also differed significantly (p<0.05). The nine essential amino acids found in the five species of fish were Lysine (4.21 - 6.34 g/100 g), Histidine (1.96 - 4.30 g/100 g), Arginine (5.80 - 8.21 g/100 g), Threonine (1.93 - 5.05 g/100 g), Valine (2.91 - 5.53 g/100 g), Methionine (1.74 - 3.80 g/100 g), Isoleucine (2.04 - 3.37 g/100 g), Leucine (3.64 - 7.18 g/100 g) and Phynylalanine (1.90 - 4.23 g/100 g). Whereas, the eight non-essential amino acids included: Serine (2.12 - 5.22 g/100 g), Glutamic acid (13.24 - 16.30 g/100 g), Proline (3.12 - 6.29 g/100 g), Glycine (4.20 - 9.08 g/100 g), Alanine (5.00 - 6.36 g/100 g), Cysteine (0.94 - 1.24 g/100 g), Tyrosine (2.33 - 3.33 g/100 g) and Aspartic acid (6.34 - 11.01 g/100 g). P. bane bane was first in terms of crude protein; S. mystus had the highest lipid and essential amino acid content. Also, C. anguillaris recorded the highest calorific value and best amino acid content. Thus, P. bane bane can serve as a source of animal protein to balance deficiencies in humans. Both S. mystus and C. anguillaris can be used as a nutrient base for high energy food and oil in the food industry.

scholarly journals The Profile of Amino Acids in Sea Horse ((Hippocampus sp)

2021 ◽  
Vol 869 (1) ◽  
pp. 012027
Author(s):  
A A Muhammadar ◽  
Z Nur ◽  
A W Perdana ◽  
M Nasir ◽  
D F Putra ◽  
...  
Keyword(s):  
Analytical Chemistry ◽  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Proximate Analysis ◽  
Ash Content ◽  
Nutritional Values ◽  
Large Size ◽  
Aceh Province ◽  
Gas Chromatography Mass Spectroscopy

Abstract Seahorse (Hippocampus sp.) is a marine fisheries commodity that has high economic and nutritional values. The aims of study were to determine the proximate composition and the profile of the amino acids content in seahorses. The method used refers to the AOAC (Association of Official Analytical Chemistry) for proximate analysis and a GC-MS (Gas Chromatography-Mass Spectroscopy) for the amino acid analysis. This research was done in March 2021. The sea horses were carried out from Simeulue waters, Simeulue district, Aceh Province, Indonesia. Samples were divided into 2 sizes, small size (3.00-5.00 cm) and large size ((5.01-10.00 cm). The analysis was done at laboratorium of Syiah Kuala University. The results of the study was found that the proximate content of the seahorse was water content (6.29%), ash content (25.43%), fat content (0.57%), protein content (39.32%) and carbohydrates ( 28.48%). The amino acid composition of large seahorses and small seahorses consists of four types of essential amino acids, namely leucine, isoleucine, histidine and valine, and six types of non-essential amino acids, namely alanine, glycine, aspartic acid, asparagine, arginine and serine.

scholarly journals Evaluation of the Phytochemical and Nutritional Profiles of Cnidoscolus chayamansa (Mc Vaugh) Leaf Collected in Jos, North Central, Nigeria

2021 ◽  
pp. 52-58
Author(s):  
M. K. Jiyil ◽  
R. J. Kutshik ◽  
C. E. Mafuyai ◽  
V. P. Dalong ◽  
D. H. Edward ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Crude Fibre ◽  
Crude Lipid ◽  
North Central ◽  
Cnidoscolus Chayamansa ◽  
Nutritional Profiles

Background: Plants could either be ornamental, medicinal, as well as nutritional, hence there has been a high level of reliance on plants as a whole by both man and animals for survival. Aim of the Study: This study aimed to evaluate the Phytochemical contents and Nutritional Profiles of Cnidoscolus chayamansa  Leaf Collected in Jos, North Central, Nigeria. Study Duration: This study was conducted on 30th June, 2019 at the Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Nigeria. Methodology: The proximate and phytochemical compositions were investigated in accordance with standard procedures. Mineral concentrations were determined by using flame photometer, atomic absorption spectrophotometer, calorimetry.  Crude protein content was determined by Kjeldahl method and amino acid profile were analyzed using Technicon sequential Multi-Sample Amino Acid Analyzer (TSM). Results: The preliminary qualitative phytochemical screening revealed the presence of alkaloid, flavonoids, tanins, saponins, terpenes and steroids, balsam and phenol with the absence of cardiac glycosides, and resin. The proximate analysis showed high carbohydrate (27.48±0.02), crude fibre (25.18±0.02) and protein (18.63±0.01), moderate concentration of moisture content (12.62±0.00) and ashes content (11.68±0.01), low concentration of crude lipid (4.40±0.01). The minerals detected were calcium, magnesium, potassium, sodium, iron, manganese, cobalt, sulphur, aluminium, zinc, molybdenum, and Phosphorus. Seventeen amino acids were analysed; nine essential amino acids namely; Leucine, isoleucine, phenylalanine, trptophane, valine, threonine, arginine, methionine and histidine and Eight non-essential amino acids namely; proline, tyrosine, cysteine, alanine, glutamate, gycine, serine and aspartic. Glutamic and leucine acids were found in higher concentration as 10.14+ 0.016 and 8.99+ 0.000 respectively. Conclusion: This investigation shows that Cnidoscolus chayamansa leaf from Jos, Nigeria contains high medicinal and nutritional compositions which could be exploited for the treatment of diseases as well as nutritional supplements.

scholarly journals Fortification of Ogi with Whey Increases Essential Amino Acids Content of Fortified Product

2017 ◽  
Vol 2017 ◽  
pp. 1-3
Author(s):  
J. O. Omole ◽  
O. M. Ighodaro ◽  
O. Durosinolorun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximate Composition ◽  
Cheese Whey ◽  
Food Product ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Usual Procedure ◽  
Normal Maize ◽  
Typical Food

The inability of humans to synthesize essential amino acids (EAA) necessitates the need to increase the levels of these nutrient molecules in certain foods in which they are deficient. Maize ogi is a typical food product for both infants and adults in Africa, but with poor EAA content. This study therefore sought to assess the possibility of increasing the EAA content in maize ogi by processing it with cheese whey instead of water. Maize ogi and whey-fortified ogi were prepared by the usual procedure of grain soaking, milling, and drying. Samples from both treatments were subjected to proximate composition and amino acid profile analyses using Waters 616/626 LC (HPLC) instrument. L-lysine, L-trytophan, and L-methionine contents in maize ogi remarkably increased from 0.52, 0.15, and 0.90 mg/100 gm sample, respectively, to 0.90, 240, and 1.320 mg/100 gm sample in whey-fortified ogi. There were also significant increases in other EAA contents of whey-fortified ogi relative to its counterpart (normal maize ogi). The sum increase in EAA contents (9,405 mg) correlates with the increase in protein (1 gm) per gram sample. This study demonstrates that cheese whey increases EAA content in maize ogi and suggests that whey-fortified maize ogi may be a preferred alternative to water processed maize ogi.

scholarly journals Proximate Composition, Amino Acid Profile, and Mineral Content of Four Sheep Meats Reared Extensively in Morocco: A Comparative Study

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Kamal Belhaj ◽  
Farid Mansouri ◽  
Abdessamad Ben moumen ◽  
Marianne Sindic ◽  
Marie-Laure Fauconnier ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximate Composition ◽  
Mineral Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Point Of View ◽  
Iron And Zinc ◽  
Longissimus Lumborum ◽  
Phosphorus And Potassium

This study aimed to compare the organoleptic and nutritional quality of four sheep meats produced in Morocco. This comparison was carried out by analyzing the proximate composition, amino acid profile, and mineral content of meat. The majority of the evaluated parameters were influenced by genetic and geographical factors ( p < 0.05 ). The longissimus lumborum muscle had higher a ∗ value in Timahdite and Ouled-Djellal breeds. The highest values of macroelement were recorded in samples of Beni-Guil meat sampled in the Tendrera region (BGT; 1067.3 mg/100 g), while the highest microelement content was registered in Timahdite meat (5.7 mg/100 g). Iron and zinc were the major identified trace elements, while phosphorus and potassium were the most abundant macroelements. The abundant amino acid was glutamic, while cysteine and methionine were least abundant. The higher essential amino acids index (IEAA) was recorded in the Timahdite sheep meat (162.20, 158.71, 161.02, and 159.65, respectively, for Timahdite, BGT, Ouled-Djellal breeds, and Beni-Guil meat sampled in Ain Beni Mathar region). From a nutritional point of view, the studied meats had a good protein and mineral quality, due to their richness in essential amino acids and microelements. The present study provided new insights on the organoleptic quality and the nutritional value of three Moroccan sheep meats reared in outdoor production system.

scholarly journals PROXIMATE ANALYSIS AND AMINOACID PROFILES OF LEAVES, FLOWERS, PODS, AND SEEDS OF ERYTHRINA EDULIS FROM PERU

2021 ◽  
pp. 30-32
Author(s):  
ADELMO PARRAGA ◽  
JEVIER GONZALES ◽  
ROSARIO PORTALES ◽  
CANDY RUIZ ◽  
ROSARIO ROJAS
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximate Composition ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Human Consumption ◽  
Plant Parts ◽  
Amino Acid Requirements ◽  
Amino Acid Profiles ◽  
Promising Source

Objective: The study aim was to determine the proximate composition and amino acid profiles of seeds, leaves, pods, and flowers of Erythrina edulis that are consumed in Oxapampa, Perú. Methods: Plant parts of E. edulis were analyzed for proximate composition (proteins, carbohydrates, fat, fiber, and ash) according to AOAC methods. Amino acid profiles were determined by HPLC analysis. Results: Fat contents were low (<1.3%) in all four plant parts. The crude fiber was high in leaves and pods (19.9 and 15.1%, respectively). Protein contents in leaves (24.4%) and flowers (23.7%) were higher than that of the pods (19.3%). The limiting amino acids in the seeds were methionine and tryptophan, while the pods were deficient in four amino acids (methionine, tryptophan, histidine, and isoleucine). Of the four plant parts studied, the flowers turned out to be a promising source of protein because they meet most of the amino acid requirements for adults recommended by FAO. Conclusion: The seeds and flowers of E. edulis have a high protein content and a good amino acid profile that makes them recommended for human consumption.

scholarly journals Chemical Analysis of Prosopis Africana (Guill &Perr.) Seeds

2020 ◽  
Vol 3 (2) ◽  
pp. 129-140
Author(s):  
BA Anhwange ◽  
BA Kyenge ◽  
RE Kukwa ◽  
B Ishwa
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Ash Content ◽  
Crude Fibre ◽  
Nutritional Factors ◽  
High Protein Content ◽  
Standard Procedures ◽  
Prosopis Africana

The seeds of Prosopis africana (Guill. & Perr.) were collected and analysed for proximate, anti-nutritional factors, elemental, oxide composition and amino acid profile using standard procedures to evaluate the seeds' nutritional potentials. The results of the proximate composition indicated the following: ash content (3.94%), moisture (8.56%), protein (32.27%), lipids (2.36%), crude fibre (6.75%), and carbohydrate (46.12 %). The results of anti-nutritional factors showed that Prosopis africana seed contained oxalate (0.27 mg/100g), saponin (10.5 mg/100g), alkaloid (7.5 mg/100g), and tannin (16.24 mg/100g). Amino acid profile of the seeds was found to contain; lysine (2.77 mg/100g), methionine (1.86 mg/100g), isoleucine (3.46 mg/100g), leucine (13.26 mg/100g), phenylalanine (4.82 mg/100g), valine (4.13 mg/100g) and non-essential amino acids; arginine (3.62 mg/100g), serine (2.81 mg/100g), aspartic acid (4.58 mg/100g) and glycine (7.12 mg/100g). Elemental composition of the seeds showed potassium (1.397 mg/Kg), calcium (0.405 mg/Kg), Sulphur (1.679 mg/Kg), zinc (0.008 mg/Kg), cadmium (0.002 mg/Kg), phosphorus (0.545 mg/Kg), chlorine (0.021 mg/Kg), manganese (0.01 mg/Kg), silicon (0.202 mg/Kg), and antimony (0.007 mg/Kg); While the oxides composition were: MnO (0.013%), CaO (0.567%), Al O (0.061%), ZnO (0.01%) and SiO (0.433%). The 2 3 2 results of the study revealed that Prosopis africana seed contained high protein content which can be use to supplement the high cost conventional sources. However, the value of anti-nutritional factors suggests that proper processing methods should be adopted before it could be consumed.

scholarly journals Plasma Amino Acids Metabolomics' Important in Glucose Management in Type 2 Diabetes

2021 ◽  
Vol 12 ◽  
Author(s):  
Abdelrahim Alqudah ◽  
Mohammed Wedyan ◽  
Esam Qnais ◽  
Hassan Jawarneh ◽  
Lana McClements
Keyword(s):  
Type 2 Diabetes ◽  
Cardiovascular Disease ◽  
Amino Acids ◽  
Amino Acid ◽  
Fasting Blood Glucose ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Healthy Controls

The perturbation in plasma free amino acid metabolome has been observed previously in diabetes mellitus, and is associated with insulin resistance as well as the onset of cardiovascular disease in this population. In this study, we investigated, for the first time, changes in the amino acid profile in a group of people with and without type 2 diabetes (T2D) with normal BMI, from Jordan, who were only managed on metformin. Twenty one amino acids were evaluated in plasma samples from 124 people with T2D and 67 healthy controls, matched for age, gender and BMI, using amino acids analyser. Total amino acids, essential amino acids, non-essential amino acids and semi-essential amino acids were similar in T2D compared to healthy controls. Plasma concentrations of four essential amino acids were increased in the presence of T2D (Leucine, p &lt; 0.01, Lysine, p &lt; 0.001, Phenylalanine, p &lt; 0.01, Tryptophan, p &lt; 0.05). On the other hand, in relation to non-essential amino acids, Alanine and Serine were reduced in T2D (p &lt; 0.01, p &lt; 0.001, respectively), whereas Aspartate and Glutamate were increased in T2D compared to healthy controls (p &lt; 0.001, p &lt; 0.01, respectively). A semi-essential amino acid, Cystine, was also increased in T2D compared to healthy controls (p &lt; 0.01). Citrulline, a metabolic indicator amino acid, demonstrated lower plasma concentration in T2D compared to healthy controls (p &lt; 0.01). These amino acids were also correlated with fasting blood glucose and HbA1c (p &lt; 0.05). Glutamate, glycine and arginine were correlated with the duration of metformin treatment (p &lt; 0.05). No amino acid was correlated with lipid profiles. Disturbances in the metabolism of these amino acids are closely implicated in the pathogenesis of T2D and associated cardiovascular disease. Therefore, these perturbed amino acids could be explored as therapeutic targets to improve T2D management and prevent associated cardiovascular complications.

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