scholarly journals Chemical composition and in vitro gas production of cassava peel silage using different preservatives

2021 ◽  
Vol 888 (1) ◽  
pp. 012052
Author(s):  
A D Kartika ◽  
Kusmartono ◽  
Mashudi ◽  
PH Ndaru
Keyword(s):  
Chemical Composition ◽  
Rice Bran ◽  
Block Design ◽  
Gas Production ◽  
Randomized Block Design ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Completely Randomized Design ◽  
Cassava Peel

Abstract This study aimed to evaluate the effects of using different types and levels of preservatives on chemical composition and in vitro gas production (IVGP) of cassava peel silage. The following treatments were applied: T0: cassava peel without preservative; T1: T0 + molasses 2%; T2: T0 + molasses 4%; T3: T0 + rice bran 2%; T4: T0 + rice bran 4%; T5: T0 + cassava meal 2% and T6:T0 + cassava meal 4% with 3 replications arranged in a completely randomized design (CRD) for the chemical composition variable and randomized block design (RBD) for IVGP variable. The results showed that treatments significantly affected (P<0.01) DM, OM, and EE contents, significantly affected (P<0,05) IVGP but did not significantly affect CP, CF contents, potential, and rate of gas production (P>0.05). Treatment T5 seems to have higher values of IVGP and ME (159.1±7.19 ml/500 mgDM; 11.1±0.36MJ/kg DM, respectively) compared to the other treatments that might have related to a higher OM available for in vitro fermentation process. It can be concluded that the use of cassava meal at 2% level can be considered as the most ideal preservative to use to produce a high-quality cassava peel silage.

scholarly journals Rumen fermentation kinetics as impacted by red amaranth (Amaranthus cruentus, L) leaf powder using in vitro gas production technique

2020 ◽  
Author(s):  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Metha Wanapat
Keyword(s):  
Rumen Fermentation ◽  
Gas Production ◽  
Amaranthus Cruentus ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Two Factors ◽  
Dry Matter Degradation ◽  
Leaf Powder ◽  
Completely Randomized Design

Abstract Background A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate. Results Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation. Conclusion Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.

scholarly journals In Vitro protein digestibility and fermentability of mulberry (Morus alba)-Leucaena foliage mixed feed

2016 ◽  
Vol 21 (1) ◽  
pp. 9
Author(s):  
Dwi Yulistiani ◽  
Z. A. Jelan ◽  
J. B. Liang
Keyword(s):  
Leucaena Leucocephala ◽  
Protein Digestibility ◽  
Gas Production ◽  
Mixed Feed ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Production Study ◽  
Completely Randomized Design

<p class="abstrak2">This experiment was carried out to determine the effect of mulberry-leucaena foliage mixed feed on protein digestibility and VFA production using an in vitro gas production study. Mulberry was mixed with one of 2 leucaena varieties (<em>Leucaena leucocephala</em> hybrid and <em>Leucaena leucocephala</em> local) at 3 levels (0, 25 and 50%). Study was conducted in completely randomized design. Mulberry foliage, leucaena and mixtures of mulberry-leucaena were incubated for 24 hours in glass syringes. Parameter recorded were gas production, in vitro true dry matter digestibility (IVTDMD), in vitro N digestibility (IVND) and VFA production. Results of the study showed that supplementation of leucaena to mulberry decreased IVOMD, gas production and protein digestibility in the rumen buffered medium. The mixture of mulberry and leucaena hybrid at ratio 50% resulted in the lowest IVND than other treatment. However it increased protein digestibility in acid pepsin incubation as an estimate of protein availability in intestine. Gas production decreased in mulberry leucaena mixtures was followed by decreased total VFA production. Tannin derived from leucaena hybrid supplementation to mulberry at ratio 1 : 1 was most effective to decrease protein digestion in the rumen and to increase protein digestibility in acid pepsin incubation. In conclusion mixing of hybrid leucaena hybrid with mulberry foliage was able to protect protein degradation from mulberry in the rumen.</p>

scholarly journals Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Block Design ◽  
Gas Production ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
In Vitro Gas Production

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

scholarly journals Effect of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production, and rumen fermentation

2021 ◽  
Vol 888 (1) ◽  
pp. 012053
Author(s):  
A D Saputra ◽  
Kusmartono ◽  
Mashudi ◽  
P H Ndaru
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Cassava Leaves ◽  
In Vitro Gas Production ◽  
Cassava Peel ◽  
Different Levels

Abstract This study was designed to evaluate effects of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production (IVGP) and rumen fermentation. The treatments applied were: A=cassava peel (20%)+ cassava meal (70%)+cassava leaves (5%)+moringa leaves (5%); B=cassava peel (20%)+ cassava meal (60%)+cassava leaves (10%)+moringa leaves (10%); C=cassava peel (20%)+ cassava meal (50%)+cassava leaves (15%)+moringa leaves (15%) cassava meal; D=cassava peel (20%)+ cassava meal (40%)+cassava leaves (20%)+moringa leaves (20%); E=cassava peel (20%)+ cassava meal (30%)+cassava leaves (25%)+moringa leaves (25%) with 3 replications arranged in a Randomized Block Design (RBD). The result showed that the increasing levels of cassava meal in the ration significantly increased organic matter (OM) and nitrogen-free extract (NFE) content (P<0.05), but reduced crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents. Similarly, a significant increased (P<0.01) were found in values of cumulative in vitro gas production, dry matter and organic matter digestibility, but decreased NH3 concentration (P<0.05) due to the increased of cassava meal. It is concluded that increasing levels of cassava meal in concentrate has led to higher OM content of the ration and being available for rumen fermentation.

scholarly journals Kajian Konsentrasi Iaa dan Bap pada Multiplikasi Pisang Raja Bulu In Vitro dan Aklimatisasinya

2018 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Eddy Tri Haryanto ◽  
Retno Bandriyati Arniputri ◽  
Endang Setia Muliawati ◽  
Ellyvia Trisnawati
Keyword(s):  
Block Design ◽  
Cost Effective ◽  
Culture Technique ◽  
Tropical Fruit ◽  
Randomized Block Design ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
Leaf Appearance

<p>Banana is a tropical fruit plant that popular in people. The availability of Raja Bulu banana seedling insufficient to cover the needs. Problems in commercial banana cultivation is the least availability of clonal superior seeds. Multiplication in tissue culture technique can potentially overcome all limitations of Raja Bulu conventional seedling. So that, the objective of this research was to multiplicate an amount of Raja Bulu banana shoot using plant growth regulator in MS medium to initially produce shoot organs. The multiplication was done by Completely Randomized Design with 2 factor of IAA (0; 0,5; 1 ppm) and BAP (0, 2, 4 ppm). The data were analyzed by ANOVA and the mean compared using Duncan’s pairwise comparisons at p = 0,05. The acclimatization was design by Completely Randomized Block Design with 3 different media of palm fiber, bagasse, and banana pseudo stem then analyzed by ANCOV. The results showed there was interaction of IAA and BAP on variables at the appearance of shoots and the leaf appearance in multiplication. The medium without IAA and contained BAP 2 ppm showed the highest mean number of shoot but it was not cost effective with other treatment in this multiplication. Giving IAA 0,5 ppm with BAP 4 ppm accelerated when the shoots appear and when the leaves appear on multiplication Raja Bulu banana. There was no significant interaction or influence of IAA and BAP on plant height, leaf number, and pseudo stem diameter as acclimatization variable with 3 medium used. The percentage of acclimatization success is 76,5%.</p>

Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

2021 ◽  
pp. 1-10
Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Gas Production ◽  
Rapid Decline ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Acid Detergent Fibre ◽  
Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

scholarly journals Okra seedlings production in protected environment, testing substrates and producing fruits in field

2013 ◽  
Vol 31 (1) ◽  
pp. 08-14 ◽  
Author(s):  
Edilson Costa ◽  
Taynara G de Souza ◽  
Gleciane de L Benteo ◽  
Katiane SS Benett ◽  
Cleiton GS Benett
Keyword(s):  
Block Design ◽  
Joint Analysis ◽  
State University ◽  
Mato Grosso ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hot Weather ◽  
Favorable Conditions ◽  
Mato Grosso Do Sul

The okra grows well in hot weather, finding favorable conditions for development in Brazil. We evaluated the production of okra seedlings and productivity. The experiment was conducted in the experimental area of the State University of Mato Grosso do Sul, in Aquidauana. In the seedling phase we tested substrates (100% triturated cassava stems, 75% triturated cassava stems and 25% vermiculite, 50% triturated cassava stems and 50% vermiculite, 25% triturated cassava stems and 75% vermiculite and 100% vermiculite). These substrates were placed in two protected environments (nursery with black screen, 50% of shading, and aluminized screen, 50% of shading). Each environment was considered an experiment conducted in completely randomized design with eight replications. After that we realized joint analysis. In the field, a total of 10 treatments generated from combinations of environments and substrates (2 environments x 5 substrates) were distributed in a randomized block design with four replications. The average proportions of cassava stems and vermiculite may be suitable for okra seedlings. The results of shoot and total dry phytomass indicate that the aluminized screen can be recommended for okra seedlings production when the substrate with a 1:1 ratio is used. Seedlings grown on higher percentages of vermiculite anticipate production, while seedlings produced only in cassava stems tend to slow it down. The aluminized screen tends to increase the yield of okra seedlings. Average proportions of vermiculite and cassava stems for seedling production under aluminized screen, provide more fruits and yield of okra in Aquidauana.

scholarly journals Evaluation of nutrient content and in-vitro gas production of complete feed based on corn stover (Zea mays) supplemented by mimosa powder and myristic acid

2020 ◽  
Vol 18 (2) ◽  
pp. 191
Author(s):  
Muchamad Muchlas ◽  
Siti Chuzaemi ◽  
Mashudi Mashudi
Keyword(s):  
Carbon Dioxide ◽  
Zea Mays ◽  
Corn Stover ◽  
Myristic Acid ◽  
Block Design ◽  
Gas Production ◽  
Total Carbon ◽  
Methane Gas ◽  
In Vitro Gas Production

<p class="MDPI17abstract"><strong>Objective: </strong>The purpose of this research was to evaluate the effect supplementation of mimosa powder as a source of condensed tannins and a single fatty acid, myristic acid, in a complete feed based on corn stover (<em>Zea mays</em>) using the in-vitro gas production method. This research has been carried out at the Animal Nutrition and Food Laboratory, Faculty of Animal Husbandry, Brawijaya University. The time of the research was conducted in August until December 2019.</p><p class="MDPI17abstract"><strong>Methods: </strong>The experimental design used randomized complete block design by ANOVA consisting four treatments and three replications which were P1= a complete feed based on corn stover (<em>Zea mays</em>) as control Diet (CD) (40% corn stover + 60 % concentrate), P2= (CD) + Mimosa Powder(MP) 1.5 %/kg DM + myristic acid (MA)2% /kg DM, P3= CD + MP 1.5 % /kg DM + MA 3% /kg DM, and P4= CD + MP 1.5 %/kg DM + MA 4 %/kg DM.</p><p class="MDPI17abstract"><strong>Results: </strong>The results showed that the treatments affected total gas production (p&lt;0.01). The highest value for total gas production was found in P1 (86.67 ml/500 mg DM) and the lowest was found in P3 (73.30 ml/500 mg DM). The results showed that gas production decreased concurrently with the increase of MA level. In vitro methane gas and carbon dioxide production was showed different (p&lt;0.05) from the control treatment. The lowest concentration of methane production was in P4 (82863.07 ppm) and the highest concentration was in treatment P1 86530.89 ppm. The highest total carbon dioxide content was P1 (436711.57 ppm) and the lowest concentration was P3 (350287.72 ppm).</p><p class="MDPI17abstract"><strong>Conclusions: </strong>The results of the research concluded that the addition of mimosa powder and 3 different levels of myristic acid in a complete feed based on corn stover can increase the nutritional value of a complete feed and reduce the production of methane gas.</p>

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