scholarly journals Kajian Konsentrasi Iaa dan Bap pada Multiplikasi Pisang Raja Bulu In Vitro dan Aklimatisasinya

2018 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Eddy Tri Haryanto ◽  
Retno Bandriyati Arniputri ◽  
Endang Setia Muliawati ◽  
Ellyvia Trisnawati
Keyword(s):  
Block Design ◽  
Cost Effective ◽  
Culture Technique ◽  
Tropical Fruit ◽  
Randomized Block Design ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
Leaf Appearance

<p>Banana is a tropical fruit plant that popular in people. The availability of Raja Bulu banana seedling insufficient to cover the needs. Problems in commercial banana cultivation is the least availability of clonal superior seeds. Multiplication in tissue culture technique can potentially overcome all limitations of Raja Bulu conventional seedling. So that, the objective of this research was to multiplicate an amount of Raja Bulu banana shoot using plant growth regulator in MS medium to initially produce shoot organs. The multiplication was done by Completely Randomized Design with 2 factor of IAA (0; 0,5; 1 ppm) and BAP (0, 2, 4 ppm). The data were analyzed by ANOVA and the mean compared using Duncan’s pairwise comparisons at p = 0,05. The acclimatization was design by Completely Randomized Block Design with 3 different media of palm fiber, bagasse, and banana pseudo stem then analyzed by ANCOV. The results showed there was interaction of IAA and BAP on variables at the appearance of shoots and the leaf appearance in multiplication. The medium without IAA and contained BAP 2 ppm showed the highest mean number of shoot but it was not cost effective with other treatment in this multiplication. Giving IAA 0,5 ppm with BAP 4 ppm accelerated when the shoots appear and when the leaves appear on multiplication Raja Bulu banana. There was no significant interaction or influence of IAA and BAP on plant height, leaf number, and pseudo stem diameter as acclimatization variable with 3 medium used. The percentage of acclimatization success is 76,5%.</p>

scholarly journals Chemical composition and in vitro gas production of cassava peel silage using different preservatives

2021 ◽  
Vol 888 (1) ◽  
pp. 012052
Author(s):  
A D Kartika ◽  
Kusmartono ◽  
Mashudi ◽  
PH Ndaru
Keyword(s):  
Chemical Composition ◽  
Rice Bran ◽  
Block Design ◽  
Gas Production ◽  
Randomized Block Design ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Completely Randomized Design ◽  
Cassava Peel

Abstract This study aimed to evaluate the effects of using different types and levels of preservatives on chemical composition and in vitro gas production (IVGP) of cassava peel silage. The following treatments were applied: T0: cassava peel without preservative; T1: T0 + molasses 2%; T2: T0 + molasses 4%; T3: T0 + rice bran 2%; T4: T0 + rice bran 4%; T5: T0 + cassava meal 2% and T6:T0 + cassava meal 4% with 3 replications arranged in a completely randomized design (CRD) for the chemical composition variable and randomized block design (RBD) for IVGP variable. The results showed that treatments significantly affected (P<0.01) DM, OM, and EE contents, significantly affected (P<0,05) IVGP but did not significantly affect CP, CF contents, potential, and rate of gas production (P>0.05). Treatment T5 seems to have higher values of IVGP and ME (159.1±7.19 ml/500 mgDM; 11.1±0.36MJ/kg DM, respectively) compared to the other treatments that might have related to a higher OM available for in vitro fermentation process. It can be concluded that the use of cassava meal at 2% level can be considered as the most ideal preservative to use to produce a high-quality cassava peel silage.

scholarly journals Okra seedlings production in protected environment, testing substrates and producing fruits in field

2013 ◽  
Vol 31 (1) ◽  
pp. 08-14 ◽  
Author(s):  
Edilson Costa ◽  
Taynara G de Souza ◽  
Gleciane de L Benteo ◽  
Katiane SS Benett ◽  
Cleiton GS Benett
Keyword(s):  
Block Design ◽  
Joint Analysis ◽  
State University ◽  
Mato Grosso ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hot Weather ◽  
Favorable Conditions ◽  
Mato Grosso Do Sul

The okra grows well in hot weather, finding favorable conditions for development in Brazil. We evaluated the production of okra seedlings and productivity. The experiment was conducted in the experimental area of the State University of Mato Grosso do Sul, in Aquidauana. In the seedling phase we tested substrates (100% triturated cassava stems, 75% triturated cassava stems and 25% vermiculite, 50% triturated cassava stems and 50% vermiculite, 25% triturated cassava stems and 75% vermiculite and 100% vermiculite). These substrates were placed in two protected environments (nursery with black screen, 50% of shading, and aluminized screen, 50% of shading). Each environment was considered an experiment conducted in completely randomized design with eight replications. After that we realized joint analysis. In the field, a total of 10 treatments generated from combinations of environments and substrates (2 environments x 5 substrates) were distributed in a randomized block design with four replications. The average proportions of cassava stems and vermiculite may be suitable for okra seedlings. The results of shoot and total dry phytomass indicate that the aluminized screen can be recommended for okra seedlings production when the substrate with a 1:1 ratio is used. Seedlings grown on higher percentages of vermiculite anticipate production, while seedlings produced only in cassava stems tend to slow it down. The aluminized screen tends to increase the yield of okra seedlings. Average proportions of vermiculite and cassava stems for seedling production under aluminized screen, provide more fruits and yield of okra in Aquidauana.

scholarly journals Advanced lines of Jalapeño pepper with potential for mechanical harvesting

2019 ◽  
Vol 49 (2) ◽  
Author(s):  
Lucas Matias Gomes ◽  
Cláudia Silva da Costa Ribeiro ◽  
Carlos Francisco Ragassi ◽  
Lorena Sousa Silva ◽  
Francisco José Becker Reifschneider
Keyword(s):  
Field Experiment ◽  
Plant Height ◽  
Block Design ◽  
Field Evaluation ◽  
Fruit Characteristics ◽  
Selected Lines ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Plant Characteristics ◽  
Completely Randomized Design

ABSTRACT: The objective of this study was to evaluate the plant and fruit characteristics in advanced lines of Jalapeño pepper for mechanical harvesting. Thus, two experiments were carried out in the field. Experiment I (2015) was conducted as a randomized block design (three replications and eight plants per plot) and Experiment II (2016) in a completely randomized design (five replications and ten plants per plot). In 2015, lines CNPH 30,112, CNPH 30,118, CNPH 30,147, CNPH 30,159, CNPH 30,183, and CNPH 30,245 were evaluated, together with the control ‘BRS Sarakura’. Significant differences were detected for fruit and plant characteristics (P<0.05). Lines CNPH 30,118, CNPH 30,147, CNPH 30,159, and CNPH 30,245 were selected based on plant height and height of the first bifurcation, earliness, and yield. They were evaluated again in 2016, together with ‘BRS Sarakura’ and ‘Jalapeño Plus F1’ as controls. In 2016, CNPH 30,159 and CNPH 30,118 were selected due to their potential for mechanical harvesting. They were evaluated for height of the first bifurcation (>10cm), large fruits (11 cm x 4 cm), high pulp firmness (11 N), wall thickness (about 5mm), and content of capsaicinoid substances (>50,000 SHU). Selected lines are undergoing additional field evaluation for mechanical harvesting with the Etgar Moses 1010 pepper harvester.

scholarly journals Health quality and germination of Butia capitata seeds treated with bactericide and fungicide

2016 ◽  
Vol 42 (4) ◽  
pp. 303-307
Author(s):  
Vander Rocha Lacerda ◽  
◽  
Diemesson San Tiago Mendes ◽  
Fernando da Silva Rocha ◽  
Maria de Fátima Gonçalves Fernandes ◽  
...  
Keyword(s):  
Block Design ◽  
Transparent Plastic ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Seed Health ◽  
High Incidence ◽  
Health Quality ◽  
Completely Randomized Design ◽  
Test Treatments ◽  
And Control

ABSTRACT Propagation of “coquinho-azedo” (Butia capitata) is limited due to dormancy and high incidence of fungi in its seeds. The aim of this study was to evaluate the effect of treating “coquinho-azedo” seeds with different concentrations of bactericide and/or fungicide on the incidence of fungi and on the germination of seeds. Thus, B. capitata seeds were immersed in four doses of the bactericide kasugamycin (0, 1.5, 3.0 and 4.5 mL/L) and/or in four concentrations of the fungicide carbendazim+ thiram (0, 2.92, 5.84 e 8.75 mL/Kg), totaling 16 treatments. After the treatments, the operculum was removed from the seeds, which were placed on trays containing autoclaved sand, covered with transparent plastic film and kept in the nursery. In the germination test, treatments were distributed in randomized block design, factorial arrangement of 4x4 (bactericidal doses x fungicide doses), with four replicates of 50 seeds per plot. For the test of seed health quality, the Blotter test was employed in completely randomized design, using 20 replicates and 10 seeds. Health quality and germination were evaluated at 15 and 40 days after the treatment of seeds, respectively. There was no interaction between the bactericide and the fungicide on germination and control of fungi associated with seeds. However, there was an effect of fungicide concentrations on the evaluated parameters. We observed a negative correlation between the concentrations of the fungicide carbendazim + thiram and fungal incidence, and a positive correlation between the fungicide concentrations and seed germination. Therefore, fungicide treatment reduces the fungal incidence and provides better germination of B. capitata seeds.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals KUALITAS KIMIA DAN ORGANOLEPTIK BURGER IKAN TUNA YANG DISUBTITUSI DENGAN TEPUNG BUAH MENGKUDU (Morinda citrifolia)

2018 ◽  
Vol 3 (1) ◽  
pp. 1-8
Author(s):  
Evi Huzaibah ◽  
Asrawaty Asrawaty ◽  
Minarny Gobel
Keyword(s):  
Moisture Content ◽  
Block Design ◽  
Experimental Treatment ◽  
Ash Content ◽  
Morinda Citrifolia ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Effect Of Treatment ◽  
Completely Randomized Design ◽  
Tapioca Flour

The purpose of this research is to know the effect of addition of noni flour as substitution of tapioca flour to protein content, moisture content, and ash content and organoleptic nature of tuna burger. This study used Completely Randomized Design (RAL) and Randomized Block Design (RAK). The experimental treatment were: B1 (0% noni flour + 15% tapioca flour), B2 (2.5% noni flour + 12.5% tapioca flour), B3 (5% noni flour + 10% tapioca flour), B4 7.5% noni flour + 7.5% tapioca flour). To know the effect of treatment, then the analysis of variance and if the treatment has real effect continued with BNT advanced test. Based on the result of the research, it can be concluded that the addition of noni flour as substitution of tapioca flour to 7.5% can increase the protein tuna burger protein by 14.87%, moisture content 71.07% and ash content 2.07%, and aroma, taste, color, and texture are relatively the same on all treatments

scholarly journals EMBRIOGENESIS SOMATIK DARI KALUS MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PEMBERIAN BAP DAN MADU SECARA IN VITRO

2019 ◽  
Vol 12 (1) ◽  
pp. 8-17
Author(s):  
Tirtha Juliana ◽  
Mayta Novaliza Isda ◽  
Dyah Iriani
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Formation ◽  
Block Design ◽  
Culture Technique ◽  
Tropical Fruits ◽  
Garcinia Mangostana ◽  
Randomized Block Design ◽  
Murashige Skoog ◽  
Callus Volume

AbstrakGarcinia mangostana L. dikenal dengan sebutan queen of the tropical fruits. Buah manggis terbentuk secara apomiksis yang bersifat rekalsitran. Salah satu cara perbanyakan tanaman manggis adalah dengan teknik kultur in vitro melalui embriogenesis somatik. Embriogenesis somatik manggis dilakukan dengan pembentukan kalus terlebih dahulu. Penelitian ini bertujuan untuk menentukan konsentrasi terbaik BAP dan madu secara tunggal serta kombinasinya dalam pembentukan embriogenesis somatik pada kalus biji manggis asal Bengkalis. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan pemberian konsentrasi BAP (3 dan 7 mg/L) dan madu (3, 6, dan 9 mL/L), secara baik tunggal maupun kombinasi, pada media Murashige-Skoog (MS) dengan 3 ulangan. Hasil penelitian menunjukkan bahwa pemberian BAP dan madu dalam seluruh perlakuan tersebut berpengaruh terhadap pembentukan fase-fase embriogenesis somatik kalus manggis. Konsentrasi terbaik dalam pembentukan fase embriogenesis somatik diperoleh dari perlakuan 3 mg/L BAP + 9 mL/L madu dengan presentase pembentukan kalus 100%, waktu muncul kalus 10,67 hst, volume kalus 1,33 dan adanya fase embriogenesis somatik berupa globular, hati, dan torpedo.Abstract Garcinia mangostana L. was known as the queen of the tropical fruits. Mangosteen was formed by apomixis which is recalcitrant. One of the methods of mangosteen propagation is by using a tissue culture technique through somatic embryogenesis. Mangosteen somatic embryogenesis occurs preceded by callus formation. This study aimed to determine the best concentration of BAP and honey in single as well as in combination for the formation phase of somatic embryogenesis in the callus of mangosteen from Bengkalis. The study used a randomized block design with the addition of BAP (3 and 7 mg/L) and honey (3; 6; and 9 mL/L) either single or combination in Murashige-Skoog (MS) medium with 3 replications. The results of this study indicated that the addition of BAP and honey in all treatments affected the phases of somatic embryogenesis of  mangosteen callus. The best concentration in the formation of somatic embryogenesis was obtained from, the treatment of 3 mg/L BAP + 9 mL/L which produced 100% of callus formation, with callus emergence time of 10.67 days after plantation, callus volume of 1.33 and the presence of somatic embryogenesis in the form of globular, heart, and torpedo.

scholarly journals Diseños experimentales e investigación científica

2017 ◽  
Vol 4 (8) ◽  
Author(s):  
M. H. Badii, ◽  
M. Castillo Rodríguez ◽  
A. Wong ◽  
P. Villalpando
Keyword(s):  
Block Design ◽  
Latin Square ◽  
Real Data ◽  
Experimental Designs ◽  
Design Experiment ◽  
Experiment Research ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Plot Design

Key words: Design, experiment, research, scienceAbstract. The basics of the experimental designs are noted. Different features of common types of experimental designs such as the completely randomized design, the randomized block design, the Latin Square design, the split plot design and the factorial design are described. Each experimental design is illustrated by an example using real data. The application of experimental designs to the scientific research is discussed.Palabras claves: Ciencia, diseño, experimento, investigaciónResumen. Se describen los fundamentos de los diseños experimentales. Se explican las distintas características de los diseños experimentales del uso común, tales como el diseño completamente aleatorio, e diseño de bloques al azar, el diseño de cuadro latino, el diseño de parcelas divididas y el de factorial. Para cada diseño se presenta un ejemplo con los datos reales del campo. Se discute la aplicación de estos diseños en relación con la investigación científica.

scholarly journals IDENTIFIKASI FENOTIPIK GALUR-GALUR KEDELAI TERHADAP KETAHANAN SERANGAN HAMA ULAT GRAYAK (SPODOPTERA LITURA F.)

2016 ◽  
Vol 15 (2) ◽  
pp. 180
Author(s):  
Gatut Wahyu Anggoro Susanto ◽  
Moh. Muchlish Adie
Keyword(s):  
Spodoptera Litura ◽  
Block Design ◽  
Petri Dish ◽  
Breeding Line ◽  
Biological Aspect ◽  
Breeding Lines ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Soybean Resistance ◽  
Completely Randomized Design

Phenotypic identification of soybean lines against armyworm pest resistance (Spodoptera litura F). This research consisted of two parts which aimed to find out the intensity of damage to the leaves and the influence of soybean on the biological aspect of armyworms. The materials tested were Shr/W-C-60, Aochi/Wil-60, 9837/K-D-8-185, 9837/K-D-3-185-95, W/9837-D- 6-220, 9837/K-D-3-185-82, 9837/W-D-5-211, GI, G100H breeding lines and Wilis varieties. The research was conducted at a Balitkabi screenhouse in February 2011, using randomized block design and each treatment was replicated three times. The planting media were plastic pots (diameter 18 cm) filled with earth, two seeds per pot were planted and intensively raised. When the plants were 27 days old after planting, at each replication consisting of 10 materials, they were covered with gauze cages (2 x 2 x 2 m). Then the plants in each pot was infested with 10 instar I armyworm larvae. The other part of research was carried out in Balitkabi Breeding Improvement Laboratory. For testing purposes, there was a need for a third nodal leaf of soybean aged 27 days after planting in each tested material. The research used a completely randomized design each treatment was replicated three times. One nodal leaf in each material was placed in a petri dish 15 cm in diameter, which was coated with moist filter paper, and this was later called treatment. Each treatment was infested with one instar I armyworm larva. The result of research indicated that an assessment of soybean resistance to armyworms could be made from the density of the trichome and/or the length of the trichome on the leaves. The G100H breeding line was found to be resilient with its characteristics of having dense trichome (25/4 mm2) and long trichome (1.1 mm).

scholarly journals Physical quality and digestibility in vitro determination of green pellet concentrate based on Indigofera zollingeriana

2018 ◽  
Vol 22 (3) ◽  
pp. 114 ◽  
Author(s):  
Andi Tarigan ◽  
Simon P. Ginting ◽  
I. I. Arief ◽  
D. A. Astuti ◽  
L. Abdullah
Keyword(s):  
Block Design ◽  
Physical Quality ◽  
Green Pellet ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Indigofera Zollingeriana ◽  
Total Bacteria

<p>This study aims to determine the physical quality and digestibility of green pellet concentrate based on <em>I.</em><em> zollingeriana</em>. The composition of green pellet concentrate feed contains <em>I. zollingeriana</em> and <em>C.</em><em> calothyrsus</em> in the following combinations: 1) <em>I.</em><em> zollingeriana</em> /<em>C.</em><em> calothyrsus</em> (90/0; R0), 2) <em>I.</em><em> zollingeriana</em>/<em>C. calothyrsus</em> (75/15; R1), 3) <em>I.</em><em> zollingeriana</em>/<em>C. calothyrsus</em> (60/30; R2) and 4<em>) I.</em><em> zollingeriana</em> / <em>C. calothyrsus</em> (45/45; R3). The physical quality study of green pellet concentrate was performed using a completely randomized design with four types of green concentrate as treatments of three replications. Green pellet concentrate studies conducted in vitro rumen fermentation were performed using in a  randomized block design with four treatments and three sources of liquid rumen as a block. The results of the experiments conducted with pellet criteria containing <em>I.</em><em> zollingeriana/C. calothyrsus </em>composition (60/30; R2) have the best physical character (water activity = 0.58; particles size = 12.69 mm; degree value angles = 21.01<sup>o</sup>; collision endurance = 99.78%; endurance friction = 90.42 %; bulk density = 590 kg/m3) compared to the other treatments. The quality of diet based on digestibility in vitro, dry matter and organic material having a pattern equal to R0 is higher (P&lt;0.05) compared with other treatments, while the rough digestibility of In Vitro protein is seen R0, R1, and R2 (P&gt;0.05), but (P&lt;0.05) was higher than the treatment of R3. There was no difference of pH and total bacteria on green pellet concentrate type. The treatment of R3 has the lowest NH3, VFA and total gas concentration (P&lt;0.5) compared with R2, R1 and R0. It was concluded that concentrate green pellet of <em>I.</em><em> zollingeriana</em> composition (60%) and <em>C.calothyrsus</em> (30%) had better physical and nutritional quality.</p>

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