scholarly journals Optimization of annealing temperature for amplification of EhoscnOla locus in pranajiwa (Euchresta horsfieldii) plant collected from mountains, urban and coastal areas in Bali

2021 ◽  
Vol 913 (1) ◽  
pp. 012059
Author(s):  
D Silalahi ◽  
I G P Wirawan ◽  
M M V Sasadara
Keyword(s):  
Medicinal Plant ◽  
Dna Barcoding ◽  
Annealing Temperature ◽  
Rare Plant ◽  
Optimal Temperature ◽  
Different Temperatures ◽  
Temperature Optimization ◽  
Reverse Primer ◽  
Total Dna ◽  
Forward Primer

Abstract Pranajiwa plant is a medicinal plant that grows wildly and is classified as a rare plant. Currently, its existence is increasingly threatened. Pranajiwa grows around Indonesia and is known with several scientific names and morphological features due to unclear identification. Molecular identification is recommended to clarify its species. DNA Barcoding is considered the suitable method to identify pranajiwa plant molecularly. The purpose of this study was to optimized the PCR annealing temperature of EhcSnOla locus barcoding marker of pranajiwa plants collected from the coastal (Jimbaran), urban (Renon), and mountain (Bedugul) areas, representing three different areas in Bali. Research procedures include total DNA extraction, PCR procedure, and electrophoresis. The primers used in this study were EhoScnOla forward primer and EhoscnOla reverse primer. Five different temperatures were used for annealing temperature optimization: 51°C, 52°C, 55°C, 57°C, and 60°C. The result showed that all temperatures produced a clear, thick, and single electrophoresis band, indicating that all temperatures were suitable for the annealing temperature and the most optimal temperature is in the Mountains sample (Bedugul) which is 60°C. The Jimbaran, Renon, and Bedugul samples produced 882, 820, and 889 bp, respectively. EhcSnOla locus can be used as the barcoding marker to identify pranajiwa molecularly.

scholarly journals IDENTIFIKASI POLIMORFISME GEN GH (GROWTH HORMONE) SAPI BALI DENGAN METODE PCR-RFLP

2006 ◽  
Vol 12 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Sri Rahayu ◽  
Sutiman Bambang Sumitro ◽  
T Susilawati ◽  
Soemarno Soemarno
Keyword(s):  
Growth Hormone ◽  
Restriction Enzyme ◽  
Growth Hormone Gene ◽  
Salting Out ◽  
Pcr Product ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Total Dna ◽  
Polymerase Chain ◽  
Forward Primer

This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene. The DNA was isolated from blood samples by salting out method. Total DNA were amplified with forward primer, 5’-TAGGGGAGGGTGGAAAATGGA-3’ and reverse primer, 5’-GACACCTACTCAGACAATGCG-3’. The PCR product was digested by HaeIII restriction enzyme. Result of the amplification was a specific single band with fragment 450 bp. Restriction with HaeIII restriction enzyme resulted four kinds of haplotype. Haplotype I was not cut by HaeIII restriction enzyme. Haplotype II were cut into two, 225 bp and 150 bp,. Haplotype III were cut into three size, 400 bp, 225 bp and 150 bp. Haplotype IV were cut into five fragments 450 bp, 400 bp, 275 bp, 225 bp and 150 bp.

scholarly journals OPTIMASI SUHU ANNEALING UNTUK EMPAT PRIMER RAPD PADA KACANG HIJAU (Vigna radiata L.)

2019 ◽  
Vol 34 (1) ◽  
pp. 41-46
Author(s):  
Herman Herman ◽  
Martupa Nainggolan ◽  
Dewi Indriyani Roslim
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Annealing Temperature ◽  
Random Amplified Polymorphic Dna ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Temperature Optimization ◽  
Total Dna

Determination of annealing temperature of the primer is the first step for genetic diversity analysis using molecular markers such as RAPD (Random Amplified Polymorphic DNA). This study aims to determine annealing temperature (Ta) of RAPD primers on Kampar Mungbean. Methods included total DNA extraction, electrophoresis, and annealing temperature optimization of four RAPD markers namely OPD-20, OPI-06, OPI-13, dan OPX-13. Optimization was conducted by reducing the Tm value (Time melting) of each primer with 3 (Tm-3) and 5 (Tm-5). The results showed that the optimization using OPD-20 and OPX-13 produced bands at Tm-3 and Tm-5. Meanwhile, optimization using OPI-06 and OPI-13 resulted in bands at Tm-3. The next step was to choose the exact Ta based on the clear and bright band. In conclusion, exact Ta for OPD-20, OPI-06, OPI-13, and OPX-13 were 36,1°C, 38,1°C, 35,4°C, and 32,5°C respectively.

scholarly journals Influence of Cu on the Improvement of Magnetic Properties and Structure of L10 FePt Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1097
Author(s):  
Luran Zhang ◽  
Xinchen Du ◽  
Hongjie Lu ◽  
Dandan Gao ◽  
Huan Liu ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Annealing Temperature ◽  
Reduction Method ◽  
Solid Phase ◽  
Average Particle Size ◽  
Fept Nanoparticles ◽  
Average Particle ◽  
Phase Reduction ◽  
Different Temperatures ◽  
One Step

L10 ordered FePt and FePtCu nanoparticles (NPs) with a good dispersion were successfully fabricated by a simple, green, one-step solid-phase reduction method. Fe (acac)3, Pt (acac)2, and CuO as the precursors were dispersed in NaCl and annealed at different temperatures with an H2-containing atmosphere. As the annealing temperature increased, the chemical order parameter (S), average particle size (D), coercivity (Hc), and saturation magnetization (Ms) of FePt and FePtCu NPs increased and the size distribution range of the particles became wider. The ordered degree, D, Hc, and Ms of FePt NPs were greatly improved by adding 5% Cu. The highest S, D, Hc, and Ms were obtained when FePtCu NPs annealed at 750 °C, which were 0.91, 4.87 nm, 12,200 Oe, and 23.38 emu/g, respectively. The structure and magnetic properties of FePt and FePtCu NPs at different annealing temperatures were investigated and the formation mechanism of FePt and FePtCu NPs were discussed in detail.

scholarly journals Optimizing the Properties of La0.8Sr0.2CrO3 Thin Films through Post-Annealing for High-Temperature Sensing

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1802
Author(s):  
Dan Liu ◽  
Peng Shi ◽  
Yantao Liu ◽  
Yijun Zhang ◽  
Bian Tian ◽  
...  
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Annealing Temperature ◽  
Rf Sputtering ◽  
Maximum Size ◽  
Cross Sectional ◽  
Post Annealing ◽  
Maximum Conductivity ◽  
Different Temperatures ◽  
Post Annealing Temperature

La0.8Sr0.2CrO3 (0.2LSCO) thin films were prepared via the RF sputtering method to fabricate thin-film thermocouples (TFTCs), and post-annealing processes were employed to optimize their properties to sense high temperatures. The XRD patterns of the 0.2LSCO thin films showed a pure phase, and their crystallinities increased with the post-annealing temperature from 800 °C to 1000 °C, while some impurity phases of Cr2O3 and SrCr2O7 were observed above 1000 °C. The surface images indicated that the grain size increased first and then decreased, and the maximum size was 0.71 μm at 1100 °C. The cross-sectional images showed that the thickness of the 0.2LSCO thin films decreased significantly above 1000 °C, which was mainly due to the evaporation of Sr2+ and Cr3+. At the same time, the maximum conductivity was achieved for the film annealed at 1000 °C, which was 6.25 × 10−2 S/cm. When the thin films post-annealed at different temperatures were coupled with Pt reference electrodes to form TFTCs, the trend of output voltage to first increase and then decrease was observed, and the maximum average Seebeck coefficient of 167.8 µV/°C was obtained for the 0.2LSCO thin film post-annealed at 1100 °C. Through post-annealing optimization, the best post-annealing temperature was 1000 °C, which made the 0.2LSCO thin film more stable to monitor the temperatures of turbine engines for a long period of time.

scholarly journals Effect of Mn Content and Solution Annealing Temperature on the Corrosion Resistance of Stainless Steel Alloys

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ihsan-ul-Haq Toor
Keyword(s):  
Corrosion Resistance ◽  
Annealing Temperature ◽  
Electrochemical Impedance ◽  
Austenitic Stainless Steels ◽  
Electrochemical Tests ◽  
Linear Polarization Resistance ◽  
Ni Content ◽  
Different Temperatures ◽  
Acidic Chloride ◽  
Mn Content

The corrosion behavior of two specially designed austenitic stainless steels (SSs) having different Nickel (Ni) and Manganese (Mn) contents was investigated. Prior to electrochemical tests, SS alloys were solution-annealed at two different temperatures, that is, at 1030°C for 2 h and 1050°C for 0.5 h. Potentiodynamic polarization (PD) tests were carried out in chloride and acidic chloride, whereas linear polarization resistance (LPR) and electrochemical impedance spectroscopy (EIS) was performed in 0.5 M NaCl solution at room temperature. SEM/EDS investigations were carried out to study the microstructure and types of inclusions present in these alloys. Experimental results suggested that the alloy with highest Ni content and annealed at 1050°C/0.5 hr has the highest corrosion resistance.

scholarly journals In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR

2021 ◽  
Author(s):  
Fee Zimmermann ◽  
Maria Urban ◽  
Christian Krueger ◽  
Mathias Walter ◽  
Roman Woelfel ◽  
...  
Keyword(s):  
Binding Sites ◽  
Detrimental Effect ◽  
In Vitro Evaluation ◽  
In Vitro Transcripts ◽  
Nucleotide Mismatch ◽  
Reverse Primer ◽  
Forward Primer

A number of RT-qPCR assays for the detection of SARS-CoV-2 have been published and are listed by the WHO as recommended assays. Furthermore, numerous commercial assays with undisclosed primer and probe sequences are on the market. As the SARS-CoV-2 pandemic progresses, the virus accrues mutations, which in some cases - as seen with the B.1.1.7 variant - can outperform and push back other strains of SARS-CoV-2. If mutations occur in primer or probe binding sites, this can impact RT-qPCR results and impede SARS-CoV-2 diagnostics. Here we tested the effect of primer mismatches on RT-qPCR performance in vitro using synthetic mismatch in vitro transcripts. The effects of the mismatches ranged from a shift in ct values from -0.13 to +7.61. Crucially, we found that a mismatch in the forward primer has a more detrimental effect for PCR performance than a mismatch in the reverse primer. Furthermore, we compared the performance of the original Charite RdRP primer set, which has several ambiguities, with a primer version without ambiguities and found that without ambiguities the ct values are ca. 3 ct lower. Finally, we investigated the shift in ct values observed with the Seegene Allplex kit with the B.1.1.7 SARS-CoV-2 variant and found a three-nucleotide mismatch in the forward primer of the N target.

scholarly journals Filling gaps in the DNA barcode library - Aquatic insects (Ephemeroptera, Plecoptera, Trichoptera) from semi-mountainous and mountainous rivers of Ecoregion 6 (North Macedonia)

2021 ◽  
Vol 4 ◽  
Author(s):  
Biljana Rimcheska ◽  
Yanka Vidinova
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Aquatic Insects ◽  
Molecular Genetic ◽  
Dna Barcode ◽  
Balkan Peninsula ◽  
Molecular Genetic Data ◽  
Medium Quality ◽  
Total Dna ◽  
Data Library

This research provides pivotal molecular genetic data on the community structure of aquatic insects from semi-mountainous and mountainous rivers from the 6th Ecoregion that belongs to the territory of North Macedonia. The aim of this research is to fill the gaps for barcoding the aquatic macroinvertebrates from the Balkan Peninsula and check if the existing barcode library could provide improved identifications for the specimens that were not taxonomically determined to the lowest level possible. We analyzed 95 specimens from which total DNA was extracted and the COI barcode region amplified and sequenced. The taxa were selected from 20 different localities of the territory of western part of North Macedonia. The selected specimens were not determined to species-level in order to test the efficiency of the DNA barcoding methodology and what is missing in the DNA barcoding data library. From the result from one plate (95 specimens) we obtained: 16 samples without barodes, or failed and 10 samples did not have a match in the BOLD database. In the remining 69 samples, three were misidentified. In the total of 69 barcoded species new for the fauna of North Macedonia, 11 are mayflies: Baetis melanonyx, Ecdyonurus vitoshensis, E. macani; stonefly Isoperla vjose; and caddisflies: Agapetus delicatulus, Athripsodes bilineatus, Glossosoma klotho, Lepidostoma basale, Helicopsyche bacescui, Tinodes unicolor and Odontocerum hellenicum. We have also four rarely found species: Zwicknia bifrons, Drussus tenellus, Hydropsyche botosaneanui and Hydropsyche bulbifera, and one species without barcode available as Ecdyonurus sp. SK2 (potential new species). We found 83% efficiency of DNA barcoding, where some samples failed or were with low or medium quality for some specimens, as for the representatives from the genera Baetis, Oxietyra and Rhyacophila. In conclusion we can confirm that 10 of the selected vouchers need to be further identified by morphology and to be added in the BOLD barcode library, and maybe we'll have the possibility to describe a new species as well.

scholarly journals Photocatalytic Activity and Optical Properties of Blue Persistent Phosphors under UV and Solar Irradiation

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
C. R. García ◽  
L. A. Diaz-Torres ◽  
J. Oliva ◽  
M. T. Romero ◽  
P. Salas
Keyword(s):  
Photocatalytic Activity ◽  
Annealing Temperature ◽  
Uv Light ◽  
Solar Irradiation ◽  
Photoluminescence Spectra ◽  
X Ray Diffraction ◽  
X Ray ◽  
Water Cleaning ◽  
Different Temperatures ◽  
The Eu

Blue phosphorescent strontium aluminosilicate powders were prepared by combustion synthesis route and a postannealing treatments at different temperatures. X-ray diffraction analysis showed that phosphors are composed of two main hexagonal phases: SrAl2O4and Sr3Al32O51. The morphology of the phosphors changed from micrograins (1000°C) to a mixture of bars and hexagons (1200°C) and finally to only hexagons (1300°C) as the annealing temperature is increased. Photoluminescence spectra showed a strong blue-green phosphorescent emission centered atλem=455 nm, which is associated with4f65d1→4f6  (8S7/2)transition of the Eu2+. The sample annealed at 1200°C presents the highest luminance value (40 Cd/m2) with CIE coordinates (0.1589, 0.1972). Also, the photocatalytic degradation of methylene blue (MB) under UV light (at 365 nm) was monitored. Samples annealed at 1000°C and 1300°C presented the highest percentage of degradation (32% and 38.5%, resp.) after 360 min. In the case of photocatalytic activity under solar irradiation, the samples annealed at 1000°C, 1150°C, and 1200°C produced total degradation of MB after only 300 min. Hence, the results obtained with solar photocatalysis suggest that our powders could be useful for water cleaning in water treatment plants.

Ajustement par recuits rapides RTA (rapid thermal annealing) des contraintes dans des couches minces de tungstène utilisées comme absorbant pour des masques à rayons X

1991 ◽  
Vol 69 (3-4) ◽  
pp. 451-455 ◽  
Author(s):  
H. Lafontaine ◽  
J. F. Currie ◽  
S. Boily ◽  
M. Chaker ◽  
H. Pépin
Keyword(s):  
Thermal Annealing ◽  
Rapid Thermal Annealing ◽  
Annealing Temperature ◽  
Rf Power ◽  
X Ray ◽  
Stress Changes ◽  
Different Temperatures ◽  
Absorbing Layer ◽  
Good Agreement ◽  
Sputtering System

Tungsten thin films are deposited with a triode sputtering system in order to obtain an absorbing layer for X-ray masks. The mechanical stress is studied as a function of different pressure and RF power conditions during deposition. Rapid thermal annealing at different temperatures and durations is performed in order to produce films under low compressive stress. We observe that the stress changes occur over the time scale of seconds at the annealing temperature and that the corresponding activation energies are low (60 meV). Grain growth in a preferred orientation explains the observed changes in stress. The magnitude in the change of stress is in good agreement with a model proposed by Hoffman et al. relating the stress to grain size and grain boundary dimensions. [Journal translation]

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