scholarly journals Antioxidative Activity of Tithonia Diversifolia Extract in Streptozotocin-Induced Diabetic Rats.

2021 ◽  
Vol 913 (1) ◽  
pp. 012087
Author(s):  
R Solfaine ◽  
I S Hamid ◽  
L Muniroh
Keyword(s):  
Blood Glucose ◽  
Antioxidative Activity ◽  
Sandwich Elisa ◽  
Positive Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Tithonia Diversifolia ◽  
High Blood Glucose ◽  
Male Wistar Rats ◽  
Herbal Plant

Abstract Diabetes mellitus (DM) is metabolic disease characterized by high blood glucose. Tithonia diversifolia (TD) is a traditional herbal plant that contains anti-oxidative substances to reduce toxicity by free radical molecules. This study is aimed to analyzed effect of Tithonia diversifolia extract on diabetic rats. Dried leaves of Tithonia diversifolia plant (Balittro,Bogor) were sieved and macerated using 96% ethanol. TD leaves extract was dissolved in 0.1% sodium carboxymethyl cellulose (CMC-Na). Twenty-four male Wistar rats (Rattus norvegicus) were allocated into four groups; The control received normal saline (P0). The positive control received 0.1% CMC-Na (P1), the treatment received 100 mg/kg bw of TD extracts (P2) and cathecin 10 mg/kg bw (P3) respectively for 7 days. Bloods were collected for analysis of blood glucose (BG) and alkaline phospathase (ALP). The levels of MDA and SOD concentrations were conducted by Sandwich-ELISA. Based on result showed that feeding TD extract significantly could decrease the level of BG and ALP concentration compared to the positive control group (p<0.05). Level of MDA was decreased meanwhile level of SOD concentration significantly (p<0.05) on treatment group. It was concluded that administration of TD extract could restore normal blood glucose by antioxidant effect on diabetic rats.

scholarly journals Extract Ethanol of Poguntano in Alloxan Induced Diabetic Rats

2018 ◽  
Vol 17 (2) ◽  
pp. 251-254
Author(s):  
Sry Suryani Widjaja ◽  
Rusdiana
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medical Science ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
High Blood Glucose ◽  
Endothelin 1 ◽  
Back Ground ◽  
Male Wistar Rats

Back Ground: Diabetes mellitus is a group of metabolic disease with high blood glucose level above 150 mg/dL over a prolonged period. The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances that lead to micro and macrovascular complications. Poguntano (Picria fel-terrae Merr) from family Scrophulariaceae found in most part of Indonesia, has been used as traditional plant for treatment of diabetes.Objective: The purpose of this study was to investigate the effect of extract ethanol of Poguntano in alloxan induced diabetic rats.Method: Fifteen male Wistar rats with body weight of 150-200 gr were given intra peritoneal injection of 150 mg/kg Alloxan to induce diabetes. These were divided into three groups (control diabetic, group given insulin and group given Extract ethanol of Poguntano 200 mg/Kg body weight) and one control normal control. The duration of study was 4 weeks; blood glucose and Endothelin-1 were measured for all groups.Results : Extract ethanol of Poguntano 200 mg showed significant results (p<0.001) in lowering blood glucose in Alloxan induced diabetic rats compared to control diabetic, but did not show superior to insulin group (p=0.892). Endothelin-1 showed statistical significant between group with normal rats and diabetic control rats. (p=0.009) but did not in the other groups. Levels of Endothelin-1 was higher in the diabetic control group with the median 1.95 (1,78- 3.1).Conclusion: In our study we found that extract ethanol of Poguntano showed significant hypoglycemic activity and lowering the Endothelin-1 level in alloxan induced diabetic rats.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.251-254

scholarly journals Inhibition ofα-Glucosidase by Thiosulfinate as a Target for Glucose Modulation in Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Abdulrahman L. Al-Malki
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Hydrolytic Enzyme ◽  
Positive Control ◽  
Diabetic Rats ◽  
Postprandial Hyperglycemia ◽  
Postprandial Blood Glucose ◽  
Control Group ◽  
Albino Rats ◽  
Predisposing Factor

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage.α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativumL.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inhibitor of intestinalα-glucosidase, to reduce postprandial blood glucose. Male albino rats were randomly assigned to five different groups (n=10/group). Group 1 served as the control group. Groups 2–5 were injected intraperitoneally with a single dose of streptozotocin (STZ) to induce diabetes. Group 2 comprised untreated diabetic rats. Groups 3 and 4 contained diabetic rats that were given THIO orally (20 mg/kg body weight/day and 40 mg/kg body weight/day, resp.). Group 5 was the positive control having diabetic rats treated orally with acarbose (10 mg/kg body weight/day; positive control). Diabetic rats treated with THIO displayed a significant blood glucose reduction (p<0.001and < 0.01 by analysis of variance, resp.) and a significant elevation in insulin compared with that of untreated rats. THIO is an effective noncompetitive intestinalα-glucosidase inhibitor that promotes hypoglycemic action (p<0.001) in STZ-injected rats. THIO is a promising agent for the management of postprandial hyperglycemia.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals Effects of goat milk kefir fortified with vitamin D3 on Interleukin-18 levels in diabetic rats

Food Research ◽  
2020 ◽  
Vol 4 (S3) ◽  
pp. 197-201
Author(s):  
K.D. Anggraeni ◽  
G. Anjani ◽  
M. Ardiaria ◽  
C. Nissa ◽  
S.Y. Huang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Vitamin D3 ◽  
Fasting Blood Glucose ◽  
Goat Milk ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Group Design

Hyperglycemia causes increased oxidative stress through an imbalance of reactive oxygen species and antioxidative mechanisms. It stimulates the production of inflammatory mediators and cytokines such as TNF-α, interleukin (IL)-1, and IL-18. Goat milk kefir and vitamin D3 have potential as antioxidants and anti-inflammatory agents that can repair damage to pancreatic β cells. This study analyzed the effects of goat milk kefir fortified with vitamin D3 on the IL-18 level in diabetic rats. An experimental randomized pre-post test with control group design was conducted on 20 male Wistar rats divided into four groups, namely negative control (K-), positive control (K+), treatment with unfortified kefir (P1), and treatment with kefir fortified with vitamin D3. The intervention lasted 34 days. Fasting blood glucose and IL-18 levels were measured before and after intervention. Blood glucose and IL-18 levels were analyzed using the glucose oxidase p-aminophenol method and enzyme-linked immunosorbent assay, respectively. No significant increase in the IL-18 level was found in the P1 group with a median of 56.5 (10–252.7) pg/mL to 148.2 (106.8–428.3) pg/mL (p = 0.465) or P2 group with a median of 117.3 (91.8–146.8) pg/mL to 246.7 (168.8–311) pg/mL (p = 0.068), and no significant increase was observed in blood glucose levels in the P1 group (366.9±134.8 mg/dL to 462.1±156.9 mg/dL, p = 0.357) or P2 group (415.0±203.8 mg/dL to 258±129 mg/dL, p = 0.463). Goat milk kefir fortified with vitamin D3 could maintain blood glucose and IL-18 levels.

scholarly journals Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

2018 ◽  
Vol 197 ◽  
pp. 07007
Author(s):  
Erza Genatrika ◽  
Visda Nila Happy Laksari ◽  
Tjiptasurasa Tjiptasurasa
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diabetic Rats ◽  
Musa Acuminata ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

scholarly journals RESPON AKTIVITAS TIKUS WISTAR JANTAN AKIBAT KONDISI DIET TINGGI SUKROSA DIUKUR MENGGUNAKAN PEREKAM AKTIVITAS

Jurnal BIOMA ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 104
Author(s):  
Yanuar Restu Wijaya ◽  
Koekoeh Santoso ◽  
Isdoni Isdoni ◽  
Atin Supiyani
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Feeding Activity ◽  
Control Group ◽  
High Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Track System ◽  
Male Wistar Rats ◽  
Auto Track

Excessive energi consumption play a role in increasing blood glucose levels (hyperglycemia), due to the inability of the hormone insulin to compensate the high blood glucose levels. Blood glucose receptor found in the hypothalamus of VMH (Ventro Medial Hypothalamus) and LH (the Lateral Hypothalamus) is able to detect and change the settings for the feeding activity. The aim of this study was to obtain the relationship between high sucrose feeding on male Wistar rats of behavior and activity. This method uses Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% sucrose, the second group was given additional feed 40% sucrose, a third group was given additional feed 60% sucrose, and the last as a control group. This feed given continuously for 70 days.Observation of activities conducted using Opto-varimex® auto-track system ver.4.31. The results showed an corellation between activity based doses of sucrose.

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

scholarly journals Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats

2021 ◽  
Vol 5 (2) ◽  
pp. 17
Author(s):  
Sajad Nikkhah ◽  
Rahman Jafari Hafshejan ◽  
Farshid Gheibi Hajivar ◽  
Khalil Khashei ◽  
Sara Afzali
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Changes ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Since the liver is among the primary organs susceptible to the effects of hyperglycaemia, diabetes mellitus (DM) could be a risk factor for the development and progression of liver damage. In present study, since no side-effects from the herbal medicine have been reported, the effect of silymarin on blood glucose concentration, hepatic histopathological changes and FOXA2 and FOXA3 gene expression, which are key genes in liver regeneration, was investigated. In this fundamental with experimental approach study, 40 male Wistar rats weighing 180-220 g were used. Rats were kept under the standard conditions of temperature of 20-22°C and humidity of 50% and consecutive 12-hour periods of light and darkness. Rats were randomly divided into five different groups (n=8 each), including healthy control rats, diabetic control rats, diabetic rats receiving silymarin (50, 100 and 150 mg/kg). Diabetes was induced by injecting streptozotocin (50 mg/kg B.W., i.p.). For 4 weeks silymarin groups received the drug once every three days through gavage and fasting blood glucose concentration measured once every 10 days. At the end of a month experiment, livers were harvested for hepatic histopathological and FOXA2 and FOXA3 gene expression changes analysis. In the diabetic rats treated with silymarin (50, 100 and 150 mg/kg), by comparison with the diabetic control group (p<0.05), glucose levels decreased significantly. Moreover, FOXA2 and FOXA3 expression in diabetic groups treated with silymarin significantly increased compared to diabetic control group (p<0.05). Hepatic histopathological changes were improved in the treated groups.The present study indicates that silymarin significantly decreased blood glucose concentration and increased the FOXA2 and FOXA3 gene products level. Hence, silymarin is able to improve some of the symptoms associated with diabetes and possesses hepatoprotective effects in streptozotocin-induced diabetic rats.

scholarly journals Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

2017 ◽  
Vol 9 (3) ◽  
pp. 312-317
Author(s):  
KARTIAWATI ALIPIN ◽  
EMA PURNAMA SARI ◽  
MADIHAH MADIHAH ◽  
TIA SETIAWATI ◽  
NINING RATNINGSIH ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Kidney Damage ◽  
Diabetic Rat ◽  
Control Group ◽  
Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level ≥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

The Effect of Herbal Infusion on Fasting Blood Glucose (FBS) and Malondyaldehyde (MDA) Levels in Diabetic Rats

2020 ◽  
Vol 1009 ◽  
pp. 9-14
Author(s):  
Apriliani Ismi Fauziah ◽  
Lintang Dian Saraswati ◽  
Ari Udiyono ◽  
Beti Safitri
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Aloe Vera ◽  
Control Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats

Aloe vera, ginger, and sappan wood have recently gained attention as a potent antioxidant. This study was undertaken to analyze the possible effects of oral administration of herbal infusion (the combination of Aloe vera, ginger, and sapang wood) on fasting blood glucose (FBS) and malondialdehyde (MDA) levels in diabetic rats. Forty male albino rats (150-250 gm) were divided into five groups (n=8 in each group): alloxan-induced diabetic (negative control group/K-), acarbose treated (positive control group/K+), diabetic rats treated with herbal infusion dose 6.75 mg/150 gmbw (P1), diabetic rats treated with herbal infusion dose 13.5 mg/150 gmbw (P2), and diabetic rats treated with herbal infusion dose 20.25 mg/150 gmbw (P3). Male albino rats induced with a dose of alloxan 120 mg/kgbw and also 5% glucose after 6 hours of alloxan induction. FBS and MDA levels of each rat were measured before induction of alloxan was performed as a control sample. After seven days and 14 days of herbal infusion administration, FBS parameters was investigated with a glucometer. In the last week, the concentration of MDA in serum blood was determined using a UV-VIS spectrophotometer with a wavelength of 545 nm. Diabetic rats exhibited a significant decrease in FBS and MDA. Administration of herbal infusion was reduced FBS significantly between groups (ANOVA, Bonferroni, p<0.05). The concentration of MDA in serum blood was decreased significantly on K-, K+, P1, P2, P3 compared to the control group (ANOVA, LSD, P<0.05). It could be postulated herbal infusion has anti-hyperglycemic and anti-diabetic effects of FBS and MDA in male albino rats Wistar strain.

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