In vitro study of Cratoxylum glaucumStem ethyl acetate extract as antidiabetic

Abstract Diabetes mellitus (DM) is a disease characterized by hyperglycemia due to decreased insulin secretion, or decreased insulin sensitivity, or both. If insulin is not available or the amount is low, then glucose cannot enter the cells and will continue to be in the bloodstream. DM can cause chronic complications such as eye complications, skin infections and stroke. DM treatment takes a long time by using synthetic drugs that will cause side effects, therefore the search for herbal-based drugs is the community’s choice. Idat plant (Cratoxylum glaucum) is a local plant from Bangka Belitung. Several studies on the genus Cratoxylum from China and Thailand showed a very high inhibitory ability against the inhibition of a-glucosidase enzymes, protein tyrosine phosphate, and carbohydrate hydrolysis enzymes because they contain phenolic compounds anthraquinones and xanthones. Therefore, the importance of more intensive research on C. glaucum species regarding the content of secondary metabolites in C. glaucum stems and their antidiabetic power. The extraction method in this research is maceration with ethyl acetate solvent. Qualitative examination of phenolic and flavonoid content using reagents, as well as antidiabetic testing was carried out in vitro with dengan α-glucosidase enzyme inhibition method. Based on qualitative testing of phenolic and flavonoid compounds, the content of these compounds was obtained. antidiabetic test showed that the-glucosidase inhibitor of ethyl acetate extract had very strong activity with an IC50 of 4.21 g/mL. Therefore, the stem of the idat plant can be used as a therapy for the treatment of diabetes mellitus.

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Antimicrobial Activity of Few Medicinal Plants against Clinically Isolated Human Cariogenic Pathogens—An In Vitro Study

ISRN Dentistry ◽

10.5402/2011/541421 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

H. Shyla Jebashree ◽

S. Jayasurya Kingsley ◽

Emmanuel S. Sathish ◽

D. Devapriya

Keyword(s):

Ethyl Acetate ◽

Inhibitory Concentration ◽

Ethyl Acetate Extract ◽

In Vitro Study ◽

Ethanol Extract ◽

Psidium Guajava ◽

Terminalia Chebula ◽

Zone Of Inhibition ◽

Mimusops Elengi

Hexane, ethyl acetate, ethanol and methanol extracts of Psidium guajava, Terminalia chebula, Mimusops elengi and Achyranthes aspera were tested against the dental caries causing bacteria Streptococcus mutans and fungus Candida albicans isolated from caries infected patients. All the four extracts of P. guajava showed activity against both S. mutans and C. albicans. Maximum zone of inhibition was observed in ethyl acetate of P. guajava. The four extracts of T. chebula and M. elengi showed antibacterial activity against S. mutans. M. elengi extracts and ethanol extract of T. chebula did not show any antifungal activity against C. albicans. Except for the hexane extract of A. aspera, the other three extracts showed activity against the tested microbes. The ethyl acetate P. guajava leaf extract showed the minimum inhibitory concentration (MIC) against S. mutans to be <0.076 mg/mL in both MHB and BHI. The P. guajava ethyl acetate extract was subjected to GC-MS.

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In vitro antioxidant and antityrosinase activities of Manilkara kauki

Acta Pharmaceutica ◽

10.2478/acph-2021-0009 ◽

2021 ◽

Vol 71 (1) ◽

pp. 153-162 ◽

Cited By ~ 1

Author(s):

Sirinada Srisupap ◽

Chanya Chaicharoenpong

Keyword(s):

Ethyl Acetate ◽

Crude Extract ◽

Ethyl Acetate Extract ◽

Total Phenolic ◽

Tropical Plant ◽

Potential Source ◽

Antityrosinase Activity ◽

Phenolic And Flavonoid ◽

Different Parts

AbstractManilkara kauki L. Dubard is a tropical plant in the genus Manilkara of family Sapotaceae. This study investigated the total phenolic and flavonoid contents, and antioxidant and antityrosinase activities in different parts of M. kauki (fruits, leaves, seeds, stem barks and woods) and in fractions of stem barks. The total phenolic and flavonoid contents of the methanol and aqueous crude extracts of different parts of M. kauki ranged from 10.87 to 176.56 mg GAE (gallic acid equivalents) per gram of crude extract and 14.33 to 821.67 mg QE (quercetin equivalents) per gram of crude extract, resp. Leaves and stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and anti-tyrosinase activities than fruits, seeds and woods. Stem barks were sequentially extracted with n-hexane, ethyl acetate, methanol and water and then the fractionated extracts were subjected to antioxidant and antityrosinase activities testing. The ethyl acetate and methanol extracts of stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and antityrosinase activities than the n-hexane and aqueous extracts. Moreover, ethyl acetate extract of M. kauki stem exhibited the highest antityrosinase activity. It may be a potential source of tyrosinase inhibitors for pharmaceutical and cosmetic applications.

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Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

PeerJ ◽

10.7717/peerj.7022 ◽

2019 ◽

Vol 7 ◽

pp. e7022 ◽

Cited By ~ 4

Author(s):

James William ◽

Peter John ◽

Muhammad Waseem Mumtaz ◽

Ayoub Rashid Ch ◽

Ahmad Adnan ◽

...

Keyword(s):

Diabetes Mellitus ◽

Antioxidant Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Leaf Extracts ◽

Synthetic Drugs ◽

Promising Source ◽

Phenolic And Flavonoid ◽

Tof Ms

Background Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic H. lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out the first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

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The In Vitro α-Glucosidase Inhibition Activity of Various Solvent Fractions of Tamarix dioica and 1H-NMR Based Metabolite Identification and Molecular Docking Analysis

Plants ◽

10.3390/plants10061128 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1128

Author(s):

Aamir Niaz ◽

Ahmad Adnan ◽

Rashida Bashir ◽

Muhammad Waseem Mumtaz ◽

Syed Ali Raza ◽

...

Keyword(s):

Diabetes Mellitus ◽

Ethyl Acetate ◽

1H Nmr ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Docking Analysis ◽

Dried Extract ◽

Phenolic And Flavonoid ◽

Molecular Docking Analysis

The Tamarix dioica (T. dioica) is widely used medicinal plant to cure many chronic ailments. T. dioica is being used to manage diabetes mellitus in traditional medicinal system; however, very little scientific evidence is available on this plant in this context. The current study involves the fractionation of crude methanolic extract of T. dioica using n-hexane, ethyl acetate, chloroform, and n-butanol. The screening for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was carried out. The in vitro antidiabetic potential was assessed by measuring α-glucosidase inhibition. Total phenolic and flavonoid contents were also determined for each fraction. The metabolites were identified using highly sensitive and emerging 1H-NMR technique. The results revealed the ethyl acetate fraction as the most potent with DPPH scavenging activity of 84.44 ± 0.21% and α-glucosidase inhibition with IC50 value of 122.81 ± 2.05 µg/mL. The total phenolic and flavonoid content values of 205.45 ± 1.36 mg gallic acid equivalent per gram dried extract and 156.85 ± 1.33 mg quercetin equivalent per gram dried extract were obtained for ethyl acetate fraction. The bucketing of 1H-NMR spectra identified 22 metabolites including some pharmacologically important like tamarixetin, tamaridone, quercetin, rutin, apigenin, catechin, kaempferol, myricetin and isorhamnetin. Leucine, lysine, glutamic acid, aspartic acid, serine, and tyrosine were the major amino acids identified in ethyl acetate fraction. The molecular docking analysis provided significant information on the binding affinity among secondary metabolites and α-glucosidase. These metabolites were most probably responsible for the antioxidant activity and α-glucosidase inhibitory potential of ethyl acetate fraction. The study ascertained the ethnomedicinal use of T. dioica to manage diabetes mellitus and may be a helpful lead towards naturopathic mode for anti-hyperglycemia.

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In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

Current Drug Discovery Technologies ◽

10.2174/1570163817666200727101300 ◽

2020 ◽

Vol 17 ◽

Author(s):

Shubhaisi Das ◽

Sunanda Burman ◽

Goutam Chandra

Keyword(s):

Ethyl Acetate ◽

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Positive Control ◽

Leaf Extracts ◽

Ms Analysis ◽

Ft Ir ◽

Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

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α-glucosidase Inhibitory Activity of Extracts and Compounds from the Leaves of Ruellia tuberosa L.

The Natural Products Journal ◽

10.2174/2210315511666210218214955 ◽

2021 ◽

Vol 11 ◽

Author(s):

Le Nguyen Tuong Vi ◽

Nguyen Ngoc Tuan ◽

Quach Tong Hung ◽

Pham Thi Nhat Trinh ◽

Tong Thanh Danh ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Spectroscopic Method ◽

Positive Control ◽

Strong Activity ◽

Butanol Extract ◽

Glucosidase Inhibitor ◽

Inhibitory Activities ◽

Bio Assay ◽

Ethyl Acetate Extracts

Background: In recent years, the study of the structure and biological activity of medicinal plants has a particularly important to search for diabetes medicine. Ruellia tuberosa is used to treat various diseases such as diabetes by inhibiting the activity of α-glucosidase. Objective: In this study, experiment was designed to isolated isolate and identified identify α-glucosidase inhibitory extracts and compounds from Ruellia tuberosa L. through bio-assay guided isolation. Method: Dry powder of Ruellia tuberosa L. was extracted with 70% ethanol, then liquid-liquid partition with n-hexane, ethyl acetate and butanol, respectively. The extracts were evaluated with α-glucosidase inhibition. The potential extracts were isolated and identified compounds. The effects of these compounds on the α-glucosidase inhibitory were evaluated. Results: The a-glucosidase inhibitory activities showed that the n-hexane, ethyl acetate and the butanol extract had the α-glucosidase inhibition with an IC50 of 46.2 0.9, 6.6 2.9 and 8.9  μg/mL, respectively. From the n-hexane and ethyl acetate extracts, the structures of four compounds were elucidated by NMR spectroscopic method, including lupeol (1), syringaresinol (2), apigenin (3), verbascoside (4). The a-glucosidase inhibitory activities showed that all isolated compounds were more active than the positive control - acarbose with an IC50 of 37.5  0.4; 19.5  0.2; 20.1  0.3; 69.3  0.2 µg/mL, respectively. Conclusion: The ethyl acetate extract showed strong activity about 19 times more than positive control - acarbose. The compound syringaresinol (2) was the most powerful α-glucosidase inhibitor. Therefore, Ruellia tuberosa L. contains many compounds that can inhibit α-glucosidase activity.

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Chemical constituents, in vitro antioxidant and antimicrobial properties of ethyl acetate extract obtained from Cytisus triflorus l’Her

Natural Product Research ◽

10.1080/14786419.2018.1519816 ◽

2018 ◽

Vol 34 (11) ◽

pp. 1586-1590 ◽

Cited By ~ 2

Author(s):

Wassila Benabderrahmane ◽

Amel Amrani ◽

Ouahiba Benaissa ◽

Marta Lores ◽

J. Pablo Lamas ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

Antimicrobial Properties

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Machine Learning and In Vitro Chemical Screening of Potential α-Amylase and α-Glucosidase Inhibitors from Thai Indigenous Plants

Nutrients ◽

10.3390/nu14020267 ◽

2022 ◽

Vol 14 (2) ◽

pp. 267

Author(s):

Tarapong Srisongkram ◽

Sasisom Waithong ◽

Thaweesak Thitimetharoch ◽

Natthida Weerapreeyakul

Keyword(s):

Diabetes Mellitus ◽

Machine Learning ◽

Inhibitory Activity ◽

In Vitro Study ◽

Ethanolic Extract ◽

Accuracy Score ◽

Indigenous Plants ◽

Predisposing Factor ◽

Chemical Screening

Diabetes mellitus is a major predisposing factor for cardiovascular disease and mortality. α-Amylase and α-glucosidase enzymes are the rate-limiting steps for carbohydrate digestion. The inhibition of these two enzymes is clinically used for the treatment of diabetes mellitus. Here, in vitro study and machine learning models were employed for the chemical screening of inhibiting the activity of 31 plant samples on α-amylase and α-glucosidase enzymes. The results showed that the ethanolic twig extract of Pinus kesiya had the highest inhibitory activity against the α-amylase enzyme. The respective ethanolic extract of Croton oblongifolius stem, Parinari anamense twig, and Polyalthia evecta leaf showed high inhibitory activity against the α-glucosidase enzyme. The classification analysis revealed that the α-glucosidase inhibitory activity of Thai indigenous plants was more predictive based on phytochemical constituents, compared with the α-amylase inhibitory activity (1.00 versus 0.97 accuracy score). The correlation loading plot revealed that flavonoids and alkaloids contributed to the α-amylase inhibitory activity, while flavonoids, tannins, and reducing sugars contributed to the α-glucosidase inhibitory activity. In conclusion, the ethanolic extracts of P. kesiya, C. oblongifolius, P. anamense, and P. evecta have the potential for further chemical characterization and the development of anti-diabetic recipes.

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Effect of Eucalyptus globulus leaves extracts on in vitro rumen fermentation, methanogenesis, degradability and protozoa population

Annals of Animal Science ◽

10.2478/aoas-2018-0006 ◽

2018 ◽

Vol 18 (3) ◽

pp. 753-767 ◽

Cited By ~ 4

Author(s):

Amina Boussaada ◽

Rabah Arhab ◽

Serena Calabrò ◽

Raffaella Grazioli ◽

Maria Ferrara ◽

...

Keyword(s):

Organic Matter ◽

Ethyl Acetate ◽

Eucalyptus Globulus ◽

Ethyl Acetate Extract ◽

Rumen Fermentation ◽

Gas Production ◽

Aqueous Extracts ◽

Ch4 Production ◽

In Vitro Gas Production

Abstract The aim of the research was to evaluate the effect of three Eucalyptus globulus extracts rich in phenolic compounds, especially flavonoids, on rumen fermentation, methane (CH4) production, organic matter degradability and protozoa population using an in vitro gas production technique. Four concentrations (0, 50, 75 and 100 mg) of three Eucalyptus extracts (ethyl acetate, n-butanol and aqueous) were added to a diet of ruminants (forage: concentrate ratio 60:40) and incubated at 39°C under anaerobiosis with buffered rumen fluid. After 24 h, the fermentation fluid was analysed for ammonia-N and volatile fatty acids (VFA). Organic matter degradability (OMD) and protozoa were also determined; in vitro gas production was also recorded and CH4 concentration was measured. Compared to the control, CH4 production was significantly lower for ethyl acetate extract (P<0.05), but higher for n-butanol and aqueous extracts. Production of ammonia- N was lower in all Eucalyptus extracts (P<0.05). Propionate production (P<0.05) increased for ethyl acetate and n-butanol extracts, whereas no effect was registered for VFA, for all Eucalyptus extracts. Ethyl acetate extract decreased in vitro OMD (P<0.05), whereas n-butanol and aqueous extracts were comparable to the control. Protozoa population decreased (P<0.05) for all extracts in comparison with the control. Eucalyptus ethyl acetate extract might be promising to be used as a potent anti-methanogenic additive. Moreover, the assessment of the right dosage seems to be important to decrease methane production, without reducing feed nutritional value.

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Preliminary in vitro study of anti-oxidant activity and anti-diabetic potential of plant extracts from 4 herbal substances not traditionally used for treatment of diabetes mellitus

Pharmacia ◽

10.3897/pharmacia.68.e72769 ◽

2021 ◽

Vol 68 (4) ◽

pp. 755-762

Author(s):

Dora Trifonova ◽

Anna Gavrilova ◽

Galina Dyakova ◽

Genadi Gavrilov ◽

Maya Yotova ◽

...

Keyword(s):

Diabetes Mellitus ◽

Inhibitory Activity ◽

In Vitro Study ◽

Biologically Active ◽

Total Phenolic ◽

Water Extracts ◽

Sambucus Ebulus ◽

Anti Oxidant ◽

Treatment Of Diabetes

The focus of the presented study is the in vitro anti-oxidant activity and anti-diabetic potential of water extracts from the following four herbal substances, not traditionally used for treatment of diabetes mellitus – leaves of Sambucus ebulus L. and Prunus mahaleb L., and flowering stems of Cichorium intybus L. and Satureja kitaibelii Wierzb. ex Heuff. The water extracts are obtained through ultrasonication. The extract of S. kitaibelii stands out due to its highest values in all studied indicators – total phenolic content, scavenging potential (DPPH, ABTS) and α-glucosidase inhibitory activity which was six times higher than acarbose. The extract of C. intybus also showed significant α-glucosidase inhibitory activity compared to acarbose. The flowering stems of both species are promising sources of biologically active substances for blood sugar control in diabetes mellitus.

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