Water Extract of Artemisia annua L. Exhibits Hepatoprotective Effects Through Improvement of Lipid Accumulation and Oxidative Stress-Induced Cytotoxicity

The curry leaf water extract, which is rich in gallic acid, reversed paracetamol-induced liver damage in mice by reducing inflammation and oxidative stress.

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Kaempferol and Kaempferide Attenuate Oleic Acid-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22168847 ◽

2021 ◽

Vol 22 (16) ◽

pp. 8847

Author(s):

Fangfang Tie ◽

Jin Ding ◽

Na Hu ◽

Qi Dong ◽

Zhi Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Oleic Acid ◽

Lipid Metabolism ◽

Western Blot ◽

Blot Analysis ◽

Lipid Accumulation ◽

Hepg2 Cells ◽

Western Blot Analysis ◽

Heme Oxygenase 1 ◽

And Oxidative Stress

Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases which lacks ideal treatment options. Kaempferol and kaempferide, two natural flavonol compounds isolated from Hippophae rhamnoides L., were reported to exhibit a strong regulatory effect on lipid metabolism, for which the mechanism is largely unknown. In the present study, we investigated the effects of kaempferol and kaempferide on oleic acid (OA)-treated HepG2 cells, a widely used in vitro model of NAFLD. The results indicated an increased accumulation of lipid droplets and triacylglycerol (TG) by OA, which was attenuated by kaempferol and kaempferide (5, 10 and 20 μM). Western blot analysis demonstrated that kaempferol and kaempferide reduced expression of lipogenesis-related proteins, including sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD-1). Expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer binding proteins β (C/EBPβ), two adipogenic transcription factors, was also decreased by kaempferol and kaempferide treatment. In addition, western blot analysis also demonstrated that kaempferol and kaempferide reduced expression of heme oxygenase-1 (HO-1) and nuclear transcription factor-erythroid 2-related factor 2 (Nrf2). Molecular docking was performed to identify the direct molecular targets of kaempferol and kaempferide, and their binding to SCD-1, a critical regulator in lipid metabolism, was revealed. Taken together, our findings demonstrate that kaempferol and kaempferide could attenuate OA-induced lipid accumulation and oxidative stress in HepG2 cells, which might benefit the treatment of NAFLD.

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New Perspectives of S-Adenosylmethionine (SAMe) Applications to Attenuate Fatty Acid-Induced Steatosis and Oxidative Stress in Hepatic and Endothelial Cells

Molecules ◽

10.3390/molecules25184237 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4237 ◽

Cited By ~ 1

Author(s):

Laura Vergani ◽

Francesca Baldini ◽

Mohamad Khalil ◽

Adriana Voci ◽

Pietro Putignano ◽

...

Keyword(s):

Oxidative Stress ◽

Endothelial Cells ◽

Fatty Acid ◽

Lipid Accumulation ◽

Endothelium Dysfunction ◽

Beneficial Effects ◽

Mitochondrial Fatty Acid ◽

Oxidant Production ◽

Pleiotropic Functions ◽

And Oxidative Stress

S-adenosylmethionine (SAMe) is an endogenous methyl donor derived from ATP and methionine that has pleiotropic functions. Most SAMe is synthetized and consumed in the liver, where it acts as the main methylating agent and in protection against the free radical toxicity. Previous studies have shown that the administration of SAMe as a supernutrient exerted many beneficial effects in various tissues, mainly in the liver. In the present study, we aimed to clarify the direct effects of SAMe on fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells. Hepatoma FaO cells and endothelial HECV cells exposed to a mixture of oleate/palmitate are reliable models for hepatic steatosis and endothelium dysfunction, respectively. Our findings indicate that SAMe was able to significantly ameliorate lipid accumulation and oxidative stress in hepatic cells, mainly through promoting mitochondrial fatty acid entry for β-oxidation and external triglyceride release. SAMe also reverted both lipid accumulation and oxidant production (i.e., ROS and NO) in endothelial cells. In conclusion, these outcomes suggest promising beneficial applications of SAMe as a nutraceutical for metabolic disorders occurring in fatty liver and endothelium dysfunction.

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Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in Aβ-Exposed Hippocampal Neurons

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/7023091 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Nora E. Gray ◽

Jonathan A. Zweig ◽

Donald G. Matthews ◽

Maya Caruso ◽

Joseph F. Quinn ◽

...

Keyword(s):

Oxidative Stress ◽

Mitochondrial Dysfunction ◽

Mitochondrial Function ◽

Hippocampal Neurons ◽

Neuroblastoma Cells ◽

Water Extract ◽

Centella Asiatica ◽

Antioxidant Response ◽

And Oxidative Stress

Centella asiatica has been used for centuries to enhance memory. We have previously shown that a water extract of Centella asiatica (CAW) protects against the deleterious effects of amyloid-β (Aβ) in neuroblastoma cells and attenuates Aβ-induced cognitive deficits in mice. Yet, the neuroprotective mechanism of CAW has yet to be thoroughly explored in neurons from these animals. This study investigates the effects of CAW on neuronal metabolism and oxidative stress in isolated Aβ-expressing neurons. Hippocampal neurons from amyloid precursor protein overexpressing Tg2576 mice and wild-type (WT) littermates were treated with CAW. In both genotypes, CAW increased the expression of antioxidant response genes which attenuated the Aβ-induced elevations in reactive oxygen species (ROS) and lipid peroxidation in Tg2576 neurons. CAW also improved mitochondrial function in both genotypes and increased the expression of electron transport chain enzymes and mitochondrial labeling, suggesting an increase in mitochondrial content. These data show that CAW protects against mitochondrial dysfunction and oxidative stress in Aβ-exposed hippocampal neurons which could contribute to the beneficial effects of the extract observed in vivo. Since CAW also improved mitochondrial function in the absence of Aβ, these results suggest a broader utility for other conditions where neuronal mitochondrial dysfunction occurs.

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Aucubin inhibited lipid accumulation and oxidative stress via Nrf2/ HO ‐1 and AMPK signalling pathways

Journal of Cellular and Molecular Medicine ◽

10.1111/jcmm.14293 ◽

2019 ◽

Vol 23 (6) ◽

pp. 4063-4075 ◽

Cited By ~ 16

Author(s):

Bingyu Shen ◽

Chenxu Zhao ◽

Yue Wang ◽

Yi Peng ◽

Jiaqi Cheng ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Accumulation ◽

Signalling Pathways ◽

And Oxidative Stress

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Silibinin ameliorates hepatic lipid accumulation and oxidative stress in mice with non-alcoholic steatohepatitis by regulating CFLAR-JNK pathway

Acta Pharmaceutica Sinica B ◽

10.1016/j.apsb.2019.02.006 ◽

2019 ◽

Vol 9 (4) ◽

pp. 745-757 ◽

Cited By ~ 19

Author(s):

Yayun Liu ◽

Wei Xu ◽

Ting Zhai ◽

Jiaojiao You ◽

Yong Chen

Keyword(s):

Oxidative Stress ◽

Lipid Accumulation ◽

Jnk Pathway ◽

Hepatic Lipid ◽

Hepatic Lipid Accumulation ◽

Alcoholic Steatohepatitis ◽

And Oxidative Stress

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Önemli Tıbbi Bitkilerden Biri Olan Achillea millefolium L. (Civanperçemi) Bitkisinin Besin Elementi Konsantrasyonları ve Antioksidan Aktivitesi

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i3.590-594.4088 ◽

2021 ◽

Vol 9 (3) ◽

pp. 590-594

Author(s):

Handan Saraç ◽

Hasan Durukan ◽

Ahmet Demirbaş

Keyword(s):

Oxidative Stress ◽

Water Extract ◽

Total Antioxidant Status ◽

Stress Index ◽

Achillea Millefolium ◽

Nutrient Concentrations ◽

Oxidative Stress Index ◽

Total Antioxidant ◽

Oxidant Status ◽

And Oxidative Stress

In this study, it was aimed to determine the nutrient concentrations and antioxidant activity of Achillea millefolium L. (Yarrow), which is known to have medicinal properties. For this purpose, macro and micronutrient concentrations, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of water extract Yarrow plant, which is known to be collected from Divrigi district in Sivas province, were determined. The results have shown that the yarrow plant is sufficient in terms of some macro and micronutrient concentrations, and the nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium from macro elements, iron (Fe), zinc (Zn), manganese (Mn) and copper (Cu) from micro elements concentrations were determined as 1.01% N, 0.63% P, 2.43% K, 2.22% Ca, 0.70% Mg, 360.4 mg kg-1 Fe, 47.6 mg kg-1 Zn, 85.5 mg kg-1 Mn and 28.3 mg kg-1 Cu, respectively. It has been determined that its antioxidant potential is moderate. In addition, the plant’s oxidant capacity and oxidative stress index were found to be low.

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Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells

Antioxidants ◽

10.3390/antiox10081314 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1314

Author(s):

Seung-Cheol Lee ◽

Seung-Cheol Jee ◽

Min Kim ◽

Soee Kim ◽

Min Kyoung Shin ◽

...

Keyword(s):

Oxidative Stress ◽

Cytochrome P450 ◽

Cell Viability ◽

Lipid Accumulation ◽

Hepg2 Cells ◽

Nuclear Translocation ◽

The Body ◽

Ros Generation ◽

Related Factor ◽

And Oxidative Stress

Benzo[a]pyrene (B[a]P) is a potentially hepatotoxic group-1 carcinogen taken up by the body through ingestion of daily foods. B[a]P is widely known to cause DNA and protein damages, which are closely related to cell transformation. Accordingly, studies on natural bioactive compounds that attenuate such chemical-induced toxicities have significant impacts on public health. This study aimed to uncover the mechanism of curcumin, the major curcuminoid in turmeric (Curcuma longa), in modulating the lipid accumulation and oxidative stress mediated by B[a]P cytotoxicity in HepG2 cells. Curcumin treatment reduced the B[a]P-induced lipid accumulation and reactive oxygen spicies (ROS) upregulation and recovered the cell viability. Cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) and Cytochrome P450 subfamily B polypeptide 1 (CYP1B1) downregulation resulting from decreased aryl hydrocarbon receptor (AhR) translocation into nuclei attenuated the effects of B[a]P-induced lipid accumulation and repressed cell viability, respectively. Moreover, the curcumin-induced reduction in ROS generation decreased the nuclear translocation of Nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of phase-II detoxifying enzymes. These results indicate that curcumin suppresses B[a]P-induced lipid accumulation and ROS generation which can potentially induce nonalcoholic fatty liver disease (NAFLD) and can shed a light on the detoxifying effect of curcumin.

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