Aedes aegypti Immune Response and Its Potential Impact on Dengue Virus Transmission

The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.

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Experimental study of dengue virus infection in Aedes aegypti and Aedes albopictus: A comparative analysis on susceptibility, virus transmission and reproductive success

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2020.107445 ◽

2020 ◽

Vol 175 ◽

pp. 107445

Author(s):

Monika Soni ◽

Siraj A. Khan ◽

Chandra K. Bhattacharjee ◽

Prafulla Dutta

Keyword(s):

Experimental Study ◽

Comparative Analysis ◽

Reproductive Success ◽

Virus Infection ◽

Aedes Aegypti ◽

Dengue Virus ◽

Aedes Albopictus ◽

Virus Transmission ◽

Dengue Virus Infection

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Dengue and the introduction of mosquito-transmitted viruses into Australia

Microbiology Australia ◽

10.1071/ma16056 ◽

2016 ◽

Vol 37 (4) ◽

pp. 167

Author(s):

Andrew F van den Hurk

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Virus Transmission ◽

Current Situation ◽

Mosquito Vector ◽

And Control

Dengue virus outbreaks involving 100s of cases periodically occur in north Queensland, the area of Australia where the primary mosquito vector, Aedes aegypti, occurs. This article summarises the ecology, history, current situation and control of dengue virus transmission in Australia and examines the threat posed by newly emergent arboviruses, such as Zika and chikungunya viruses.

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Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission

International Journal of Molecular Sciences ◽

10.3390/ijms21186609 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6609

Author(s):

Alexander S. Gold ◽

Fabiana Feitosa-Suntheimer ◽

Ricardo V. Araujo ◽

Ryan M. Hekman ◽

Sultan Asad ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Extracellular Vesicles ◽

High Performance ◽

Dengue Infection ◽

Virus Transmission ◽

Viral Disease ◽

Extracellular Vesicle ◽

Transmission Cycle ◽

Packaging Strategy

Dengue is the most burdensome vector-borne viral disease in the world. Dengue virus (DENV), the etiological cause of dengue, is transmitted primarily by the Aedes aegypti mosquito. Like any arbovirus, the transmission cycle of dengue involves the complex interactions of a multitude of human and mosquito factors. One point during this transmission cycle that is rich in these interactions is the biting event by the mosquito, upon which its saliva is injected into the host. A number of components in mosquito saliva have been shown to play a pivotal role in the transmission of dengue, however one such component that is not as well characterized is extracellular vesicles. Here, using high-performance liquid chromatography in tandem with mass spectrometry, we show that dengue infection altered the protein cargo of Aedes aegypti extracellular vesicles, resulting in the packaging of proteins with infection-enhancing ability. Our results support the presence of an infection-dependent pro-viral protein packaging strategy that uses the differential packaging of pro-viral proteins in extracellular vesicles of Ae. aegypti saliva to promote transmission. These studies represent the first investigation into the function of Ae. aegypti extracellular vesicle cargo during dengue infection.

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Deteksi transmisi virus dengue pada nyamuk wild Aedes Aegypti betina di Kota Manado

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.14661 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Grace Trovancia ◽

Angle Sorisi ◽

Josef S.B. Tuda

Keyword(s):

Polymerase Chain Reaction ◽

Aedes Aegypti ◽

Dengue Virus ◽

Reverse Transcription ◽

Clinical Manifestations ◽

Virus Transmission ◽

Survey Study ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain

Abstract: Dengue hemorrhagic fever is an acute disease with clinical manifestations of hemorrhage caused by dengue virus infection. Manado is endemic dengue. Dengue virus has the ability to maintain its existence in nature through horizontal and vertical transmission. There are several ways to detect the dengue virus by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemistry Streptavidin Biotin Peroxidase Complex (ISBPC). This research aims to determine the wild Aedes aegypti population in Manado and to detect dengue virus in wild mosquito Aedes aegypti by ISBPC methods. This was a descriptive survey study with a cross sectional design to describe the transmission of dengue virus in wild mosquito Aedes aegypti in the city of Manado. The results showed that there were 5 wild Aedes aegypti mosquitoes positive for dengue virus, and 36 wild Aedes aegypti mosquitoes negative containing dengue virus. Conclusion: Of the 41 samples immunohistochemistry tested, 5 samples showed dengue virus transmission in wild mosquito Aedes aegypti in Manado which is a positive possibility of horizontal transmission.Keywords: detection of dengue virus, transmission, wild Aedes aegypti, Manado. Abstrak: Demam berdarah dengue adalah suatu penyakit akut dengan manifestasi klinis perdarahan yang disebabkan oleh infeksi virus dengue. Manado merupakan daerah endemis demam berdarah. Virus dengue memiliki kemampuan untuk mempertahankan keberadaannya di alam melalui transmisi horizontal dan vertikal. Ada beberapa cara untuk mendeteksi virus dengue yaitu Reverse Transcription-Polymerase Chain Reaction (RT-PCR) dan imunohistokimia Streptavidin Biotin Peroxidase Complex (SBPC). Penelitian ini bertujuan untuk mengetahui populasi nyamuk wild Aedes aegypti di Kota Manado dan mendeteksi virus dengue pada nyamuk wild Aedes aegypti dewasa menggunakan metode imunohistokimia streptavidin biotin peroxidase complex (ISBPC). Jenis penelitian ialah survei deskriptif dengan desain potong lintang untuk mengetahui gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado. Hasil pene;itian mendapatkan 5 nyamuk wild Aedes aegypti positif mengandung virus dengue, dan 36 nyamuk wild Aedes aegypti negatif mengandung virus dengue. Simpulan: Berdasarkan hasil penelitian dapat disimpulkan bahwa dari 41 sampel yang telah diuji imunohistokimia, 5 sampel gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado yang kemungkinan transmisi horizontal adalah positif. Kata kunci: deteksi virus dengue, transmisi, wild Aedes aegypti, Manado.

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