Adipose Collagen Fragment: A Novel Adipose-Derived Extracellular Matrix Concentrate for Skin Filling

2021 ◽  
Author(s):  
Yuchen Zhang ◽  
Xiangdong Zhang ◽  
Xiaoxuan Jin ◽  
Pan Zhang ◽  
Kaiyang Liu ◽  
...  
Keyword(s):  
Skin Aging ◽  
Therapeutic Effects ◽  
Dorsal Skin ◽  
Skin Rejuvenation ◽  
Collagen Remodeling ◽  
Patients Satisfaction ◽  
Dermal Thickness ◽  
Collagen Fragment ◽  
Phosphate Buffered Saline ◽  
Filling Capacity

Abstract Background Skin filler is an option for treatment of skin aging and wrinkle formation; however, currently used fillers are limited by poor biocompatibility, rapid degradation, and possible hypersensitivity reactions. However, autologous adipose tissue-derived products have been recognized as promising options for skin rejuvenation. Objectives This study aimed to develop a novel adipose-derived product for skin filling. Methods Adipose collagen fragment (ACF) was prepared through pulverization, filtration, and centrifugation. The macrography, structure, types of collagen, and cell viability of ACF were evaluated by immunostaining, Western blotting, and cell culture assays. ACF, nanofat and phosphate-buffered saline (9 spots/side, 0.01 ml/spot) were intradermally injected in the dorsal skin of 36 female BALB/c nude mice; then, the skin filling capacity and collagen remodeling process were investigated. Twenty-one female patients with fine rhytides in the infraorbital areas were enrolled and received ACF treatment as clinical applications. Therapeutic effects and patients’ satisfaction scores were recorded. Results The ACF yield from 50 ml of Coleman fat was 4.91 ± 0.25 ml. ACF contained nonviable cells and high levels of collagen I, collagen IV, and laminin. Fibroblasts and procollagen significantly increased in ACF and ACF-treated dermis (p < 0.05). 85.7% of patients were satisfied with the therapy results, and no infections, injection site nodules, or other unwanted side effects were observed. Conclusions ACF significantly improved dermal thickness and collagen synthesis and may serve as a potential autologous skin filler.

scholarly journals Polysaccharide Extracted fromLaminaria japonicaDelays Intrinsic Skin Aging in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Longyuan Hu ◽  
Jia Tan ◽  
Xiaomei Yang ◽  
Haitao Tan ◽  
Xiaozhen Xu ◽  
...  
Keyword(s):  
Skin Aging ◽  
Skin Tissue ◽  
Dorsal Skin ◽  
Young Control ◽  
Dermal Thickness ◽  
Skin Collagen ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Skin Samples ◽  
Aging Skin

This study aimed to determine the effect of topically appliedLaminariapolysaccharide (LP) on skin aging. We applied ointment containing LP (10, 25, and 50 μg/g) or vitamin E (10 μg/g) to the dorsal skin of aging mice for 12 months and young control mice for 4 weeks. Electron microscopy analysis of skin samples revealed that LP increased dermal thickness and skin collagen content. Tissue inhibitor of metalloprotease- (TIMP-) 1 expression was upregulated while that of matrix metalloproteinase- (MMP-) 1 was downregulated in skin tissue of LP-treated as compared to untreated aging mice. Additionally, phosphorylation of c-Jun N-terminal kinase (JNK) and p38 was higher in aging skin than in young skin, while LP treatment suppressed phospho-JNK expression. LP application also enhanced the expression of antioxidative enzymes in skin tissue, causing a decrease in malondialdehyde levels and increases in superoxide dismutase, catalase, and glutathione peroxidase levels relative to those in untreated aging mice. These results indicate that LP inhibits MMP-1 expression by preventing oxidative stress and JNK phosphorylation, thereby delaying skin collagen breakdown during aging.

scholarly journals Therapeutic Effects of Chinese Herbal Formula (PTQX) on NC/Nga Mice with Atopic Dermatitis-Like Skin Lesions

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Fenggen Yan ◽  
Jing Zhang ◽  
Xiong Li ◽  
Xiumei Mo ◽  
Junfeng Liu ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Lymph Nodes ◽  
Immunoglobulin E ◽  
Skin Lesions ◽  
Therapeutic Effects ◽  
Herbal Formula ◽  
Dorsal Skin ◽  
Chinese Herbal Formula ◽  
Chinese Herbal

Atopic dermatitis (AD), also known as atopic eczema, is a chronic pruritic inflammatory skin disease. The available systemic therapies for atopic dermatitis are inadequate. Objective. This study aimed to evaluate the effects of the Chinese herbal formula Pei Tu Qing Xin (PTQX) on dermatitis severity and ear swelling, immunomodulation, and the infiltration of mast cells in a mouse model of 1-chloro-2,4-dinitrobenzene- (DNCB-) induced AD. Methods. AD-like symptoms were induced by DNCB in NC/Nga mice. Skin lesions, dermatitis, ear swelling, and scratching behaviour were evaluated. Changes in the T-helper type 1 (Th1), Th2, Th17, and regulatory T (Treg) subtypes and immunoregulation in the spleen and lymph nodes were detected by flow cytometry. Results. Histopathological and immunohistochemical analyses demonstrated that PTQX decreased the DNCB-mediated induction of mast cells and infiltration of inflammatory cells in the ear and dorsal skin. PTQX also reduced the DNCB-induced increase in the serum immunoglobulin E level, pruritus, and dermatitis (red, flaky areas) on the dorsal skin. Furthermore, PTQX regulated the balance between the populations of Th1, Th2, Th17, and Treg cells (particularly the latter two) in the lymph nodes. Conclusions. Our results suggest that the Chinese herbal formula PTQX can alleviate symptoms of AD, such as epithelial damage, redness, swelling, and pruritus, and potentially be used to treat this condition.

scholarly journals Antioxidant, Anti-Inflammatory, and Anti-Aging Potential of a Kalmia angustifolia Extract and Identification of Some Major Compounds

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1373 ◽  
Author(s):  
Alexe Grenier ◽  
Jean Legault ◽  
André Pichette ◽  
Lorry Jean ◽  
Audrey Bélanger ◽  
...  
Keyword(s):  
Biological Activities ◽  
Skin Aging ◽  
Inflammatory Activity ◽  
No Production ◽  
Skin Substitutes ◽  
Kalmia Angustifolia ◽  
Isolation And Characterization ◽  
Assembly Method ◽  
Dermal Thickness ◽  
Anti Inflammatory

Skin aging is the most visible element of the aging process, giving rise to a major concern for many people. Plants from the Ericaceae family generally have antioxidant and anti-inflammatory properties, making them potential anti-aging active ingredients. This study aimed to evaluate the safety and anti-aging efficacy of a Kalmia angustifolia extract using reconstructed skin substitutes. The safety evaluation was performed using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, while the efficacy was determined by assessing antioxidant and anti-inflammatory activity and analyzing skin substitutes reconstructed according to the self-assembly method by histology and immunofluorescence staining (elastin, collagen-1, collagen-3, aquaporin-3). The cell viability assay established the safety of the extract at a concentration up to 200 μg/mL. The Oxygen Radical Absorbance Capacity (ORAC) assay and a cell-based assay using 2’,7’-dichlorofluorescein-diacetate (DCFH-DA) revealed a strong antioxidant activity with an ORAC value of 16 µmol Trolox Equivalent/mg and a half-maximal inhibitory concentration (IC50) of 0.37 ± 0.02 μg/mL, while an interesting anti-inflammatory activity was found in the inhibition of NO production, with an inhibition percentage of NO production of 49 ± 2% at 80 µg/mL. The isolation and characterization of the extract allowed the identification of compounds that could be responsible for these biological activities, with two of them being identified for the first time in K. angustifolia: avicularin and epicatechin-(2β-O-7, 4β-6)-ent-epicatechin. Histological analyses of skin substitutes treated with the extract showed an increase in dermal thickness compared with the controls. K. angustifolia extract enhanced the expression of elastin and collagen-1, which are usually decreased with skin aging. These results suggest that K. angustifolia has promising antioxidant efficacy and anti-aging potential.

scholarly journals Peeling with 25% trichloroacetic acid in the treatment of facial skin photoaging

2021 ◽  
Vol 6 (7) ◽  
Keyword(s):  
Trichloroacetic Acid ◽  
Skin Aging ◽  
Facial Skin ◽  
Treatment Results ◽  
Skin Rejuvenation ◽  
Degree Of Satisfaction ◽  
Skin Photoaging ◽  
Photo Damage ◽  
High Degree ◽  
Surgical Clinic

Introduction: The chemical peel consists of the application of chemical exfoliants that eliminate the layers of the skin so that it can regenerate with an improved texture and pigmentation. It is currently a valuable therapy in dermatology, however, few studies objectively evaluate its efficacy. Objective: To evaluate the efficacy and safety of peeling with 25% trichloroacetic acid in the treatment of facial skin rejuvenation. Method: An observational, analytical and longitudinal study was carried out in 250 patients from two hospitals (Surgical Clinic: “Hermanos Ameijeiras” and General Teaching: “Enrique Cabrera”), in the period between January 2010 and January 2020. Treatment It was applied monthly for 6 months. The final evaluation was carried out 3 months after the end of the treatment. Results: 238 women and 12 men were treated with an average age of 30.5 (± 8.4) years. After treatment, there were significant changes in the Glogau Photo Damage Scale (P=0.012), in the Lemperle Wrinkle Assessment Scale (P=0.016) and in the Global Aesthetic Improvement Scale (P=0.021). The adverse events found were burning, inflammation and scaling. The degree of satisfaction reported by the patients was good (4.4%) and very good (95.6%) (P=0.011). Conclusion: The 25% trichloroacetic acid peel proved to be effective and safe to reduce the signs of facial skin aging, associated with a high degree of patient satisfaction.

Glycolic acid attenuates UVB-induced aquaporin-3, matrix metalloproteinase-9 expression, and collagen degradation in keratinocytes and mouse skin

2019 ◽  
Vol 476 (10) ◽  
pp. 1387-1400 ◽  
Author(s):  
Sheau-Chung Tang ◽  
Lee-Chun Tang ◽  
Chin-Hung Liu ◽  
Pei-Yun Liao ◽  
Ji-Ching Lai ◽  
...  
Keyword(s):  
Glycolic Acid ◽  
Transient Receptor Potential ◽  
Mouse Skin ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Mitogen Activated Protein Kinase ◽  
Transient Receptor Potential Vanilloid ◽  
Type I ◽  
Dorsal Skin ◽  
Aquaporin 3

Abstract Ultraviolet-B exposure causes an inflammatory response, photoaged skin, and degradation of extracellular matrix proteins including collagen and elastin. The regulation of these genes was suggested as an important mechanism to attenuate skin aging. Glycolic acid (GA) is commonly present in fruits and recently used to treat dermatological diseases. We reported that GA slows down cell inflammation and aging caused by UVB. Little is known about GA retarding the skin premature senescence or how to impede these events. To investigate the potential of GA to regulate the expression of MMPs and collagen, GA was topically applied onto human keratinocytes and the C57BL/6J mice dorsal skin. In the present study, we demonstrated that GA reduced UVB-induced type-I procollagen expression and secretory collagen levels. GA reverted and dose-dependently increased the level of aquaporin-3 (AQP3), the expression of which was down-regulated by UVB. The UV-induced MMP-9 level and activity were reduced by GA pre-treatment. Concomitantly, GA reverted mitogen-activated protein kinase (MMP-9) activation and inhibited the extracellular signal-regulated kinase activation (p38, pERK) triggered by UVB. The animal model also presented that GA attenuated the wrinkles caused by UVB on the mouse dorsal skin. Finally, GA triggers the transient receptor potential vanilloid-1 (TRPV-1) channel to initiate the anti-photoaging mechanism in keratinocytes. These findings clearly indicated that the mechanisms of GA promote skin protection against UVB-induced photoaging and wrinkle formation. GA might be an important reagent and more widely used to prevent UVB-induced skin aging.

scholarly journals Skin Rejuvenation and Volume Enhancement with the Micro Superficial Enhanced Fluid Fat Injection (M-SEFFI) for Skin Aging of the Periocular and Perioral Regions

2016 ◽  
Vol 37 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Alessandro Gennai ◽  
Alessandra Zambelli ◽  
Erica Repaci ◽  
Rodolfo Quarto ◽  
Ilaria Baldelli ◽  
...  
Keyword(s):  
Skin Aging ◽  
Fat Injection ◽  
Skin Rejuvenation

scholarly journals Comparison of therapeutic effects between topical 8-oxo-2′-deoxyguanosine and corticosteroid in ocular alkali burn model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dong Hyun Kim ◽  
Sang-Taek Im ◽  
Jin Young Yoon ◽  
Seunghoon Kim ◽  
Mee Kum Kim ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Prednisolone Acetate ◽  
Therapeutic Effects ◽  
Alkali Burn ◽  
Tnf Α ◽  
Phosphate Buffered Saline

AbstractWe compared the therapeutic effects of topical 8-oxo-2′-deoxyguanosine (8-oxo-dG) and corticosteroid in a murine ocular alkali burn model. (n = 128) The corneal alkali burn model was established by applying 0.1 N sodium hydroxide (NaOH), followed by treatment with 8-oxo-dG, 0.1% fluorometholone (FML), 1% prednisolone acetate (PDE), or phosphate-buffered saline (PBS) twice daily. One week later, the clinical and histological status of the cornea were assessed. Transcript levels of inflammatory cytokines and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase as well as the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the cornea, were assayed. The 8-oxo-dG and PDE groups showed marked improvements in corneal integrity and clarity when compared with the PBS group (each p < 0.01). The numbers of cells stained for neutrophil elastase and F4/80-positive inflammatory cells were significantly decreased, with levels of interleukin(IL)-1β, IL-6, tumor necrosis factor(TNF)-α, and total ROS/RNS amounts markedly reduced in the 8-oxo-dG, FML, and PDE groups (each p < 0.05). Levels of NADPH oxidase type 2 and 4 were substantially more repressed in the 8-oxo-dG-treated group than in the PDE-treated group (each p < 0.05). Topical 8-oxo-dG showed excellent therapeutic effects that were comparable with those treated with topical PDE in a murine ocular alkali burn model.

scholarly journals A Study Comparing the Efficacy of Monopolar Radiofrequency and Glycolic Acid Peels in Facial Rejuvenation of Aging Skin Using Histopathology and Ultrabiomicroscopic Sonography (UBM) – An Evidence Based Study

2016 ◽  
Vol 59 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Deepal V. Wakade ◽  
Chitra S. Nayak ◽  
Kalpana D. Bhatt
Keyword(s):  
Glycolic Acid ◽  
Facial Rejuvenation ◽  
Collagen Remodeling ◽  
Collagen Formation ◽  
Dermal Thickness ◽  
Chemical Peels ◽  
The Face ◽  
Outer Canthus ◽  
Nasolabial Folds ◽  
Equal Efficacy

Background: Radio frequency (RF) and chemical peels have been used for nonablative skin rejuvenation. Both of these cause collagen remodeling in the dermis and neo-collagen formation resulting in facial rejuvenation. There is limited literature on the evaluation of collagen remodeling by objective methods. Objective: To compare the benefits of monopolar radiofrequency and glycolic acid peels in facial rejuvenation with regards to histopathology and Ultrabiomicroscopic sonography (UBM). Methodology: In this study, forty patients with mild to moderate photoaging received four treatments with 3 weeks interval of monopolar radiofrequency on one side of face and glycolic acid peels in increasing concentrations (NeostrataR) on the other side. Pre and post treatment, 2 mm biopsies were taken from both preauricular areas and Ultrasonography using a 35 MHz probe was done from outer canthus of eye and nasolabial folds from both sides of face. A blinded assessment was done to measure the increase in the grenz zone and dermal thickness. Results: In 35/40 patients there was a significant increase in the grenz zone on histopathology and decrease in subepidermal low-echogenic band (SLEB) on UBM of the nasolabial folds on both sides of the face (p < 0.05). Conclusion: Radiofrequency and chemical peels showed equal efficacy in the treatment of facial rejuvenation.

scholarly journals Adipose Collagen Fragment: an Injectable Sustained-release Collagen Scaffold of Adipokines for Preventing Skin Photoaging in Mice

2021 ◽  
Author(s):  
Xiaoxuan Jin ◽  
Yuchen Zhang ◽  
Xiangdong Zhang ◽  
Yibao Li ◽  
Mimi Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sustained Release ◽  
Collagen Synthesis ◽  
Reactive Oxygen ◽  
Collagen Scaffold ◽  
Collagen Degradation ◽  
Oxygen Species ◽  
Dermal Thickness ◽  
Skin Photoaging ◽  
Collagen Fragment

Abstract Objective: Ultraviolet A (UVA) radiation is the major contributor to skin photoaging, associated with increased collagen degradation and reactive oxygen species expression. Adipokines have been proven as promising therapeutic agents for skin photoaging. However, adipokine therapy is generally limited by the short in vivo release duration and biological instability. Therefore, developing a treatment that provides a sustained release of adipokines and enhanced therapeutic effects is desirable. Methods: Adipose collagen fragment (ACF) was prepared from lipoaspirate and characterized. The injectability, collagen components, and adipokine release pattern of ACF were identified in vitro. Then, we evaluated its therapeutic efficacy by injecting ACF and phosphate-buffered saline, as a control, into the dermis of photoaging nude mice and harvesting skin samples at weeks 1, 2, and 4 after treatment for assessment. The collagen synthesis and collagen degradation in ACF implants were evaluated by immune staining. Dermal thickness, fibroblast expression, collagen synthesis, reactive oxygen species level, antioxidase expression, capillary density, and apoptotic cell number were evaluated by histological assessment, immune staining, and polymerase chain reaction in the skin samples.Results: ACF is the concentrated adipose extracellular matrix collagen fragment without viable cells and can be injected through fine needles. ACF undergoes collagen degradation and promotes neocollagen synthesis in ACF implants. Meanwhile, ACF serves as a sustained-release system of adipokines and exhibits a significantly higher therapeutic effect on mouse skin photoaging compared to controls. ACF increases the dermal thickness, improves fibroblast expression and promotes collagen synthesis. ACF treatment of UVA-irradiated skin reduces reactive oxygen species expression, decreases the number of apoptotic cells, improves capillary density and promotes the expression of antioxidase superoxide dismutase-1, catalase and glutathione peroxidase-1 by sustainedly releasing adipokines.Conclusions: ACF is an adipokines-enriched, sustained-release extracellular matrix collagen scaffold that can prevent UVA-induced skin photoaging in mice. ACF may serve as a novel autologous skin filler for skin rejuvenation applications in the clinic.

scholarly journals Skin Rejuvenation Profile in Dr. Soetomo General Hospital Surabaya

2019 ◽  
Vol 3 (12) ◽  
pp. 475-479
Author(s):  
Lutfia Ariska Ramadhani ◽  
Trisniartami Setyaningrum ◽  
Etty Hary Kusumastuti
Keyword(s):  
General Hospital ◽  
Aging Process ◽  
Uv Light ◽  
Intrinsic Factor ◽  
Skin Aging ◽  
The Body ◽  
Skin Rejuvenation ◽  
Extrinsic Factors ◽  
Tissue Changes ◽  
Poor Nutrition

Skin aging is a condition in which cell and tissue changes occur due to mechanism abnormalities and a decrease in function of a tissue which can be triggered by intrinsic or extrinsic factors. Intrinsic factor is an aging process which originates from the body of an individual itself while extrinsic factor is an aging process caused by factors from the outside, such as excessive exposure to the UV light, smoking, or poor nutrition. Skin rejuvenation therapy, hopefully, would be able to restore or even slow down the aging process itself. This research was a descriptive observational using retrospective approach based on patients’ medical record in the Outpatient Clinic of Dermatology and Venereology Department in Dr. Soetomo General Hospital Surabaya in January to December 2017. This study obtained 203 samples (198 women/females and 5 men/males) with the most age group ranging from 45-

Sign in / Sign up

Export Citation Format

Share Document