Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions

2020 ◽  
Author(s):  
Hana Akari Arifin ◽  
Takuyu Hashiguchi ◽  
Kiyoko Nagahama ◽  
Masatsugu Hashiguchi ◽  
Melody Muguerza ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Luciferase Reporter ◽  
Soybean Seeds ◽  
Flavonoid Content ◽  
Varietal Differences ◽  
Estrogenic Effects ◽  
Seed Content ◽  
Responsive Element ◽  
Antioxidant Responsive Element

ABSTRACT Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity.

scholarly journals Antioxidative Activities and Active Compounds of Extracts fromCatalpaPlant Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hongyu Xu ◽  
Gege Hu ◽  
Juane Dong ◽  
Qin Wei ◽  
Hongbo Shao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Crude Extracts ◽  
Catalpa Ovata ◽  
High Antioxidant Activity ◽  
Water Fractions

In order to screen theCatalpaplant with high antioxidant activity and confirm the corresponding active fractions fromCatalpa ovataG. Don,C. fargesiiBur., andC. bungeiC. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions ofCatalpaplant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content inC. bungeiC. A. Mey. (30.07 mg/g·DW) was the highest, followed by those inC. fargesiiBur. (25.55 mg/g·DW) andC. ovataG. Don (24.96 mg/g·DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves ofC. bungeiC. A. Mey., the total flavonoid content and antioxidant activity in crude extracts fromC. bungeiC. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed byn-butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1) and apigenin (2), respectively.

scholarly journals Total Flavonoid Content and Antioxidant Activity of Temu Mangga (Curcuma mangga Val. & Zijp) and its Classification with Chemometrics

2020 ◽  
pp. 4
Author(s):  
Yuniar Intan Hartono ◽  
Indah Widyastuti ◽  
Hanna Zaidah Luthfah ◽  
Rosy Islamadina ◽  
Adelin Theresia Can ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
The Relationship ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Indonesia is a rich country in terms of its biodiversity. Herbs that are widely used tomaintain a healthy body comes from the Zingiberaceae family, including temu mangga. Temu mangga(Curcuma mangga Val. & Zijp) is effective for relieving gastric pain, diarrhea, menstrual pain, acneand ulcers, and increasing appetite. Besides, temu mangga is considered to have antioxidant activitydue to the presence of the phenolic and flavonoid compounds. This study aims to determine thecorrelation and classification of temu mangga from various places based on total flavonoid contentand antioxidant activities. The samples were extracted with methanol, then tested for totalflavonoid content by AlCl3 method and antioxidant activity by DPPH free radical capture method.Analysis was performed using correlation coefficient value and chemometrics PCA (PrincipalComponent Analysis) and CA (Cluster Analysis) to see the relationship between total flavonoidcontent with their antioxidant activity and to see their classification. Total flavonoid content ofmethanolic extract of temu mangga gives a moderate correlation to its antioxidant activity with r =-0.6085 and clustering samples successfully determined by PCA and CA which resulted in 4 groups.Keywords: Curcuma mangga Val. & Zijp, Methanolic Extract, Flavonoid, Antioxidant, PCA, CA

scholarly journals Hemin-Mediated Regulation of an Antioxidant-Responsive Element of the Human Ferritin H Gene and Role of Ref-1 during Erythroid Differentiation of K562 Cells

2006 ◽  
Vol 26 (7) ◽  
pp. 2845-2856 ◽  
Author(s):  
Kenta Iwasaki ◽  
Elizabeth L. MacKenzie ◽  
Kiros Hailemariam ◽  
Kensuke Sakamoto ◽  
Yoshiaki Tsuji
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
K562 Cells ◽  
Erythroid Differentiation ◽  
Luciferase Reporter ◽  
H Gene ◽  
Human Ferritin ◽  
Responsive Element ◽  
Ferritin H ◽  
Antioxidant Responsive Element

ABSTRACT An effective utilization of intracellular iron is a prerequisite for erythroid differentiation and hemoglobinization. Ferritin, consisting of 24 subunits of H and L, plays a crucial role in iron homeostasis. Here, we have found that the H subunit of the ferritin gene is activated at the transcriptional level during hemin-induced differentiation of K562 human erythroleukemic cells. Transfection of various 5′ regions of the human ferritin H gene fused to a luciferase reporter into K562 cells demonstrated that hemin activates ferritin H transcription through an antioxidant-responsive element (ARE) that is responsible for induction of a battery of phase II detoxification genes by oxidative stress. Gel retardation and chromatin immunoprecipitation assays demonstrated that hemin induced binding of cJun, JunD, FosB, and Nrf2 b-zip transcription factors to AP1 motifs of the ferritin H ARE, despite no significant change in expression levels or nuclear localization of these transcription factors. A Gal4-luciferase reporter assay did not show activation of these b-zip transcription factors after hemin treatment; however, redox factor 1 (Ref-1), which increases DNA binding of Jun/Fos family members via reduction of a conserved cysteine in their DNA binding domains, showed induced nuclear translocation after hemin treatment in K562 cells. Consistently, Ref-1 enhanced Nrf2 binding to the ARE and ferritin H transcription. Hemin also activated ARE sequences of other phase II genes, such as GSTpi and NQO1. Collectively, these results suggest that hemin activates the transcription of the ferritin H gene during K562 erythroid differentiation by Ref-1-mediated activation of these b-zip transcription factors to the ARE.

scholarly journals Effect of extraction temperature and time on the extraction of phenolic compounds and antioxidant capacity of Phaleria macrocarpa fruit

2018 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Peng Yee Lim ◽  
Sook Fun Pang ◽  
Mashitah Mohd Yusoff ◽  
Jolius Gimbun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Pearson Correlation Coefficient ◽  
Flavonoid Content ◽  
Phaleria Macrocarpa ◽  
Optimum Extraction

Phaleria macrocarpa (ver. name: ‘mahkota dewa’) is a plant which has many medically useful antioxidant activities (Anggraini & Lewandowsky, 2015). The polyphenols responsible for this antioxidant activity has to be extracted before it can be routinely used (Shwter et al., 2016). This study investigates the extraction of polyphenols from P. macrocarpa fruits and its antioxidant activity (DPPH-RSA) under influence of extraction time and temperature. By employing maceration technique, the P. macrocarpa fruits extract showed the maximum total phenolic content (TPC), total flavonoid content (TFC) and DPPH-RSA with value of 69.5 mg QE/g DW, 183.2 mg GA/g DW and 171. 8 mg BHA/g DW, respectively at  optimum extraction conditions of 60 min and 80 ºC (Fig. 1). Excellent and positive Pearson correlation coefficient with R2 > 0.91 between the TPC, TFC and antioxidant activities was observed.

Comparative Antioxidant Potential of Bark and Leaves of Terminalia arjuna (Roxb) Wight & Arn from Himachal Pradesh

2017 ◽  
Vol 6 (1) ◽  
pp. 27 ◽  
Author(s):  
Vikas Kumar ◽  
Kamal Dev ◽  
Anuradha Sourirajan ◽  
Prem Kumar Khosla
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Terminalia Arjuna ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

The present study aimed to compare antioxidant activities, total phenolic and flavonoid content present in leaves and bark of Terminalia arjuna and check whether there is any correlation between phenolic content and flavonoid content with antioxidant activities or not. Phytochemical screening of ethanolic extract of leaves and bark revealed the presence of phenols, flavonoids, tannins, carbohydrates, glycosides, saponin, phytosterols and phytosteroids. Total phenolic content was found to be higher in bark (272.71±3.18 mg/g gallic acid equivalents) as compared to that of leaves (95±3.11 mg/g gallic acid equivalents). Similarly, flavonoid content of ethanolic extract of bark was found to be higher (203.95±5.13 mg/g rutin equivalents) than that of leaves (87.625±4.28 mg/g rutin equivalents). DPPH activity of ethanolic extract of bark (IC50-17.41 µg/ml) was more than that of leaves (IC50-20.22µg/ml). FRAP activity of bark (IC50- 4.781 µM Fe (II) equivalents) is more than that of leaves (IC50-7.572 µM Fe (II) equivalents). Nitric oxide (NO) scavenging activity of bark (IC50-12.87 µg/ml) was higher than that of leaves (IC50-13.91 µg/ml).The present study clearly showed that there is a correlation between total phenolics, flavonoid contents and antioxidant activity of leaves and bark of T. arjuna. The phenolic compounds and flavonoids could be major contributor to antioxidant activity of T. arjuna. Keywords: Terminalia arjuna, DPPH, FRAP, NO, IC50, Antioxidants

scholarly journals FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

2016 ◽  
Vol 21 (3) ◽  
pp. 767-779 ◽  
Author(s):  
Renata Nunes Oliveira ◽  
Maurício Cordeiro Mancini ◽  
Fernando Cabral Salles de Oliveira ◽  
Thayse Marques Passos ◽  
Brid Quilty ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Natural Products ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
The Other ◽  
Ftir Analysis ◽  
Flavonoid Content ◽  
Dragon’S Blood ◽  
Brazilian Propolis

ABSTRACT Natural products are used in wound healing in order to prevent infection. Propolis is a well known antimicrobial with phenolic compounds and flavonoid content which vary according to the propolis origin. Besides propolis (from both Brazilian and UK sources), pomegranate, dragon's blood and sage are possible antimicrobials to be used in biomaterials. The goal of this work was to analyze the amount of phenols and flavonoid compounds in these natural products, their antioxidant activities and the bonds present by FTIR. The FTIR analysis revealed the presence of active compounds in all drug samples. The phenols quantification showed that Brazilian propolis was rich in phenols compared to the other drugs, followed by pomegranate and UK propolis. UK propolis was the most rich in flavonoids, which is expected on account of its origin. Pomegranate, UK propolis and Dragon's blood presented the highest antioxidant activity. All samples presented antioxidant activity > 82%.

scholarly journals Characteristics Evaluation of Commercial Fruit Tea Extracts in Korea

2021 ◽  
Vol 19 (4) ◽  
pp. 693-703
Author(s):  
Ah-Ra Hwang ◽  
Myung-Ryun Han ◽  
Ae-Jung Kim
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Physicochemical Characteristics ◽  
Hot Water ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Tea Extract

Purpose: We evaluated the physicochemical characteristics and antioxidant activity of 21 types of fruit tea extract that are consumed in Korea. The data were analyzed and curated for the development of processed products using tea in the future.Methods: After extraction using hot water at 70℃, we analyzed the physicochemical properties including color and pH, as well as the antioxidant activity (total flavonoid content, DPPH scavenging activity, and α-glucosidase inhibitory activity).Results: Calamansi tea exhibited the lowest brightness with a beet-red appearance, and a yellowness that was significantly higher in jujube and radish. The pH ranged from 3.04 to 7.18 and most samples were in an acidic state. Of these, the pH of schizandra fruit tea was the lowest at 3.04±0.01. The total flavonoid content was the highest in calamansi at 217.87±1.45 QE/g, whereas that of cacao nibs was the lowest at 29.04±0.70 QE/g. The α-glucosidase inhibitory activity of Chinese quince was the highest at 59.96%±0.22%.Conclusion: We obtained basic data for several fruit tea extracts by analyzing the physicochemical properties and antioxidant activities. We anticipate that these results will represent useful data for blending fruits for tea product development.

scholarly journals Antioxidant Activity and Phytochemical Screening of Costus pictus D. Don Leaf Extracts

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Naik A. ◽  
Krishnamurthy R.i ◽  
Pathak J.
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Quality Health ◽  
Physiological And Biochemical

The occurrence of chronic diseases is rapidly growing worldwide. Diet and nutrition are significant aspects in the promotion and maintenance of good quality health throughout the whole life span. Overproduction of liberated radicals occurred due to physiological and biochemical changes in our body and then can cause oxidative damage to biocompounds of human body (e.g. lipids, proteins, DNA). Medicinal plant based products exert beneficial properties such as antioxidant, anticancer, hypoglycemic and hypolipidaemic activities so their utilization is increased recently. This study includes preliminary phytochemical screening and quantification of phenol and flavonoid of Costus pictus aqueous (CPAQ) and methanolic (CPME) extracts. DPPH, FRAP, Fe3+ reducing power, and superoxide scavenging assay were used for the estimation of antioxidant potential of leaf extracts CPAQ and CPME. CPME gave the elevated amount of phenol and flavonoid content and antioxidant activities. The antioxidant activity of Costus pictus extracts significantly correlated (P less than 0.05) with the phenolic content. Ascorbic acid used as standard compound and showed maximum antioxidant power in this study.

scholarly journals UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK BUAH DAN KULIT PISANG JARUM (Musa acuminata var. Jarum (AA Group))

2019 ◽  
Vol 9 (1) ◽  
pp. 60-69
Author(s):  
Fensia Analda Souhoka ◽  
Yeanchon H Dulanlebit ◽  
Ester M. C Tomasoa
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Musa Acuminata ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Peel Extract

This study aimed to determine phytochemical tests and antioxidant activity of fruit and peel extract of pisang jarum. Sample was extracted with 80% of ethanol, methanol, and acetone using reflux method. The result showed that pisang jarum extract contains phenols, flavonoids, and terpenoids, whereas peel extract contains phenols, flavonoids, terpenoids, alkaloids, and saponins. The total phenol content (TPC) of pisang jarum is 0.0250-0.0316 mg PE/g extract and peel is 0,1756-0,2679 mg PE/g extract. The total flavonoid content (TFC) of pisang jarum is 0.8412-1.4466 mg QE/g extract and peel is 3.2128-5.1073 mg QE/g extract. The antioxidant activity of extract were analyze by DPPH free radical scavenging activity. The antioxidant activities (IC50) of pisang jarum is 1092.92-3871.24 ppm and peel is 310.08-558.07 ppm, so it is classified as a weak antioxidant

scholarly journals Evaluation of Galantamine, Phenolics, Flavonoids and Antioxidant Content of Galanthus Species in Turkey

2019 ◽  
pp. 1-12 ◽  
Author(s):  
Ibrahim Bulduk ◽  
Yasemin Sunucu Karafakıoğlu
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
Antioxidant Activities ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Content ◽  
Galanthus Nivalis

Aims: The aim of the present study was to determine the total phenolic and flavonoid content and antioxidant activities in Galanthus species (Gaalanthus woronowii, Galanthus nivalis, and Galanthus elwesii) indigenous to Turkey. Study Design: The plant materials used in the study, Galanthus elwesii samples were collected in Antalya province, Galanthus nivalis samples were collected in Istanbul province, and Galanthus woronowii samples were collected in Çaykara, Trabzon province in September 2018. Place and Duration of Study: Plant samples were stored in Herbarium Material Warehouse at Afyon Kocatepe University. The plant leaves and grated bulbs were dried in an incubator at 60°C. The bulb and leaf samples were then pulverized to 80 mesh particle size for analysis. Methodology: Total phenolic content was determined spectrophotometrically with Folin-Ciocalteu procedure and calculated as gallic acid equivalent (GAE). Total flavonoid content was determined with aluminum chloride colorimetric method and calculated as catechin equivalent (CAE). Antioxidant activities were determined with TEAC (Trolox equivalent antioxidant capacity) and DPPH (diphenyl-p-picrylhydrazyl radical) methods. The phenolic acid and galantamine content were determined by reversed phase HPLC. Results: The highest total flovanoid content was determined as 33 mg CAE/g DW in Galanthus woronowii leaves and as 27 mg CAE / g DW in bulbs. DPPH removal activity was 77% in 500 μg/mL Galanthus woronowii leaf extract concentration and 93% in the ascorbic acid control group. The highest antioxidant content was observed in the leaves of Galanthus woronowii as 23 µmol Trolox/100 g DW and as 21 µmolTrolox/100 g DW in the bulbs. Higher galantamine content was determined in aerial parts (leaves) when compared to the underground parts (bulbs). The galantamine content in the leaves of all three Galanthus species was about 0.082%. The galantamine content in the bulbs of all three species was about 0.045%. Gallic, protocatechic, vanilic, caffeic, syringic, rosmarinic acid and catechin were identified in the leaves and bulbs of the three species with HPLC phenolic acid analysisIt was determined that the major phenolic acid was gallic acid. Conclusion: The present study findings demonstrated that Galantthus species has antioxidant capacity. Galanthus spp. leaves had higher antioxidant activity when compared to the bulbs. Galanthus woronowii exhibited the highest antioxidant activity among the scrutinized species.

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