scholarly journals Evaluation of Antioxidant Capacity, Fat-Soluble Micronutrients and Glucosinolates at Different Growth Stages of a Commonly-Consumed Asian Brassica Vegetable

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 446-446
Author(s):  
Choon Nam Ong ◽  
Wee Kee Tan ◽  
Chiang Shiong Loh
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Plant Development ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Mature Stage ◽  
Growth Stages ◽  
Vitamin K1 ◽  
Leaf Stage ◽  
Total Antioxidant ◽  
Different Growth Stages

Abstract Objectives This study aimed to systematically investigate a comprehensive list of bioactive components in a commonly consumed Asian leafy vegetable, Brassica rapa. They included polyphenols, fat soluble micronutrients such as carotenoids, tocopherols and phylloquinone, and various glucosinolates (GLs), in addition to the total antioxidant capacity, at three different growth stages. Methods Phenolic compounds were measured using C-18 HPLC/MS. Carotenoids, vitamin E and phylloquinone (vitamin K1) were detected using RP-30-HPLC/MS. Glucosinolates were determined using HILIC-HPLC/MS. Different AOX capacities were assessed either using UV-Visible or Fluorescent Spectrophotometer. Results Phenolic compounds, such as total flavonoid and hydroxycinnamic acids, were highest at 3-leaf stage and significantly lower in mature plant. The main carotenoids, lutein and β-carotene, remained the same throughout the different growth stages. However, violaxanthin, neoxanthin and β-cryptoxanthin were higher also at 3-leaf stage, and decrease gradually with growth. In contrast, tocopherol concentration continued to increase and was highest at mature stage. There was little change for phylloquinone (vitamin K1) throughout the three studied stages. For the unique components in Brassicaeae, both dominating aliphatic and indolic-GLs concentrations were significantly higher at 1-leaf stage than at mature stage ( > 30x), whereas the aromatic GLs, although of much lower concentrations, continued to increase throughout the growth, and with highest amount at mature stage, suggesting that there is metabolic pathway shift of these secondary metabolites during plant development. In general, antioxidant capacity measured using Scavenging of DPPH radicals, Trolox Equivalent Antioxidant Capacity and Oxygen Radical Absorbance Capacity suggested that there was an insignificant increase of total antioxidant content from seedling to mature vegetable. Conclusions The overall findings suggest that most of the nutritional active bio-components were higher at younger stages, either at 1-leaf or 3-leaf stage, except α-tocopherol, with continual accumulation during plant development. These comprehensive profiles of various nutrients should be valuable to the nutritional community. Funding Sources National Research Foundation, Prime Minister's Office, Singapore.

scholarly journals Actinidia arguta Leaf as a Donor of Potentially Healthful Bioactive Compounds: Implications of Cultivar, Time of Sampling and Soil N Level

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3871
Author(s):  
Jan Stefaniak ◽  
Barbara Łata
Keyword(s):  
Antioxidant Capacity ◽  
Plant Development ◽  
Growing Season ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Development Phase ◽  
Soil N ◽  
Actinidia Arguta ◽  
N Level ◽  
N Fertility

The aim of this study was to assess the enzymatic and non-enzymatic antioxidant status of kiwiberry (Actinidia arguta) leaf under different N regimes tested three times in field conditions during the 2015 growing season in two cultivars (‘Weiki’ and ‘Geneva’). Leaf total antioxidant capacity using ABTS, DPPH and FRAP tests was evaluated in the years 2015 to 2017, which experienced different weather conditions. Both cultivars exhibited a significant fall in leaf L-ascorbic acid (L-AA) and reduced glutathione (GSH) as well as global content of these compounds during the growing season, while total phenolic contents slightly (‘Weiki’) or significantly (‘Geneva’) increased. There was a large fluctuation in antioxidative enzyme activity during the season. The correlation between individual antioxidants and trolox equivalent antioxidant capacity (TEAC) depended on the plant development phase. The study revealed two peaks of an increase in TEAC at the start and end of the growing season. Leaf L-AA, global phenolics, APX, CAT and TEAC depended on the N level, but thiol compounds were not affected. Over the three years, TEAC decreased as soil N fertility increased, and the strength of the N effect was year dependent. The relationship between leaf N content and ABTS and FRAP tests was highly negative. The antioxidant properties of kiwiberry leaves were found to be closely related to the plant development phase and affected by soil N fertility.

scholarly journals Quantitative Response of Maize Vcmax25 to Persistent Drought Stress at Different Growth Stages

Water ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 1971
Author(s):  
Xingyang Song ◽  
Guangsheng Zhou ◽  
Qijin He ◽  
Huailin Zhou
Keyword(s):  
Drought Stress ◽  
Early Warning Systems ◽  
Growth And Yield ◽  
Leaf Water Content ◽  
Growth Stages ◽  
Leaf Stage ◽  
Maximum Value ◽  
Maize Varieties ◽  
Persistent Drought ◽  
Different Growth Stages

Drought stress has adverse effects on crop growth and yield, and its identification and monitoring play vital roles in precision crop water management. Accurately evaluating the effect of drought stress on crop photosynthetic capacity can provide a basis for decisions related to crop drought stress identification and monitoring as well as drought stress resistance and avoidance. In this study, the effects of different degrees of persistent drought in different growth stages (3rd leaf stage, 7th leaf stage and jointing stage) on the maximum carboxylation rate at a reference temperature of 25 °C (Vcmax25) of the first fully expanded leaf and its relationship to the leaf water content (LWC) were studied in a field experiment from 2013 to 2015. The results indicated that the LWC decreased continuously as drought stress continued and that the LWC decreased faster in the treatment with more irrigation. Vcmax25 showed a decreasing trend as the drought progressed but had no clear relationship to the growth stage in which the persistent drought occurred. Vcmax25 showed a significantly parabolic relationship (R2 = 0.701, p < 0.001) with the LWC, but the different degrees of persistent drought stress occurring in different growth stages had no distinct effect on the LWC values when Vcmax25 reached its maximum value or zero. The findings of this study also suggested that the LWC was 82.5 ± 0.5% when Vcmax25 reached its maximum value (42.6 ± 3.6 μmol m−2 s−1) and 67.6 ± 1.2% (extreme drought) when Vcmax25 reached zero. These findings will help to improve crop drought management and will be an important reference for crop drought identification, classification and monitoring as well as for the development of drought monitoring and early warning systems for other crops or maize varieties.

scholarly journals Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Martina Horvathova ◽  
Zuzana Orszaghova ◽  
Lucia Laubertova ◽  
Magdalena Vavakova ◽  
Peter Sabaka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Healthy Volunteers ◽  
Total Antioxidant Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

scholarly journals Changes in essential oil compositions, total phenol, flavonoids and antioxidant capacity of Achillea millefolium at different growth stages

2020 ◽  
Vol 152 ◽  
pp. 112570 ◽  
Author(s):  
Nasrin Farhadi ◽  
Khyrollah Babaei ◽  
Sara Farsaraei ◽  
Mohammad Moghaddam ◽  
Abdollah Ghasemi Pirbalouti
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Total Phenol ◽  
Achillea Millefolium ◽  
Growth Stages ◽  
Different Growth Stages

Biochemical components of advanced selections and raspberry cultivars

2008 ◽  
Vol 88 (1) ◽  
pp. 175-178 ◽  
Author(s):  
B. Ehsani-Moghaddam ◽  
S. Khanizadeh ◽  
J. A. Sullivan
Keyword(s):  
Antioxidant Capacity ◽  
Rubus Idaeus ◽  
Soluble Solids ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Content ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Lipophilic Antioxidant ◽  
Solids Content ◽  
The University

Commercially grown raspberry cultivars (Rubus idaeus L.) (Festival, Boyne, Nova and Killarney) and advanced selections (88–18, 88–117, 88–134, SJR942-7) from the University of Guelph and Agriculture and Agri-Food Canada (AAFC) were evaluated for total antioxidant capacity, soluble solids content and acidity. The crude, hydrophilic and lipophilic antioxidant content of berries were measured using the Trolox equivalent antioxidant capacity (TEAC) method. Significant variations were observed among crude, hydrophilic and lipophilic antioxidant content of the different advanced selections. Selection 88–18, had the highest crude, hydrophilic and lipophilic antioxidant capacity, while SJR942-7 had the highest soluble solids. The study shows the importance of genetic background in determining the antioxidant potential in raspberry and the possibility of developing new lines rich in phytochemicals. Key words: Hydrophilic, lipophilic, total antioxidants, TEAC, Trolox, soluble solids

scholarly journals Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes

Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 87-100 ◽  
Author(s):  
Sladjana Zilic ◽  
Vesna Hadzi-Taskovic-Sukalovic ◽  
Dejan Dodig ◽  
Vuk Maksimovic ◽  
Vesna Kandic
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Phenolic Acids ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Wheat Genotypes ◽  
Total Antioxidant ◽  
Bound Phenolics

The objective of this study was to determine phenolic compounds and the total antioxidant capacity in the grain of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) wheat genotypes. Soluble free forms of total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids were investigated. In addition, the correlation coefficients between total antioxidant capacities and the concentration of different soluble free phenolic compounds, as well as between soluble free total phenolics and phenolic acids, flavonoids and PVPP bound phenolics were determined. Significant differences in the content of aceton/water extractable total phenolics, PVPP bound phenolics and phenolic acids between and within two wheat species were found. On the average, durum wheat samples had about 1.19-fold higher total phenolic compounds and about 1.5-fold higher PVPP bound phenolics than bread wheat samples. Three phenolic acids, ferulic, caffeic and chlorogenic, were detected in wholemeal bread wheat. Caffeic acid was not found in durum wheat samples whilst ferulic acid was the most abundant. Proanthocyanidins in bread and durum wheat genotypes were not detected. The antioxidant capacity measured as the DPPH radical scavenging activity was similar in wholemeal of bread and durum wheat, however, significant differences were observed among genotypes within species.

scholarly journals Association of Dietary Total Antioxidant Capacity and MC4R Polymorphisms in Metabolically Healthy and Unhealthy Overweight/Obese Women

2021 ◽  
Author(s):  
maryam elhamkia ◽  
Leila Setayesh ◽  
Habib Yarizadeh ◽  
Sara Pooyan ◽  
Zahra Veysi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Metabolic Health ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Obese Women ◽  
Cross Sectional ◽  
Reducing Ability ◽  
Total Antioxidant ◽  
Metabolically Healthy ◽  
Dietary Total Antioxidant Capacity

Abstract Background: 3-15% of people with obesity display a metabolically healthy phenotype. An inflammatory diet impairs metabolic signaling pathways and eliciting metabolic syndrome. The main purpose of this study was to investigate how the Dietary Total Antioxidant Capacity (DTAC) and the MC4R variants affect the metabolic health status in overweight and obese Iranian women.Methods and results: This cross-sectional study was conducted on 237 overweight-obese Tehrani women with mean age of 36 years.The DTAC was calculated using the following indices: total reactive antioxidant potential (TRAP), Trolox equivalent antioxidant capacity (TEAC), and the ferric-reducing ability of plasma (FRAP). The Metabolic health was evaluated using the Karelis criteria. The MC4R genotypes were determined by the restriction fragment length polymorphism (PCR-RFLP) method. Approximately 43% of participants were identified as Metabolically Unhealthy Obesity (MUO), 6% Metabolically Healthy Obesity (MHO) including 42.5% of the total had T/T genotype ,23.8% had the C/T genotype, and 33.5% had the C/C genotype (P=0.05). In the C/C genotype people 75% were unhealthy whereas it was lower in T/T and T/C genotype people. Further, The C/C genotype with higher DTAC quartile had higher Karlis score than other DTAC quartile. A liner model test supported that the probability of MHO was significantly higher in subjects with the T/C genotype (β=-0.001, 95% CI= -0.001-4.796; P ≤ 0.1). Conclusions: Our results indicated that the C/C genotype with higher DTAC had favorable lipid profile and were metabolically healthier. It is plausible that dietary modification targeting antioxidant intake may attenuate metabolic impairments associated with genetic predisposition.

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