scholarly journals Measurement of Nutritional and Inflammatory Biomarkers in Dry Plasma Spots (DPS) (P10-097-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Neal Craft ◽  
Francisco Arredondo ◽  
Matthew Fleshman ◽  
Eleanor Brindle ◽  
Lorraine Lillis ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Storage Temperature ◽  
Nutritional Assessment ◽  
Serum Vitamin ◽  
Temperature Stability ◽  
Retinol Binding Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Funding Sources ◽  
Gates Foundation

Abstract Objectives Conduct time and temperature stability experiments on Nutritional Biomarkers (NBs) and inflammation markers extracted from ViveBio Dry Plasma Spots (DPS). Methods Blood samples of 3 hematocrits (20, 30 and 45%) were created by combining purchased red blood cells (RBC) and sera. Blood samples of 35 µL were loaded onto ViveBio plasma separators. The underlying pads with plasma were stored at 45°, 23°, 4°, -20° and -70°C. Samples were tested on days 0, 1, 3, 7, 14, 28, 84 and 168. Ferritin, Retinol Binding Protein (RBP4), soluble Transferrin Receptors (sTfR), Thyroglobulin (Tg), α-acid Glycoprotein (AGP), and C-Reactive Protein (CRP) were measured using the Quansys Biosciences, Q-Plex Micronutrient Array (Q-Plex). Values measured in the liquid sera were used as reference values for the DPS. Red blood cell (RBC) folates and serum vitamin B12 were measured using a 96 well plate microbiological assay (ALPCO-Immundiagnostik AG). 25OH-Vitamin D3, Retinol, Methylmalonic acid (MMA) and Homocysteine (HCY) were measured using LC-MS/MS. The Q-Plex was compared at 3 independent laboratories using a group of 80 whole blood samples, non-spiked and spiked with AGP, CRP, HRP2 and sTfR. Liquid plasma and extracted plasma from ViveBio pads were generated and stored at -80°C four weeks prior to assay. Results Overall, the measured values for Ferritin, RBP4, Tg, AGP, CRP, 25OH-D, Retinol and MMA decrease with increasing storage temperature and length of storage. Ferritin and Tg exhibited the best stability at 16.8% and 8.0% over a 6 month period. Vitamin B12 exhibited very poor stability declining to below the LOQ within day 3. Most of the NBs at 1 month correlated well with liquid plasma values (r2 = 0.42 to 0.99) (AGP at 23°C, r2 = 0.428; at -70°C r2 = 0.990 Ferritin stored at 23°C, r2 = 0.854; at -70°C r2 = 0.992; Thyroglobulin at 23°C, r2 = 0.916; at -70°C r2 = 0.830). Conclusions We have demonstrated that NBs can be analyzed in low volumes of DPS generated by the ViveBio plasma separator. The Q-Plex performed reproducibly on split samples among 3 labs. These tools offer great potential to improve nutritional assessment in population studies. Funding Sources Funding provided by the Bill and Melinda Gates foundation.

scholarly journals Response of Nutritional Biomarkers in Bangladeshi Subjects Given an Immunological Challenge (P10-096-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Neal Craft ◽  
Francisco Arredondo ◽  
Matthew Fleshman ◽  
Shaikh Meshbahuddin Ahmad ◽  
Jahangir Alam ◽  
...  
Keyword(s):  
Inflammatory Markers ◽  
Accurate Determination ◽  
Retinol Binding Protein ◽  
Post Inoculation ◽  
Retinol Binding Protein 4 ◽  
Reactive Protein ◽  
Funding Sources ◽  
Before And After ◽  
Gates Foundation ◽  
L 14

Abstract Objectives Unreliability of nutritional biomarkers (NB) have been reported in apparently healthy people with subclinical inflammation. The objective of this study was to measure NBs before and after a vaccination induced inflammation. NBs included: Ferritin (Fer), soluble Transferrin Receptor (sTfR), Retinol Binding Protein 4 (RBP4), Thyroglobulin (Tg), 25OH-Vitamin D3 (25OH-D), retinol (VA), Folates, Cobalamins (B12), and inflammatory markers, acid 1- α-Glycoprotein (AGP) and C-Reactive Protein (CRP). Methods Children aged 12–18 months were recruited at ICDDR-B in Dhaka, Bangladesh. Blood samples were drawn initially and 1 day post inoculation with Pentavalent vaccine. Harvested serum was frozen and transported to labs for analysis. Fer, sTfR, RBP4, Tg, AGP, and CRP, were measured in 96-well format by chemiluminescent with the Quansys Human Micronutrient 7 Plex array and the Q-view reader. Serum Folates and Total B12 were estimated microbiologically using ALPCO-Immundiagnostik 96-well kits. Serum 25OH-D and VA were measured by LC-MS/MS. Results Vaccine induced inflammation significantly increased CRP, AGP, Fer and sTFR by 4.48 mg/L (798%), 0.435 g/L (48%), 9.57 µg/L (60%) and 7.6 mg/L (31%), respectively. Furthermore, inflammation significantly reduced VA, RBP4, and B12 by 74.7 µg/L (18%), 0.637 mmol/L (34%), and 38.74 ng/L (14%), respectively. Tg, Folates and 25OH-D were not significantly affected by the vaccine induced inflammatory response. Conclusions The study confirms that mild inflammation significantly alters the reliability of 7 out of 10 measured nutritional biomarkers after vaccine inoculation. The measurement of inflammatory markers is necessary for accurate determination of nutritional status in young children. Funding Sources Funding provided by the Bill and Melinda Gates Foundation.

scholarly journals Quadruple Fortification of Salt with Iodine, Iron, Vitamins B9 and B12 to Reduce Maternal and Neonatal Mortality by Reducing Anemia and Nutritional Deficiency Prevalence (P24-041-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Venkatesh Mannar ◽  
Levente Diosady
Keyword(s):  
Folic Acid ◽  
Vitamin B12 ◽  
Pilot Scale ◽  
Iron Core ◽  
Ferrous Fumarate ◽  
Funding Sources ◽  
Spray Solution ◽  
Solid Iron ◽  
Gates Foundation ◽  
Average Size

Abstract Objectives To develop a quadruple fortified salt(QFS) formulation that provides100 + % of RDA for iodine and vitamin B12 and 30–50% of RDA for iron and folic acid in forms that are organoleptically stable, bioavailable and acceptable to consumers Methods Iodine was sprayed onto salt as an aqueous solution of potassium iodate. Iron was admixed as a solid premix, which was colour masked with TiO2 and encapsulated in soy stearine to provide a water-impervious coating. The iron core was made of ferrous fumarate, which was agglomerated to an average size matching salt grain, i.e., 300–500 µm. Folic acid and vitamin B12 were added either in the iodate spray solution or in the solid iron premix. The premixes and salt were stored at 25, 35 and 45°C at 65% RH for up to a year. The loss of iodine, folic acid and vitamin B12 were monitored. An optimized formulation was tested on the pilot scale at JVS Foods Pvt, Jaipur, India. Results Folic acid can be stabilized in the iodine spray solution, and triple fortified salt containing iodine, folic acid and encapsulated ferrous fumarate retained >90% of both the added iodine and folic acid for 6 months. Stable QFS was made by incorporating vitamin B12 in the solid iron premix at a 1:200 ratio. The process was scaled up to produce some 25 kg of iron and B12premix, sufficient for 5 tons of salt, or 500,000 daily doses of salt. Organoleptic testing of Indian meals produced with quadruple fortified salt were found to be acceptable by a consumer panel at the University of Delhi. Conclusions Stable quadruple fortified salt that can provide up to 50% of RDA of folic acid and iron and 100 + % of RDA of vitamin B12 and iodine has been developed. The product was pilot tested and had high consumer acceptability. The formulation could reduce the incidence of maternal and infant mortality at a cost of less than 20¢/annum. Funding Sources This research was funded by Grand Challenges Canada through the Saving Lives at Birth program, and by the Bill and Melinda Gates Foundation.

scholarly journals High Serum Vitamin B12 Levels Associated with C‐Reactive Protein in Older Patients with Cancer

2020 ◽  
Vol 25 (12) ◽  
Author(s):  
Anne‐Laure Couderc ◽  
Eddy Puchades ◽  
Patrick Villani ◽  
Robin Arcani ◽  
Laure Farnault ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Older Patients ◽  
Serum Vitamin ◽  
High Serum ◽  
C Reactive Protein ◽  
Patients With Cancer ◽  
Reactive Protein

scholarly journals Is Inflammation Status in Postbariatric Patients Predictive for Their Response to Vitamin B12 Supplementation Therapy?

2021 ◽  
Vol 105 (1-3) ◽  
pp. 397-401
Author(s):  
Sjaak Pouwels ◽  
Hendrika J.M. Smelt ◽  
Johannes F. Smulders
Keyword(s):  
Bariatric Surgery ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Reactive Protein ◽  
Inflammatory Status ◽  
B12 Deficiency ◽  
Injection Regimen ◽  
Vitamin B12 Supplementation

Background Several studies indicate that there is a relationship between vitamin B12 levels and inflammatory status. Some studies showed a significantly correlation between vitamin B12 status and inflammation. The aim of this study is to investigate the influence of inflammatory status on the effect of different vitamin B12 supplementation regimes. Methods We selected patients with a vitamin B12 deficiency based on methylmalonic acid (MMA) levels. A moderate vitamin B12 deficiency was defined as an MMA blood level between ≥300 and 430 nmol/L. In included patients, C-reactive protein (CRP), leukocytes, serum vitamin B12, and MMA levels were measured at baseline and after 6 months of follow-up. Results A total of 63 patients were included, treated with 3, 6, or no intramuscular vitamin B12 injections. In the 6 intramuscular injections group, the presupplementation CRP levels significantly predicted the response in terms of vitamin B12 increase (P = 0.015). Also, there was a significant reduction in CRP levels (P = 0.03) after 6 injections. There was a significant correlation between presupplementation MMA and presupplementation CRP (r = 0.127, P = 0.049). Conclusion This study showed that presupplementation CRP levels significantly predicted the response on 6 intramuscular vitamin B12 injections in patients after bariatric surgery. Second, the 6 intramuscular injection regimen showed a significant reduction in CRP levels. Third, there was a significant correlation between MMA and presupplementation CRP. This might indicate that there is interplay between the vitamin B12 supplementation and inflammatory levels in patients after bariatric surgery.

Use of soluble glass boluses containing Cu, Co and Se in the prevention of trace-element deficiencies in goats

1988 ◽  
Vol 110 (1) ◽  
pp. 155-158 ◽  
Author(s):  
G. P. Zervas
Keyword(s):  
Trace Elements ◽  
Vitamin B12 ◽  
Trace Element ◽  
Serum Vitamin ◽  
Experimental Period ◽  
The Other ◽  
Blood Samples ◽  
The Third ◽  
Soluble Glass ◽  
Plasma Copper

SummaryTwo experiments were carried out to examine the value of copper, cobalt and selenium contained in soluble glass boluses as a means of providing supplementary trace elements to goats.In the first experiment 36 purebred Toggenburg goats were used and divided into two groups. One group was treated with soluble glass boluses while the other was left as control.In the second experiment 46 Saanen half-bred goats were used, divided into three groups. The first group was treated with soluble glass boluses, the second was treated with selenium injectable compound and the third was left as control.Blood samples were collected on four occasions, during an experimental period of almost 12 months and plasma copper, serum vitamin B12 and blood selenium concentrations were determined. All those measurements showed significant increases for about a year, and were due to treatment. The advantages of glass boluses were not shared by the selenium injectable compound.

scholarly journals Holo-Transcobalamin Concentration and Transcobalamin Saturation Reflect Recent Vitamin B12 Absorption Better than Does Serum Vitamin B12

2004 ◽  
Vol 50 (6) ◽  
pp. 1043-1049 ◽  
Author(s):  
Mustafa Vakur Bor ◽  
Ebba Nexø ◽  
Anne-Mette Hvas
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Healthy Individuals ◽  
Active Vitamin ◽  
Intraindividual Variation ◽  
Blood Samples ◽  
Normal Vitamin ◽  
Vitamin B12 Absorption ◽  
Age Range ◽  
Better Than

Abstract Background: We evaluated whether measurement of vitamin B12-saturated transcobalamin (holo-TC) concentrations or TC saturation (holo-TC:total TC) reflects active vitamin B12 absorption in healthy individuals and patients after vitamin B12 intake. Methods: We obtained blood samples from 31 healthy individuals (age range, 25–57 years) before (days −1 and 0) and after (days 1, 2, and 6) oral administration of three 9-μg doses of vitamin B12. The blood samples from seven patients (age range, 22–39 years) suspected to have decreased vitamin B12 absorption were obtained before and 1 day after the vitamin B12 intake. The blood samples were analyzed for vitamin B12, total TC, and holo-TC. The TC saturation was calculated. Results: Intraindividual variation was <13% for all measured values, as calculated from samples removed on day −1 and 0. In healthy individuals (n = 31) after intake of vitamin B12, the maximum median (range) increase (as percentages and absolute values) was in TC saturation [52 (−2% to 128)% and 0.04 (0–0.23) as a fraction], closely followed by holo-TC concentrations [39 (0–108)% and 34 (0–149) pmol/L]. All but one healthy individual had an increase of ≥15% in these markers. Serum vitamin B12 showed a smaller increase [14 (−8 to 51)% and 36 (−27 to 290) pmol/L]. After vitamin B12 intake, three patients with Crohn disease had the lowest increases in holo-TC concentration (3, 7, and 14 pmol/L) and in TC saturation (0.004, 0.01, and 0.01) among patients and 30 healthy individuals. Conclusion: Holo-TC concentrations and TC saturation reflect normal vitamin B12 absorption better than does serum vitamin B12.

scholarly journals Anthropometry and C-reactive Protein and Clinical Outcomes in Children with Cancer in Intensive Care Unit (P12-023-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Wanelia Afonso ◽  
Wilza Peres ◽  
Nivaldo Pinho ◽  
Renata Martucci ◽  
Carolina Soares ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Clinical Outcomes ◽  
Body Mass ◽  
Nutritional Assessment ◽  
Treatment Center ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Funding Sources

Abstract Objectives To evaluate the influence of nutritional status on admission, according to the anthropometric indicator (Body Mass Index- BMI), serum C-reactive protein (CRP) and albumin on length of hospital stay (LHS) and time of mechanical ventilation (MV) in the Pediatric Intensive Care Unit (PICU) in a Oncological Treatment Center. Methods It is a longitudinal retrospective study with all children admitted to the PICU in 2013. The comparison of medians related to LHS and VM was assessed using the nonparametric Mann-Whitney and Kruskal Wallis test. The Kaplan-Meier curve evaluated the influence of body mass index (BMI), CRP and albumin on LHS and VM. The significance value was P < 0.05. Results A total of 54 patients were selected, median age of 8.02 (2.35–12.79) years. Solid tumors were the most common (92.6%; n = 50), and the most frequent causes of hospitalization in the PICU were respiratory failure (26.4%; n = 14) and sepsis (24.5%; n = 13). The median LHS was 11 (6–18) days and MV median was 11 (6–16.86) days. The frequency of BMI above and below the adequate level was 23.5% (n = 12) and 29.4% (n = 15), respectively. Conclusions BMI and CRP used for nutritional assessment were not associated with clinical outcomes LHS and MV in PICU. Funding Sources No funding sources.

scholarly journals POS1398 RHEUMATOLOGICAL PATIENTS PERFORMING BLOOD SELF-SAMPLING FOR DMARD THERAPY SAFETY: A PROOF OF CONCEPT STUDY COMPARING WITH VENOUS BLOOD SAMPLES

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 982.1-982
Author(s):  
S. A. Just ◽  
P. Toftegaard ◽  
U. Jakobsen ◽  
T. R. Larsen
Keyword(s):  
Venous Blood ◽  
Automated Analysis ◽  
Blood Sampling ◽  
Differential Count ◽  
Blood Samples ◽  
Reactive Protein ◽  
Dmard Treatment ◽  
Patient Reported ◽  
Venous Sample ◽  
Treatment Safety

Background:Regular blood sampling is a requirement for rheumatological patients receiving csDMARD, bDMARD or tsDMARD therapies (1). The frequent blood sampling affects the patient’s life as they use a substantial amount of time at hospitals or by the general practitioner. Often visits are time-consuming with transport, waiting time, and for some patient’s costly long travels. Giving patients the option of taking the blood samples themself in their own home, as part of a patient-centred monitoring approach, could provide the patient much higher degree of independence. Further, it may increase the quality of life, cause higher compliance with taking the control samples and possibly reduce health care costs.Objectives:1. To investigate if rheumatological patients can take capillary blood samples and describe patient-reported outcomes (PRO) about the procedure. 2. Compare the venous and capillary samples’ results. 3. Test if the laboratory automated analysis equipment can handle the small capillary samples.Methods:21 rheumatological patients, underwent capillary and venous blood sampling at up to 4 occasions (1-2 months between). Instructions were available on a pictogram. PRO data were assessed by questionnaires. The patient performed blood extraction to the capillary samples from a finger after using a device making a small incision (2 mm depth and 3 mm width). Two capillary tubes (one Microtainer K2-EDTA and one Microtainer lithium heparin with gel) were filled with a total volume of approximately 1.0 mL blood. A phlebotomist took the venous sample. Blood samples were analyzed for alanine aminotransferase (ALAT), albumin, alkaline phosphatase (ALP), calcium, C-reactive protein (CRP), creatinine, potassium, lactate dehydrogenase (LDH), urate, hemolysis index, erythrocyte corpuscular volume (MCV), haemoglobin, leukocytes, differential count and platelets.Results:A total of 53 paired capillary (C) and venous (V) samples were taken. The average perceived pain of the procedure of C sampling was VAS: 10.3 (SD:14.4) (0-100) versus V sampling VAS: 8.5 (SD:11.7). 90% of patients would accept it as a future form of blood sampling.Differences in blood samples (C versus V) were: CRP (-3.4%); Hemoglobin (-1.4%); Creatinine (-4.4%), ALAT (-2.9%), neutrophils (1.43%), platelets (-16.9%).The index of hemolysis was on average 128.9 mg/dL (SD: 203) in C versus 6.7 mg/dL (SD: 4.6) in V. Results was evaluated by a rheumatologist, and 92.5% of capillary samples could be used to evaluate the safety of DMARD treatment based on the most critical samples for this: ALAT, creatinine, neutrophils and platelets (1). The 7.5 % not accepted were all due to aggregated platelets leading to low platelet count. There was hemolysis in 18% of the samples, but the analysis results could be used despite this.Conclusion:In the majority of rheumatological patients, capillary self-sampling is well tolerated.We show that it is possible to extract the needed results from the capillary samples to evaluate DMARD treatment safety, despite higher hemolysis index. Using capillary samples taken at home could be a central instrument in future rheumatological patient-centred monitoring.References:[1]Rigby WFC et al. Review of Routine Laboratory Monitoring for Patients with Rheumatoid Arthritis Receiving Biologic or Nonbiologic DMARDs. Int J Rheumatol. 2017Disclosure of Interests:None declared

scholarly journals AB0915 BONE MINERAL DENSITY AND FRACTURE FREQUENCY IN PREMENOPAUSAL WOMEN WITH RHEUMATIC DISEASES

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1759.2-1759
Author(s):  
N. Toroptsova ◽  
O. Dobrovolskaya ◽  
O. Nikitinskaya ◽  
N. Demin ◽  
A. Smirnov ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Vitamin D ◽  
Lumbar Spine ◽  
Serum Vitamin ◽  
Premenopausal Women ◽  
Healthy Controls ◽  
C Reactive Protein ◽  
Mineral Density ◽  
Serum Vitamin D ◽  
Reactive Protein

Background:The onset of the disease in young and middle age is typical for rheumatic diseases (RDS), but most studies on osteoporosis were conducted in patients (pts) older than 50 years, which included postmenopausal women.Objectives:To assess bone mineral density (BMD), fracture frequency and the factors associated with low BMD in premenopausal women with RDs.Methods:160 women (median age, 36 [29; 43] years): 120 pts with RDs (43 rheumatoid arthritis (RA), 53 systemic sclerosis (SSc) and 24 psoriatic arthritis (PsA)) and 40 age-matched healthy controls were enrolled in the study. We performed a dual-energy X-ray absorptiometry (DXA, Hologic Discovery A, USA) to measure BMD in lumbar spine, femoral neck and total hip. BMD decreasing grade was evaluated by the Z-score <-2SD. All pts were interviewed using a unified questionnaire including assessment of daily dietary calcium intake. Serum vitamin D, C-reactive protein and erythrocyte sedimentation rate (ESR) measurements were done.Results:25% pts with RDs and only 8% healthy controls have low BMD (p=0.02). RA, SSc and PsA pts had low BMD in 37%, 21% and 13%, respectively, that was more often than in healthy women (p=0.004, p=0.046 and p= 0.081, respectively). 9,3% RA pts and 7,5% SSc pts had low energy fractures. BMD of RDs pts in all areas of measurement demonstrated a direct correlation with height, weight, body mass index, and serum vitamin D concentration and an inverse correlation with the cumulative dose of glucocorticoids. Also, proximal femur BMD inversely correlated with RDs duration. BMD of femoral neck and total hip inversely correlated with C-reactive protein level in SSc pts. In RA women we found a direct correlation between lumbar spine and femur neck BMD and ESR.Conclusion:25% of premenopausal women with RDs had reduced BMD and needed monitoring and osteoporosis prevention, while 9.3% pts with RA and 7.5% women with SSc needed anti-osteoporotic treatment.Disclosure of Interests:None declared

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