scholarly journals A new locus inCytophaga hutchinsoniiinvolved in colony spreading on agar surfaces and individual cell gliding

2015 ◽  
Vol 362 (14) ◽  
pp. fnv095 ◽  
Author(s):  
Zhe Li ◽  
Cong Zhang ◽  
Sen Wang ◽  
Jing Cao ◽  
Weican Zhang ◽  
...  
Keyword(s):  
Individual Cell ◽  
Colony Spreading

Ultrastructure of myoepithelial cell in parotid gland

1988 ◽  
Vol 46 ◽  
pp. 342-343
Author(s):  
C. N. Sun
Keyword(s):  
Parotid Gland ◽  
Osmium Tetroxide ◽  
Individual Cell ◽  
Branching Processes ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Uranyl Acetate ◽  
Thin Sections ◽  
Gland Carcinoma ◽  
Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Shaping and Cellular Uptake of Folic Acid Coated Gold and Magnetite Nanoparticles

2019 ◽  
Vol 9 (2) ◽  
pp. 166-172
Author(s):  
Ahmed A.G. El-Shahawy ◽  
Gamal Elghnam ◽  
Alsayed A.M. Alsherbini
Keyword(s):  
Folic Acid ◽  
Iron Oxide ◽  
Tumor Cells ◽  
Iron Oxide Nanoparticles ◽  
Individual Cell ◽  
Oxide Nanoparticles ◽  
Cellular Compartment ◽  
Specific Tumor ◽  
Uv Visible

Background:Gold and Iron Oxide nanoparticles NPs play as nanocarriers for a specific drug delivery and contrast agents. Intercellular uptake of these nanoparticles and targeting to individual cell and sub-cellular compartment is essential.Objective:The aim of the current study is to evaluate the intracellular uptake of these NPs to specific tumor cells in vitro conjugated with folic acid with a goal of enhancing the efficiency of specific targeting to tumor cells.Methods:We synthesized the nanoparticles by a chemical method and characterized by UV-Visible, FTIR, XRD, and TEM.Results & Conclusion:The results revealed the conjugation of Gold and Iron Oxide nanoparticles with folic acid increased the intercellular uptake with high percent compared to non- conjugated nanoparticles.

Managing globalization of R&D: Organizing for offshoring innovation

2006 ◽  
Vol 25 (2) ◽  
pp. 103-114
Author(s):  
Ashok Deo Bardhan
Keyword(s):  
Management Practices ◽  
Individual Cell ◽  
Organization Structure ◽  
Innovative Activity ◽  
Basic Building Block ◽  
Market Incentives ◽  
The World ◽  
Foreign Outsourcing ◽  
Input Markets ◽  
High Skill

This article analyzes the challenges brought about by the globalization of innovative activity to the science and practice of management. The task of matching organization structure and management practices to the needs of R&D offshoring is analyzed through a set of dichotomous pairs of concepts: (1) Drastic vs. Gradual and Systemic vs. Autonomous Innovation, (2) High vs. Low Skill Specificity, (3) Input Markets vs. Output Markets, (4) Intra-Firm vs. Arms Length Offshoring. In the trade-off between markets and hierarchies, the firms often come down on the side of the latter when it comes to the setting up of R&D facilities abroad. Organizational directives and internalization, i.e., intra-firm offshoring can trump market incentives and foreign outsourcing, when it comes to the uncertain returns from innovative activity, particularly in the case of drastic innovations and high skill specificity. Globalization has led to dispersed markets and firms have responded with dispersed locations of core assets, creating competence clusters all over the world, and the innovative firm of the future will restructure each individual cell, the basic building block of the firm consisting of an occupation devoted to a product, and redeploy and relocate them globally, where it is most advantageous.

scholarly journals Colony spreading of the gliding bacterium Flavobacterium johnsoniae in the absence of the motility adhesin SprB

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Keiko Sato ◽  
Masami Naya ◽  
Yuri Hatano ◽  
Yoshio Kondo ◽  
Mari Sato ◽  
...  
Keyword(s):  
Soft Agar ◽  
Gliding Motility ◽  
Wild Type ◽  
Initial Cell ◽  
Flavobacterium Johnsoniae ◽  
Seeking Behavior ◽  
Colony Spreading ◽  
Gliding Bacterium ◽  
Mutant Cells ◽  
Scanning Electron

AbstractColony spreading of Flavobacterium johnsoniae is shown to include gliding motility using the cell surface adhesin SprB, and is drastically affected by agar and glucose concentrations. Wild-type (WT) and ΔsprB mutant cells formed nonspreading colonies on soft agar, but spreading dendritic colonies on soft agar containing glucose. In the presence of glucose, an initial cell growth-dependent phase was followed by a secondary SprB-independent, gliding motility-dependent phase. The branching pattern of a ΔsprB colony was less complex than the pattern formed by the WT. Mesoscopic and microstructural information was obtained by atmospheric scanning electron microscopy (ASEM) and transmission EM, respectively. In the growth-dependent phase of WT colonies, dendritic tips spread rapidly by the movement of individual cells. In the following SprB-independent phase, leading tips were extended outwards by the movement of dynamic windmill-like rolling centers, and the lipoproteins were expressed more abundantly. Dark spots in WT cells during the growth-dependent spreading phase were not observed in the SprB-independent phase. Various mutations showed that the lipoproteins and the motility machinery were necessary for SprB-independent spreading. Overall, SprB-independent colony spreading is influenced by the lipoproteins, some of which are involved in the gliding machinery, and medium conditions, which together determine the nutrient-seeking behavior.

Modelling the individual cell lag phase: effect of temperature and pH on the individual cell lag distribution of

2005 ◽  
Vol 100 (1-3) ◽  
pp. 41-53 ◽  
Author(s):  
K FRANCOIS ◽  
F DEVLIEGHERE ◽  
K SMET ◽  
A STANDAERT ◽  
A GEERAERD ◽  
...  
Keyword(s):  
Individual Cell ◽  
Effect Of Temperature ◽  
Lag Phase ◽  
Phase Effect ◽  
The Individual

scholarly journals Cancer Cell Invasion of Brain Tissue: Guided by a Prepattern?

2005 ◽  
Vol 6 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Michael Wurzel ◽  
Carlo Schaller ◽  
Matthias Simon ◽  
Andreas Deutsch
Keyword(s):  
Lattice Gas ◽  
Individual Cell ◽  
Physical Structure ◽  
Tumour Cells ◽  
Malignant Cells ◽  
Malignant Brain Tumour ◽  
Tumour Bulk ◽  
Cell Motion ◽  
The Brain ◽  
Global Growth

The malignant brain tumourGlioblastoma multiforme(GBM) displays a highly invasive behaviour. Spreading of the malignant cells appears to be guided by the white matter fibre tracts within the brain. In order to understand the global growth process we introduce a lattice-gas cellular automaton model which describes the local interaction between individual malignant cells and their neighbourhood. We consider interactions between cells (brain cells and tumour cells) and between malignant cells and the fibre tracts in the brain, which are considered as a prepattern. The prepattern implies persistent individual cell motion along the fibre structure. Simulations with the model show that only the inclusion of the prepattern results in invading tumour and growing tumour islets in front of the expanding tumour bulk (i.e. the growth pattern observed in clinical practice). Our results imply that the infiltrative growth of GBMs is, in part, determined by the physical structure of the surrounding brain rather than by intrinsic properties of the tumour cells.

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