scholarly journals Application of RNA subcellular fraction estimation method to explore RNA localization regulation

2021 ◽  
Author(s):  
Xiaomin Dai ◽  
Yangmengjie Li ◽  
Weizhen Liu ◽  
Xiuqi Pan ◽  
Chenyue Guo ◽  
...  
Keyword(s):  
Subcellular Fraction ◽  
Estimation Method ◽  
Multiple Linear Regression Model ◽  
Subcellular Fractionation ◽  
Rna Localization ◽  
Global Scale ◽  
Cellular Compartment ◽  
Localization Pattern ◽  
Relative Localization ◽  
The Difference

Abstract RNA localization is involved in multiple biological processes. Recent advances in subcellular fractionation-based sequencing approaches uncovered localization pattern on a global scale. Most of existing methods adopt relative localization ratios (such as ratios of separately normalized transcripts per millions of different subcellular fractions without considering the difference in total RNA abundances in different fractions), however, absolute ratios may yield different results on the preference to different cellular compartment. Experimentally, adding external Spike-in RNAs to different fractionation can be used to obtain absolute ratios. In addition, a spike-in independent computational approach based on multiple linear regression model can also be used. However, currently, no custom tool is available. To solve this problem, we developed a method called subcellular fraction abundance estimator to correctly estimate relative RNA abundances of different subcellular fractionations. The ratios estimated by our method were consistent with existing reports. By applying the estimated ratios for different fractions, we explored the RNA localization pattern in cell lines and also predicted RBP motifs that were associated with different localization patterns. In addition, we showed that different isoforms of same genes could exhibit distinct localization patterns. To conclude, we believed our tool will facilitate future subcellular fractionation-related sequencing study to explore the function of RNA localization in various biological problems.

On the Role of Transferrin in 67Ga Uptake by Tumor Cells

1988 ◽  
Vol 27 (04) ◽  
pp. 151-153
Author(s):  
P. Thouvenot ◽  
F. Brunotte ◽  
J. Robert ◽  
L. J. Anghileri
Keyword(s):  
Specific Binding ◽  
Subcellular Fractionation ◽  
Animal Blood ◽  
Tumor Bearing ◽  
Cell Structures ◽  
The Difference ◽  
Sarcoma Cells ◽  
In Vitro Uptake

In vitro uptake of 67Ga-citrate and 59Fe-citrate by DS sarcoma cells in the presence of tumor-bearing animal blood plasma showed a dramatic inhibition of both 67Ga and 59Fe uptakes: about ii/io of 67Ga and 1/5o of the 59Fe are taken up by the cells. Subcellular fractionation appears to indicate no specific binding to cell structures, and the difference of binding seems to be related to the transferrin chelation and transmembrane transport differences

scholarly journals Refinement of the ice absorption spectrum in the visible using radiance profile measurements in Antarctic snow

2016 ◽  
Author(s):  
Ghislain Picard ◽  
Quentin Libois ◽  
Laurent Arnaud
Keyword(s):  
Absorption Coefficient ◽  
Fiber Optics ◽  
Estimation Method ◽  
Radiative Transfer Model ◽  
Transfer Model ◽  
Snow Layer ◽  
Antarctic Snow ◽  
Order Of Magnitude ◽  
The Difference ◽  
The Antarctic

Abstract. Ice is a highly transparent material in the visible. According to the most widely used database (Warren and Brandt, 2008; IA2008), the ice absorption coefficient reaches values lower than 10−3 m−1 around 400 nm. These values were obtained from a radiance profile measured in a single snow layer at Dome C in Antarctica. We reproduced this experiment using a fiber optics inserted in the snow to record 56 profiles from which 70 homogeneous layers were identified. Applying the same estimation method on every layer yields 70 ice absorption spectra with a significant variability and overall larger than IA2008 by one order of magnitude. We devise another estimation method based on Bayesian inference. It reduces the statistical variability and confirms the higher absorption, around 2 × 10−2 m−1 near the minimum at 440 nm. We explore potential instrumental artifacts by developing a 3D radiative transfer model able to explicitly account for the presence of the fiber in the snow. The simulation results show that the radiance profile is indeed perturbed by the fiber intrusion but the error on the ice absorption estimate is not larger than a factor 2. This is insufficient to explain the difference between our new estimate and IA2008. Nevertheless, considering the number of profiles acquired for this study and other estimates from the Antarctic Muon and Neutrino Detector Array (AMANDA), we estimate that ice absorption values around 10−2 m−1 at the minimum are more likely than under 10−3 m−1. We provide a new estimate in the range 400–600 nm for future modeling of snow, cloud, and sea-ice optical properties. Most importantly we recommend that modeling studies take into account the large uncertainty of the ice absorption coefficient in the visible.

Urban heat island estimation from improved selection of urban and rural stations by DTW algorithm

2021 ◽  
Author(s):  
Yonghong Hu ◽  
Gensuo Jia ◽  
Jinlong Ai ◽  
Yong Zhang ◽  
Meiting Hou ◽  
...  
Keyword(s):  
Urban Heat Island ◽  
Urban Climate ◽  
Global Scale ◽  
Heat Island ◽  
Climate Effect ◽  
Urban Heat ◽  
The Difference ◽  
Temperature Records ◽  
Dynamic Time ◽  
Selection Of

Abstract Typical urban and rural temperature records are essential for the estimation and comparison of urban heat island effects in different regions, and the key issues are how to identify the typical urban and rural stations. This study tried to analyze the similarity of air temperature sequences by using dynamic time warping algorithm (DTW) to improve the selection of typical stations. We examined the similarity of temperature sequences of 20 stations in Beijing and validated by remote sensing, and the results indicated that DTW algorithm could identify the difference of temperature sequence, and clearly divide them into different groups according to their probability distribution information. The analysis for station pairs with high similarity could provide appropriate classification for typical urban stations (FT, SY, HD, TZ, CY, CP, MTG, BJ, SJS, DX, FS) and typical rural stations (ZT, SDZ, XYL) in Beijing. We also found that some traditional rural stations can’t represent temperature variation in rural surface because of their surrounding environments highly modified by urbanization process in last decades, and they may underestimate the urban climate effect by 1.24℃. DTW algorithm is simple in analysis and application for temperature sequences, and has good potentials in improving urban heat island estimation in regional or global scale by selecting more appropriate temperature records.

DETERMINATION OF BOTANICAL COMPOSITION OF TWO-COMPONENT FORAGE MIXTURES

1960 ◽  
Vol 40 (2) ◽  
pp. 225-234 ◽  
Author(s):  
J. W. Tanner ◽  
E. E. Gamble ◽  
W. E. Tossell
Keyword(s):  
Unit Cost ◽  
Estimation Method ◽  
Separation Method ◽  
Botanical Composition ◽  
Visual Estimation ◽  
Two Component ◽  
Late Maturity ◽  
The Difference ◽  
Forage Mixtures

A comparative study was made in 1958 of the visual estimation and hand separation methods of determining botanical composition of two-component forage mixtures. The results indicated that there were positive significant correlations between the per cent legume values obtained by the two methods. The visual estimation method was less variable than the hand separation method and the precision per unit cost was greater. The differences between per cent legume values obtained by the two methods were influenced by the stage of maturity (medium or late hay) of the components and the cut (hay or aftermath). In this study, the difference was significant only in the medium aftermath cut.Individually, three observers showed some inconsistencies between estimates on the medium and late maturity groups and between the hay and aftermath cut. However, by averaging the three estimates to obtain a mean sample, these inconsistencies were minimized.Both methods were more precise in the aftermath pasture cut than in the hay. An additional observer increased precision of the visual estimate more than an additional replicate or sample. The greater precision resulting from additional replicates, samples, or observers increased at a decreasing rate. The number of replicates, samples, and observers required for specific degrees of precision and a specific cost were calculated.The experiment showed that the visual estimation method can be superior to the hand separation method as a means of determining botanical composition.

scholarly journals Comparative Study of Comfort Indicators for School Constructions in Sustainability Methodologies: Schools in the Amazon and the Southeast Region of Brazil

2019 ◽  
Vol 11 (19) ◽  
pp. 5216 ◽  
Author(s):  
Tatiana Santos Saraiva ◽  
Edson Martins da Silva ◽  
Manuela Almeida ◽  
Luís Bragança
Keyword(s):  
Social Issues ◽  
Global Scale ◽  
Northern Region ◽  
Sustainable Construction ◽  
School Buildings ◽  
Environmental Preservation ◽  
Southeast Region ◽  
Acoustic Comfort ◽  
The Difference ◽  
Entire Planet

In the 1970s and 1980s, the effects of pollution in the atmosphere grew from a local to a global scale, affecting the entire planet. Consequently, economic and social issues began to be increasingly more connected with environmental protection. Several measures were taken towards environmental preservation, many of those related to the sustainable construction of buildings. This work intended to make a parallel between comfort indicators among different schools in Brazil, beginning with the specific analyses of schools in Juiz de Fora (Minas Gerais, MG), in the Southeast region, and in Macapá (Amapa, AP), in the Amazon or Northern region. This comparison between schools is made to demonstrate the need to adapt methodologies and certifications that promote sustainability in school buildings for each region of Brazil. Questionnaires about ergonomic, thermal, visual and acoustic comfort, and air quality, were applied in two high-school buildings in Juiz de Fora, Academia School and Santa Catarina School, and in two high schools in Macapá, Tiradentes School and Professor Gabriel Almeida Café school, to understand the difference between these two regions of Brazil regarding comfort conditions. A comparison between the results of the four schools was made, proving the necessity of the elaboration of a specific methodology for each Brazilian region.

scholarly journals Estimation accuracy of covariance matrices when their eigenvalues are almost duplicated

2018 ◽  
Vol 7 ◽  
Author(s):  
Kantaro Shimomura ◽  
Kazushi Ikeda
Keyword(s):  
Covariance Matrix ◽  
Estimation Method ◽  
Estimation Accuracy ◽  
Essential Information ◽  
The Matrix ◽  
The Difference ◽  
Processing Techniques ◽  
Monotonic Property ◽  
Signal Processing Techniques ◽  
Special Case

The covariance matrix of signals is one of the most essential information in multivariate analysis and other signal processing techniques. The estimation accuracy of a covariance matrix is degraded when some eigenvalues of the matrix are almost duplicated. Although the degradation is theoretically analyzed in the asymptotic case of infinite variables and observations, the degradation in finite cases are still open. This paper tackles the problem using the Bayesian approach, where the learning coefficient represents the generalization error. The learning coefficient is derived in a special case, i.e., the covariance matrix is spiked (all eigenvalues take the same value except one) and a shrinkage estimation method is employed. Our theoretical analysis shows a non-monotonic property that the learning coefficient increases as the difference of eigenvalues increases until a critical point and then decreases from the point and converged to the distinct case. The result is validated by numerical experiments.

The new gravity system—changes in international gravity base values and anomaly values

Geophysics ◽  
1979 ◽  
Vol 44 (8) ◽  
pp. 1352-1366 ◽  
Author(s):  
George P. Woollard
Keyword(s):  
Sea Level ◽  
Global Scale ◽  
Absolute Gravity ◽  
Geodetic Reference System ◽  
Limiting Factor ◽  
Worldwide Distribution ◽  
The Difference ◽  
Correction Terms ◽  
Discernible Difference ◽  
Gravity System

An assessment is made of (1) the reliability of the changes in the absolute gravity datum and standard incorporated in the IGSN 71 values of Morelli et al (1974), and (2) the effect of these changes and those incorporated in the new international gravity formula (Geodetic Reference System 1967) defining theoretical gravity values at sea level on gravity anomaly values. Pendulum‐interval comparisons with modern absolute gravity intervals between Washington, D.C.; Teddington, England; Paris, France; and Potsdam, East Germany indicate that the correction of 14 mgal to the Potsdam datum is correct to within .03 ± .016 mgal. Although there appear to be occasional discrepancies in the IGSN 71 values of the order of 0.1 mgal, in general their reliability appears to approximate ±0.05 mgal. A discrepancy of approximately −0.03 mgal per 1000 mgal of absolute gravity is indicated, however, in the gravity standard defined by the IGSN 71 values over the range of modern absolute gravity determinations between Fairbanks, Alaska and Bogotá, Colombia. An evaluation of the earlier attempt to standardize gravity on a global scale (Woollard and Rose, 1963) indicates a mean datum difference of 14.7 mgal (standard deviation 0.2 mgal) relative to IGSN 71 values at 776 sites having a worldwide distribution. No discernible difference in gravity standard from that of the IGSN 71 values is indicated except in South America, where the Woollard and Rose values are found to incorporate a difference in standard of O.2 mgal per 1000 mgal. It is shown that anomaly conversions from the old to the new gravity system can be effected directly using the old anomaly values. Two correction terms are required, specifically, (1) a constant representing the difference in base value used relative to the IGSN 71 value for the base, and (2) a latitude‐dependent term representing the difference in theoretical sea level gravity using the old International Gravity Formula (IGF) and the new GRS 67 formula. Test conversions of anomaly values on the old gravity system for regional areas involving data from several sources indicate that, in general, anomaly conversion to the new gravity system can be obtained with a reliability of about 0.3 to 0.5 mgal. The limiting factor is the unknown variation in gravimeter calibration standards represented.

Quantification of Concrete Waste Generated in Housing Construction Sites via Site Sampling: Development of a Multiple Linear Regression Model

2015 ◽  
Vol 802 ◽  
pp. 676-681
Author(s):  
Siti Hafizan Hassan ◽  
Hamidi Abdul Aziz ◽  
Izwan Johari ◽  
Mohd Nordin Adlan
Keyword(s):  
Linear Regression ◽  
Regression Model ◽  
Multiple Linear Regression ◽  
Linear Regression Analysis ◽  
Estimation Method ◽  
Multiple Linear Regression Model ◽  
Real Data ◽  
Housing Construction ◽  
Construction Sites ◽  
Average Percentage

Waste generated in construction sites has recently increased and has become an uncontrollable cause of environmental problems and profit loss to contractors. The lack of real data or research on such wastes is due to the lack of suitable policies regarding this issue. The actions of contractors are not controlled by rules on this issue. This situation leads to the lack of action or awareness on the side of the contractor. Concrete waste is also part of the waste generated in construction sites. We determine the concrete waste generated in construction stages and conduct multiple linear regression analysis of the amount of column waste generated. The methodology employed in this study involves site observations, interviews with site personnel, and sampling at housing construction sites. The estimation method is utilized for the sampling of concrete waste. Results show that the average percentage of column waste is 13.93% and that of slab waste is 0.34%. These percentage values are derived from the total order of the concrete. The difference is due to the sizes of structures and method of handling. The regression model obtained from the sample data on column waste resulted in an adjustedR2value of 0.895. Therefore, the model predicts approximately 89.5% of the factors involved in concrete waste generation.

A Novel Rotor Temperature Estimation Method Base on Dq-Axis Flux Linkage Observation

2015 ◽  
Vol 723 ◽  
pp. 979-982
Author(s):  
Yan Ling Cao ◽  
Ping Cao
Keyword(s):  
Permanent Magnet ◽  
Electric Motor ◽  
Estimation Method ◽  
Temperature Characteristic ◽  
Test Results ◽  
Flux Linkage ◽  
Temperature Estimation ◽  
Method Base ◽  
The Difference

This paper presents a method for determining the rotor temperature of an electric motor by observing the dq-axis flux linkage. A reference dq-axis flux linkage is obtained at a defined rotor temperature. The difference between calculated and reference flux in the d-axis is supposed to be caused by temperature, therefore the temperature can be back-calculated according to the permanent magnet temperature characteristic. The proposed method was confirmed to be correct and effective by the test results.

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