166 Optimal tryptophan:lysine Ratio for 25–40 Kg Growing Pigs Fed Diets Containing 35 % Distillers Dried Grains with Solubles

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 57-58
Author(s):  
Maryane S Sespere Faria Oliveira ◽  
John K Htoo ◽  
J Caroline González-Vega ◽  
Hans H Stein
Keyword(s):  
Basal Diet ◽  
Growing Pigs ◽  
High Concentration ◽  
Final Body Weight ◽  
Plasma Urea ◽  
Blood Samples ◽  
Distillers Dried Grains ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Dietary Treatments

Abstract Distillers dried grains with solubles (DDGS) has a high concentration of Leu, and the Trp requirement for growing pigs may be increased if diets contain excess Leu. Therefore, the objective of this experiment was to determine the optimum standardized ileal digestible (SID) Trp:Lys ratio in growing pigs fed diets with excess Leu from DDGS. A diet based on corn, soybean-meal, and 35% DDGS was formulated to be deficient in Trp and Lys, according to NRC requirements (0.13% SID Trp; 0.88% SID Lys; 15% SID Trp:Lys ratio). Four diets were prepared by adding L-Trp to the basal diet, which resulted in analyzed SID Trp:Lys ratios of 18, 20, 23, and 24% in these diets. One-hundred and twenty growing pigs (26.3 ± 2.0 kg) were allotted to one of the 5 dietary treatments with 3 pigs per pen and 8 pen replicates in a completely randomized design. Diets were fed for 21d and blood samples were collected on d-21. Data were analyzed by linear and quadratic contrasts in SAS. The optimal SID Trp:Lys ratio was estimated using linear broken-line (LBL) and quadratic broken-line (QBL) regressions for ADG and G:F, using NLIN procedure in SAS. Results indicated that average daily feed intake, ADG, G:F, and final body weight increased (linear and quadratic, P < 0.01) and plasma-urea-nitrogen decreased (linear and quadratic, P < 0.05) as dietary SID Trp:Lys increased (Table 1). The SID Trp:Lys ratio to optimize ADG was 20.9 and 23.4% by LBL and QBL, respectively. The G:F was optimized at 18.7 and 20.2% by LBL and QBL, respectively. The average SID Trp:Lys ratio for the 4 measurements was 20.8% which is greater than the current NRC requirement (SID 17.3%). In conclusion, diets formulated with 35% DDGS may need more dietary Trp than current NRC values.

scholarly journals Effects on nitrogen balance and metabolism of branched-chain amino acids by growing pigs of supplementing isoleucine and valine to diets with adequate or excess concentrations of dietary leucine

2020 ◽  
Vol 98 (11) ◽  
Author(s):  
Woong B Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Plasma Concentration ◽  
Plasma Concentrations ◽  
Basal Diet ◽  
Branched Chain Amino Acids ◽  
Growing Pigs ◽  
Plasma Urea ◽  
N Retention ◽  
Branched Chain ◽  
Dietary Treatments

Abstract Diets based on high levels of corn protein have elevated concentrations of Leu, which may negatively affect N retention in pigs. An experiment was, therefore, conducted to test the hypothesis that Ile and Val supplementation may overcome the detrimental effects of excess dietary Leu on N balance and metabolism of branched-chain amino acids (BCAA) in growing pigs. A total of 144 barrows (initial body weight: 28.5 kg) were housed in metabolism crates and randomly assigned to 1 of 18 dietary treatments. The basal diet contained 0.98% standardized ileal digestible (SID) Lys and had SID Leu, Val, and Ile ratios to SID Lys of 100%, 60%, and 43%, respectively. Crystalline l-Leu (0% or 2.0%), l-Ile (0%, 0.1%, or 0.2%), and l-Val (0%, 0.1%, or 0.2%) were added to the basal diet resulting in a total of 18 dietary treatments that were arranged in a 2 × 3 × 3 factorial. Urine and fecal samples were collected for 5 d after 7 d of adaptation. Blood, skeletal muscle, and liver samples were collected at the conclusion of the experiment. There were no three-way interactions among the main effects. Excess Leu in diets reduced (P < 0.05) N retention and biological value of protein and increased (P < 0.001) plasma urea N (PUN), but PUN was reduced (P < 0.05) as dietary Val increased. Concentrations of Leu in the liver were greater (P < 0.001) in pigs fed excess Leu diets than in pigs fed adequate Leu diets, but concentrations of BCAA in muscle were greater (P < 0.05) in pigs fed low-Leu diets. Increasing dietary Ile increased (P < 0.001) plasma-free Ile and plasma concentration of the Ile metabolite, α-keto-β-methylvalerate, but the increase was greater in diets without excess Leu than in diets with excess Leu (interaction, P < 0.001). Plasma concentrations of Val and the Val metabolite α-keto isovalerate increased (P < 0.001) with increasing dietary Val in diets with adequate Leu, but not in diets with excess Leu (interaction, P < 0.001). Increasing dietary Leu increased (P < 0.001) plasma-free Leu and plasma concentration of the Leu metabolite, α-keto isocaproate (KIC). In contrast, increased dietary Val reduced (P < 0.05) the plasma concentration of KIC. In conclusion, excess dietary Leu reduced N retention and increased PUN in growing pigs, but Val supplementation to excess Leu diets may increase the efficiency of amino acid utilization for protein synthesis as indicated by reduced PUN.

scholarly journals The Bioavailability Of Phosphorous From Bone Meal And Triple Super Phosphate And Its Effects On Indigenous Growing Cattle

1970 ◽  
Vol 37 (2) ◽  
pp. 58-65
Author(s):  
MM Rahman ◽  
ZH Khandaker ◽  
ABM Khaleduzzaman
Keyword(s):  
Blood Serum ◽  
Control Diet ◽  
Live Weight ◽  
Basal Diet ◽  
Bone Meal ◽  
Randomized Design ◽  
Triple Super Phosphate ◽  
Growing Cattle ◽  
Completely Randomized Design ◽  
Dietary Treatments

The bioavailability of phosphorous, and blood serum and rumen liquor P level was studied in indigenous growing cattle by feeding bone meal and Triple Super Phosphate (TSP) with control diet. Nine indigenous growing cattle (live weight of 164 ± 21 kg) were randomly assigned in a Completely Randomized Design (CRD) to three dietary treatments, consisted of a basal diet (T0) of 0.16% P and experimental diets supplemented with bone meal (T1) and triple super phosphate (T2) to provide P level of 0.44%. All diets were formulated to be iso-nitrogenous and iso-caloric. Three cannulated animals were used for rumen liquor P level study by feeding three diets. Apparent absorption and balance of P were significantly (P<0.01) higher in T1 and T2 diets than that of diet T0, but true absorption did not differ significantly (P>0.05). Bioavailability of P of control diet (37.41%), bone meal (81.45%) and triple super phosphate (80.49%) were significantly differed (P<0.01). Rumen liquor and blood serum P level differed significantly (P<0.01). Supplementation of bone meal and triple super phosphate results in high blood serum P (P>0.05) with no difference between bone meal and triple super phosphate. The results suggested that the bioavailability of P from bone meal and TSP was high and dietary supplementation of P with bone meal and TSP improved P retention and blood serum P level. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9882 BJAS 2008; 37(2): 58-65

scholarly journals PENGGUNAAN TEPUNG LIMBAH LABU KUNING DALAM RANSUM TERHADAP PENAMPILAN PRODUKSI AYAM RAS PETELUR

ZOOTEC ◽  
2016 ◽  
Vol 36 (2) ◽  
pp. 342
Author(s):  
Devis F. Komalig ◽  
Jein Rinny Leke ◽  
J. Laihat ◽  
C. Sarajar
Keyword(s):  
Laying Hens ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Laying Hen ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Dietary Treatments

THE EFFECT OF PUMPKIN WASTE MEAL IN LAYING HEN FEED ON PERFORMANS PRODUCTION. This research was to find out the use of pumpkin waste meal on performance in the production of laying hens. The purpose of this research was to observe the effect of the use of pumpkin waste meal performance in the production of feed for laying hens. A total of 100 MB 402 laying hens from 42 week of age were randomly alloted to four dietary treatments with 5 replication groups of 4 hens. Dietary treatment were; basal diet (R0), basal diet 98% + 2% WCP (R1), basal diet 96% + 4% WCP (R2), basal diet 94% + 6% WCP (R3), basal diet 92% + 8% WCP (R4). The variable were the feed Consumption, hen day production, feed conversion. Data was analyzed with completely randomized Design ( CRD ). The addition of in the feed was not influenced significantly (P > 0,05) to the feed consumption, hen day production, feed conversion. Based on the research that is done it can be concluted that the use of waste carrot pumpkins in the feed at the level of 8% show no significantly different result on the feed consumption, hen day production, feed conversion. Keyword : Pumpkin waste meal, performance laying hens, performance of production

scholarly journals The effect of various level of skipjack tuna bone meal (Katsuwonus pelamis L.) in ration on broiler carcass tenderness and abdominal fat

2011 ◽  
Vol 27 (4) ◽  
pp. 1727-1731
Author(s):  
H.A.W. Lengkey ◽  
B. Bagau ◽  
L. Adriani ◽  
M. Ludong
Keyword(s):  
Basal Diet ◽  
Abdominal Fat ◽  
Skipjack Tuna ◽  
Bone Meal ◽  
Katsuwonus Pelamis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Dietary Treatments

Hundred day old chicks Arbor Acres CP-707 were used randomizedly in this experiment, to study the effects of various levels skipjack tuna bone meal in ration on broiler carcass tenderness and abdominal fat, and were studied for six weeks. Research using Completely Randomized Design (CRD). The dietary treatments are: R0 basal diet as control, R1 basal diet + 2% tuna bone meal, R2 basal diet + 4% tuna bone meal and R3 basal diet + 6% tuna bone meal, and each treatments were repeated five times. Results indicated that the highest carcass tenderness was get from the broiler that fed basal diet with 6% tuna bone meal (125,4 mm/g/10sec) and the lowest was get from the broiler that fed basal diet (107 mm/g/10sec). For the abdominal fat, the results is broiler that adding skipjack tuna bone meal in the ration will give more abdominal fat to the broiler (1.89% - 1.92%) versus 1.85% for basal diet.

scholarly journals Effect of aflatoxin B1 on digestibility and blood parameters in horses

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Auro Braga ◽  
Fernando Queiroz de Almeida ◽  
Vinicius Pimentel Silva ◽  
Kelly Moura Keller ◽  
Marina Prado Maciel ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Adaptation Period ◽  
Blood Cell Count ◽  
Collection Method ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design

The aim of this research was to evaluate the effect of mycotoxins on the digestion of nutrients and on the hematological profile of horses. Twelve horses were used in a completely randomized design, with three treatments and four replicates. Aliquots of 50 or 100 µg kg-1 of aflatoxin B1 (AFB1) were added to a basal diet naturally containing mycotoxins. The basal diet did not contain AFB1. The 40-day evaluation included a 12-day adaptation period and a 28-day experimental period. A digestion test was carried out at the end of the experimental period, using the partial stool collection method, using LIPE® as an indicator. Blood samples were collected once a week during 4 weeks of the essay for hematological and biochemical evaluations. The amounts of mycotoxins added did not influence the intake and digestibility of the nutrients (P>0.05). The aflatoxins in the diet influenced the white blood cell count, especially mature neutrophils and the creatine kinase and alkaline phosphatase (P <0.05), which had higher activity in horses fed diets with greater toxicity.  

scholarly journals Effects of guar gum on plasma urea, insulin and glucose in the growing pig

1989 ◽  
Vol 61 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Kjell MalmlÖf ◽  
Carlos Simoes Nunes ◽  
Stefan Askbrant
Keyword(s):  
Gastrointestinal Tract ◽  
Test Meal ◽  
Guar Gum ◽  
Basal Diet ◽  
Growing Pigs ◽  
Plasma Urea ◽  
Blood Samples ◽  
Arterial Blood ◽  
Time Period ◽  
Growing Pig

1. Six growing pigs fitted with portal and arterial blood cannulas were given a barley-fishmeal diet, either alone or supplemented with guar gum at 60 g/kg basal diet. Blood samples were taken during 8 h following test meals given at 08.00 hours.2. Ingestion of the guar-gum-supplemented diet appeared to increase systematically portal and arterial levels of plasma urea. At peak values, 4 and 5 h after the test meal, this effect was statistically significant (P <0.05).3. Irrespective of which diet was given, portal and arterial blood samples, withdrawn at the same time, were found to have about the same concentration of urea. This was found throughout the 8 h studied and implies that no net exchange of urea between the circulation and the gastrointestinal tract, as a whole, took place.4. In the time-period 30–60 min following the test meal, guar gum significantly reduced the postprandial hyperglycaemia and hyperinsulinaemia in portal blood.

scholarly journals Effects of dietary supplementation of β-mannanase on performance and egg quality in laying hens

2019 ◽  
Vol 18 (03) ◽  
pp. 41-47
Author(s):  
Tung M. Che
Keyword(s):  
Moisture Content ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Productive Performance ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Dietary Treatments

The objective of the experiment was to evaluate effects of dietary supplementation of β-mannanase (Hemicell®) on productive performance, egg quality, and fecal moisture content in laying hens from 20 to 35 weeks of age. A total of 375 Isa Brown’s hens (1615.6 ± 76.4 g/bird) were randomly assigned to 5 treatments in a completely randomized design. The 5 dietary treatments included (1) basal diet with a level of 2800 kcal ME and no β-mannanase supplementation (HE, Control), (2) HE + 32 units of β-mannanase/g of feed, (3) HE + 64 units of β-mannanase/g of feed, (4) basal diet with a level of 2700 kcal ME (LE) + 32 units of β-mannanase/g of feed, and (5) LE + 64 units of β-mannanase/g of feed. Each treatment was replicated with 25 cages of 3 hens each. All diets were in meal form and contained no antibiotics. The addition of β-mannanase to HE diets did not affect the egg production of birds as compared with the control (P > 0.05). The birds fed LE diets with β-mannanase had the same egg production as those fed the control and β-mannanase-supplemented HE diets (P > 0.05). Differences in egg weight, egg quality, survival rate, and fecal moisture content were not significant among the treatments (P > 0.05). Briefly, addition of β-mannanase (32 units/g of feed) to LE diets would be beneficial for layers during the early laying period as it resulted in the same performance and egg quality as the HE diets without β-mannanase supplementation.

scholarly journals The Effect of Dietary Supplementation with Inorganic or Organic Selenium on the Nutritional Quality and Shelf Life of Goose Meat and Liver

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 261
Author(s):  
Zabihollah Nemati ◽  
Kazem Alirezalu ◽  
Maghsoud Besharati ◽  
Benjamin W. B. Holman ◽  
Mohammadreza Hajipour ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Storage Period ◽  
Thiobarbituric Acid ◽  
Basal Diet ◽  
Organic Selenium ◽  
Randomized Design ◽  
Inorganic Selenium ◽  
Completely Randomized Design ◽  
Tbars Values ◽  
Dietary Treatments

Ninety-six male goslings were allocated and assigned to treatment using a completely randomized design. Dietary treatments included a basal diet consisting of corn, wheat, and soybean meal with either no additional selenium (CON), 0.3 mg/kg of inorganic selenium (I-Se; sodium selenite), or 0.3 mg/kg of organic selenium (O-Se; selenium-enriched yeast). After a 56-day feeding period, geese were slaughtered on a common ending day and two geese per pen (n = 24) were used for the analyses conducted in this study. Meat (equal portions of the breast and thigh meat) and liver were collected and evaluated for proximate composition, fatty acid profile, pH, phenolic content, thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVB-N) over a 9-day storage period at 4 °C. The meat and liver samples from geese supplemented I-Se or O-Se had greater (p < 0.01) lipid content compared with geese not supplemented with additional selenium. At the conclusion of the 9-day storage period, meat and liver samples from geese supplemented I-Se or O-Se had lower (p < 0.05) pH values, greater (p < 0.05) phenolic content, lower (p < 0.05) TBARS values, and lower (p < 0.05) TVB-N compared with geese not supplemented with additional selenium (CON).

scholarly journals Effects of Substitution of Corn with Ground Brown Rice on Growth Performance, Nutrient Digestibility, and Gut Microbiota of Growing-Finishing Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 375
Author(s):  
Sheena Kim ◽  
Jin Ho Cho ◽  
Younghoon Kim ◽  
Hyeun Bum Kim ◽  
Minho Song
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Block Design ◽  
Brown Rice ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Finishing Pigs ◽  
Dietary Treatments

The present study was conducted to evaluate the effects of replacing corn with brown rice on growth performance, nutrient digestibility, carcass characteristics, and gut microbiota of growing and finishing pigs. A total of 100 growing pigs (23.80 ± 2.96 kg BW; 10 weeks of age) were randomly allotted to 4 dietary treatments (5 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW) as follows: corn-soybean meal basal diet (CON) and replacing corn with 50% (GBR50), 75% (GBR75), and 100% (GBR100) of ground brown rice. Each trial phase was for 6 weeks. During the growing period, there were no differences on growth performance and nutrient digestibility among dietary treatments. Similarly, no differences were found on growth performance, nutrient digestibility, and carcass characteristics of pigs during the finishing period among dietary treatments. As a result of the beta diversity analysis, microbial populations were not clustered between CON and GBR100 during the growing phase, but clustered into two distinct groups of CON and GBR100 during the finishing phase. In conclusion, brown rice can be added to the diets of growing-finishing pigs by replacing corn up to 100% without negatively affecting growth performance of the pigs; additionally, this may have an effect on changes in pig intestinal microbiota if continued for a long time.

172 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine from Corn Protein on Growth Performance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-62
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Control Diet ◽  
Average Daily Gain ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Negative Effects ◽  
Final Body Weight ◽  
Corn Protein ◽  
Dietary Treatments ◽  
Daily Gain

Abstract An experiment was conducted to test the hypothesis that addition of Val, Ile, or Trp alone or in combination will reduce the negative effects of excess Leu in diets for growing pigs. A total of 288 growing pigs (28.6 ± 2.5 kg) were randomly assigned to 1 of 9 dietary treatments in a randomized complete block design. There were 2 barrows and 2 gilts in each pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal and 8 diets based on corn and a high-protein corn product (48% crude protein) with 2 levels of crystalline L-Val (0 or 0.10%), L-Ile (0 or 0.10%), and L-Trp (0 or 0.05%) were formulated. The crystalline L-Val, L-Ile, and L-Trp increased standardized ileal digestible (SID) Val:Lys from 70 to 80%, SID Ile:Lys from 53 to 63%, and SID Trp:Lys from 18 to 23%, respectively. All diets were formulated to contain 1.00% SID Lys and the 8 diets containing corn protein contained 171% SID Leu:Lys. Individual pig weights were recorded at the beginning and at the conclusion of the 28-d experiment. Data were analyzed using the PROC MIXED of SAS with a pen as the experimental unit. Diet was the fixed effect and block and replicate within block were random effects. Results indicated that final body weight and average daily gain were not different between pigs fed the control diet and pigs fed the diet with Val and Trp addition, but greater (P &lt; 0.001) than for pigs fed the diet with Val addition, Ile addition, Trp addition, Val and Ile addition, Ile and Trp addition, or Val, Ile, and Trp addition (Table 1). In conclusion, addition of Val and Trp to diets with excess Leu may prevent negative effects of excess Leu in diets for growing pigs.

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