PSVII-18 Change in the taxonomic composition of ruminal microflora against the background of copper and plant extract

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 235-236
Author(s):  
Kseniya Atlanderova ◽  
Ayna Makaeva ◽  
Sergej Miroshnikov ◽  
Galimzhan Duskaev
Keyword(s):  
Plant Extract ◽  
Pathogenic Bacteria ◽  
Bos Taurus ◽  
Live Weight ◽  
Inhibitory Effect ◽  
Taxonomic Composition ◽  
Weight Group ◽  
Group Iii ◽  
Group I ◽  
Combined Use

Abstract Plant extracts are used in cattle diets to stimulate growth and immunity, while the unstable biochemical composition of plants contributes to the search for effective compositions that exhibit a synergistic effect. Objective of the study is the effect of an aqueous extract of oak bark (0.7 ml/kg of live weight) - group I, copper particles specific surface area 24 m2/g; 0.06 mg/kg of live weight - group II, their compositions (group III) on the taxonomic composition of the bacterial community of the rumen of cattle. Bulls had rumen fistulas Bos taurus at the age of 13 months (n = 5). Test samples of feed components were given with a concentrated portion of the main diet. Diet contain 60% - roughage (hay of Sudanese grass and alfalfa), 35% - concentrated feed (mixture of barley, wheat, sunflower husk, bran). The taxonomic composition of ruminal content was determined by NGS sequencing on a MiSeq device (Illumina, USA). It was found that the combined use of the extract and copper UFPs increases the concentration of representatives of Bacteroidetes in the rumen by 5.6%, the separate use of copper UFPs is by 20.7% (P ≤ 0.05), at the same time the values of representatives of Firmicutes decreased - by 7.2 and 24.7% (P ≤ 0.05) in comparison with the control. At the taxon level, the following classes prevailed: Bacilli (2.6%) in group I; Bacteroidia (17.0%) in II, in III - Bacteroidia (5.0%) in comparison with the control. In the experimental groups, at the genus level, a lower content of Streptococcus and the absence of Faecalibacterium were registered. Thus, the composition of plant extract and copper has an inhibitory effect on certain representatives of pathogenic bacteria. The results obtained indicate that the combined use of these substances is promising. This research was performed with financial support from the RSF (20-16-00088).

The body composition of 3-month-old Rambouillet lambs of different live weights

1968 ◽  
Vol 70 (1) ◽  
pp. 29-32 ◽  
Author(s):  
H. Epstein ◽  
A. Herz
Keyword(s):  
Live Weight ◽  
The Body ◽  
Testis Weight ◽  
Fat Tissue ◽  
Internal Organs ◽  
Weight Group ◽  
Group Iii ◽  
Group I ◽  
Dressing Percentage ◽  
Group Ii

SummeryCarcass measurements were made on 24 Rambouillet lambs of different live weights but of the same age, and the lambs divided into 3 groups of 8 according to live weight. Group I, with the lowest mean live weight, had the lowest dressing percentage, relatively heaviest head and feet and lightest pelt, while group III, with the highest mean live weight, had the highest dressing percentage, the relatively lightest head and feet and the heaviest pelt of the three groups.Group I contained the lowest percentage, and group II, of medium live weight, the highest percentage of muscle, while the relative weights of bone and fat tissue were similar. Group III contained the highest percentage of fat tissue and the lowest percentage of bone. The differences in the relative weights of the edible internal organs between the three groups were not significant.Testis weight was relatively much greater in Group III than in the lighter animals. While dressing-out percentage increased with body weight, the increase was not equally distributed between fore and hindquarters. Hindquarters were relatively larger in group II than in group I; but the forequarters accounted for the greater part of the differences between groups II and III.

scholarly journals Effect of feeding urea-molasses blocks with incorporated fenbendazole on grazing dairy heifers naturally infected with gastrointestinal nematodes

2003 ◽  
Vol 74 (2) ◽  
Author(s):  
R.M. Waruiru ◽  
C.O. Onyando ◽  
R.O. Machuka
Keyword(s):  
Weight Gain ◽  
Live Weight ◽  
Experimental Period ◽  
Gastrointestinal Nematodes ◽  
Group Iii ◽  
Dairy Heifers ◽  
Helminth Infections ◽  
Group I ◽  
Body Weights ◽  
The Mean

Between June 1999 and August 2000, the effects of feeding medicated urea-molasses supplement blocks on the growth of dairy heifers in a marginal area of central Kenya were assessed by comparing the live-weight gain of supplemented and unsupplemented heifers grazing the same pasture. Thirty-nine heifers with an average age of 9.6 months were initially treated orally with albendazole (10 mg / kg body weight) and assigned to 3 groups : group I was fed urea-molasses blocks with incorporated fenbendazole (MUMB), group II was fed urea-molasses blocks (UMB) and group III heifers (control) received no block supplementation (NBS). Body weights of the heifers and faecal egg counts (FECs) were measured monthly and larval cultures were made of positive faecal samples of each group. The mean cumulative live-weight responses of the MUMB and UMB groups were significantly greater than the NBS group (P < 0.05). However, at the end of the experimental period, the mean weight gain of the MUMB group did not differ from that of the UMB group (P >0.05). The FECs were moderate to low in all groups and decreased progressively with increasing age of the animals; FECs for the urea-molasses-supplemented groups remained significantly lower than those of the NBS group throughout the experimental period (P <0.05). Haemonchus and Trichostrongylus were the predominant nematode genera found in the heifers, but Cooperia, Bunostomum and Oesophagostomum were also present. These results indicate that feeding of urea-molasses blocks substantially reduced production losses attributable to nematode infection of young grazing cattle, and confirms previous observations that well-fed animals are better able to overcome the effects of helminth infections.

scholarly journals BEZERRAS DE CORTE INFECTADAS NATURALMENTE COM PARASITAS GASTRINTESTINAIS – EPIDEMIOLOGIA E TRATAMENTO SELETIVO

2005 ◽  
Vol 10 (1) ◽  
Author(s):  
M.B. MOLENTO ◽  
M. ALMEIDA ◽  
E. GUTERRES ◽  
J. ROMAN ◽  
F. FREITAS ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Body Condition Score ◽  
Lolium Multiflorum ◽  
Live Weight ◽  
Beef Heifers ◽  
Selective Treatment ◽  
Group Iii ◽  
Avena Strigosa ◽  
Group I ◽  
Parasite Challenge

A severidade das infecções parasitárias depende em grande parte da quantidade e do tipo de parasita presente e do estado nutricional dos animais. O objetivo deste experimento foi associar o efeito da suplementação protéica e pastagem de alta qualidade com diferenças na resposta ao desafio parasitário em 36 novilhas de oito meses, naturalmente infectadas, durante 120 dias. Grupo I: pastagem (Avena strigosa e Lolium multiflorum); Grupo II: pastagem e níveis crescentes de suplemento (Triticum aestivum, farelo de trigo) de 0,3 a 1,5% do peso vivo (PV); Grupo III: pastagem e suplemento a 0,9% do PV e Grupo IV: pastagem e níveis decrescentes de suplemento. O grau de infecção parasitária foi determinado através da contagem de ovos por grama de fezes (OPG) e coprocultura. Animais com OPG acima de 600 foram tratados com levamisole (Ripercol®, Fort Dodge). A coprocultura revelou os gêneros Cooperia spp. e Trichostrongylus spp. O ganho de peso, o escore corporal e o nível parasitário foi semelhante entre os grupos. Foram administradas 24 doses de anti-helmíntico com baixo grau de repetibilidade dos animais. Determinou-se que o tratamento seletivo pode ser aplicado quando os animais dispõem de pastagem de alta qualidade e com baixo desafio parasitário, independente de suplementação alimentar. Naturally infected beef heifers with gastrintestinal parasites – epidemiology and selective treatment Abstract The severity of parasite infections depends in part by the number and the parasite species as well as on the nutritional status of the host. The objective of this work was to determine the association between protein supplement and good quality pasture with parasite challenge infection in 36 naturally infected, 8 months old beef heifers during 120 days. Group I: pasture (Avena strigosa and Lolium multiflorum); Group II: pasture plus increasing levels of supplement (Triticum aestivum, wheat bran) from 0.3 to 1.5% of live weight (LW); Group III: pasture plus 0.9% LW of supplement; Group IV: pasture plus decreasing levels of supplement. Parasite infection was determined through fecal egg counts (EPG) and coproculture. All animals with EPG above 600 were treated with levamisole (Ripercol®, Fort Dodge). Coproculture displayed Cooperia sp. and Trichostrongylus sp. Weight gain, body condition score and parasite levels were similar among all groups. Twenty-four doses of the anthelmintic were administered with low animal repeatability. It was determined that the selective treatment may be used when animals have access to good quality pasture and a low parasite challenge, independently of the supplement offered.

scholarly journals PRODUCTIVE QUALITIES OF THE SIMMENTAL BREED BULLS BY PROBIOTICS VETOSPORIN SUSPENSION FEEDING

2018 ◽  
pp. 34-39
Author(s):  
Фаргат Вагапов ◽  
Fargat Vagapov ◽  
Наталья Гизатова ◽  
Natal'y Gizatova
Keyword(s):  
Live Weight ◽  
Group Iv ◽  
Feed Additive ◽  
Control Group ◽  
Dominant Position ◽  
Group Iii ◽  
Group I ◽  
Leading Position ◽  
Different Doses

The purpose of research is increase of beef productivity and beef quality of Simmental calves at introduc-tion in a diet of feeding different doses of the drug Vetosporin suspension. Of the half-yearly animals, 40 males were selected and formed into group IV for the experiment. The differences were only in feeding. The young were fed the studied additive Vetosporin suspension. In this case, the supplement was an addi-tion to the basic diet of animals of the experimental II-IV groups. The volume of the additive added was 0.1; 1.0; and 2 ml per 10 kg of live weight, respectively. Control group I, consumed exclusively a diet that does not contain an additive. Based on the results of the experiment at the age of 18 months. The prevalence of bulls of the experimental live weight over peers was observed, which was 4.4-25.3 kg (0.78-4.67%). The study of the studied indicators after control slaughter in the context of groups showed the superiority of bulls of experimental groups in all the periods studied. It is established that at the age of 15 months. The size of the removable live weight of the youngest of the control group was less by 8.0-19.7 kg (1.75-4.29%) than in the animals of the test groups. In this case, the leading position was occupied by the youngest of group III, the prevalence of which was 0.7-11.7 (0.14 -2.51%). It should be noted that after 3 months a simi-lar picture of the distribution of the studied quantities was observed. As for the output of carcass, we can note the following. Outsider among the animals of the experimental groups was the control group. So the bulls of group I were inferior to those who received the additive by 0.7-1.3%. At 18 months, as well as at 15 months, the dominant position was occupied by gobies of experimental groups. It should be noted that among the animals of the experimental groups the leading place was occupied by the bulls receiving the feed additive in a dose of 1.0 ml per 10 kg of live weight, that is, the youngest of the III group.

scholarly journals STUDIES ON TOXICOLOGICAL AND NEUROBEHAVIORAL PROFILE OF METHANOL EXTRACT OF MUSSAENDA ROXBURGHII HOOK. F. LEAVES IN MICE

2019 ◽  
pp. 295-301
Author(s):  
SANJIT NAMASUDRA ◽  
PANKAJ PHUKAN ◽  
MEENAKSHI BAWARI
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Morris Water Maze Test ◽  
Control Group ◽  
Group Iii ◽  
Total Distance ◽  
Maze Test ◽  
Group I ◽  
Group Ii ◽  
Distance Travel

Objective: The aim of this study was to evaluate the toxicity of Mussaenda roxburghii with special reference to the nervous system. Methods: For the study, 15 mice were obtained from Pasteur Institute, Shillong. The mice were then divided into three groups containing five mice in each group. The groups were, namely Group I, Group II, and Group III; Group I mice received distilled water and acted as a control group, Group II mice received plant extract at a dose of 600 mg/kg body weight (BWT.), and Group III mice received plant extract at a dose of 800 mg/kg BWT. The doses were administered orally through oral gavage tube for 28 days and the BWT of the mice was measured at 7th, 14th, 21th, and 28th days. The behavior of mice was evaluated for anxiety, locomotion, immobility, learning, and memory with the elevated plus maze test (EPMT), open field test (OFT), forced swim test (FST), and Morris water maze test (MWMT), respectively. Results: The result of the experiment showed a decrease in the BWT of mice exposed to plant extracts significantly as compared to the control. In the OFT, there is a significant decrease in total distance travel in OFT and also total distance travel in the central zone in mice treated with the plant extracts as compared to the control mice. In the EPMT, the plant extract treated mice showed a decreased in the time spent in open arms. The FST results in increased immobility in mice exposed to plant extracts as compared to control. In the present study, MWMT results in an increased escape latency and path length and in decreased annulus crossovers in plant extract treated group as compared to control. Conclusion: The results of the present study suggest that the plant extract alters the behavior of the treated mice and possesses neurotoxic activity.

scholarly journals PENGARUH PEMBERIAN Lactobacillus TERHADAP GAMBARAN MIKROSKOPIS MUKOSA USUS HALUS TIKUS WISTAR (Rattus norvegicus) YANG DIINFEKSI DENGAN Escherichia coli

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Steviany Towoliu
Keyword(s):  
Escherichia Coli ◽  
Wistar Rats ◽  
Pathogenic Bacteria ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
E Coli ◽  
Group 4 ◽  
Group I ◽  
Microscopic Features

Abstract: E. coli is part of the normal flora of the human and animal intestine and is commonly non pathogenic. However, one of the serotypes of this bacteria, which is enteropathogenic E. coli (EPEC), can cause primary infection on the intestine such diarrhea.  The growth of pathogenic bacteria in diarrheal patients can be inhibited by Lactobacillus.  Lactobacillus can function as probiotic which can affect the immune system of the digestive canals.  In addition, Lactobacillus also produce mucus  which can act as barrier to the pathogens.  The objective of this study was to reveal the effects of the administration of Lactobacillus on the microscopic features of the mucosa of the intestine of wistar rats infected by Escherichia coli.  This study was a laboratory experimental research employing 16 wistar rats divided into the control group (4 rats) and three treatment groups (12 rats) consisting of 4 rats each.  Results showed that; in group I the histological features were normal, in group II part of the epithelium of the mucosa showed erosion, dilatation of the capillary vessels, and many lymphosites were observed, in group III the epithelium of the mucosa was intact and the number of lymphosites was liitle, and in group IV the surface of the epithelium was intact, the presence of cell regeneration indicated by the increase number of goblet cells and a small number of  lymphocyes. Conclusions: The administration of after infection by E. coli has benefial effects indicated by the improvement of epithelial cells and the absence of denudation of the epithelium of the intestine. Keywords: E.coli, Lactobacillus, intestinal mucosa.   Abstrak: E.coli merupakan flora normal usus halus manusia dan hewan umumnya tidak menyebabkan penyakit.  Namun salah satu serotipe E.coli yaitu E.coli Enteropatogenik (EPEK) bersifat patogen dan dapat menyebabkan infeksi primer pada usus misalnya diare.  Pertumbuhan bakteri patogen pada pasien diare dapat dihambat oleh Lactobacillus. Lactobacillus merupakan probiotik yang akan mempengaruhi sistem imun saluran cerna serta memproduksi mukus yang berfungsi sebagai penghalang saluran cerna terhadap bakteri patogen.  Penelitian ini bertujuan untuk melihat efek pemberian Lactobacillus terhadap gambaran mikroskopis mukosa usus halus tikus wistar yang diinfeksi dengan Escherichia coli.  Penelitian ini merupakan penelitian eksperimental laboratorik.  Subjek penelitian terdiri dari 16 ekor tikur wistar yang dibagi dalam kelompok kontrol (4 ekor) dan kelompok  perlakuan (12 ekor) dibagi dalam 3 kelompok masing-masing 4 ekor.  Hasil penelitian kelompok I dengan gambaran histologik jaringan usus normal, kelompok II sebagian epitel mukosa usus halus terlihat erosi,  ada pelebaran pembuluh darah kapiler, dan banyak limfosit, kelompok III dengan permukaan epitel mukosa yang utuh dan jumlah limfosit sedikit, dan kelompok IV dengan permukaan epitel yang utuh, adanya regenerasi sel ditandai dengan bertambahnya sel goblet, dan sedikit limfosit.  Simpulan: Pemberian Lactobacillus setelah diberikan E.coli memberi efek yang baik berupa perbaikan sel epitel permukaan dan tidak terlihat denudasi epitel permukaan usus halus. Kata Kunci: E.coli, Lactobacillus, mukosa usus halus.

ВЗАИМОСВЯЗЬ ПРОДУКТИВНЫХ ПОКАЗАТЕЛЕЙ КОРОВ ЧЕРНО-ПЕСТРОЙ ПОРОДЫ С ВОСПРОИЗВОДИТЕЛЬНЫМИ КАЧЕСТВАМИ

2019 ◽  
pp. 39-42
Author(s):  
G.Yu. BEREZKINA ◽  
S.L. VOROBYEVA ◽  
E.M. KISLYAKOVA ◽  
A.A. KOREPANOVA
Keyword(s):  
Experimental Studies ◽  
Live Weight ◽  
Group Iv ◽  
Reproductive Capacity ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
The Republic ◽  
Breeding Farms

Изучено влияние возраста и живой массы при первом осеменении на воспроизводительные качества коровпервотелок чернопестрой породы в племенных заводах Удмуртской Республики. Исследования проводились в период с 2015 по 2018 гг. Для проведения исследований были сформированы 6 групп животных в зависимости от возраста первого осеменения. В I группу вошли коровы с возрастом первого осеменения до 13 мес, во II от 13,1 до 15 мес, в III от 15,1 до 17, в IV от 17,1 до 19, в V от 19,1 до 21 и в VI группу от 21 мес и старше. При этом живая масса телок в I группе в среднем составила 349,5 кг, во II 370,9, в III 387,7, в IV 400,2, в V 420,7 и в VI группе 440,2 кг. Анализ возраста первого осеменения в племенных хозяйствах республики показал, что средний возраст плодотворного осеменения в 2018 году составил 17,0 мес. с живой массой 394 кг. По результатам экспериментальных исследований нами получено, что лучшие воспроизводительные показатели и молочная продуктивность выявлена у коровпервотелок, которых осеменяли в возрасте 14,6 мес. В данной группе продолжительность сервиспериода составила 127,3 дня, коэффициент воспроизводительной способности 0,90, выход телят на 100 коров составил более 85,3. Удой за 305 дней лактации, у коровпервотелок второй опытной группы составил 6332,3 кг с массовой долей жира 3,65 и белка 3,15 .The influence of age and live weight at the first insemination on reproductive qualities of cowsborn black and white breed in breeding farms of the Udmurt Republic was studied. The studies were conducted between 2015 and 2018. For the research 6 groups of animals were formed depending on the age of the first insemination. Group I included cows with the age of first insemination up to 13 months, in group II from 13.1 to 15 months, in III group from 15.1 to 17 months, in group IV from 17.1 to 19 months, in group V from 19.1 to 21 months and in VI group from 21 months and more. At the same time, the live weight of heifers in group I averaged 349.5 kg, in group II370.9 kg, in group III 387.7 kg, in group IV 400.2 kg, in group V 420.7 kg and in group VI 440.2 kg. The analysis of the age of the first insemination in breeding farms of the Republic showed that the average age of fruitful insemination in 2018 was 17.0 months with a live weight of 394 kg. According to the results of experimental studies, we obtained that the best reproductive performance and milk productivity was revealed in cows, which were inseminated at the age of 14.6 months. In this group, the duration of the service period was 127.3 days, the coefficient of reproductive capacity 0.90, yield of calves per 100 cows. Milk yield for 305 days of lactation, in cows of the second experimental group was 6332.3 kg with a mass fraction of fat 3.65 and protein 3.15.

scholarly journals Molecular epidemiology of Shigella flexneri in a diarrhoea-endemic area of Lima, Peru

2004 ◽  
Vol 132 (2) ◽  
pp. 303-316 ◽  
Author(s):  
C. M. FERNANDEZ-PRADA ◽  
M. M. VENKATESAN ◽  
A. A. FRANCO ◽  
C. F. LANATA ◽  
R. B. SACK ◽  
...  
Keyword(s):  
Endemic Area ◽  
Pathogenic Bacteria ◽  
Shigella Flexneri ◽  
Plasmid Profile ◽  
Gene Probe ◽  
Community Based ◽  
Group Iii ◽  
Group I ◽  
Epidemiological Tool ◽  
Clonal Spread

A year-long community-based study of diarrhoeal diseases was conducted in Canto Grande, a periurban community in Lima, Peru. In 109 (34%) houses out of 323 that were visited, at least one individual was detected with shigellosis. The frequency of the 161 shigella isolates obtained was as follows: 117 S. flexneri (73%), 21 S. boydii (13%), 15 S. dysenteriae (9%), and 8 S. sonnei (5%). Using a non-radioactive ipaH gene probe as a molecular epidemiological tool, a total of 41 S. flexneri strains were shown to be distributed in 25 intra-family comparisons by pairs (icp). Further subdivision, based on a comparison of the serotype, plasmid profile, antibiotic resistances and ipaH hybridization patterns indicated that Group I, with 11 icp (44%), had strains that were identical, Group II with 8 icp (32%), had strains that were different and Group III with 6 icp (24%), had strains with the same serotype and identical ipaH profiles but with differences in other markers. This data indicates that a diversity of shigella clones circulated in this community resulting from both clonal spread and horizontal transfer of genetic elements. Furthermore, ipaH profiling of isolates can be used not only to differentiate between closely related shigella strains but also with other parameters, help to understand the dynamics of the generation of new clones of pathogenic bacteria.

scholarly journals Prediction of acute pancreatitis severity via the combined analysis of inflammatory biomarkers and coagulation parameters

2017 ◽  
Vol 25 (3) ◽  
pp. 237-244
Author(s):  
Snezana Tesic Rajkovic ◽  
Biljana Radovanovic Dinic ◽  
Miodrag Djordjevic ◽  
Goran Marjanovic ◽  
Sasa Grgov
Keyword(s):  
Acute Pancreatitis ◽  
Coagulation Factors ◽  
Inflammatory Biomarkers ◽  
Systemic Complications ◽  
Mathematical Formula ◽  
Coagulation Parameters ◽  
Group Iii ◽  
Group I ◽  
Combined Use ◽  
Group B

AbstractIntroduction. Timely assessment of severity of acute pancreatitis is needed to avoid severe systemic complications by making optimal therapeutic approach and correct prognosis of the disease. The aim of the study was to establish the role of several inflammatory biomarkers and coagulation parameters in prediction of AP severity, and also to propose a mathematical formula which allows their combined use for the same purpose. Material and Methods. The prospective study included 70 patients with AP. The patients were divided into groups: mild (group I), moderate (group II) and severe AP (group III). All patients were further classified into two groups: group A (mild AP) and group B (moderate and severe AP). Biochemical markers, inflammatory biomarkers and coagulation factors were tested in all patients. Results. Based on the results of Mann-Whitney,s test, it can be concluded that groups A and B are significant different from each other for CRP (p<0.05). Using the Wald’s stepwise forward method, a prediction model with CRP, PCT, D-dimer1, D-dimer3, fibrinogen1 and fibrinogen3 parameters as predictors of the severity of AP was obtained. The percentage of successful prediction of moderate or severe AP based on this model was 76.9%. The use of ROC analysis with the introduced linear combination from the logistic regression yielded equally good or even better results in the assessment of the severity of AP with the combined use of analyzed parameters. Conclusion. The combined analyses of biohumoral markers and coagulation parameters presented in the form a mathematical formula enabled a more accurate, rapid, rational and clinically available prediction of the severity of AP.

scholarly journals THE INFLUENCE OF THE INTENSITY OF BREEDING OF HEIFERS OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED IN DIFFERENT AGE PERIODS ON THE FUTURE DAIRY PRODUCTIVITY

2020 ◽  
Vol 59 ◽  
pp. 17-25
Author(s):  
S. F. Antonenko
Keyword(s):  
Live Weight ◽  
State Enterprise ◽  
Milk Productivity ◽  
Group Iii ◽  
Black And White ◽  
The Third ◽  
Group I ◽  
Animal Number ◽  
Group Ii ◽  
The Difference

The scientific experiment was carried out in two stages at State enterprise pilot farm “Hontarivka”, Vovchansk district, Kharkiv region. First stage. In a retrospective analysis of the rearing calf intensity from 3 to 6 months revealed that the highest live weight at 6-month age had animals of group III, .which is probably 47.0 kg or 34.6% and 23.0 kg or 14.4% (P < 0.001 in both comparison cases) outperformed peers I and II groups. A similar trend was observed in the analysis of live weight of heifers at 9 and 12-month age. Live weight at insemination in the higher growth group also had better rates of 4.0 and 3.7%, respectively, in animals of groups I and II (P < 0.001–0.05). It should be noted that the required live weight for insemination at heifers at growth rates of up to 500 g was reached in only 20.3 months, however, in the other two groups, the insemination age decreased by 1.5 and 1.7 months. Firstcalf heifers from group III also had the highest milk expectancy of 170.0 and 123.0 kg, or 3.1 and 2.3%, respectively, then animals in groups I and II. In the age period from 6 to 9 months it was found that at 9-month age heifers of group III had a higher live weight against animals of I and II groups respectively by 39.0 and 16.0 kg or 18.8 and 7.0% (P < 0.001 in both comparison cases). Intergroup differences by this indicator at 12-month age hiefers decreased to 35.0 and 8.0 kg, respectively, or 13.0 and 2.7% (P < 0.001 in both cases of comparison), which provided an advantage for the live weight of group III over I and II groups during the first fruitful insemination. Whereas the age of first fruitfull insemination, on the contrary, was lower at heifers of group III at 49 and 34 days than at groups I and II heifers. The highest milk productivity was also found at firstcalf heifers of group III at 634 kg or 12.3% (P < 0.001) and at 137 kg or 2.5% against animal I and II groups. The heifer live weight at the period from 9 to 12 months corresponded to the established differences at other age periods generally. And 12-month age calves of group III also significantly exceeded the analogues of group I by 38.0 kg or 14.0% (P < 0.001) and peers of group II – by 23.0 kg or 8.0% (P < 0.001). The live weight at fruitfull insemination of group III heifers was the largest and the difference compared with animals of group I was 21.0 kg or 5.3% (P < 0.01) and group II – 19.0 kg or 4.8% (P < 0.001). Group III animals also had the highest milk productivity, which is likely to be higher than group I peers by 751 kg or 14.5% (P < 0.001) and group II animals by 552 kg or 10.3% (P < 0.001). Second stage. It was found that during the experiment, live weight of free-stall keeping heifers were outnumbered by peers. In particular, the live weight of group I animals at the first month of the experiment was less by 8.0 kg or 2.9%, the second by 15.0 kg or 4.7% (P < 0.05) and the third by 19.0 kg or 5.4% against peers of group II. A similar pattern was observed in the estimation of intensive growth of these animals. However, at the end of the experiment, the heifers of the experimental group were 7.0 cm or 5.9% (P < 0.001) higher in the withers, 7.0 cm or 5.6% (P < 0.001) in the back, and the sacrum – 6.0 cm or 4.7% (P < 0.001). They showed deeper breasts by 3.0 cm or 4.9% (P < 0.01), larger breast widths – by 2.0 cm or 5.4% (P < 0.01), width in macaques – by 2.0 cm or 4.9% (P < 0.01), oblique torso length – 8.0 cm or 5.8% (P < 0.001), chest circumference 9.0 cm or 5.6% (P < 0.001) and girth – 1.0 cm or 6.1% (P < 0.01). It should be noted that from the second month of the research the animal number the free-stall keeping animal number, which ready for insemination, was more at 2.3 times or 5 goals than stall keeping heifers, and their live weight was more at 8 kg or 3.0%. With age, the differences between the groups gradually increased and by the third month reached 8 goals or 2.6 times and 19.0 kg or 5.7%.

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