PSXI-1 Influence of concentrations of essential and toxic elements in semen on qualitative characteristics of sperm of Holstein bulls

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 247-248
Author(s):  
Stanislav Platonov ◽  
Oleg Zavyalov ◽  
Alexey Frolov ◽  
Anatoly Kharlamov ◽  
Marina Kurilkina

Abstract Many studies show that an excess or deficiency of chemical elements in an animal’s body can lead to a number of reproductive disorders. In this regard, the aim of this study was to assess the influence of concentrations of essential and toxic elements in semen on qualitative characteristics of semen of sire bulls. The studies were carried out on Holstein bulls at the age of 3–4 years (n = 65). Sperm concentration (billions) was estimated using a digital photometer (IMV Technologies). Sperm activity (score) was studied using a phase contrast microscope (Nikon ECLIPSE E400, Tokyo, Japan). The elemental composition of the semen was determined by 25 chemical elements (Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, I, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Si, Sn, Hg, Sr, V, Zn) by ICP-AES and ICP-MS. Coefficients were calculated according to Spearman’s correlation. As a result of the research, it was found that sperm activity negatively correlated (r = -0.47; P ≤ 0.05) with the Pb content in semen and had a positive correlation with Zn concentrations (r = 0.55; P ≤ 0.05); Se (r = 0.64; P ≤ 0.05); P (r = 0.67; P ≤ 0.05). Sperm concentration was positively correlated with Cu levels (r = 0.55; P ≤ 0.05); P (r = 0.76; P ≤ 0.05); Se (r = 0.66; P ≤ 0.05). Thus, the results of the carried out studies indicate a high degree of relationship between the content of essential and toxic elements in semen and main qualitative characteristics of sperm. A deeper study of the role of chemical elements in reproductive function can be useful in overcoming the problem of low reproductive capacity of sires. The studies were carried out in accordance with the research plan for 2019–2021. FSBSI Federal Research Centre BST RAS (No. 0761-2019-0006)

2022 ◽  
Vol 42 ◽  
pp. 01019
Author(s):  
V. V. Kalashnikov ◽  
A. M. Zaitsev ◽  
M. M. Atroschenko ◽  
O. A. Zavyalov ◽  
A. N. Frolov ◽  
...  

Analysis of trace elements in blood serum can be an important tool for monitoring micronutrient supply in stallions in order to maintain reproductive health. In this regard, the purpose of this study was to determine the relationship between the concentrations of macro- and microelements in the blood serum of stud stallions with the qualitative characteristics of fresh sperm and sperm after cryopreservation and thawing. The studies were carried out on purebred Arabian stallions (n=50). As a biomaterial for studies, we used blood serum, fresh and thawed sperm after cryopreservation. The elemental composition of seminal plasma was determined by 25 chemical elements Co, Cr, Cu, Fe, I, Mn, Se, Zn Ca, K, Mg, P, B, Li, Si, V Na, Ni, Sn, Hg, Sr, Al, As, Cd, Pb) by ICP-DRC-MS methods. It was found that the concentrations of Mg and Co in the blood serum positively correlate with the qualitative characteristics of the sperm of Arabian purebred stallions. Wherein, the optimal range of concentrations of Mg in the blood serum, typical for stallions with relatively high indicators of sperm quality, is 22.64 to 27.05 μg/g; Co – 0.001 to 0.0015 μg/g.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 252-253
Author(s):  
Stanislav Platonov ◽  
Alexey Frolov ◽  
Anatoly Kharlamov ◽  
Oleg Zavyalov

Abstract It is known that chemical elements affect the quality characteristics of animal sperm. The purpose of this study was to assess the effect of the level of toxic load determined by the concentration of Al, Cd, Pb, Sn, Hg, Sr in wool on the quality characteristics of sperm. The studies were carried out on the Holstein bulls, the age at the selection of biosubstrates is 3–4 years (n = 65), which, based on data according to the amount of toxic elements (∑tox, mmol / kg) in hair, were divided into 3 groups: I - up to the 25th percentile, II - 25–75 percentile, III - above 75 percentile. Parameters evaluated were ejaculate in volume (ml) for 2 adjacent wells, sperm concentration (billions/ml) using a digital photometer, sperm activity (points) using a phase contrast microscope, the number of frozen doses (pcs), on average for the month preceding the selection of hair. Hair samples were taken from the withers in an amount of - 0.4 g. The elemental composition of wool was determined by 6 toxic elements (Al, Cd, Pb, Sn, Hg, Sr) by the AES-ICP and MS-ICP methods. According to the research results, it was established that as ∑tox increases from 0.114 mmol/kg in group I to 0.341 mmol/kg in group III, the volume of ejaculate decreases by 37.1% (Р£0,05), the concentration of sperm by 30.0% (Р£0,001), activity - by 0.2 points (Р£0,05), the number of frozen doses - by 182.4% (Р£0,001). Thus, the results of the studies carried out show the dependence of the quality indicators of sperm on the total content of toxic elements in hair of breeding bulls and it can be used as a diagnostic biosubstrate. The studies were carried out in accordance with the research plan for 2019–2021. FSBSI Federal Research Centre BST RAS (No. 0761-2019-0006).


2021 ◽  
Vol 0 (14) ◽  
pp. 40-48
Author(s):  
Elena Naryshkina

Abstract. Purpose of research. To estimate the impact of the season and age of the breeding bulls during the period of sperm collection on the main indicators of the own productivity of Holstein bulls. In studies of these indicators and productivity 229 bulls of Holstein breed have studied the effect of age (year of birth), season of the year, as well as the degree of influence of these factors on the value of indicators of productivity of bulls. Methods. Data on the main indicators of own productivity of breeding bulls are analyzed: the volume of ejaculate, the amount and concentration of spermatozoa in 1 ml. The research results were classified by season: winter, spring, summer, and autumn. The values of quantitative indicators of sperm production are given as deviations from the average value for the sample. Statistical analysis was performed using a boxplot diagram with average values and standard deviation (±σ) applied to it. The analysis of variance was performed using the following statistical model: yijk = µ + agej + seasonk + eijk + si. Fischer’s F-test was used to check the significance. Results. Regardless of the season, the values of the ejaculate volume and the total number of sperm in the ejaculate increase with age, and the concentration of sperm in 1 ml decreases. In the spring and summer period, 77–83 % and 66–79 % of observations had high values of the indicator volume of ejaculate and the number of spermatozoa in the ejaculate. In the spring and autumn periods, 62–64 % of observations had high values of sperm concentration in the ejaculate. The year of birth of the producer bull and the producer bull itself have a high degree of influence on the indicators of their own productivity: the volume of ejaculate – 14.94 and 18.22 %; the number of sperm in the ejaculate and their concentration – 8.53–27.88 and 4.51–15.82 %, respectively. Scientific novelty. The data obtained on the influence factors of season, age of the bull-the manufacturer’s figures for their own productivity.


2016 ◽  
Vol 40 (2) ◽  
pp. 152-155 ◽  
Author(s):  
TE Shishniashvili ◽  
NN Suladze ◽  
VV Margvelashvili

Objectives: To study the influence of environmental pollution on the mineralization of dental hard tissues by using biosubstrates: teeth and hair. :Study design: At the first stage epidemiological survey was conducted in polluted and less polluted areas of Tbilisi (Georgia). We studied 525 children aged 3 and 4 years. Caries prevalence and intensity was defined by the methodology of World Health Organization. At the second stage the chemical elements content was studied in hair and teeth hard tissues of 24 children by X - ray fluorescent spectroscopy method. Results: The prevalence of dental caries in the polluted region was 46%, caries intensity − 1.92 (± 2.842). In the less polluted region prevalence was 37%, caries intensity − 1.47 (± 2.571). These data are statistically reliable (p < 0.05). The study of hair and tooth tissues showed that the toxic elements (Pb, Hg, Sn, Ti) content in these tissues was higher in environmentally unfavorable than in favorable conditions. Conclusion: Hair and dental tissues can be used as indicators of environmental pollution. Our survey showed that toxic elements content in dental hard tissues was higher and the level of essential elements was less in polluted than in less polluted areas of Tbilisi.


2021 ◽  
pp. 112331
Author(s):  
Francesca Ferraris ◽  
Francesca Iacoponi ◽  
Andrea Raggi ◽  
Francesca Baldi ◽  
Murielle Fretigny ◽  
...  

2001 ◽  
Vol 3 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Jon Øyvind Odland ◽  
Evert Nieboer ◽  
Natalya Romanova ◽  
Yngvar Thomassen ◽  
Dag Hofoss ◽  
...  

Author(s):  
Mohamed Anouar Nouioui ◽  
Manel Araoud ◽  
Marie-Laure Milliand ◽  
Frédérique Bessueille-Barbier ◽  
Dorra Amira ◽  
...  

2018 ◽  
Vol 56 ◽  
pp. 136-140
Author(s):  
G. S. Sharapa ◽  
O. V. Boyko

Scientific and production experiments were carried out on cows of Ukrainian Black-and-White diary breed (UBAWD) of SE "Seagull" (branch "Lisne") during 2016–2017. The anatomical and physiological state of genital organs of cows was determined by the method of clinical and gynecological research of animals before conducting experiments and during them every 40–50 days. In total 486 cows were kept under control, which were kept in typical rooms with running platforms, but the active motivation of animals was clearly insufficient. Feeding cows according to the recommended standards. The milk yield from the cow in 2017 was 7813 kg. The experiments studied the effect of feeding cows during the dry period, the conditions of retention and some other paratypic factors on the course of the genera and the postnatal period, the duration of the service period and the fertility of cows. In analyzing the research results, personal records and veterinary documentation were used. In conducting experiments, it was found that normal calving was in 89% of cows, and severe and pathological – in 11% of cases. The main diseases in the afterlife period were: detention of the litter – 9.5%, postpartum endometritis – 21.5%, hypofunction of the ovaries – 23.0%, persistent yellow ovarian bodies – 25.6% and others. Normal intervals between stages of excitation of the sexual cycle (18–22 days) were in 63% of cows, and violations of the rhythm of sexual cycle, associated with dysfunction of the ovaries and missed the hunt – 30% of animals. Subunvulation of the genital organs of the cows contributed to the diseases of the limbs, udder and digestive disorders. During 2016–2017, the effect of feeding cows (173 g.) in the dry period on the course of calving, post-autumn period and their fertility was studied. Under the same conditions, keeping cows in the maternity ward and feeding rough and succulent feeds concentrated feeds fed cows of the control group (98 heads) to 1–2 kg daily during the dry period, and experimental (75 heads) – for 18–20 days to calving 2–3 kg. A positive difference was found in favor of cows of the experimental group: there was a more active stage of expulsion of the fetus, less delay of the placenta (by 7%) and endometritis (by 12%), the duration of the postoperative regenerative period decreased by 8.6 days; service period – 19.0 days; fertility from two inseminans was higher by 5.7%. Study of the reproductive capacity of cows at the hotel in different seasons showed that the recovery period was shorter by 11 days in the presence of cows in 3–4 quarters, and the service period – by 23.2 days at hotel in I and IV sq. The average duration of the recovery period was 79.5 days, while the service period was 123.4 days. The longest duration of the joint ventricle was in cows at hotel during the hot summer days of the year. The average fertilization rate from the first insemination of 339 cows was 44.9% with fluctuations in individual groups of milkers from 26.8% to 55.0%. The following indicators were in the number 1 and number 3 (respectively 26.8% and 31.4%). The best fertility rates were in groups № 4, 6 and 7, where there were more cows of the second and third lactation, and the responsible attitude of the milkers to work in organizing cows walks and sexually explicit hunting. When comparing the assessment of the duration of the IP and SP in full-age cows and cows, the first-born, taking into account 225 goals. It was found that these indicators were longer in primitive cows – 20.7 days respectively and 21.9 days in relation to full-age cows. The obtained results are completely consistent with the results obtained by a number of both domestic and foreign researchers. More attention is needed to grow heifers and to prepare newborns for delivery. In 15–17% of cows at and after the hotel, gynecological diseases that occurred in the presence of gastrointestinal diseases, lameness, mastitis and others were diagnosed. This has negatively affected the overall performance of the herd. In studying the duration of HP and SP in healthy and sick cows after calving, it was found that if the SP in healthy cows lasts 73.4 days, then in patients – 116.3 days (the difference was 42.9 days), and the difference in the duration of the joint venture reached 98.7 days. On the basis of the results of the research, it can be concluded that the reproductive function of the cows is influenced by such paratypic factors as feeding animals in the dry period, the temperature factor (time of year), the human factor (the effect of milking on a group of cows), age and physiological state of cows in the hotel and in the afterlife period. When feeding 2–3 kg of bovine feed for cows, together with rough and juicy fodder for 18–20 days prior to calving, the recovery period decreased by 8.6 days and service period – by 19 days, and fertility from two inseminals increased by 5.7%, reaching 85.3%. The smallest duration of the service-period was in the presence of cows in the I and IV quarters year (107.7–117.1 days). In primary cows, the recovery period was longer than that of full-age cows by 20.7 days, and the service period was 21.9 days. In the physiologically healthy cows after calving, the recovery period lasted an average of 73.4 days and service period – 98.7 days, and in patients, after the prescribed treatment – respectively 116.3 days and 197.4 days.


2021 ◽  
Vol 11 (2) ◽  
pp. 157-169
Author(s):  
Pavlo Skliarov ◽  
Serhiy Fedorenko ◽  
Svitlana Naumenko ◽  
Oleksandr Onyshchenko ◽  
Alina Pasternak ◽  
...  

Animal reproduction is one of the main factors limiting the efficiency of livestock production. Its optimal level is possibly achieved when certain conditions are created for animals. As reproduction is a complex reflex process depending on neuroendocrine regulatory mechanisms, the character and strength of stimuli, which, in turn, is due to a number of factors. Under normal conditions, the body of animals is affected by many different factors, which are appropriately transformed and specified by positive or negative reactions. Inhibitory factors include air pool, saturated with harmful substances and gases, ionizing radiation, poor water quality along with altered redox properties, hypokinesia combined with poor unbalanced feeding, systematic chronic stress, presence of toxic substances in feed, and the deficiency of vitamins and other bioantioxidants in feed or their excessive spending. Of the wide range of genetic and paratypic factors of negative impacts on reproductive capacity, the most common one is alimentary, which causes impaired reproductive function due to deficiencies in the rules, regulations, and feeding regime of animals. In particular, the alimentary can be associated with both general malnutrition (starvation) and overfeeding (obesity). However, the alimentary form of infertility mostly occurs due to low-quality diets when the diet lacks vital components (mainly vitamins, macro-, and micronutrients) or the quantitative ratios of the ingredients are violated. This is possible even if the total nutritional value of the diet meets the established requirements for the physiological needs of the body. Vitamins, micro-, and macronutrients are ecologically deficient factors of disturbance of animal reproductive function, the influence of which is observed on all processes of reproduction, from fertilization to the postpartum period and the preservation of young animals. The pathogenesis of their insufficiency is associated with the violation of steroido-, gameto-, and embryogenesis and the emergence of ante-, intra-, neo- and postnatal pathologies, respectively. Therefore, treatments and prevention measures should be aimed at providing animals with biologically complete balanced feeding and replenishment of the body with vitamins and minerals. However, all these issues remain incompletely studied and need further research.


Sign in / Sign up

Export Citation Format

Share Document