PSIV-10 Effects of chronic molybdenum exposure in drinking water or feed on molybdenum and copper status and production performance of gestating and lactating beef cattle consuming a low-quality forage diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 294-295
Author(s):  
Meghan P Thorndyke ◽  
Octavio Guimaraes ◽  
Matheus Medrado ◽  
Briana V Tangredi ◽  
Huey Yi Loh ◽  
...  

Abstract The objective of this experiment was to evaluate the effects of Mo source (feed or water) on performance and mineral status of cows and calves fed a forage-based diet for two years (DM basis: 6.6% CP; 0.14% S, 6.7 mg Cu/kg, 2.4 mg Mo/kg). Fifty-four cow-calf pairs were stratified by cow age, BW, and liver Cu and Mo status, and randomly assigned to one of six treatments. Treatments consisted of: 1) Negative control (NC; no supplemental Mo or Cu); 2) Positive control [PC: NC + Cu (3 mg Cu/kg diet DM from CuSO4·5H2O)]; 3) NC + 500 µg Mo/L from MoNa2O4·2H2O in drinking water (Mo 500-water); 4) NC + 1000 µg Mo/L of MoNa2O4·2H2O in drinking water (Mo 1000-water); 5) Mo 1000-water + 3 mg Cu/kg diet DM from CuSO4·5H2O (Mo 1000-water+Cu); and 6) NC + 3 mg Mo/kg diet DM from MoNa2O4·2H2O (3.0 Mo-diet). Cattle were allowed ad-libitum access to feed, water, and a protein supplement throughout the experiment. During the winter months animals were housed in three replicate pens per treatment and during the summer months animals were housed in separate pastures by treatment where cow and calf feed and water intake could be measured separately. Data were analyzed as a completely randomized block design. Throughout the experiment no signs of molybdenosis were observed. There was a treatment x time (P < 0.01) interaction for cow liver Cu concentrations. Cows not receiving supplemental Cu had lower (P < 0.01) liver Cu concentrations than cows receiving supplemental Cu. Cow and calf performance, calf mineral status, cow liver Mo, and plasma and milk Mo and Cu concentrations were similar across treatments. These data indicate that Mo supplemented in water or feed at concentrations used in this experiment had minimal impact on Cu status and overall animal performance.

2016 ◽  
Vol 37 (4Supl1) ◽  
pp. 2677 ◽  
Author(s):  
Paula Rodrigues Oliveira ◽  
Fabiana Ramos Santos ◽  
Eduardo Ferreira Duarte ◽  
Gustavo Silva Guimarães ◽  
Nadiessa Sartori Carvalho Mattos ◽  
...  

This study aimed to test the effects of dietary Aloe vera and Symphytum officinale extracts added separately or in combination with symbiotics on the performance, nutrient utilization, serum biochemical parameters, biometrics, and intestinal histomorfometry of broilers. The experiment had a randomized block design with five treatments and six replicates of ten broilers each. Treatments were as follows: negative control and positive control (diet without and with antibiotic, respectively); 0.2% Aloe vera (AV); 0.2% Symphytum officinale (S); 0.2% functional supplement, composed of symbiotics fermented in Aloe vera and comfrey plant extracts (S+PE). At seven days of age, FI of birds fed the Aloe vera extracts diets were lower than that observed for birds consuming the diet with Symphytum officinale extract and S+PE. Broiler performance remained unaffected by treatments at others ages evaluated. At 10 to 14 days of age the lowest ADCDM ADCCP was shown in group feed NC. The highest ADCCP was observed in PC control group and in diets supplemented with Aloe vera and S+PE. Serum levels of cholesterol, triglycerides, and phosphorus were affected by addition of extracts at seven, 21, and 35 days of age. The longest duodenal villi were observed in broilers fed S+PE diets at seven days of age. Aloe vera and Symphytum officinale extracts and symbiotics can be used in broiler diets as an alternative to growth-promoting antibiotics.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 398-399
Author(s):  
Sinthia Siqueira ◽  
Hébert De Brito ◽  
Wagner Araújo ◽  
Hélio Neves ◽  
Mara Benfato ◽  
...  

Abstract The present study aimed to evaluate the impact of a mycotoxin deactivator in diets containing added mycotoxins for gestating and lactating gilts on their performance. A total of 60 gilts (LD x LW) were used. After insemination the gilts were distributed among 3 dietary treatments according to a randomized block design: a negative control (CON); mycotoxin positive control (Myc); and a positive control added a mycotoxin deactivator (Myc+Deact 2 kg/ton; Unike®Plus, Adisseo). The total mycotoxins levels added to the diets 2 and 3 from d 75 of gestation until farrowing were: DON at 2.5 mg/kg, FBs at 10 mg/kg, and ZEA at 0.75mg/kg. Sows received the experimental diets from d 75 of gestation until weaning (i.e. 21 d). At farrowing, total number of piglets born, born alive, stillborn, and mummies were measured. Piglets were individually weighed 24-h post-farrowing, and at weaning to determine litter performance during lactation. The effects of diet composition, blocks and initial weight were tested according to a general linear procedure analysis of variance (GLM procedure of SAS). During gestation, treatments influenced (P = 0.022) sow mortality rate: CON had no mortality and Myc 15.8% and Myc+deact 10.5%. Same observation was made for the lactation period (P = 0.017): CON showed a lower mortality rate compared to the mycotoxin fed sows (5.0 vs. 31.2 vs. 11.8%, respectively for CON, Myc and Myc+deact). Sow milk production yield was greater (P = 0.021) in CON (11.66 vs. 9.53 vs. 9.67 kg/ d, respectively for CON, Myc and Myc+deact). As a consequence of that, treatments also influenced piglet (P = 0.047) and litter (P = 0.048) weight with a higher value for CON sows, followed by Myc+deact and Myc. Our findings showed that the use of the deactivator can mitigate the negative effects on performance when animals are subjected to diets contaminated by challenging levels of mycotoxins.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 436-437
Author(s):  
Meghan P Thorndyke ◽  
Octavio Guimaraes ◽  
Nicole M Tillquist ◽  
Briana V Tangredi ◽  
Joanis T Zervoudakis ◽  
...  

Abstract Fifty-four multiparous beef cows were used to examine the effect of molybdenum (Mo) supplemented in drinking water or feed on offspring performance. Cows were blocked by body weight (BW) and age into one of 6 groups. Group were then randomly assigned to treatment. Treatments consisted of: 1) Control (no supplemental Mo or Cu), 2) Control+Cu (3 mg Cu/kg DM from CuSO4·5H2O added to the basal diet), 3) Control + 500 µg Mo/L from MoNa2O4·2H2O in drinking water (Mo 500-water), 4) Control + 1000 µg Mo/L of MoNa2O4·2H2O in drinking water (Mo 1000-water), 5) Mo 1000-water plus 3 mg Cu/kg DM from CuSO4·5H2O added to the basal diet (Mo 1000-water+Cu, and 6) Control plus 3 mg Mo/kg DM from MoNa2O4·2H2O added to the basal diet (3.0 Mo-diet). Cows were housed in dry lot pens (n = 3 cows/pen; 3 pens/treatment) and fed a low-quality grass hay diet (DM basis: 6.6% CP; 0.14% S, 6.2 mg Cu/kg, 2.3 mg Mo/kg) and a protein supplement (30% CP). Cows received their respective treatments beginning 60 d prior to breeding and remained on treatments until all calves were weaned at approximately 7 mo. of age. Calf birth weights were collected on the day of birth and all calves were weaned on the same day. Data were analyzed using a mixed effects model for a completely randomized block design. Birthweight, ADG, and 205d adjusted weaning weights were similar (P > 0.05) across treatments. These data indicate that Mo supplemented in the drinking water or the diet regardless of the inclusion of additional Cu did not impact calf performance through weaning.


2020 ◽  
Vol 25 (1) ◽  
pp. 22
Author(s):  
Rusliandi Rusliandi ◽  
Diah Wulandari Rousdy ◽  
Mukarlina Mukarlina

Humic acid is a humus compound found in peat soil. Humic acid can potentially be used as an anti-inflammatory compound. This study aimed to determine the effect of humic acid on the volume of foot mice edema and to find the best dose that can suppress the degree of edema volume. The animal object was Swiss mice weighing 25-30 grams and 3 months old. The study used Randomized Block Design (RBD) with positive control, negative control and humic acid treatment with dose 62.5 mg kg-1BW, 125 mg kg-1BW, and 250 mg kg-1BW. The result of this research showed that edema inhibition by the administration of humic acid dose 62,5; 125; 250 mg kg-1 had inflammatory inhibition percentage 2.67%, 13.34%, and 20.01% respectively in 5-hour observation. The best dose of humic acid to suppress inflammation in the mice's paw is a humic acid dose of 250 mg kg-1 compared with value 23.3% of sodium diclofenac as the positive control.


2020 ◽  
Vol 14 (01) ◽  
pp. 23
Author(s):  
Inten Sharon Datuyanan ◽  
Bistok Hasiholan Simanjuntak ◽  
Andree Wijaya Setiawan ◽  
Yoga Aji Handoko

As food contains vitamin, mineral and antioxidant, beetroot (Beta vulgaris L.) had beneficial for health. Although beetroot contains a lot of nutritional value, beetroot is less liked by the public because of an earthy taste. For this reason, it needs innovation of the beetroot processing into products that can be accepted by the public, such as beverage fruit drink with food additives. In this study, beetroot juice with food additives, i.e. lemongrass, and turmeric mango was expected to improve taste and acceptable consumer. This study were aimed to analyze the effect of lemongrass and turmeric mango on physicochemical characteristics and determine the organoleptic quality of beetroot juice. This study used a Randomized Block Design (RBD) with the addition of lemongrass and turmeric mango respectively 1%, 3%, 5% concentration in the beetroot juice. Negative control used beetroot juice plain and the positive control used beet juice plus honey. Data were analyzed by variance (ANOVA), if the results obtained were significantly different, the DMRT continuing with 5% significant level. The results showed that the addition of lemongrass and turmeric mango with the concentration of each 1%, 3%, 5% effect on the physicochemical characteristics and organoleptic beet juice. The highest total dissolved solids was 6.92oBrix and the highest betacyanin content of 10.26 mg/L occurred at the addition of 5% turmeric mango. The highest pH value was shown in the negative control (beetroot juice plain) with an average of 6.52. The highest vitamin C content found in the addition of 1% lemongrass about 6.16 mg/100g. The results also showed that the organoleptic beet juice with the addition of 1% lemongrass and the positive control (beet juice plus honey) as a sample that it the most preferred by the panelists. Keywords: beetroot juice, lemongrass, physical-chemistry, organoleptic, turmeric mango


EUGENIA ◽  
2011 ◽  
Vol 17 (2) ◽  
Author(s):  
Aenul Latifah ◽  
. Kustantinah ◽  
Loekas Soesanto

ABSTRACT The green house research aimed to know the best isolate of T. harzianum in controlling Fusarium wilt , shallot production, and the effect of T. harzianum application time in suppressing the disease. Randomized block design was used with twelve treatments. Each treatment was replicated three times. The treatments were negative control, positive control as wel as extract of ginger isolate, banana isolate, shallot isolate, potato isolate, and ginseng isolates. The treatments were  applied a week before or after inoculation of the pathogen. The result showed that the antagonist extract of ginger isolate applied a week before inoculation decreased the disease intensity by 43.854%. The antagonist extract application applied before or after inoculation were not significant difference . The antagonist of potato isolate extract was the best in increasing number of plants per clump and tuber fresh weight resulting  9.7503 and 13.133 g, respectively. The best extract in increasing root growth was shallot extract. The ginger isolate extract increased root length by 17.672%. Keywords: Trichoderma harzianum isolates, biological control, Fusarium wilt, shallot   ABSTRAK Penelitian rumah kasa ini bertujuan untuk mengetahui isolat T. harzianum yang paling baik dalam mengendalikan penyakit moler dan meningkatkan hasil bawang merah, serta pengaruh waktu pemberian T. harzianum dalam menekan penyakit moler. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK), yang terdiri atas 12 perlakuan dengan 3 ulangan. Perlakuan yang dicoba adalah kontrol negatif (tanpa perlakuan T. harzianum dan  Fusarium oxysporum) dan kontrol positif (hanya dengan inokulasi F. oxysporum), serta ekstrak isolat jahe, pisang, bawang merah, kentang, dan ginseng yang diberikan 1 minggu sebelum atau sesudah inokulasi. Hasil penelitian menunjukkan bahwa ekstrak T. harzianum isolat jahe yang disiram sebelum inokulasi Fusarium oxysporum, mampu menurunkan intensitas penyakit sebesar 43,854%. Belum terlihat jelas pengaruh nyata pemberian ekstrak antagonis sebelum maupun sesudah inokulasi patogen. Ekstrak T. harzianum isolat kentang adalah isolat terbaik dalam meningkatkan jumlah anakan per rumpun dan bobot basah umbi, yaitu masing-masing 9,7503 dan 13,133 g, sedangkan ekstrak terbaik dalam pertumbuhan akar yaitu isolat bawang merah yang meningkatkan jumlah akar sebesar 57,452% dan ekstrak isolat jahe menunjukkan pengaruh terbaik meningkatkan panjang akar sebesar 17,672%. Eugenia Volume 17  No. 2  Agustus 2011 Kata Kunci: Isolat Trichoderma harzianum, pengendalian hayati, penyakit layu Fusarium, bawang merah


Plant Disease ◽  
2016 ◽  
Vol 100 (10) ◽  
pp. 2094-2098 ◽  
Author(s):  
Saskia G. von Diest ◽  
Julia C. Meitz-Hopkins ◽  
William E. MacHardy ◽  
Cheryl L. Lennox

The South African apple industry currently relies entirely on chemical fungicides to control apple scab (Venturia inaequalis). In this study, the effectiveness of sanitation strategies in reducing scab incidence and severity in South African orchards was evaluated. Over three seasons, leaf shredding with no fungicide sprays was tested against a nonsprayed, nonshredded negative control, a positive control that followed a commercial fungicide program, and a combined treatment of a commercial fungicide program with leaf shredding. Two treatment replicates were applied in a randomized block design in each of two orchards. Scab incidence and severity on fruit and leaves were assessed weekly from green-tip until fruit-set in the following spring. Pooled data from the 3 years revealed that fruit scab incidence and severity and leaf scab severity (51, 55, and 39%, respectively, P < 0.05) and leaf scab incidence (33%, P < 0.1) were significantly lower in the leaf-shredding treatment than in the negative control. This is the first study to evaluate the effect of leaf shredding in reducing scab in South African orchards. Results indicate that this treatment is highly effective and should be integrated into scab management strategies in future, but should be customized to suit South African orchard conditions.


2020 ◽  
Vol 45 (4) ◽  
pp. 305-319
Author(s):  
L. Istiqomah ◽  
A. A. Sakti ◽  
A. Sofyan ◽  
H. Herdian ◽  
A. S. Anggraeni

This study aimed to evaluate the administration of Lactobacillus plantarum AKK-30 and Saccharomyces cerevisiae B-18 as probiotics in reducing cholesterol of blood, egg, and meat and also the production performance of laying quail (Coturnix coturnix Japonica). A number of 600 birds of the twenty one-day-old quails were distributed in a completely randomized design with 5 treatments and 6 replications (initial BW= 101.35 ± 1.64 g). Treatments were as follows: A = negative control (basal diet without probiotic), B = 1% of L. plantarum AKK-30 (107 cfu/g), C = 1% of S. cerevisiae B-18 (106 cfu/g), D = 1% of a probiotic consortium, E = positive control (commercial probiotic). Parameters observed were blood biochemistry profiles, product quality, and production performance. The results revealed that administration of 1% S. cerevisiae (C) resulted the lowest cholesterol in blood (101.75 mg/dL) and egg (9.44 mg/g) and while administration of 1% L. plantarum(B) increased meat protein and decreased fat content (P<0.05). Level of blood triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were not different among treatments. Probiotic treatments did not affect the growth performance, quail day production (QDP), feed conversion ratio (FCR) and egg uniformity. However, probiotic increased diameter of egg yolk (P<0.05). It was concluded that administration of single probiotic L. plantarum AKK-30 or S. cerevisiae B-18 improves the quality of laying quail products. 


2020 ◽  
Vol 8 (2) ◽  
pp. 1
Author(s):  
Renato Anastácio Guazina ◽  
Rosiane Araujo Rodrigues Nass ◽  
Gustavo De Faria Theodoro ◽  
Ricardo Carneiro Brumatti ◽  
Sofia Michele Muchalak ◽  
...  

This study was carried out to determine whether the yield of soybean cultivars and the severity of Asian Soybean Rust (ASR) are influenced by foliar spray of silicon exclusively or along with a fungicide mixture, as well as the economic viability of their use for a sustainable crop. The experiment was performed in randomized-block design, five replicates, two soybean cultivars and (2×6) + 2 factorial arrangements: S1 (AlSi at R1 + R4); S2 (AlSi at R1 + R5.1); S3 (AlSi at R1 + FM at R4); S4 (FM at R1 + AlSi at R4); S5 (AlSi at R1 + FM at R5.1); S6 (FM at R1 + AlSi at R5.1); NC and PC (negative and positive control). The economic evaluation and sensitivity analysis were undertaken for Anta 82 RR. There was no interaction effect between the factors, and cultivar CD 2737 had a higher yield. ASR was less severe in CD 2737, and S3 and S5 provided the lowest disease severity. Exclusive spray of aluminum silicate was able to reduce the number of lesions with uredinia, open uredinia and uredinia per lesion. S3 increased grain yield, resulting in higher revenues and a 66.1% gross margin. Sensitivity analysis revealed that S3 was profitable in all scenarios.


2019 ◽  
Vol 49 ◽  
Author(s):  
Bruno Zuntini ◽  
Rita de Cassia Félix Alvarez ◽  
Gustavo de Faria Theodoro ◽  
Alan Mario Zuffo

ABSTRACT Fungicides used to control the Asian soybean rust are composed almost entirely of mixtures involving the systemic (triazoles) and meso-systemic (strobilurins) modes of action. However, to control the soybean downy mildew, only protective fungicides are available. The present study aimed to evaluate the effectiveness of fungicides with different modes of action in the control of downy mildew and Asian soybean rust. A field experiment was carried out in a randomized block design, with seven treatments [negative control; difenoconazole at V7 + (azoxystrobin + cyproconazole) at R2 and R5.1; (tebuconazole + carbendazim) at V7 + (azoxystrobin + tebuconazole) at R2 and R5.1; carbendazim and mancozeb at V7 + (azoxystrobin + tebuconazole) at R2 and R5.1; (tebuconazole + carbendazim) at V7 + mancozeb in V7 + (azoxystrobin + tebuconazole) at R2 and R5.1; (tebuconazole + carbendazim) at V7 + (azoxystrobin + tebuconazole) at R2 and R5.1 + mancozeb at R2 and R5.1; (tebuconazole + carbendazim) at V7 + azoxystrobin + tebuconazole at R2 and R5.1 + mancozeb at R2 and R5.1 + carbendazim at R5.1] and four replicates. The following variables were evaluated: disease severity based on diagrammatic scales, phytotoxicity, product effectiveness, defoliation, number of pods per plant, number of seeds per pod, 1,000-seed weight and yield. The treatments with mancozeb addition showed to be more efficient in reducing the severity of mildew and Asian soybean rust and defoliation, providing increased seed yields for the crop. For the soybean downy mildew, the treatments with addition of mancozeb to benzimidazole, strobilurins and triazoles were more efficient in reducing the severity and for the control.


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