scholarly journals PSXII-12 The influence of long-term molybdenum supplementation (in drinking water or feed) on beef calf performance through weaning

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 436-437
Author(s):  
Meghan P Thorndyke ◽  
Octavio Guimaraes ◽  
Nicole M Tillquist ◽  
Briana V Tangredi ◽  
Joanis T Zervoudakis ◽  
...  
Keyword(s):  
Drinking Water ◽  
Block Design ◽  
Basal Diet ◽  
Beef Cows ◽  
Mixed Effects ◽  
Protein Supplement ◽  
Offspring Performance ◽  
Randomized Block Design ◽  
Calf Performance

Abstract Fifty-four multiparous beef cows were used to examine the effect of molybdenum (Mo) supplemented in drinking water or feed on offspring performance. Cows were blocked by body weight (BW) and age into one of 6 groups. Group were then randomly assigned to treatment. Treatments consisted of: 1) Control (no supplemental Mo or Cu), 2) Control+Cu (3 mg Cu/kg DM from CuSO4·5H2O added to the basal diet), 3) Control + 500 µg Mo/L from MoNa2O4·2H2O in drinking water (Mo 500-water), 4) Control + 1000 µg Mo/L of MoNa2O4·2H2O in drinking water (Mo 1000-water), 5) Mo 1000-water plus 3 mg Cu/kg DM from CuSO4·5H2O added to the basal diet (Mo 1000-water+Cu, and 6) Control plus 3 mg Mo/kg DM from MoNa2O4·2H2O added to the basal diet (3.0 Mo-diet). Cows were housed in dry lot pens (n = 3 cows/pen; 3 pens/treatment) and fed a low-quality grass hay diet (DM basis: 6.6% CP; 0.14% S, 6.2 mg Cu/kg, 2.3 mg Mo/kg) and a protein supplement (30% CP). Cows received their respective treatments beginning 60 d prior to breeding and remained on treatments until all calves were weaned at approximately 7 mo. of age. Calf birth weights were collected on the day of birth and all calves were weaned on the same day. Data were analyzed using a mixed effects model for a completely randomized block design. Birthweight, ADG, and 205d adjusted weaning weights were similar (P > 0.05) across treatments. These data indicate that Mo supplemented in the drinking water or the diet regardless of the inclusion of additional Cu did not impact calf performance through weaning.

scholarly journals PSXII-18 The influence of molybdenum in drinking water or feed on apparent absorption and retention of molybdenum and copper in beef steers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 423-424
Author(s):  
Meghan P Thorndyke ◽  
Nicole M Tillquist ◽  
Nicole M Jones ◽  
Terry Engle
Keyword(s):  
Drinking Water ◽  
Dry Matter ◽  
Block Design ◽  
Sodium Molybdate ◽  
Mixed Effects ◽  
Dry Matter Intake ◽  
Beef Steers ◽  
Apparent Absorption ◽  
Randomized Block Design ◽  
Intake Control

Abstract Twelve Angus steers (BW 694.1 ± 47.4 kg) fitted ruminal cannulae were used to examine the effect of molybdenum (Mo) supplemented in drinking water or feed on apparent absorption and retention of Mo and copper (Cu). Steers were fed a low-quality grass hay diet (DM basis: 6.5% CP; 0.13% S, 3.4 mg Cu/kg, 2.4 mg Mo/kg) for 14 d. Steers were then housed in individual metabolism stalls for 3 d to determine DMI. Steers were then blocked by BW and DMI and randomly assigned within block to one of three treatments (n = 4 steers per treatment). Treatments consisted of: 1) Control (no supplemental Mo); 2) 5.0 mg Mo/kg DM from sodium molybdate dihydrate (Mo-diet), and 3) 1.5 mg Mo/l from sodium molybdate dihydrate delivered in the drinking water (Mo-water). After the 3d DMI determination period, total fecal and urine output was collected for 5 d. Dry matter intake and DM digestibility were similar across treatments. Data were analyzed using a mixed effects model (PROC MIXED, SAS) for a completely randomized block design. Apparent absorption of Cu was greater (P < 0.05) in Control and Mo-water steers when compared to Mo-diet steers. Apparent retention of Cu was greater (P < 0.05) in Control steers when compared to Mo-diet steers. Steers receiving Mo-water had a similar apparent retention of Cu when compared to Control and Mo-diet steers. By design, treatment was a significant (P < 0.01) source of variation for Mo intake. Control steers had lesser (P < 0.05) Mo intake when compared to Mo-diet and Mo-water supplemented steers. Apparent absorption and retention of Mo were greater (P < 0.05) in Mo-diet steers compared to Control and Mo-water steers. These data indicate that Mo metabolism and apparent absorption of Cu are different when Mo is supplemented in water relative to feed.

PSIV-10 Effects of chronic molybdenum exposure in drinking water or feed on molybdenum and copper status and production performance of gestating and lactating beef cattle consuming a low-quality forage diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 294-295
Author(s):  
Meghan P Thorndyke ◽  
Octavio Guimaraes ◽  
Matheus Medrado ◽  
Briana V Tangredi ◽  
Huey Yi Loh ◽  
...  
Keyword(s):  
Drinking Water ◽  
Block Design ◽  
Positive Control ◽  
Negative Control ◽  
Production Performance ◽  
Feed Water ◽  
Minimal Impact ◽  
Mineral Status ◽  
Randomized Block Design ◽  
Calf Performance

Abstract The objective of this experiment was to evaluate the effects of Mo source (feed or water) on performance and mineral status of cows and calves fed a forage-based diet for two years (DM basis: 6.6% CP; 0.14% S, 6.7 mg Cu/kg, 2.4 mg Mo/kg). Fifty-four cow-calf pairs were stratified by cow age, BW, and liver Cu and Mo status, and randomly assigned to one of six treatments. Treatments consisted of: 1) Negative control (NC; no supplemental Mo or Cu); 2) Positive control [PC: NC + Cu (3 mg Cu/kg diet DM from CuSO4·5H2O)]; 3) NC + 500 µg Mo/L from MoNa2O4·2H2O in drinking water (Mo 500-water); 4) NC + 1000 µg Mo/L of MoNa2O4·2H2O in drinking water (Mo 1000-water); 5) Mo 1000-water + 3 mg Cu/kg diet DM from CuSO4·5H2O (Mo 1000-water+Cu); and 6) NC + 3 mg Mo/kg diet DM from MoNa2O4·2H2O (3.0 Mo-diet). Cattle were allowed ad-libitum access to feed, water, and a protein supplement throughout the experiment. During the winter months animals were housed in three replicate pens per treatment and during the summer months animals were housed in separate pastures by treatment where cow and calf feed and water intake could be measured separately. Data were analyzed as a completely randomized block design. Throughout the experiment no signs of molybdenosis were observed. There was a treatment x time (P < 0.01) interaction for cow liver Cu concentrations. Cows not receiving supplemental Cu had lower (P < 0.01) liver Cu concentrations than cows receiving supplemental Cu. Cow and calf performance, calf mineral status, cow liver Mo, and plasma and milk Mo and Cu concentrations were similar across treatments. These data indicate that Mo supplemented in water or feed at concentrations used in this experiment had minimal impact on Cu status and overall animal performance.

PSVI-14 Blend of Additives in Nursery Pig Diets as an Antibiotics Substitute Increases the Growth Performance and Intestinal Health

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 201-201
Author(s):  
Sudario Roberto Silva Junior ◽  
Rhuan F Chaves ◽  
Maíra Resende ◽  
Ygor Henrique De Paula ◽  
Kenio De Gouvêa Cabral ◽  
...  
Keyword(s):  
Growth Performance ◽  
Animal Feed ◽  
Block Design ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Intestinal Health ◽  
Total Period ◽  
Nursery Pigs ◽  
Randomized Block Design ◽  
Diarrhea Incidence

Abstract Antibiotics growth promoting (AGP) in animal feed has been restricted. With this, several additives have been tested in order to replace AGP and ensure intestinal health. The objective of this study was to evaluate the effects of a blend of additives on growth performance, diarrhea incidence and cecal content microbiota of nursery pigs. A total of 576 weaned pigs (6.21 ± 1.16 kg) were assigned into 4 treatments (12 replicates) in randomized block design: basal diet without additives (NC); basal diet with 120 ppm of halquinol (PC); NC + 0,05% blend of lactic acid (3,5%), benzoic acid (2,5%), flavonoids (3,5%) (B5); NC+ 0,1% of same blend (B10). The pigs were weighed 0 and 42 days into the experiment. On day 10, one animal per pen (10 replicates) was euthanized for microbiota analyses. The diarrhea incidence was calculated by the percentage of visual pens with diarrhea, by day, in the period. The growth performance was analyzed by GLIMMIX procedure and the means were compared with Tukey test (P < 0.050) (SAS, 2009). Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The Kruskal Wallis test was used to compare microbial biodiversity (P < 0.050) by statistical program STAMP. The final weight (P = 0.010) and average daily gain (P = 0.001) of B10 and PC were higher than NC. Regarding diarrhea, it was observed that the B5 and B10 had lower incidence when compared to other groups in the total period. In the microbiome analyses, it was observed that the B5, B10, and PC groups had lower (P = 0.021) biodiversity when compared to the NC piglets. However, the B5 and B10 group had higher biodiversity when compared to the PC group. In conclusion, the supplementation with 0,1% blend of additives can be a substitute for AGP.

Long-term effects of feeding diets without mineral phosphorus supplementation on the performance and phosphorus excretion in high-yielding dairy cows

2007 ◽  
Vol 87 (4) ◽  
pp. 639-646 ◽  
Author(s):  
N. E. Odongo ◽  
D. McKnight ◽  
A. KoekKoek ◽  
J. W. Fisher ◽  
P. Sharpe ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Body Condition Score ◽  
Block Design ◽  
Holstein Cows ◽  
Long Term Effects ◽  
Total Mixed Ration ◽  
Mineral Phosphorus ◽  
Randomized Block Design ◽  
Randomized Design

The objective of this study was to assess the long-term effects of feeding a diet with no mineral phosphorus (P) supplementation on performance and P excretion in high-yielding dairy cows. In exp. 1, 24 primiparous (PP) and 40 multiparous (MP) Holstein cows were allocated to one of two treatments at calving: (1) regular corn silage and alfalfa haylage based milking cow total mixed ration (TMR; 0.42% P diet) or (2) milking cow total mixed ration (TMR) without supplemental mineral P (0.35% P diet) in a completely randomized design. The trial lasted until after two lactations were completed or the cow was culled. In exp. 2, eight MP Holstein cows (108 ± 8.0 d in milk) were used to determine P digestibility and retention in a completely randomized block design. In exp. 1, the 0.35% P diet cows had lower body weight, body condition score and milk urea nitrogen and higher faecal P than the 0.42% P cows. The dry matter intake (DMI) of PP cows on the 0.35% P diet was lower than that of PP cows on the 0.42% P diet. There was no difference in the DMI of MP cows. The P intake of 0.35% P diet PP cows was 30% lower than that of 0.42% P diet PP cows. The P intake of 0.35% P diet MP cows was 20% lower than that of 0.42% P diet MP. The digestibility coefficient of P for the 0.35% P diet was higher than that for the 0.42% P diet. These results suggest that the forages used in the present study contained adequate amounts of P to sustain milk production in high-yielding MP cows. However, for PP cows some mineral P supplementation is recommended especially during early lactation. Key words: Phosphorus, environmental pollution, intensive dairy operations

scholarly journals PENGARUH SUPLEMENTASI MINERAL NUTRIENT BLOCK (MNB) PADA PAKAN DASAR RUMPUT LAPANGAN TERHADAP PERFORMANS DAN KARKAS KELINCI JANTAN LOKAL (Lepus nigricollis)

2017 ◽  
Vol 20 (3) ◽  
pp. 106
Author(s):  
KUSUMA, I. G. P. E. ◽  
G. A. M KRISTINA DEWI ◽  
I M. NURIYASA
Keyword(s):  
Drinking Water ◽  
Block Design ◽  
Mineral Nutrient ◽  
Treatment Control ◽  
Male Rabbit ◽  
Randomized Block Design ◽  
Variable Performance

The research of the performance and carcass local male rabbit result mineral nutrient block (MNB)supplementation on ration-based grass field carried in Dajan Peken Village, Tabanan Regency (50 masl) formaintenance and cuts that lasted for 3 months. The design used in this research is a randomized block design, withfive replications. The rabbits were allocated randomly into four treatments, namely rabbits treated control rationis grass field (R0), control ration supplemented MNB 15 g/head/day (R1), control ration supplemented MNB 30 g/head/day (R2), control ration supplemented MNB 45 g/head/day (R3). Rations and drinking water given ad_libitum.Based on the results that the variable performance and carcass local male rabbits given MNB-supplemented rationswith 45 g/head/day (R3) showed a higher yield than R0 ration treatment (control) and other treatments.

A comparative study of non-protein nitrogen supplements for sheep

1976 ◽  
Vol 87 (3) ◽  
pp. 567-572 ◽  
Author(s):  
N. W. Offer ◽  
R. A. Evans ◽  
R. F. E. Axford
Keyword(s):  
Ammonium Acetate ◽  
Block Design ◽  
Basal Diet ◽  
Ammonia Nitrogen ◽  
Energetic Efficiency ◽  
Microbial Protein ◽  
Protein Nitrogen ◽  
Randomized Block Design ◽  
Ammonium Lactate ◽  
Gross Energy

SummaryA basal diet, or the basal diet supplemented with equi-nitrogenous amounts of either ammonium lactate, ammonium acetate or urea was fed to four re-entrant cannulated sheep in a randomized block design.Collections of duodenal digesta were carried out automatically for a continuous period of 4 days following a 10-day equilibration period for each sheep on each diet.Supplementation with non-protein nitrogen increased the passage of total amino acids to the duodenum and increased the proportion attributed to microbial protein. It improved pre-duodenal digestibility of modified acid-detergent fibre and increased post-abomasal digestibility of non-ammonia nitrogen, dry matter and gross energy.Supplementation with non-protein nitrogen increased the energetic efficiency of microbial protein production in the rumen.Ammonium lactate and acetate were safe and effective sources of non-protein nitrogen for dietary supplementation.Supplementation with ammonium lactate led to a greater passage of microbial protein to the duodenum than did equi-nitrogenous supplements of urea or ammonium acetate.

scholarly journals Standardized ileal digestible (SID) isoleucine requirement of barrows (15- to 30- kg) fed low crude protein diets

2017 ◽  
Vol 38 (5) ◽  
pp. 3283
Author(s):  
Doglas Batista Lazzeri ◽  
Leandro Dalcin Castilha ◽  
Patrícia Barcellos Costa ◽  
Ricardo Vianna Nunes ◽  
Magali Soares dos Santos Pozza ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Experimental Unit ◽  
Optimum Level ◽  
Initial Weight ◽  
Protein Deposition ◽  
Randomized Block Design ◽  
Protein Diets

This study aimed to determine the SID isoleucine (Ile) requirement of starting barrows fed low crude protein. Two experiments were carried out. Experiment 1: Ten crossbred barrows were used in order to determine the SID AA of the basal diet (treatment with the lowest SID Ile level used in the growth performance experiment), averaging 15.00 ± 0.27 kg of initial weight, individually housed in metabolic cages and allotted in a complete randomized design, with two treatments, five replicates and one animal per experimental unit. Treatments consisted of a basal (14.13% CP and 0.450% of SID Ile) and a free protein diet. Experiment 2: A performance experiment was carried out to determine the SID Ile requirement when using low crude protein diets. Forty crossbred barrows were used, averaging 15.00 ± 0.87 kg of initial weight and distributed in a randomized block design with five treatments (0.450, 0.520, 0.590, 0.660 and 0.730% of SID Ile) and two animals per experimental unit. The average daily gain (ADG) (P=0.049) and protein deposition (P=0.01) were affected by the studied SID Ile levels. The daily need of SID Ile was estimated at 5.9 g when considering 0.61% as the optimum level of SID Ile in the diet for an improved ADG and protein deposition.

scholarly journals Efisiensi penggunaan protein oleh babi yang mendapat pakan mengandung tepung temulawak (Curcuma xanthorrhiza)

2020 ◽  
Vol 7 (1) ◽  
pp. 55-62
Author(s):  
Ninengah Suryani ◽  
I Made Suaba Aryanta
Keyword(s):  
Body Weight ◽  
Protein Efficiency Ratio ◽  
Body Weight Gain ◽  
Block Design ◽  
Basal Diet ◽  
Efficiency Ratio ◽  
Protein Consumption ◽  
Randomized Block Design ◽  
Curcuma Xanthorrhiza ◽  
Average Body

The aimed of this study was to determine the effect of Curcuma Xanthorrhiza meal 0%, 0.5%, 0.75% and 1% on the efficiency of protein use (protein consumption, protein intake, body weight gain and protein efficiency ratio (REP). Twelve castration male pigs aged 3 months with an average body weight of 33.8 kg (CV = 23%). This study used a randomized block design (RBD) with four treatments and three replications. The treatment diets namely: R0 (100% basal diet without curcumin meal), R1 (basal diet + 0.5% curcumin meal), R2 (basal diet + 0.75%  curcumin meal and R3 (basal deat + 1% curcumin meal). The results showed that the addition up to 1% had no significant effect on ration consumption, protein consumption, protein utilization and protein efficiency ratio (PER) (P <0.05). It was concluded that the addition up to 1% curcuma meal could not increased protein efficiency. ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui efek penambahan tepung temulawak (Curcuma xanthorrhiza) 0%, 0,5%, 0,75% dan 1% dalam ransum terhadap efisiensi penggunaan protein: konsumsi protein, asupan protein, pertambahan bobot badan dan rasio efisiensi protein (REP) ternak babi.  Dua belas ekor babi jantan kastrasi umur 3 bulan dengan bobot badan rata-rata 33,8 kg (CV=23%).  Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan empat perlakuan dan tiga ulangan.  Perlakuan pakan terdiri dari : R0 (100 % ransum basal tanpa tepung temulawak), R1 (ransum basal + 0,5% tepung temulawak), R2 (ransum basal + 0,75% tepung temulawak) dan R3 ransum basal + 1% tepung temulawak).  Hasil penelitian menunjukkan penambahan tepung temulawak sampai 1% berpengaruh tidak nyata terhadap konsumsi ransum, konsumsi protein, asupan protein  dan Rasio Efisiensi Penggunaan Protein (REP) (P>0,05).  Disimpulkan bahwa penambahan tepung temulawak sampai1% belum mampu meningkatkan efisiensi penggunaan protein oleh ternak babi fase pertumbuhan.

PSIII-32 The influence of liver copper concentration on copper homeostatic liver proteins in beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 258-259
Author(s):  
Nicole M Tillquist ◽  
Meghan P Thorndyke ◽  
Terry Engle
Keyword(s):  
Superoxide Dismutase ◽  
Beef Cattle ◽  
Relative Abundance ◽  
Protein Function ◽  
Block Design ◽  
Basal Diet ◽  
Beef Cows ◽  
Glutathione Synthetase ◽  
Flavin Reductase ◽  
Minimal Impact

Abstract The objective of this experiment was to investigate the influence of liver Cu concentrations on the relative abundance of liver Cu homeostatic proteins in beef cattle. Archived liver biopsy samples were selected based on Cu concentrations (n = 4 samples 21.7±1.35 mg Cu/kg DM-deficient; and n = 4 samples 73.3 ±10.7 mg Cu/kg DM-adequate). Liver samples were obtained from a subset of multiparous beef cows receiving a forage-based diet with no supplemental Cu (basal diet 6.25 mg Cu/kg DM) or 10 mg Cu/kg DM total diet (Cu supplemented as CuSO4·5H2O) for 99 d. Liver proteins were identified using mass spectrometry, normalized, and relative abundance determined using Scaffold software. A total of 895 identical proteins were identified in each sample and relative abundance of each Cu specific homeostatic protein (n = 13) was recorded. Data were analyzed as a randomized complete block design using R software. Copper homeostatic liver proteins identified were: aldehyde dehydrogenase, apolipoprotein A-1, betaine homocysteine methyltransferase, carbonic anhydrase II, Cu chaperone for superoxide dismutase, Cu transport protein, cytochrome c oxidase Cu chaperone, extracellular superoxide dismutase, flavin reductase, glutamate dehydrogenase, glutathione synthetase, protein disulphide isomerase A3, and Cu-zinc superoxide dismutase. By design, liver Cu concentrations were greater (P &lt; 0.05) in Cu adequate vs. Cu deficient liver samples. Copper deficient liver samples had greater (P &lt; 0.05) relative abundance of glutathione synthetase compared to Cu adequate liver samples. The relative abundance of all other Cu homeostatic liver proteins identified were similar (P &gt; 0.05) across Cu concentrations. These data suggest that deficient and adequate liver Cu concentrations ranging from 16.0 to 109.0 mg Cu/kg DM have minimal impact on the relative abundance of Cu homeostatic proteins in beef cattle. Further investigation is needed to determine if liver Cu concentration influences Cu homeostatic protein function.

scholarly journals 76 Does supplemental protein and rumen-protected methionine improve performance and digestibility during late-gestation in beef cows?

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 75-75
Author(s):  
Madeline M Collins ◽  
Michaela K S Lievre ◽  
Kaitlyn V J Lawson ◽  
Ira B Mandell ◽  
Anna-Kate Shoveller ◽  
...  
Keyword(s):  
Body Weight ◽  
Amino Acid ◽  
Block Design ◽  
Dietary Treatment ◽  
Beef Cows ◽  
Late Gestation ◽  
Isoleucine Leucine ◽  
Randomized Block Design ◽  
Cow Performance ◽  
Supplemental Protein

Abstract Methionine (MET) is likely the first limiting amino acid in low-protein forages fed to beef cattle during late-gestation. Therefore, the objective of this study was to determine if supplemental protein and rumen-protected MET improve cow performance and apparent tract digestibility (TTD) during late-gestation. This study used 147 late-gestation Angus crossbred cows and heifers in a 3x2 factorial arrangement for dietary treatments. The cattle were randomly assigned to one of six diets formulated to 90, 100 or 110% of metabolizable protein (MP) requirements (NRC, 2016), with (without) 9 g/d of rumen-protected MET (MetaSmart, Adisseo Inc.). These diets were fed for approximately 8 wks before calving. All data was analyzed as a randomized block design using SAS 9.4 PROC GLIMMIX procedure. Cows fed at 90% MP requirements lost body weight (BW), while cows fed at 100% and 110% MP requirements maintained and (or) gained BW over the trial (P = 0.02). Similarly, cows fed at 90% MP requirements lost more pregnancy corrected BW than cows fed at 100% and 110% MP requirements (P = 0.01). However, supplemental MET did not affect body weight gains (P &gt; 0.07). Cows and heifers fed at 90% MP requirements had reduced TTD for crude protein compared to cattle fed to 110% MP requirements (P &lt; 0.001), MET supplementation did not impact TTD (P &gt; 0.20). Additionally, cattle fed at 90% MP requirements had increased serum cholesterol and reduced urea concentrations compared to cows fed to 100% and 110% MP requirements (P &lt; 0.0001). MET supplementation increased serum concentrations for glucose, isoleucine, leucine, lysine, serine, threonine and valine (P &lt; 0.02). Calf birth weights were not significantly impacted by dietary treatment (P &gt; 0.31). Feeding cows above their MP requirements may improve late-gestation performance and CP digestibility. Supplemental MET may increase amino acid utilization but did not improve beef cow performance or digestibility parameters measured in late-gestation.

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