PSVII-25 Intra-Farm Variability: Growing pigs have different SID Lys:NE requirements depending on their BW category

Abstract The objective of this experiment was to determine if pigs with different initial body weight within the same farm batch have different SID Lys:NE requirements. In the trial 1,170 growing crossbred pigs were distributed in 90 pens (13 pigs/pen) and split in three initial BW categories: Large, Medium and Small (32.1 ± 2.8, 27.5 ± 2.3 and 23.4 ± 2.9 kg, respectively). Each pen was randomly assigned by BW to one of the SID Lys:NE levels (3.25, 3.66, 4.07, 4.47 and 4.88 g SID Lys/Mcal NE), with 6 replicates per dietary treatment. All diets were isoenergetic (2460 kcal NE/kg), and CP ranged from 14.50 to 17.50 %. The dietary treatments were fed for 26 days. Statistical analysis to determine the effect of SID Lys:NE was performed using the GLM procedure of SAS with linear and quadratic polynomial contrasts. In addition, broken-line linear models were fit with the NLIN procedure of SAS to determine the break-point to maximize ADG or minimize FCR for all categories together or independently. The ADG break-point for all pigs was determined at 3.89 g SID Lys/Mcal NE. However, when analyzed by categories, the break-point was 3.86, 4.07 and 4.76 g SID Lys/Mcal NE for large, medium and small pigs respectively. The general break-point for FCR was determined at 4.25 g SID Lys/Mcal NE. By BW categories the different break points determined were: 4.27, 4.15 and 4.59 g SID Lys/Mcal NE for large, medium and small pigs, respectively. In conclusion, the estimated requirement to maximize ADG and optimize FCR via broken-line models is higher for small pigs than large or medium. Therefore, pigs within the same farm batch should be differentially fed in relation to the SID Lys:NE ratio.

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PSXII-29 Utilizing coproducts as an alternative to forage in adapting feedlot cattle to finishing diets affects the ruminal microbiome

Journal of Animal Science ◽

10.1093/jas/skz258.826 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 417-417

Author(s):

David M Crawford ◽

Joshua C McCann

Keyword(s):

Body Weight ◽

Microbial Community ◽

Alpha Diversity ◽

Dietary Treatment ◽

Feedlot Cattle ◽

Distillers Grains ◽

Adaptation Period ◽

Initial Body Weight ◽

Finishing Diets ◽

Dietary Treatments

Abstract The objective was to determine the interaction of replacing forage with coproducts and the number of step-up diets when adapting feedlot cattle to a finishing diet on the ruminal microbiome. Simmental × Angus and Angus steers (n = 24; 234 ± 38 kg) were blocked by initial body weight (BW) and allotted to 8 pens as a subset of a larger group. Pens were randomly assigned to 1 of 2 dietary treatments: 1) decreasing coproduct (CO; soybean hulls and modified wet distillers grains) inclusion while increasing dry-rolled corn over 36 d or 2) decreasing forage (FO; alfalfa and grass hay) inclusion while increasing dry-rolled corn over 36 d. Pens were fed either 5 (5S) or 2 (2S) step-up diets for each dietary treatment during the 36 d adaptation period in a 2 × 2 factorial design. Steers were fed a common finishing diet for the remainder of the trial (34 d). The ruminal microbiome was evaluated on d 36 and 70. Alpha diversity was greatest (P < 0.01) for FO/2S steers on d 36. A diet × day effect (P = 0.02) was observed for beta diversity as the microbial community was more similar (P < 0.01) between d 36 and 70 for steers fed CO compared with FO. Relative abundance of Bacteroidetes was greater (P = 0.03) for 2S steers compared with 5S steers. Prevotellaceae tended to be greater (P = 0.07) in 2S steers compared with 5S steers. Lactobacillaceae was greater (P < 0.01) on d 36 than d 70. Limited observed day effects for individual taxa suggest the microbiome was well adapted by treatments for the finishing diet on d 36. Greater coproduct inclusion in adaptation diets may alter the ruminal bacterial community to be more similar to the community while feeding high levels of dry-rolled corn.

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The influence of sodium supplementation of two phosphorus sources on performance and bone mineralization of growing-finishing swine evaluated at two geographical locations

Canadian Journal of Animal Science ◽

10.4141/cjas91-064 ◽

1991 ◽

Vol 71 (2) ◽

pp. 537-547 ◽

Cited By ~ 1

Author(s):

E. T. Kornegay ◽

M. D. Lindemann ◽

H. S. Bartlett

Keyword(s):

Drinking Water ◽

Body Weight ◽

Growth Performance ◽

Bone Mineralization ◽

Growing Pigs ◽

Dietary Treatments ◽

Sodium Phosphates ◽

Mg Content ◽

Crossbred Pigs ◽

Geographical Locations

Crossbred pigs (n = 144) were used at two geographical locations (mountain and coastal) to evaluate the effects of varied Na intake on growth performance, serum minerals and bone characteristics of growing-finishing swine (average initial body weight 24.5 kg) fed either defluorinated phosphate (DFP) which contained 5% Na or dicalcium phosphate (DCP) which contained < 0.1% Na. The six dietary treatments were DFP with 0, 0.07, 0.14 and 0.28% added Na and DCP with 0 and 0.14% added Na. The basal corn-soybean meal diet contained 0.01% Na, and the Cl content was held constant at 0.25% in all diets. Drinking water at the mountain location contained 4 mg L−1 Na and 194 mg L−1 Na at the coastal location. Growth performance did not differ among treatments, with the exception of pigs fed the DCP diet without added Na (total Na, 0.01%) at the mountain location which had depressed daily gain, daily feed intake and feed per gain compared with all other diets. When metacarpals (MC) and metatarsals (MT) taken from barrows at slaughter were examined on a body weight-corrected basis, Na intake and P source did not consistently influence bone dimensional and strength characteristics. Pigs fed the DCP diet without added Na at the mountain location had a lower Na and higher Mg content of MC and MT, and serum Na and Ca concentrations were reduced while serum P, Mg and K concentrations were unchanged. Varying Na intake did not influence bone strength and mineralization, and the Na in defluorinated phosphate and drinking water is readily available for growing pigs. Key words: Sodium, phosphates, bone mineralization, pigs

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Digestibility of amino acids in pigs fed distillers dried grains with solubles derived from corn, sorghum, and triticale

Canadian Journal of Animal Science ◽

10.1139/cjas-2020-0076 ◽

2020 ◽

Author(s):

Chan Sol Park ◽

Darryl Ragland ◽

O. Adeola

Keyword(s):

Amino Acids ◽

Body Weight ◽

Latin Square ◽

Experimental Period ◽

Growing Pigs ◽

Initial Body Weight ◽

Isoleucine Leucine ◽

Ileal Digestibility ◽

Distillers Dried Grains ◽

Dietary Treatments

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows (initial body weight = 23.1 ± 2.03 kg) surgically fitted with T-cannulas at the distal ileum were divided into 4 groups based on body weight and assigned to a quadruplicated 4 × 2 incomplete Latin Square design with 4 dietary treatments and 2 experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS and a nitrogen-free diet were fed in each experimental period consisting of 5-d adaptation and 2-d ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed from pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.

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171 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine on Nitrogen Balance of Growing Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.102 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 61-61

Author(s):

Woongbi Bi Kwon ◽

Jose A Soto ◽

Hans H Stein

Keyword(s):

Body Weight ◽

Crude Protein ◽

Random Effect ◽

Basal Diet ◽

Growing Pigs ◽

Negative Effects ◽

Initial Body Weight ◽

N Retention ◽

Biological Value ◽

Main Effects

Abstract An experiment was conducted to test the hypothesis that increasing concentrations of dietary Val, Ile, or Trp alone or in combination will alleviate negative effects of excess dietary Leu on N balance of growing pigs. Seventy-two barrows (initial body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly assigned to 1 of 8 diets and 3 blocks with 3 pigs per diet in each block in a 12-d experiment. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of L-Val (0 or 0.1%), L-Ile (0 or 0.1%), and L-Trp (0 or 0.05%) that were added to a basal diet containing corn and a high-protein corn product (48% crude protein; 5.9% Leu). The basal diet contained 1.00 % standardized ileal digestible (SID) Lys and 171% SID Leu:Lys. Data were analyzed using the PROC MIXED of SAS with concentrations of Val, Ile, and Trp, and all interactions as main effects and replicate as a random effect. No 3-way interactions were observed (Table 1). Results indicated that fecal N output increased if Ile was added to diets without added Val, but that was not the case if Val was added (interaction, P < 0.05). Addition of Ile to diets reduced N retention, but N retention increased with Trp addition to diets without Val addition, but not if Trp was added to diets with added Val (interaction, P < 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P < 0.05). In conclusion, adding Ile to a diet with excess Leu reduced N retention, but if Trp was added alone or in combination with Ile or Val, N retention increased.

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Standardized ileal digestible amino acids and net energy contents in full fat and defatted black soldier fly larvae meals (Hermetia illucens) fed to growing pigs

Translational Animal Science ◽

10.1093/tas/txaa104 ◽

2020 ◽

Vol 4 (3) ◽

Author(s):

Michelina Crosbie ◽

Cuilan Zhu ◽

Anna K Shoveller ◽

Lee-Anne Huber

Keyword(s):

Amino Acids ◽

Body Weight ◽

Latin Square ◽

Growing Pigs ◽

Net Energy ◽

Initial Body Weight ◽

Black Soldier Fly ◽

Digestible Energy ◽

Black Soldier Fly Larvae ◽

Fly Larvae

Abstract Two experiments were conducted to determine standardized ileal digestibility (SID) of amino acids (AA; Exp. 1) and net energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) samples [full fat (FF; 42.5% crude protein (CP), as-fed) and defatted (DF; 40.8% CP; as-fed)] for growing pigs. Two cornstarch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used, and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy (DE) by difference method. In each experiment, pigs were fed 2.8 × estimated maintenance energy requirement. In Exp. 1, eight ileal-cannulated barrows (25.1 ± 0.41 kg initial body weight) were used in a replicated 2 × 2 Latin square design (n = 8). In each period, pigs were adapted to diets for 5 d followed by 2 d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, eight barrows [23.4 ± 0.54 kg initial body weight (BW)] were used in a partially replicated Latin square design (n = 8). In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The SID of CP (80.6 ± 1.1%) and Lys (88.0 ± 1.4%) were not different between FF and DF BSFLM. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P = 0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible energy (4,927 vs. 3,941 ± 75 kcal/kg), metabolizable energy (4,569 vs. 3,396 ± 102 kcal/kg), and predicted net energy (3,477 vs. 2,640 ± 30 kcal/kg, using equations from Noblet; 3,479 vs. 2,287 ± 28 kcal/kg, using equations from Blok, respectively) were greater for the FF versus the DF BSFLM (P < 0.05). The apparent total tract digestibility of neutral detergent fiber and acid detergent fiber were greater for the FF versus the DF BSFLM (P ≤ 0.05). Both FF and DF BSFLM had high SID for most AA; however, FF BSFLM was a better source of net energy for growing pigs. Therefore, both FF and DF BSFLM could be used as protein alternatives in growing pig diets.

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Energy, amino acid, and phosphorus digestibility and energy prediction of thermally processed food waste sources for swine1

Translational Animal Science ◽

10.1093/tas/txz028 ◽

2019 ◽

Vol 3 (2) ◽

pp. 676-691 ◽

Cited By ~ 1

Author(s):

Leonard Fung ◽

Pedro E Urriola ◽

Gerald C Shurson

Keyword(s):

Amino Acids ◽

Body Weight ◽

Food Waste ◽

Block Design ◽

Growing Pigs ◽

In Vitro Digestibility ◽

Prediction Equations ◽

Initial Body Weight

Abstract Recycling energy and nutrients from food waste into animal feed decreases the environmental impact of food animal production. However, recycling energy and nutrients from various food waste sources into swine feeding programs is constrained by the high variability and lack of data on the digestibility of energy and nutrients. Therefore, the objectives of this study were to evaluate the digestibility of energy, amino acids, and phosphorus in thermally dried food waste sources fed to growing pigs and to compare in vivo determined digestibility values with those obtained from in vitro digestibility procedures and published prediction equations to determine the accuracy of using these nutritional evaluation methods. Pigs (n = 36; initial body weight = 16.37 ± 1.9 kg) were utilized to determine digestible energy (DE) and metabolizable energy (ME) content, as well as standardized total tract digestibility (STTD) of phosphorus and standardized ileal digestibility (SID) of amino acids in three sources of dehydrated food waste in three separate trials. Initial body weight of pigs at the beginning of each digestibility trial was used as the blocking factor in a randomized complete block design. Diets were formulated to contain 30% food waste derived from fish waste (FW), supermarket waste (containing bakery, fruits and vegetables, meat, and deli foods from a single supermarket; SMW), and fruit and vegetable waste (FVW). The DE and ME content of FW (DE = 5,057 kcal/kg; ME = 4,820 kcal/kg) and SMW (DE = 5,071 kcal/kg; ME = 4,922 kcal/kg) were not different (P > 0.05), whereas FVW had the least (P < 0.05) DE (2,570 kcal/kg) and ME (2,460 kcal/kg) content compared with FW and SMW. Digestibility of crude protein and amino acids was greater (P < 0.05) in FW and SMW compared with FVW. The in vitro digestibility procedure can be used to approximate the digestibility of dry matter (DM) and energy in SMW, FW, and FVW compared with in vivo estimates, but significant error exists depending on the chemical characteristics of each food waste source. However, use of the prediction equations and digestibility data obtained from the in vitro procedure resulted in high accuracy in estimating DE content of FW (observed = 5,058 kcal/kg DM vs. predicted = 4,948 kcal/kg DM), SMW (observed = 5,071 kcal/kg DM vs. predicted 4,978 kcal/kg DM), and FVW (observed = 2,570 kcal/kg DM vs. predicted 2,814 kcal/kg DM) sources.

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Development and Validation of Equations for Predicting the Metabolizable Energy Value of Double-Low Rapeseed Cake for Growing Pigs

Animals ◽

10.3390/ani11041168 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1168

Author(s):

Lu Wang ◽

Qile Hu ◽

Peili Li ◽

Changhua Lai ◽

Defa Li ◽

...

Keyword(s):

Body Weight ◽

Soybean Meal ◽

Block Design ◽

Basal Diet ◽

Prediction Equation ◽

Growing Pigs ◽

Metabolizable Energy ◽

Neutral Detergent Fiber ◽

Chemical Compositions ◽

Initial Body Weight

The study was conducted to develop and validate an equation to predict the metabolizable energy (ME) of double-low rapeseed cakes (DLRSC) for growing pigs based on their chemical compositions. In Experiment 1, 66 growing pigs (initial body weight 36.6 ± 4.1 kg) were allotted randomly to a completely randomized design with 11 diets. The diets included a corn–soybean meal basal diet and 10 test diets containing 19.22% DLRSC supplemented at the expense of corn, soybean meal, and lysine. Neutral detergent fiber (NDF), crude fiber (CF), and gross energy (GE) were the best predictors to determine ME. The best-fit prediction equation of ME (MJ/kg) was ME = 9.33 − 0.09 × NDF − 0.25 × CF + 0.59 × GE (R2 = 0.93). In Experiment 2, a total of 144 growing pigs (initial body weight 29.7 ± 2.7 kg), with six pigs per pen and six pens per treatment, were assigned randomly to four treatments in a completely randomized block design for a 28-day feeding trial. A corn–soybean meal basal diet was prepared, and three additional diets were formulated by adding 7%, 14%, and 21% DLRSC to the basal diet at the expense of soybean meal. All diets were formulated to provide equal standardized ileal digestibility (SID) Lys/ME ratio and SID essential amino acids/SID Lys ratio. Increasing dietary levels of DLRSC had no effect on average daily feed intake, average daily gain, and feed-to-gain ratio. The caloric efficiency of ME (31.83, 32.44, 31.95, and 32.69 MJ/kg, respectively) was not changed by increasing the dietary concentration of DLRSC. Increasing dietary levels of DLRSC linearly reduced (p < 0.05) the concentrations of triiodothyronine and tetraiodothyronine in serum, as well as apparent total tract digestibility of DM, GE, crude protein, acid detergent fiber, and organic matter of the diet. In conclusion, the ME prediction equation obtained in Experiment 1 accurately estimates the ME value of DLRSC fed to growing pigs.

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PSXI-28 Reducing dietary crude protein and supplementation with amino acids helps mitigate the environmental impacts of growing pigs production determined by the life cycle assessment

Journal of Animal Science ◽

10.1093/jas/skz258.763 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 383-383

Author(s):

Lucas Antonio C Esteves ◽

Natália Y Sitanaka ◽

Alessandra N T R Monteiro ◽

Suelen M Einsfeld ◽

Lucas P Bonagurio ◽

...

Keyword(s):

Amino Acids ◽

Body Weight ◽

Life Cycle Assessment ◽

Environmental Impacts ◽

Crude Protein ◽

Block Design ◽

Growing Pigs ◽

Experimental Unit ◽

Initial Body Weight ◽

Randomized Block Design

Abstract This study aimed to evaluate, through life cycle assessment and using experimental data, the environmental impacts of growing pigs’ production (30 to 50 kg) fed diets with reduced crude protein (CP) levels, and supplemented with industrial amino acids. Experiment I (nitrogen and phosphorus balance): 20 crossbred barrows, averaging 31.80 ± 2.39 kg of initial body weight, were housed in metabolic cages and allotted in a randomized block design with four treatments, five replicates and one animal per experimental unit. Experiment II (performance): 44 crossbred barrows, averaging 30.10 ± 0.63 kg of initial body weight, were housed in a randomized block design, with four treatments, 11 replicates and one animal per experimental unit. The treatments for both experiments consisted of four diets containing 18.15, 17.15, 16.15 and 15.15% of CP, and supplemented with industrial amino acids, in order to meet the pig’s requirements of SID amino acids. According to the obtained data (Experiments I and II), the environmental impacts for global warming potential (GWP), acidification potential (AC), eutrophication potential (EU), cumulative energy demand (CED), terrestrial ecotoxicity (TE) and land occupation (LO), per kg of body weight gain, were calculated (CML 2001 method). There was a reduction in some studied environmental impacts as the dietary CP reduced from 18.15 to 15.15%, observing a reduction in the AC from 35.34 to 31.58 g SO2-eq (P = 0.015), there was also a reduction in the EU from 11.90 to 10.31 g PO4-eq (P = 0.001), and the LO was reduced from 2.15 to 1.89 m2-year (P = 0.005), respectively. GWP, CED and TE were not affected (P > 0.05) by the dietary CP reduction, although some variations were observed. It was concluded that the dietary CP reduction, supplementing with industrial amino acids, may help to mitigate the environmental impacts caused by growing pig production for the AC, EU and LO categories.

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THE SIGNIFICANCE OF THE REDUCED FEED CONSUMPTION OBSERVED IN GROWING PIGS FED VOMITOXIN-CONTAMINATED DIETS

Canadian Journal of Animal Science ◽

10.4141/cjas86-028 ◽

1986 ◽

Vol 66 (1) ◽

pp. 277-287 ◽

Cited By ~ 10

Author(s):

E. R. CHAVEZ ◽

J. A. RHEAUME

Keyword(s):

Body Weight ◽

Control Diet ◽

Total Body Weight ◽

Growing Pigs ◽

Feed Consumption ◽

Control Analysis ◽

Weight Gains ◽

Ad Libitum ◽

Total Body ◽

Dietary Treatments

Feeding trials were conducted to determine the significance of the reduction in feed consumption observed when growing pigs were fed complete diets containing approximately 6 ppm vomitoxin. Sixty Landrace weaners, weighing on average 22 kg, were randomly assigned to four dietary treatments. The dietary treatments were: (1) control cereal soybean diet fed ad libitum (mycotoxin free); (2) vomitoxin diet fed ad libitum in which 100% of the clean wheat included in the control diet was replaced by moldy wheat containing 10.32 ppm vomitoxin; (3) control diet fed restricted to the average daily intake of pigs fed the vomitoxin diet; and (4) upgraded vomitoxin diet fed ad libitum and containing approximately 20% more energy, crude protein, minerals and vitamins. Individual feed consumption was recorded daily for 12 wk. The control diet promoted significantly (P < 0.05) greater total body weight gains and also the greatest feed consumption. Body weight gains were not different (P > 0.05) for the vomitoxin and restricted control dietary treatments. The vomitoxin upgraded treatment resulted in greater total body weight gains (P < 0.05) than the vomitoxin treatment. During the 12-wk period, animals in the vomitoxin and restricted-control-fed groups consumed 71% as much feed as the control group fed ad libitum while the vomitoxin upgraded group consumed 77% as much as the control. Analysis for blood urea nitrogen, creatinine, plasma protein and alkaline phosphatase revealed no differences (P > 0.05) between dietary treatments. With respect to animal performance and blood biochemical profiles, the effect of vomitoxin appears to be primarily limited to feed acceptability and intake, with no apparent consequences on the metabolic function of the animal. Key words: Vomitoxin, deoxynivalenol, growing pigs, feed consumption

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Effects of graded concentrations of supplemental selenium on selenium concentrations in tissues and prediction equations for estimating dietary selenium intake in pigs

PeerJ ◽

10.7717/peerj.5791 ◽

2018 ◽

Vol 6 ◽

pp. e5791 ◽

Cited By ~ 1

Author(s):

Ah Reum Son ◽

Jin-young Jeong ◽

Kyu Ree Park ◽

Minseok Kim ◽

Sung Dae Lee ◽

...

Keyword(s):

Body Weight ◽

Relative Weight ◽

Urine Samples ◽

Initial Body Weight ◽

Final Body Weight ◽

Blood Samples ◽

Dietary Selenium ◽

Independent Variable ◽

Quadratic Effects ◽

Dietary Treatments

The experiment was conducted to determine the effects of graded dietary selenium (Se) on organ weight and Se concentrations in tissues and to develop equations for estimating dietary Se intake in pigs. Sixteen barrows (initial body weight = 30.0 ± 2.6) were allotted to four dietary treatments including graded Se supplementations with 0, 1, 5, and 50 mg/kg of diet. The experimental diets fed to the pigs for 30 d, and then the pigs were euthanized, and the organs, muscle, and urine samples were collected. The hair and blood samples of pigs were collected on d 15 and 30. Equations were developed for predicting daily Se intake using the Se concentration in plasma, hair, liver, kidneys, muscle, or urine. For graded dietary Se concentrations, linear and quadratic effects on the final body weight, weight and relative weight of liver and kidneys were not observed. The Se concentration in plasma, hair, liver, kidneys, muscle, and urine were linearly and quadratically increased as dietary Se concentration increased (P < 0.001). The dietary Se concentration was positively correlated with the Se concentrations in the plasma, organs, muscle, and urine (r > 0.81,P < 0.001). The equations for estimating dietary Se intake using the Se concentration in the plasma, hair, or organ as an independent variable were significant (P < 0.05). In conclusion, the dietary Se concentration was well reflected in the Se concentration in the plasma, hair, liver, kidneys, and urine. The Se concentration in the plasma, hair, liver, and kidneys can be used as an independent variable for estimating the Se intake.

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