scholarly journals P1199THE IMPORTANCE OF DETERMINING ALPHA-2-MACROGLOBULIN CONCENTRATITON IN PATIENTS ON PERITONEAL DIALYSIS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Marko Baralic ◽  
Ana Bontic ◽  
Jelena Pavlovic ◽  
Mirjana Lausevic ◽  
Selena Gajic ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Covalent Binding ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Simple Method ◽  
Partial Denaturation ◽  
The Difference ◽  
And Function ◽  
Relationship Of

Abstract Background and Aims To determine the concentration of Alpha-2-macroglobulin (α2M) and its activity in patients treated with peritoneal dialysis (PD) and healthy subjects (H). Method a cross-sectional study included 50 PD and 30 H, in which the concentration of active α2M molecules was determined. The measurement is based on covalent binding of α2M and trypsin and zymography with gelatin incorporated into the electrophoretic gel. Results The intensity of proteolytic bands seen with native α2M was analyzed in relation to the concentration of α2M in patients with PD with less and / or more than three peritonitis. Linear dependence was only visible in the case of the upper range, which originates from the intact α2M-trypsin complex. The highest coefficient of variation is calculated for the smallest α2M concentration (4.2%). The signal intensities originating from α2M (H) were higher compared to PD, with the PD group being lower in patients with higher peritonitis, and the difference was more pronounced as concentration increased, resulting in a lower slope of dependence. The proposed method can reliably measure the amount of α2M originating in a group H, while the quantification of α2M in PD groups that may have structurally altered α2M can be seen more for condition estimation than for accurate measurements. The zymographic test in this experiment allows us to investigate the relationship of the concentration, structure and function of α2M in different pathological conditions, as well as in the presence of modifying agents that can accompany them. Conclusion A simple method for the determination of α2M concentration and function by zymography has been developed, which can be used to examine residual α2M activity after partial denaturation or structural modification due to disease.

scholarly journals Establishment of a new relationship between posed smile width and lower facial height: A cross-sectional study

2015 ◽  
Vol 09 (03) ◽  
pp. 394-399 ◽  
Author(s):  
Aby Abraham ◽  
Jinu George ◽  
Elbe Peter ◽  
Koshi Philip ◽  
Rajesh Chankramath ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Facial Height ◽  
The Face ◽  
Facial Proportions ◽  
Males And Females ◽  
The Mean ◽  
The Difference

ABSTRACT Objective: The present study is intended to add a new parameter that would be useful in orthodontic clinical evaluation, treatment planning, and determination of vertical dimension (at occlusion). Materials and Methods: Standardized videographic recording of 79 subjects during posed smile was captured. Each video was then cut into 30 photos using the free studio software. The widest commissure-to-commissure posed smile frame (posed smile width [SW]) was selected as one of 10 or more frames showing an identical smile. Lower third of the face is measured from subnasale to soft tissue menton using a digital vernier caliper. Two values were then compared. Ratio between lower facial height and posed SW was calculated. Results: The co-relation between smiling width and lower facial height was found to be statistically significant (P < 0.01). The ratio of lower facial height and smiling width was calculated as 1.0016 with a standard deviation (SD) = 0.04 in males and 1.0301 with an SD = 0.07 in females. The difference between the mean lower facial height in males and females was statistically significant with a t = 10.231 and P = 0.000. The difference between the mean smiling width in males and females was also statistically significant with a t = 5.653 and P = 0.000. Conclusion: In class I subjects with pleasing appearance, normal facial proportions, normal overjet and overbite, and average Frankfort mandibular angle, the lower facial height (subnasale to soft tissue menton) is equal to posed SW.

Relationship of Serum Cystatin C to Peritoneal and Renal Clearance Measures in Peritoneal Dialysis: A Cross-sectional Study

2008 ◽  
Vol 51 (2) ◽  
pp. 278-284 ◽  
Author(s):  
Michael P. Delaney ◽  
Paul E. Stevens ◽  
Mohammed Al Hasani ◽  
Helen J. Stowe ◽  
Caroline Judge ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Renal Clearance ◽  
Cystatin C ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Serum Cystatin C ◽  
Cross Sectional ◽  
Serum Cystatin ◽  
Relationship Of

scholarly journals Korelasi Konflik Kerja Dengan Tingkatan Stres Kerja Pada Perawat Di Rumah Sakit Umum Daerah Petala Bumi Provinsi Riau Tahun 2019

2020 ◽  
Vol 10 (2) ◽  
pp. 158-166
Author(s):  
Trisna Jayati
Keyword(s):  
Work Stress ◽  
Cross Sectional Study ◽  
P Value ◽  
Cross Sectional ◽  
Work Conflict ◽  
Differences Of Opinion ◽  
The Difference ◽  
Intensive Approach ◽  
Relationship Of

Prolonged stress can have an impact on reducing concentration, nurses become irritable with patients, increase absenteeism, disrupt sleep patterns, and reduce the quality of work by providing nursing care to patients. The purpose of this study was to determine the relationship of work conflict with the level of work stress on nurses at Petala Bumi Regional General Hospital, Riau Province in 2019. The type of research used was observational with analytic cross-sectional study design. The sample in this study was 101 nurses. Data analysis was performed univariate, bivariate and multivariate. The results of bivariate research variables work conflict related to the level of work stress are differences of opinion (p value = 0.002), misunderstanding (p value = 0.004), feeling disadvantaged (p value = 0.004) and feeling sensitive (p value = 0.004). Variables that have a causal relationship with work stress are differences of opinion, misunderstanding, feeling disadvantaged, sensitive feelings. The conclusion in this study is that there is a causal / multivariate relationship between differences of opinion, misunderstanding, feeling disadvantaged, feeling sensitive with an increase in work stress on nurses. It is recommended to the hospital, especially hospital management to take an intensive approach to nurses and supervise so that the source of the difference can be minimized and work that cannot be completed which can lead to disputes that cause stress.

scholarly journals Determination of Ki-67 expression in oral leukoplakia in snuff users and non-users in Khyber Pakhtunkhwa province of Pakistan.

2020 ◽  
Vol 27 (04) ◽  
pp. 682-687 ◽  
Author(s):  
Tehmina Naushin ◽  
Muhammad Mumtaz Khan ◽  
Sajjad Ahmed ◽  
Mahmood-ul- Hassan ◽  
Fatima Iqbal ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Oral Leukoplakia ◽  
P Value ◽  
Ki 67 ◽  
Cross Sectional ◽  
Khyber Pakhtunkhwa ◽  
Khyber Pakhtunkhwa Province ◽  
Relationship Of ◽  
The Relationship

Objectives: The aim of the article was to evaluate Ki-67 expression in oral leukoplakia in snuff users and non-users. Study Design: Descriptive Cross sectional study. Setting: At different hospitals of Khyber Pakhtunkhwa province. Period: From August 2016 –March 2017. Material & Methods: Clinically diagnosed and histopathologically confirmed cases of oral leukoplakia in snuff users and non-users 30 cases each were immunohistochemically examined and percentage expression of nuclear Ki-67 was evaluated by a semi quantitative method in basal, parabasal and suprabasal layers of the lining epithelium. Results: The relationship of expression of Ki-67 in parabasal and suprabasal layers of affected epithelium in snuff users was found to be statistically significant with a p value of 0.007 and 0.002 respectively. Conclusion: The present study concludes that the proliferative index is high in snuff users as compared to non-users which can be a factor for malignant transformation.

scholarly journals Determination of Relationship of Maxillary Occlusal Plane with Ala-Tragus Line: A Customised Approach in Aryan and Mongolian Ethnicity.

2019 ◽  
Vol 8 (2) ◽  
pp. 47-52
Author(s):  
Pawan Mehta ◽  
Shanti Chhetri
Keyword(s):  
Cross Sectional Study ◽  
Occlusal Plane ◽  
Removable Partial Denture ◽  
Cross Sectional ◽  
Partial Denture ◽  
Medical College ◽  
Inferior Border ◽  
Relationship Of ◽  
Mongolian Ethnicity

Background: Determining occlusal plane is challenging and difficult aspect during complete removable partial denture prosthesis rehabilitation. Numerous soft tissue landmarks like commissures of the mouth by Gillis in 1933, parotid papilla by P. F. Foley and G. H. Latta in 1985, height of the retromolar pad, the lateral borders of the tongue etc. had been proposed to assist clinician for determining the occlusal plane. No universally accepted reliable anatomical measurement has been proposed to assist in determining of occlusion plane for different ethnic groups in context to Nepal. Material & Methods: This cross-sectional study was conducted in the 60 dentate subjects visiting Nobel Medical College, Nepal, from February 2019 to July 2019. A customized occlusal plane relator was used to evaluate relative parallelism between occlusal plane and alatragal line with respect to different borders of tragus. All the data were collected and statistically analyzed. Results: Maxillary occlusal plane was found to be parallel with the line drawn from ala of the nose to the middle of tragus in 66.66%, followed by inferior border of tragus in 18.33% with the least parallelism in superior border of tragus 15%. Relationship of maxillary occlusal plane with alatragal line was not statistically significant among Aryans and Mongolian ethnicity Conclusion: This study showed line joining ala of the nose and middle border of tragus is parallel to maxillary occlusal plane. And no difference was found for Aryan & Mongolian ethnicity in relation to alatragal line and maxillary occlusal plane.

scholarly journals Blood group and its relationship with bleeding time and clotting time- an observational study among the 1st MBBS students of Gauhati medical college, Guwahati

2017 ◽  
Vol 5 (9) ◽  
pp. 4147 ◽  
Author(s):  
Reeta Baishya ◽  
Raj Sarkar ◽  
Barasha Barman
Keyword(s):  
Blood Group ◽  
Bleeding Time ◽  
Cross Sectional Study ◽  
Clotting Time ◽  
Chi Square ◽  
Cross Sectional ◽  
Medical College ◽  
The Difference ◽  
Relationship Of ◽  
The Relationship

 Background: Blood group and its relation to bleeding time (BT) and clotting time (CT) is important in conditions like epistaxis, thrombosis, and surgery. Earlier studies depicted O group having prolonged BT and CT. The aim of this study was to assess the relationship of BT and CT with ABO groups.Methods: This cross-sectional study was conducted in the department of physiology, Gauhati medical college. The study included 154 students. Blood grouping was determined with the standard antiserum; BT and CT were estimated by the duke method and slide method, respectively. Blood group and its relation to BT, CT were analyzed by Chi-square analysis.Results: Blood group O (42.2%) was predominant in both genders followed by B (31.8%), A (21%), AB (4.5%). CT was found to be more than 6 minutes in group O (31.57%) followed by group AB (26.31%), A and B (21.05%). The difference was statistically significant (p = 0.02). BT was found to be more than 4 minutes in group O (82.35%) followed by A (11.76%), B (5.88%), and AB (0%). That was statistically significant (p = 0.01). CT was more than 6 minutes in 57.89% in females as compared to 42.10% in males, variation was statistically significant (p = 0.01). BT was more than 4 minutes in 58.82% females as compared to 41.17% in males. The variation was statistically significant (p = 0.03).Conclusions: In present study blood group O was more common followed by B, A, and AB. CT and BT were prolonged in O group. BT and CT were more in females than males.

scholarly journals Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography

2019 ◽  
Vol 84 (9) ◽  
pp. 943-950
Author(s):  
Nikola Gligorijevic ◽  
Milos Sunderic ◽  
Aleksandra Vilotic ◽  
Marko Baralic ◽  
Olgica Nedic
Keyword(s):  
End Stage Renal Disease ◽  
Covalent Binding ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Partial Denaturation ◽  
Stage Renal Disease ◽  
End Stage ◽  
And Function ◽  
Structure Of Proteins

A simple and reliable method for the determination of the concentration and function of alpha-2-macroglobulin (?2M) by zymography was developed. The method is based on the covalent binding of ?2M and trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the electrophoretic gel. The results showed that ?2M binds trypsin in a concentration-dependent manner exhibiting a linear relation. The sensitivity of the method is 125 nM and the intra-assay coefficient of variation 4.2 %. Freezing of ?2M induces its partial denaturation, which could be seen as the reduction in the amount of functional molecule and its reactivity with trypsin. The reported method enables measurement of ?2M taking into consideration both its quantity and function, stressing the importance of the determination of the amount of physiologically active molecules and not just their presence in the sample. The method was further confirmed using ?2M from patients with end-stage renal disease who are known to be under increased oxidative stress and inflammation, which are expected to modify the structure of proteins.

scholarly journals Hubungan Status Gizi dengan Perkembangan Anak Umur 24-60 Bulan di Kelurahan Bener Kota Yogyakarta

2019 ◽  
Vol 4 (3) ◽  
pp. 122
Author(s):  
Reni Merta Kusuma
Keyword(s):  
Nutritional Status ◽  
Growth And Development ◽  
Pearson Correlation ◽  
Cross Sectional Study ◽  
The Body ◽  
Cross Sectional ◽  
Nutritional Status Of Children ◽  
And Function ◽  
Physical Changes ◽  
Relationship Of

Background: Toddler growth and development monitoring is required. The growth is increasing the size of the cells, cell count, and intracellular tissue. Physical changes associated with growth and body structure. The progression is the increase of the function of the structure and function of the body. Growth and development of coupled, so it is important to monitor. The importance of monitoring the growing swell toddlers, researchers want to find out the correlation of these two variables.Objective: To find out the relationship between nutritional status and development of infants in Bener Village, Yogyakarta CityMethods: The draft cross-sectional study with data capture at any given moment. The population of a number of 84 children aged 24-60 months. The sampling techniques used total sampling so that the sample amounted to 84. The data obtained will be analyzed by testing the continent Koefisiensi test with Pearson Correlation Lamda.Results: The research results showed that the nutritional status of children aged 24-60 months in Kelurahan Bener 81% 3.6% normal and stated stated skinny as hell. Development of a toddler aged 24-60 months in Kelurahan Bener 89.3% 2.4% expressed and revealed irregularities. Bivariat analysis results declared between nutritional status with the development of a toddler aged 24-60 months value p of 0.493. Position korelasinya is very weak with the value of the correlation of Pearson of 0.076.Conclusion: Conclusion there is no relationship of nutritional status with the development of a toddler aged 24-60 months and correlation of both positions is very weak.

Perceived social support and burden of care of male and female caregivers of patient with schizophrenia

2017 ◽  
Vol 8 (1) ◽  
pp. 10
Author(s):  
Narendra Kumar Singh ◽  
Nishant Goyal
Keyword(s):  
Social Support ◽  
Perceived Social Support ◽  
Family Members ◽  
Well Being ◽  
Cross Sectional Study ◽  
Burden Of Care ◽  
Cross Sectional ◽  
Male And Female ◽  
The Social ◽  
The Difference

Background: Schizophrenia is associated with a high familial, social and economic burden. Schizophrenia is also associated with a high level of disability which may create impediments on the social and economic areas of the patients as well as on their respective family networks. Families with schizophrenia may encounter problems such as impairment of health and well being of other family members, restriction of social activities of the family members and shrinking of support from the social network. Aims: The present study examined the difference in perceived social support and burden of care between the male and female caregivers of patients with schizophrenia. Methods: This was a cross-sectional study examining the difference in perceived social support and burden of care between the male and female caregivers of patients with schizophrenia. The sample consisted of 60 (30 male and 30 female) caregivers of the patients with the diagnosis of schizophrenia as per ICD-10-DCR. Results and Conclusion: This study revealed that male caregivers perceived more social support and less burden of care as compared to female caregivers. Key words: Gender, social support, burden

Hepatitis A Seroprevalence Among Patients with Chronic Hepatitis B Infection: A Cross-Sectional Study in Iran During 2016-2017

2020 ◽  
Vol 20 (5) ◽  
pp. 748-751
Author(s):  
Seyed Ali Dehghan Manshadi ◽  
Neda Alijani ◽  
Mohammadreza Salehi ◽  
Omid Dadras ◽  
SeyedAhmad SeyedAlinaghi ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Hepatitis A ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Cross Sectional ◽  
Reasonable Approach ◽  
The Difference ◽  
Prevention Method

Introduction: The aim of this study was to determine the prevalence of exposure to hepatitis A by means of serologic markers in chronic hepatitis B patients, with the secondary aim of finding the best prevention method for hepatitis A infection in susceptible groups of our setting. Methods: During the period between 2016 and 2017, we recruited 403 hepatitis B patients aged more than 14 years and regularly attending the infectious diseases clinic at a referral university hospital, Tehran, Iran. A blood sample was collected from all the patients and tested for hepatitis A IgG. The data was analyzed by SPSS v.19. Results: Although none of the patients had previously received hepatitis A vaccine, the results for serologic level of hepatitis A IgG, demonstrated positive results in 379 (94%) cases. The mean age of patients with negative and positive IgG was 29.17 and 42.46 years, respectively; the difference was statistically significant (P≤0.001). The majority of seronegative patients were young adults aged < 25 years and 25 to 35 years (P <0.001). Conclusion: Seroprevalence of hepatitis A in chronic HBV patients in Iran is high. As HBV infected patients younger than 35 years could be seronagative for HAV infection, evaluation of these patients for HAV infection and vaccination of seronegative patients would be a reasonable approach.

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