scholarly journals 1290. Ceftazidime-Avibactam Resistance Report in a Third Level Hospital in Mexico City

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S734-S734
Author(s):  
Aaron Molina ◽  
Abigail Salinas-Hernandez ◽  
Alejandro Olmedo-Reneaum
Keyword(s):  
Inhibition Zone ◽  
International Studies ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Gram Negative Organisms ◽  
Tract Infections ◽  
Abdominal Infections ◽  
Resistance Rates

Abstract Background The surge of resistant Gram-negative organisms has been worrying infectious disease physicians and physicians in general because of the lack of a large number of antibiotics to which these organisms remain susceptible. Ceftazidime-Avibactam (CAZ-AVI) is a drug approved by the FDA to treat complicated urinary tract infections (cUTI), complicated intra-abdominal infections (cIAIs) in combination with metronidazole, and recently for the treatment of nosocomial pneumonia. Worldwide resistance rates of Enterobacteriaceae to CAZ-AVI have been reported below 2.6%, and 4-8% for Pseudomonas aeruginosa. The FDA, CLSI, and EUCAST assigned the clinical breakpoints of susceptibility: MIC < /=8 mg/liter susceptible, and >/=8mg/liter, resistant. In Mexico, CAZ-AVI was approved in 2018, and its cost is very high compared to other antimicrobials, so its use is limited in very specific cases. The resistance rates to this antibiotic in the Mexican population remain largely unknown. Methods We tested 106 specimens for susceptibility to ceftazidime-avibactam using the disk Kirby-Bauer method. The inhibition zone diameter was determined in all cases and we considered the organism susceptible when the inhibition zone diameter was >=21 mm, and resistant with an inhibition zone diameter < = 20 mm. Results We found 5 specimens (4.71%) resistant to ceftazidime-avibactam, corresponding to E. coli (3) and P. aeruginosa (2). Two of these were also resistant to colistin, and 4 to meropenem. All carbapenem-resistant isolates harbored Metallo-beta-lactamases genes, for E. coli was NDM gen, and for P. aeruginosa the VIM gene(GeneXpert® Cepheid). Conclusion The ceftazidime-avibactam resistance among Gram-negative bacteria in our study is similar to the one reported in other international studies. We need more studies in our population to know the nationwide resistance to this antibiotic. Disclosures All Authors: No reported disclosures

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals URINARY TRACT INFECTIONS

2016 ◽  
Vol 23 (01) ◽  
pp. 010-014
Author(s):  
Muhammad Usman Anjum ◽  
Muhammad Safdar Khan ◽  
Abdul Razzaq Shahid ◽  
Syed Humayun Shah
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Geographical Area ◽  
Gram Negative ◽  
E Coli ◽  
Amoxicillin Clavulanic Acid ◽  
Gram Negative Organisms ◽  
Tract Infections ◽  
Micro Organisms

Background: Urinary tract infections (UTIs) constitute important bacterialdisease which contributes to significant morbidity world-wide. Empirical treatment in patientssuffering from UTI depends upon the local knowledge of common microorganisms responsiblefor UTI in that geographical area as well as their antimicrobial susceptibility patterns.Objectives: To determine the frequency and antimicrobial susceptibility of uropathogenswhich are responsible for urinary tract infections. Study Design: Experimental study. Setting:Department of Pathology, Frontier Medical & Dental College and Mohi Ud Din Islamic MedicalCollege. Period: January 2015 to June 2015. Material & methods: Total of 113 patients wereincluded in the study. Urine samples were cultured on MacConkey’s agar and Cysteine LactoseElectrolyte Deficient (CLED) agar. Micro-organisms were identified using standard tests andantimicrobial susceptibility was checked using modified Kirby Bauer method following Clinicaland Laboratory Standards Institute (CLSI) guidelines. Results: The average age of patientswas 32.19±16.47 years. Gram negative organisms accounted for majority of cases, about 89(78.76%) cases. Escherichia coli was the most common micro-organism which was found in50 (44.24%) cases followed by Staphylococcus aureus in 24 (21.24%), Enterobacter spp. in19 (16.81%), Klebsiella spp. in 11 (9.73%) and Proteus spp. in 9 (7.96%) cases. E. coli wassensitive to imipenem and ciprofloxacin and was resistant to amoxicillin/clavulanic acid andgentamicin. Conclusion: Gram negative organisms are largely responsible for UTIs and E. colibeing the most common etiological agent. E. coli is sensitive to commonly prescribed drugs forUTI like ciprofloxacin.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

scholarly journals EFFECTIVENESS OF UTILIZING MIANA LEAVES (COLEUS SCUTTELARIOIDES (L) BENTH) ON THE GROWTH OF ESCHERICHIA BACTERIA (E. COLI) AS A CAUSE OF DIARRHEA AT DRINKING WATER REFILL CENTER

2021 ◽  
Vol 56 (5) ◽  
pp. 494-503
Author(s):  
Nani Yuniar ◽  
Yusuf Sabilu ◽  
Juminten ◽  
Suryani ◽  
Ruslan Madjid ◽  
...  
Keyword(s):  
Drinking Water ◽  
Experimental Research ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Effective Concentration ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Significant Difference

This research aims to determine the inhibition and effectiveness of using miana leaf extract against the growth of E. coli bacteria that causes diarrhea. This study uses experimental research with samples of miana leaves extracted and E. coli bacteria and then uses the Kruskal-Wallis test with Mann-Whitney analysis to test the hypothesis. The results showed differences in the inhibition between miana leaves extract concentrations of 15%, 30%, 50%, and 90% of the growth of E. coli bacteria with Asymp values. Sig = 0.017. The most effective concentration of miana leaf extract in influencing E. coli bacteria was an extract concentration of 90% with an inhibition zone diameter of 31.27 mm. There was a significant difference in the inhibition between miana leaf extract (Coleus scutellarioides (L) Benth) on the growth of E. coli bacteria at 12, 15 & 18 hours, 20 hours, and 24 hours, with Asymp values. Sig = 0.000. Results an increase in the inhibition zone, and the highest inhibition occurred during the 24 hours, namely at a very strong 90% extract concentration. This finding proves that the compounds contained in miana leaf extract can inhibit the growth of E. coli bacteria. This finding has implications for refill drinking water managers to utilize miana leaf extract to avoid E. coli bacteria as the main cause of diarrhea.

scholarly journals DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2015 ◽  
Vol 17 (1) ◽  
pp. 149
Author(s):  
Fahri Bian ◽  
Febby E.F. Kandou ◽  
Marhaenus J. Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta  terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan  29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

scholarly journals Potensi Alga Merah dan Alga Hijau untuk Menghambat Pertumbuhan Bakteri Eschericia coli

2020 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
KETUT SRIE MARHAENI JULYASIH ◽  
NI PUTU RISTIATI ◽  
IDA BAGUS PUTU ARNYANA
Keyword(s):  
Inhibition Zone ◽  
Seaweed Extract ◽  
Inhibition Zone Diameter ◽  
Bacteria Growth ◽  
E Coli ◽  
Zone Diameter ◽  
Bacterial Agent ◽  
Synthetic Chemicals ◽  
Test Parameters ◽  
Escherichia Coli Bacteria

Potential of Red Algae and Green Algae to Inhibit Escherichia coli Bacteria Growth. The use of synthetic chemicals as a control for the growth of E. coli bacteria that causes of diarrhea children can cause adverse effects on health, so it needs natural control ingredients that do not cause impacts on human health. One natural controller to inhibit bacterial growth is by utilizing natural sources, including seaweed. The purpose of this study was to examine the potential of seaweed as an anti-bacterial agent of E. coli. The research used seaweed extract of Eucheuma spinosum, Euchuema cottonii, Gracilaria spp., and Caulerpa spp. The test parameters observed were the inhibition zone (mm) of each seaweed extract treatment using a ruler and measured the inhibition zone distance. The results showed that E.cottonii seaweed extract  had the highest inhibitory ability with an inhibition zone diameter of 11.1 mm, including the moderately sensitive category, then Caulerpa spp. with a inhibition zone diameter of 10.0 mm, Gracilaria spp has the ability to inhibit E.coli bacteria with a inhibition zone diameter of 6.9 mm, including the insensitive category, and the lowest in seaweed E. spinosum with a inhibition zone diameter of 4.2 mm. including the insensitive category.

scholarly journals Aktivitas Antibakteri Ekstrak Metanol Tumbuhan Paku diantum capillus-veneris dan Asplenium nidus Terhadap Bakteri Gram Negatif Escherichia coli Dengan Metode Difusi Agar

Jurnal MIPA ◽  
2018 ◽  
Vol 7 (1) ◽  
pp. 25
Author(s):  
Febby E.F Kandou ◽  
Dingse Pandiangan
Keyword(s):  
Escherichia Coli ◽  
Base Material ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Adiantum Capillus Veneris ◽  
Asplenium Nidus

Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak metanol tumbuhan paku Adiantum capillus-veneris dan Asplenium nidus terhadap pertumbuhan bakteri Gram negatif Escherichia coli. Pengujian aktivitas ekstrak menggunakan Metode Difusi Agar (tes Kirby Bauer), yaitu metode difusi dengan kertas cakram untuk menentukan aktivitas antimikroba. Hasil penelitian diperoleh ekstrak metanol Adiantum capillus-veneris memiliki aktivitas dalam menghambat pertumbuhan Escherichia coli pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 0,00 mm dan 6,80 mm. Ekstrak Asplenium nidus menghambat pertumbuhan Escherichia coli pada konsentrasi 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 3,60 mm, 7,20 mm dan 12,50 mm.  Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak Adiantum capillus-veneris pada konsentrasi 90% menghambat Escherichia coli pada kategori sedang. Ekstrak Asplenium nidus tergolong dalam kategori sedang dan kuat dalam menghambat bakteri Gram negatif Escherichia coli sehingga memiliki potensi untuk dikembangkan sebagai bahan dasar antibakteri.The aim of this research to test the methanol extract activity of Adiantum capillus-veneris and Asplenium nidus on the growth of Gram negative bacteria Escherichia coli. Testing activity of the extracts to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. The results are Adiantum capillus-veneris extract inhibit the growth of E. coliat concentrations of extract 30%, 60% and 90% with inhibition zone diameter respectively 0.00 mm, 0.00. mm and 6.80 mm; and Asplenium nidus extract inhibit the growth of E. coli with inhibition zone diameter respectively 3.60 mm, 7.20 mm and 12.50 mm. Based on the results obtained that the extract of Adiantum capillus-veneris classified in medium category and Asplenium nidus extract in medium and strong category activity toward Gram negative bacteria Escherichia coli, so that has potential as a base material antibacterial.

scholarly journals UJI DAYA HAMBAT EKSTRAK METANOL Selaginella delicatula DAN Diplazium dilatatum TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

2015 ◽  
Vol 15 (1) ◽  
pp. 52 ◽  
Author(s):  
Stery B. Oroh ◽  
Febby E.F. Kandou ◽  
Johanis Pelealu ◽  
Dingse Pandiangan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methanol Extract ◽  
Base Material ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRAK Penelitian ini bertujuan untuk menguji daya hambat ekstrak metanol Selaginella delicatula dan Diplazium dilatatum terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian daya hambat ekstrak terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak S. delicatula menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 6.70 mm dan 7.80 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 8.40 mm dan 13.00 mm. Ekstrak D. dilatatum menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 6.70 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 8.00 mm. Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak S. delicatula dan D. dilatatum tergolong dalam kategori kuat dan sedang serta memiliki potensi sebagai bahan dasar antibakteri, karena kedua ekstrak menunjukkan daya hambatnya. Kata kunci : tumbuhan paku, Selaginella delicatula, Diplazium dilatatum, Antibakteri, Staphylococcus aureus, Escherichia coli INHIBITION TEST OF METHANOL EXTRACT OF Selaginella delicatula AND Diplazium dilatatum AGAINST BACTERIA Staphylococcus aureus AND Escherichiacoli ABSTRACT This study aimed to test the inhibition of methanol extract of Selaginella delicatula and Diplazium dilatatum on the growth of Staphylococcus aureus and Escherichia coli. Testing inhibition of the extracts to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. S. delicatula extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 6.70 mm and 7.80 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 8.40 mm and 13.00 mm. D. dilatatum extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 0.00 mm and 6.70 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 0.00 mm and 8.00 mm. Based on the results obtained, it can be concluded that the extract of S. delicatula and D. dilatatum classified in the category of medium and strong and has potential as a base material for both extracts showed antibacterial inhibitory power. Keywords: ferns, Selaginella delicatula, Diplazium dilatatum, Antibacterial, Staphylococcus aureus, Escherichia coli.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK BIJI KAPULAGA (Amomum compactum Sol. Ex Maton)

2016 ◽  
Vol 17 (2) ◽  
pp. 119-129
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Muhamad Zaenudin
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Amomum Compactum

Testing of antibacterial activity against ethyl acetate extract local cardamom seeds (Amomum compactum Sol. Ex Maton) has been performed. Extraction was carried out using the soxhlet method with methanol solvent and liquid-liquid partitioned with n-hexane, ethyl acetate and n-butanol solvent, antibacterial activity test was performed using the disc diffusion method, fractionation using column chromatography and characterization of active fractions using chromatography GCMS, UV-vis and FTIR spectroscopy. The test results showed that the antibacterial activity of ethyl acetate extract had the highest antibacterial activity against S. aureus and E. coli with inhibition zone diameter of respectively 15.15 ± 1.34 and 13.50 ± 0.70 mm at a concentration of 3200 mg/mL. Results of fractionation of the ethyl acetate fraction using column chromatography with a mobile phase of ethyl acetate: n-hexane (3: 2) yielded three fractions, namely F1 (14.6 mg), F2 (8.1 mg) and F3 (4.6 mg). Fraction 2 had the highest antibacterial activity against S. aureus with inhibition zone diameter of 12.34 ± 0.07 mm at a concentration of 800 ug/mL. The results of the characterization of the fraction 2 obtained using GCMS analysis of three antibacterial compounds suspected of 2.9-dihydroxy-1,8-cineol; 2,4-dihydroxy-1,8-cineol and 2,2-methylene bis [6- (1,1-dimethylethyl) -4-ethyl] phenol. The results of the F2 fraction characterization using UV-Vis spectroscopy showed the presence of group C = C conjugated chromophore at λmax 223 nm and are based on analysis using FTIR there -OH alcohol functional group (3372 cm-1), aliphatic -CH (2926 and 2854 cm-1) , C = C (1695 cm-1), aliphatic CH2 (1402 cm-1), CH3 aliphatic (1384 cm-1), and C-O (1203; 1126; 1091 and 1043 cm-1). Keywords: Antibacterial, S. aureus, E. coli, Amomum compactum Sol. Ex Maton, disk diffusion

scholarly journals Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)

2018 ◽  
Vol 26 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Hansani N.K.S. Pathirana ◽  
Sudu H.M.P. Wimalasena ◽  
Benthotage C.J. De Silva ◽  
Sabrina Hossain ◽  
Gang-Joon Heo
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Paralichthys Olivaceus ◽  
Inhibition Zone ◽  
Olive Flounder ◽  
Gram Negative ◽  
Inhibition Zone Diameter ◽  
Citrus Aurantifolia ◽  
Zone Diameter

Abstract The antibacterial activity of lime (Citrus aurantifolia) essential oil (LEO) and limonene was tested against seven Gram-negative and nine Gram-positive fish pathogenic bacteria isolated from cultured olive flounder, Paralichthys olivaceus (Temminck & Schlegel) in Korea. Limonene was >99% concentrated and LEO consisted of eleven chemical compounds including 56.22% of limonene. Disk diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests were done. LEO and limonene inhibited the growth of both Gram-negative and Gram-positive bacteria. LEO and limonene (MBC/MIC= 2-8) were both bactericidal and bacteriostatic for the strains tested. In every fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100% (V/V) concentrations of LEO and limonene. The antibiogram pattern indicated that all the bacterial strains, excluding three strains of S. iniae (S186, S530, and S131), showed resistance to one or more antibiotics. The percentage of the relative inhibition zone diameter (RIZD %) exhibited high values at higher concentrations of all the agents. Since antibacterial activities of LEO and limonene were considerably effective against fish pathogenic bacteria, they could be used as alternatives to treat bacterial infections in aquaculture.

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