scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK BIJI KAPULAGA (Amomum compactum Sol. Ex Maton)

2016 ◽  
Vol 17 (2) ◽  
pp. 119-129
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Muhamad Zaenudin
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Amomum Compactum

Testing of antibacterial activity against ethyl acetate extract local cardamom seeds (Amomum compactum Sol. Ex Maton) has been performed. Extraction was carried out using the soxhlet method with methanol solvent and liquid-liquid partitioned with n-hexane, ethyl acetate and n-butanol solvent, antibacterial activity test was performed using the disc diffusion method, fractionation using column chromatography and characterization of active fractions using chromatography GCMS, UV-vis and FTIR spectroscopy. The test results showed that the antibacterial activity of ethyl acetate extract had the highest antibacterial activity against S. aureus and E. coli with inhibition zone diameter of respectively 15.15 ± 1.34 and 13.50 ± 0.70 mm at a concentration of 3200 mg/mL. Results of fractionation of the ethyl acetate fraction using column chromatography with a mobile phase of ethyl acetate: n-hexane (3: 2) yielded three fractions, namely F1 (14.6 mg), F2 (8.1 mg) and F3 (4.6 mg). Fraction 2 had the highest antibacterial activity against S. aureus with inhibition zone diameter of 12.34 ± 0.07 mm at a concentration of 800 ug/mL. The results of the characterization of the fraction 2 obtained using GCMS analysis of three antibacterial compounds suspected of 2.9-dihydroxy-1,8-cineol; 2,4-dihydroxy-1,8-cineol and 2,2-methylene bis [6- (1,1-dimethylethyl) -4-ethyl] phenol. The results of the F2 fraction characterization using UV-Vis spectroscopy showed the presence of group C = C conjugated chromophore at λmax 223 nm and are based on analysis using FTIR there -OH alcohol functional group (3372 cm-1), aliphatic -CH (2926 and 2854 cm-1) , C = C (1695 cm-1), aliphatic CH2 (1402 cm-1), CH3 aliphatic (1384 cm-1), and C-O (1203; 1126; 1091 and 1043 cm-1). Keywords: Antibacterial, S. aureus, E. coli, Amomum compactum Sol. Ex Maton, disk diffusion

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI

2019 ◽  
Vol 6 (1) ◽  
pp. 59
Author(s):  
Priska Nancy Claudia Bali ◽  
Ahmad Raif ◽  
Setia Budi Tarigan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibition Zone Diameter ◽  
Negative Controls ◽  
Pandanus Amaryllifolius ◽  
Positive Controls

Pandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

scholarly journals Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

2020 ◽  
Vol 11 (1) ◽  
pp. 52-60
Author(s):  
Sanusi Ibrahim ◽  
Suryati Suryati ◽  
Enda Desriansyah Aziz
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Cytotoxicity Activity ◽  
E Coli ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Ethyl Acetate Extracts

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

scholarly journals Karakterisasi Senyawa Hasil Isolasi dari Ekstrak Etil Asetat Daun Namnam (Cynometra Cauliflora L.) yang Memiliki Aktivitas Antibakteri

2016 ◽  
Vol 2 (1) ◽  
pp. 55-62
Author(s):  
Tiah Maharani ◽  
Dede Sukandar ◽  
Sandra Hermanto
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Biological Activities ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Main Peak ◽  
Disc Diffusion ◽  
Key Word

Namnam is known as a plant family of fabacea that contains phenolic compounds with diverse biological activities including antibacterial. The aim of this study was to isolate and characterize the antibacterial activity of compounds isolated from ethyl acetate extract of the leaves namanam (Cynometra cauliflora L.). Extraction was done by maceration, fractionation by column chromatography, antibacterial essay by disc diffusion method, and characterization of compounds with UV-Vis, FTIR spectroscopy and LCMS. Ethyl acetate extracts have antibacterial activity with inhibition zone respectively 12.25 and 6.00 mm to Escherecia coli and Stapilococcus aureus at a concentration of 100,000 ppm. The results of column chromatography fractions 23-30 yielded three isolates with Rf 0.20 cm; 0.33 cm and 0.87 cm. Characteristics of antibacterial active compounds in isolates 2 (Rf 0.33 cm) is based on the analysis results are UV-Vis absorption at λmaks 206.93 nm, 268.40 nm, 328.58 nm, 383.98 nm and 386.98 nm , FTIR (KBr) showed -OH group 3415.68 cm-1, CH 2958.10 cm-1, C = C aromatic 1651.18 cm-1, C-OH cyclic 1019.88 cm-1 and CH aromatic 694.56 cm-1 and LCMS produce three main peak at a retention time of 4.82; 6.87 and 7.64 which is thought to be the compound 2-isopropyl-5-metilsikloheksil 2-hidroksipropanoat, Cuelure, and 2-[(2-Hydroxycyclohexyl) oxy] cyclohexanecarboxylate.   Key word: antibacterial, disc diffusion, Namnam (Cynometracauliflora L.). DOI: http://dx.doi.org/10.15408/jkv.v2i1.3084

scholarly journals Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

2021 ◽  
Vol 869 (1) ◽  
pp. 012033
Author(s):  
D R Utami ◽  
I Irwan ◽  
S Agustina ◽  
S Karina ◽  
S Afriani
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Hexane Extract ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Hexane Extracts ◽  
Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

2020 ◽  
Vol 8 (3) ◽  
pp. 177
Author(s):  
Achmad Syarifudin Noor ◽  
Bawon Triatmoko ◽  
Nuri Nuri
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction ◽  
Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Hendra D.A. Dharsono ◽  
Mieke H. Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Steroid Glycoside ◽  
Oral Bacteria ◽  
Epiphytic Plant

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

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