Dissecting MicroRNA–mRNA Regulatory Networks Underlying Sulfur Assimilation and Cadmium Accumulation in Poplar Leaves

2020 ◽  
Vol 61 (9) ◽  
pp. 1614-1630
Author(s):  
Wenguang Shi ◽  
Wenzhe Liu ◽  
Chaofeng Ma ◽  
Yuhong Zhang ◽  
Shen Ding ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Populus Deltoides ◽  
Cadmium Accumulation ◽  
Exposure Condition ◽  
Sulfur Assimilation ◽  
Cd Accumulation ◽  
Regulation Mechanisms ◽  
Sulfur Level ◽  
Cd Exposure ◽  
Poplar Leaves

Abstract The process of cadmium (Cd) accumulation and detoxification under different sulfur levels remains largely unknown in woody plants. To investigate the physiological and transcriptomic regulation mechanisms of poplars in response to different sulfate (S) supply levels and Cd exposure, we exposed Populus deltoides saplings to one of the low, moderate and high S levels together with either 0 or 50 µM Cd. Cd accumulation was decreased in low S-treated poplar leaves, and it tended to be increased in high S-supplied leaves under the Cd exposure condition. Sulfur nutrition was deficient in low S-supplied poplars, and it was improved in high S-treated leaves. Cd exposure resulted in lower sulfur level in the leaves supplied with moderate S, it exacerbated a Cd-induced sulfur decrease in low S-treated leaves and it caused a higher sulfur concentration in high S-supplied leaves. In line with the physiological changes, a number of mRNAs and microRNAs (miRNAs) involved in Cd accumulation and sulfur assimilation were identified and the miRNA–mRNA networks were dissected. In the networks, miR395 and miR399 members were identified as hub miRNAs and their targets were ATP sulfurylase 3 (ATPS3) and phosphate 2 (PHO2), respectively. These results suggest that Cd accumulation and sulfur assimilation are constrained by low and enhanced by high S supply, and Cd toxicity is aggravated by low and relieved by high S in poplar leaves, and that miRNA–mRNA regulatory networks play pivotal roles in sulfur-mediated Cd accumulation and detoxification in Cd-exposed poplars.

The Possible Mechanisms of Cadmium Accumulation in Tagetes erecta L. under Macronutrient Deficiency

2012 ◽  
Vol 178-181 ◽  
pp. 1060-1064
Author(s):  
Qian Feng ◽  
Pei Dong Tai ◽  
Sha Sha Fu ◽  
Pei Jun Li ◽  
Yin Qiu Zhang
Keyword(s):  
Organic Acids ◽  
Cadmium Accumulation ◽  
Nutrient Supply ◽  
Culture Conditions ◽  
Tagetes Erecta ◽  
Nutrient Deficiencies ◽  
Cd Accumulation ◽  
Tagetes Erecta L ◽  
Cd Transport ◽  
Cd Exposure

An experiment was conducted under hydroponic culture conditions to determine effects of nutrient deficiencies on cadmium (Cd) accumulation of Tagetes erecta L. and its possible mechanisms. Seedlings of similar size were pre-cultured in either N- or P- deprived nutrient solution for 7 days before exposure to 0.1 mg Cd L-1. The control plants had normal nutrient supply before and during Cd exposure. The plants were harveste after 7 days exposure to Cd. In N-deprived plants, Cd levels in roots, stems and roots were significantly reduced, which was consistent with a decrease of phytochelatins (PCs) in roots. Cd accumulation was the lowest in N-deficient plants, corresponding to substantial decreases in organic acids. In P-deprived plants, the obvious increase of Cd content in the root and stem and decrease of Cd content in the leaf accorded with increases of citric acid in the root and malic acid in the stem, supporting the suggestion that organic acids sequester soluble Cd and reduce Cd transport into the leaf.

scholarly journals Potential Risk of Consuming Vegetables Planted in Soil with Lead and Cadmium and the Influence on Vegetable Antioxidant Activity

2021 ◽  
Vol 11 (9) ◽  
pp. 3761
Author(s):  
Wen-Lii Huang ◽  
Wei-Hsiang Chang ◽  
Shu-Fen Cheng ◽  
Huai-Yuan Li ◽  
Hsiu-Ling Chen
Keyword(s):  
Sweet Potato ◽  
Contaminated Soils ◽  
Vegetable Consumption ◽  
Accumulation Rate ◽  
Leafy Vegetables ◽  
Water Spinach ◽  
Potato Leaf ◽  
Cd Accumulation ◽  
Lead And Cadmium ◽  
Cd Exposure

Once in soil and water, metals can enter the food chain, and the consumption of contaminated crops can pose a serious risk to human health. This study used pot experiments to evaluate the accumulation of metal elements and their influence on levels of antioxidants in vegetables. The current study clearly demonstrates that metals accumulated in the five vegetables that were planted in the contaminated soils, especially so for water spinach. Cd accumulation of all of the vegetables planted in the contaminated soils was greater Cu. The low accumulation rate that was seen in sweet potato leaf, potato, and tomato indicated their suitability for planting in suspected contaminated soil, such as at farms nearby metal industries, in replacement of high accumulators, such as leafy vegetables. The non-carcinogenic HI of Cd exposure from water spinach and sweet potato were >1, whereas those for Cu were <1. This study suggests that residents may experience health risks due to vegetable consumption, and that children are vulnerable to the adverse effects of heavy metal ingestion.

scholarly journals Influence of elevated content of cadmium and arsenic in diet containing feeding yeast on organisms of rats

2009 ◽  
Vol 54 (No. 1) ◽  
pp. 1-9 ◽  
Author(s):  
J. Száková ◽  
V. Zídek ◽  
D. Miholová
Keyword(s):  
Sodium Arsenite ◽  
Cadmium Accumulation ◽  
Yeast Cells ◽  
Male Rats ◽  
Arsenic Content ◽  
Cadmium Content ◽  
Cd Accumulation ◽  
Experimental Animals ◽  
Natural Level ◽  
Liver And Kidney

The influence of elevated cadmium content in diet on the content of this element in liver, kidney and testes of 68 male rats was studied in dependence on the chemical form of applied cadmium (as inorganic salt – CdCl<sub>2</sub> and organically bound in yeast cells); the influence of elevated arsenic content (as NaAsO<sub>2</sub>) in diet on its content in the same organs was also investigated. The interactions between arsenic and cadmium in the above-mentioned organs were studied. The addition of cadmium to the diet of rats significantly (<I>P</I> < 0.05) increased cadmium content in several organs. The addition of yeast containing the natural level of Cd increased the content of cadmium in liver and kidney of experimental animals significantly (<I>P</I> < 0.05). A significantly (<I>P</I> < 0.05) increased cadmium accumulation in organs was observed after the addition of Cd as CdCl<sub>2</sub>, compared with the addition of Cd as organically bound Cd in yeast cells. At the same time, the addition of yeasts containing the natural level of Cd decreased the Cd accumulation applied as CdCl2 in the examined organs. The addition of sodium arsenite to the diet of rats led to a significantly (<I>P</I> < 0.05) increased arsenic content in all the analyzed organs. The addition of yeasts to the diet increased arsenic content in liver and at the same time suppressed its content in kidneys of experimental animals. The interaction between arsenic and cadmium applied simultaneously was evident. The addition of As to the diet decreased the accumulation of Cd in kidney and increased its accumulation in testes. The addition of Cd to the diet increased arsenic content in liver and kidney and decreased its content in testes.

scholarly journals Selenium Enhances Cadmium Accumulation Capability in Two Mustard Family Species—Brassica napus and B. juncea

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 904 ◽  
Author(s):  
Zhong-Wei Zhang ◽  
Yi-Ying Dong ◽  
Ling-Yang Feng ◽  
Zong-Lin Deng ◽  
Qiang Xu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Juncea ◽  
Brassica Species ◽  
Dry Weight ◽  
Cadmium Accumulation ◽  
Cd Accumulation ◽  
Cd Toxicity ◽  
Valence States ◽  
Early Seedling ◽  
High Level

Oilseed rape (Brassica napus) is a Cadmium (Cd) hyperaccumulator. However, high-level Cd at the early seedling stage seriously arrests the growth of rape, which limits its applications. Brassica juncea had higher Cd accumulation capacity, but its biomass was lower, also limiting its applications. Previous studies have confirmed that Selenium (Se) can alleviate Cd toxicity. However, the regulatory mechanism of Se in different valence states of Cd accumulation was unclear. In this study, we investigated the ameliorating effects of three Se valence states, Na2SeO4 [Se(VI)], Na2SeO3 [Se(IV)] and Se-Met [Se(II)], to Cd toxicity by physiological and biochemical approaches in hydroponically-cultured Brassica juncea and Brassica napus seedlings. Although Se treatments slightly inhibited seedling Cd concentration, it tripled or quadrupled the Cd accumulation level per plant, because dry weight increased about four times more with Se and Cd application than with Cd treatment alone. Among the different valence states of Se, Se(II) had the most marked effect on reducing Cd toxicity as evidenced by decreased growth inhibition and Cd content. The application of Se(II) was effective in reducing Cd-induced reactive oxygen species accumulation, and promoted the antioxidant enzyme activity and photosynthesis of both Brassica species. In addition, Se(II) treatment increased the concentrations of Cd in the cell wall and soluble fractions, but the Cd concentration in the organelle part was reduced.

scholarly journals Reducing Cadmium Accumulation in Plants: Structure–Function Relations and Tissue-Specific Operation of Transporters in the Spotlight

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 223 ◽  
Author(s):  
Xin Huang ◽  
Songpo Duan ◽  
Qi Wu ◽  
Min Yu ◽  
Sergey Shabala
Keyword(s):  
Structure Function ◽  
Current Knowledge ◽  
Cadmium Accumulation ◽  
Major Focus ◽  
Phloem Tissue ◽  
Cd Uptake ◽  
Major Health ◽  
Cd Accumulation ◽  
Tissue Specific

Cadmium (Cd) is present in many soils and, when entering the food chain, represents a major health threat to humans. Reducing Cd accumulation in plants is complicated by the fact that most known Cd transporters also operate in the transport of essential nutrients such as Zn, Fe, Mn, or Cu. This work summarizes the current knowledge of mechanisms mediating Cd uptake, radial transport, and translocation within the plant. It is concluded that real progress in the field may be only achieved if the transport of Cd and the above beneficial micronutrients is uncoupled, and we discuss the possible ways of achieving this goal. Accordingly, we suggest that the major focus of research in the field should be on the structure–function relations of various transporter isoforms and the functional assessment of their tissue-specific operation. Of specific importance are two tissues. The first one is a xylem parenchyma in plant roots; a major “controller” of Cd loading into the xylem and its transport to the shoot. The second one is a phloem tissue that operates in the last step of a metal transport. Another promising and currently underexplored avenue is to understand the role of non-selective cation channels in Cd uptake and reveal mechanisms of their regulation.

Studies on the Mechanism of Cadmium Accumulation in Tagetes erecta L

2010 ◽  
Vol 113-116 ◽  
pp. 211-217
Author(s):  
Yin Qiu Zhang ◽  
Pei Dong Tai ◽  
Pei Jun Li ◽  
Qing Zhao ◽  
Dian Bo Dong
Keyword(s):  
Protein Synthesis ◽  
Plant Growth ◽  
Growth Inhibitor ◽  
Cadmium Accumulation ◽  
Protein Synthesis Inhibitor ◽  
Tagetes Erecta ◽  
Synthesis Inhibitor ◽  
Cd Accumulation ◽  
Tagetes Erecta L ◽  
Cell Division Inhibitor

Hydroponic experiments were carried out to study the effects of cell division inhibitor (Maleic hydrazide MH) and protein synthesis inhibitor（Cycloheximide CHI）on Cd accumulation in Tagetes erecta L and their possible mechanisms. Seedlings were exposed to 4 mmol L-1 MH or 3 µmol L-1 CHI in 0.1 mg L-1 Cd-containing solution for 3 days. After treatment, the Cd content in leaves was significantly (P<0.05) reduced under MH or CHI treatment, which was consistent with the decreased soluble protein contents in leaves, suggesting that protein synthesis plays a role in Cd accumulation in leaves of Tagetes erecta L. Both of the plant growth inhibitors led to increased Cd contents in roots compared with the control, corresponding with increased GSH, Cys or γEC content under MH or CHI treatment, indicating that those S-containing compounds may be related to the increased Cd content in roots under the plant growth inhibitor treatments. On the other hand, the two inhibitors increased the citric acid content in all the tissues of Tagetes erecta L except for that in leaves under CHI treatment. All the results suggest that protein synthesis may be responsible for Cd accumulation in leaves of Tagetes erecta L, while the S-containing compounds and organic acids are associated with Cd absorption in roots of Tagetes erecta L.

Effect of Environmental Factors on Cd Accumulation of Tagetes erecta L.

2011 ◽  
Vol 71-78 ◽  
pp. 149-155
Author(s):  
Sha Sha Fu ◽  
Xin Fa Xu ◽  
Rong Fang Li ◽  
Pei Dong Tai
Keyword(s):  
Transpiration Rate ◽  
Cadmium Accumulation ◽  
Hydroponic Culture ◽  
Effect Of Temperature ◽  
Nutrient Level ◽  
Tagetes Erecta ◽  
Nutrient Element ◽  
Sulfur Deficiency ◽  
Culture Experiment ◽  
Cd Accumulation

A hydroponic culture experiment was conducted to study the effect of temperature and nutrient level on Cd accumulation inTagetes erectaL.. In the first experiment, shoot Cd accumulation and transpiration rate at different temperature was determined. Cd accumulation was lower with higher transpiration rate at 35°C than that of 25°C or 15°C with lower transpiration rate. In the other experiment, cadmium accumulation of plants varies significantly under the condition of different nutrient supplication. Contents of Cd in roots were not high, but the contents of Cd in shoots were the highest when plants were cultivated in 0.05-fold Hoagland’s solution. Roots Cd accumulation decreased as the concentration of single nutrient element (N, P, S) decrease or absence, and shoots Cd accumulation decrease in the condition of sulfur deficiency and sulfur absence. It was shown that transpiration is not the primary factor affecting Cd accumulation inTagetes erectaL, but growth rate of plants has certain correlation with Cd accumulation; contents of Cd in roots are most affected by the supplication of nutrient elements, yet shoots accumulation is relatively lagging behind; different nutrient element has some different effect on Cd accumulation, and the effects of nitrogen and sulfur on the shoots Cd accumulation are the greatest.

Cadmium accumulation and apoplastic and symplastic transport in Boehmeria nivea (L.) Gaudich on cadmium-contaminated soil with the addition of EDTA or NTA

RSC Advances ◽  
2015 ◽  
Vol 5 (59) ◽  
pp. 47584-47591 ◽  
Author(s):  
Yicheng Yin ◽  
Yaqin Wang ◽  
Yunguo Liu ◽  
Guangming Zeng ◽  
Xinjiang Hu ◽  
...  
Keyword(s):  
Plant Species ◽  
Contaminated Soil ◽  
Cadmium Accumulation ◽  
Nitrilotriacetic Acid ◽  
Ethylene Diamine ◽  
Cd Accumulation ◽  
Symplastic Transport ◽  
Boehmeria Nivea ◽  
Tolerant Plant ◽  
Cadmium Contaminated Soil

A Cd-tolerant plant species named Boehmeria nivea (L.) Gaudich (ramie) was applied to study its Cd accumulation and translocation mechanisms with the addition of ethylene diamine tetracetic acid (EDTA) or nitrilotriacetic acid (NTA).

scholarly journals Overexpression of OsLCT2, a Low-Affinity Cation Transporter Gene, Reduces Cadmium Accumulation in Shoots and Grains of Rice

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Li Tang ◽  
Jiayu Dong ◽  
Longtao Tan ◽  
Zhongying Ji ◽  
Yaokui Li ◽  
...  
Keyword(s):  
Genetic Resource ◽  
Xylem Sap ◽  
Cadmium Accumulation ◽  
Cd Accumulation ◽  
Rice Grains ◽  
Hydroponic Experiment ◽  
Zn Uptake ◽  
Transporter Family ◽  
Molecular Regulatory Mechanisms ◽  
Cd Translocation

AbstractCadmium (Cd)-contaminated rice is a serious issue affecting food safety. Understanding the molecular regulatory mechanisms of Cd accumulation in rice grains is crucial to minimizing Cd concentrations in grains. We identified a member of the low-affinity cation transporter family, OsLCT2 in rice. It was a membrane protein. OsLCT2 was expressed in all tissues of the elongation and maturation zones in roots, with the strongest expression in pericycle and stele cells adjacent to the xylem. When grown in Cd-contaminated paddy soils, rice plants overexpressing OsLCT2 significantly reduced Cd concentrations in the straw and grains. Hydroponic experiment demonstrated its overexpression decreased the rate of Cd translocation from roots to shoots, and reduced Cd concentrations in xylem sap and in shoots of rice. Moreover, its overexpression increased Zn concentrations in roots by up-regulating the expression of OsZIP9, a gene responsible for Zn uptake. Overexpression of OsLCT2 reduces Cd accumulation in rice shoots and grains by limiting the amounts of Cd loaded into the xylem and restricting Cd translocation from roots to shoots of rice. Thus, OsLCT2 is a promising genetic resource to be engineered to reduce Cd accumulation in rice grains.

scholarly journals Cadmium: An Emerging Role in Adipose Tissue Dysfunction

2021 ◽  
Author(s):  
Sarra Mohammed Attia ◽  
Kavitha Varadharajan ◽  
Muralitharan Shanmugakonar ◽  
Sandra Concepcion Das ◽  
Hamda A. Al-Naemi
Keyword(s):  
Adipose Tissue ◽  
Inflammatory Markers ◽  
Toxic Heavy Metal ◽  
Cd Accumulation ◽  
Toxic Impact ◽  
Anthropogenic Inputs ◽  
Industrial Sectors ◽  
Adipose Tissue Macrophages ◽  
Anti Inflammatory ◽  
Cd Exposure

AbstractCadmium (Cd) is a toxic heavy metal that is widespread in the environment due to the substantial anthropogenic inputs from the agriculture and industrial sectors. The toxic impact of Cd adversely affects human health and is linked with endocrine disruption, carcinogenicity, diabetes-related diseases, and metabolic disorder. One of the main characterizations of Cd is bioaccumulation where its half-life reaches 40 years with an unknown biological role. Several organs were found to be targets for Cd accumulation such as the liver, kidneys, and adipose tissue. Adipose tissue (AT) is a dynamic organ that plays a significant role in the body’s homeostasis through the maintenance of energy storage. Another vital function for AT is the secretion of adipokines which provides a metabolic cross-talk with the whole body’s organs. Cd is found to adversely impact the function of AT. This includes the disruption of adipogenesis, lipogenesis, and lipolysis. As a consequence, dysfunctional AT has disruptive patterns of adipokines secretions. The main adipokines produced from AT are leptin and adiponectin. Both were found to be significantly declined under the Cd exposure. Additionally, adipose tissue macrophages can produce either anti-inflammatory markers or pro-inflammatory markers depending on the local AT condition. Cadmium exposure was reported to upregulate pro-inflammatory markers and downregulate anti-inflammatory markers. However, the exact mechanisms of Cd’s adverse role on AT structure, function, and secretion patterns of adipokines are not totally clarified. Therefore, in this review, we present the current findings related to Cd detrimental effects on adipose tissues.

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