scholarly journals Pavement markings: identification of relevant covariates and controllable factors of retroreflectivity performance as a road safety measure

2021 ◽  
Author(s):  
Linda Lee Ho ◽  
Júlio Silvio de Souza Bueno Filho ◽  
Walquiria Yumiko Fujii ◽  
Cláudia A S Machado ◽  
Liedi Legi Bariani Bernucci ◽  
...  
Keyword(s):  
Road Safety ◽  
Fixed Effects ◽  
Block Design ◽  
Mixed Linear Model ◽  
Traffic Volume ◽  
Rural Roads ◽  
Pavement Markings ◽  
Randomized Block Design ◽  
Controllable Factors ◽  
High Traffic Volume

Abstract In this paper we present relevant contributions and important features related to the study of the retroreflectivity performance of pavement markings. The contribution of this paper is threefold. First, we propose an artificial scheme to allow some randomization of the treatments owing to several restrictions imposed on the choice of the experimental units. It is an experiment involving one fixed factor (three types of materials) in a randomized block design executed on a high-traffic-volume highway. Under this condition, the traffic volume works as a stress factor and the degradation of the retroreflectivity of pavement markings is faster than the degradation on rural roads or streets. This is related to the second contribution: the possibility of a reduction of experimental time. The current experiment spent 20 weeks to collect the data. And finally a mixed linear model considering three random effects and several fixed effects is fitted and the most relevant effects pointed out. This study can help highway managers to improve road safety by scheduling the maintenance of pavement marks at the appropriate time, choosing adequate material for the pavement markings and applying the proposed artificial scheme in future studies.

scholarly journals 251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  
Keyword(s):  
Great Plains ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Omega 3 ◽  
Finishing Diets ◽  
Randomized Block Design ◽  
Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

528 Late-Breaking: Late Gestation Metabolizable Energy Intake Increases Colostrum Yield and Alters Colostrum Composition in Beef Cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 150-150
Author(s):  
Koryn S Hare ◽  
Emily Croft ◽  
Katharine M Wood ◽  
Michael A Steele
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Compositional Analysis ◽  
Repeated Measurements ◽  
Metabolizable Energy ◽  
Late Gestation ◽  
Beef Cow ◽  
Randomized Block Design ◽  
Milking Machine ◽  
Beta Hydroxybutyrate

Abstract The objective was to determine how late gestation metabolizable energy (ME) intake impacts beef cow colostrogenesis. Angus-Simmental primiparous (n = 47) and multiparous (n = 109) cattle were blocked by expected calving date and randomly assigned to receive diets that supplied 80% (LME; n = 53), 100% (CME; n = 52), or 120% (HME; n = 51) of the ME requirement. Treatments were balanced by initial BW and fed for 53 d prior to calving. Cow body weight was recorded d -53, -39, -25, -10, and -3 relative to calving and retrospectively conceptus-corrected. Calves were weighed and measured prior to suckling. Colostrum was hand-stripped from all cows for compositional analysis and a subset (n = 16/treatment) was fully milked using a milking machine. Data were analyzed (PROC GLIMMIX) as a randomized block design, including the fixed effects of treatment, parity, and treatment×parity and the random effects of block and cow(block). Day and its interactions were included for repeated measurements. Treatment did not affect (P ≥ 0.73) cow BW or conceptus-corrected BW. Calf chest circumference was lesser (treatment×parity: P = 0.023) for primiparous vs. multiparous calves if their dams consumed LME or CME, but not if their dams consumed HME. Feeding HME rather than LME increased (P = 0.006) colostrum yield. The LME diet increased (P = 0.004) colostrum IgG concentration over HME; however, HME colostrum yielded more (P = 0.005) total IgG. Crude protein and urea-N were elevated (P < 0.001) in LME colostrum, whereas HME colostrum contained more (P = 0.026) lactose. Low ME colostrum fat was greater (P = 0.013) than CME and beta-hydroxybutyrate was increased (P < 0.001) in LME vs. CME and HME colostrum. These data provide insight regarding nutrient partitioning during late gestation and demonstrate that beef cow colostrogenesis is responsive to prepartum ME intake.

scholarly journals PSIV-10 Preference evaluation of plant functional types grasses in calves

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 225-226
Author(s):  
María A Vulliez ◽  
María Bruni ◽  
Ana I Trujillo ◽  
Martín Durante ◽  
Lucrecia Lezana ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Mixed Model ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Functional Types ◽  
Randomized Block Design ◽  
Functional Types Of Plants ◽  
Group B ◽  
Cut Leaves

Abstract The functional types of plants (PFT) ecological approach could be used to link the preferences of ruminants. In order to investigate if ruminant preferences were associated with this approach, three PFT groups were evaluated. A group “B”, represented by Paspalum urvillei (PU-B), “C” by Nassella neesiana (NN-C) and Sporobolus indicus (SI-C) and “D” by Andropogon lateralis (AL-D). In a randomized block design with repeated measures over time, two independent cafeteria trials (autumn-winter, A-W, and spring-summer, S-S) were carried out in calves (n = 8 for A-W; n = 4 for S-S; BW=240–270 kg) arranged in four pens. In three sessions of ten minutes each, calves received 120–160 gDM of fresh cut leaves from the different species. Dry matter intake (DMI), nitrogen intake (NI) and neutral detergent fiber intake (NDFI) were measured. Data were analyzed with a mixed model that included specie and session as fixed effects and blocks as a random. The statistical analyses were performed with SAS (University Edition), data is expressed as lsmeans and was considered to differ when Tukey test presented P < 0.05. No significant differences were observed between sessions. The DMI and NI were higher in NN-C and lower in AL-D (P < 0.01) during A-W. However, in S-S, higher DMI and NI were observed in AL-D and SI-C while NN-C presented the lower DMI and NI (P < 0.01). The FDNI did not show differences in A-W meanwhile in S-S was positively associated with DMI (Table1). Interestingly, when the season changed, it was observed a change in PFT preference, in A-W calves preferred NN-C whereas in S-S preferred AL-D. However, attributes associated with preferences were maintained in both stations, for instance, higher DMI was associated with higher NI. Additional research is required on the interactions between PFT approach and the preference of animals for different species.

scholarly journals Phytate-phosphorus and phytase contents on the relative weight of organs, intestinal morphometry and performance of broilers

2016 ◽  
Vol 46 (10) ◽  
pp. 1858-1864
Author(s):  
Aiane Aparecida da Silva Catalan ◽  
Everton Luis Krabbe ◽  
Valdir Silveira de Avila ◽  
Letícia dos Santos Lopes ◽  
Ana Paula Nunes ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Relative Weight ◽  
Average Weight ◽  
Feed Conversion ◽  
Phytate Phosphorus ◽  
Randomized Block Design ◽  
Random Block ◽  
And Performance ◽  
Intestinal Morphometry

ABSTRACT: In order to evaluate the efficiency of phytase in diets with low and high phytate phosphorus (PP) 0content, as a consequence of wheat bran inclusion, on the relative weight of organs, intestinal morphometry and performance, three hundred and eighty-four male Cobb500 broilers were housed in metabolic cages. Animals were assigned into four treatments in a 2x2 factorial scheme in a randomized block design with eight replicates of 12 birds each. From 11 days of age birds received experimental diets, which consisted of: Diet low in PP; Diet low in PP with phytase (500FTU kg-1); Diet with a high PP and Diet with a high PP with phytase (500FTU kg-1). At 22 and 32 days of age two birds were slaughtered in order to collect gizzard, heart, liver, cecum, cloacal bursa, and at 32 days, a portion of the duodenum, jejunum and ileum was collected for morphometric evaluation. From 22 to 32 days of age average feed intake, average weight gain, average body weight and feed conversion ratio were also evaluated. Data were subjected to analysis of variance, fixed effects of diet and phytase and interaction between factors as well as the random block effects were tested. There was no significant interaction for the variables studied, concluding that phytase in diets with low or high phytate phosphorus content did not change the relative weight of organs, intestinal morphometrics and performance; only isolated effects were observed.

scholarly journals PSVI-19 Evaluating the addition of an alpha-amylase enzyme to two bulk-flake density weights of corn fed to beef finishing steers

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 219-220
Author(s):  
Caleb Lockard ◽  
Cathy Lockard ◽  
Wyatt Smith ◽  
Brian Campbell ◽  
Jenny Jennings
Keyword(s):  
Fixed Effects ◽  
Mixed Model ◽  
Block Design ◽  
Carcass Characteristics ◽  
Feedlot Cattle ◽  
Alpha Amylase ◽  
Beef Steers ◽  
Finishing Cattle ◽  
Subsequent Performance ◽  
Randomized Block Design

Abstract An alpha-amylase enzyme has the potential to increase starch digestibility, thus improving feedlot cattle performance and efficiency. The objective of this experiment was to evaluate the addition of an alpha-amylase enzyme on two steam-flaked bulk densities and subsequent performance of finishing beef steers. Forty-eight crossbred steers (325 ± 19 kg), were used in a complete randomized block design with a 2 × 2 factorial treatment design. Factors were corn processing and enzyme addition. Dietary treatments were steam-flaked corn-based diets with two bulk-flake density weights of corn (0.32 kg/L vs. 0.39 kg/L). Experimental treatments were 1) 0.32 kg/L with no enzyme (32NE), 2) 0.39 kg/L with no enzyme (39NE), 3) 0.32 kg/L with enzyme (32E), and 4) 0.39 kg/L with enzyme (39E) on a DM basis. The enzyme was applied at 0.50 kg/T of steam-flaked corn (As-fed basis). Data were analyzed using a mixed model with fixed effects of flake weight, enzyme, and flake weight × enzyme interaction. Throughout the feeding period, starch availability for 0.32 kg/L and 0.39 kg/L corn averaged 60.7% and 42.2%, respectively. Steers receiving the alpha-amylase enzyme had no differences in BW (P ≥ 0.62), ADG (P ≥ 0.55), and DMI (P ≥ 0.56) compared to NE steers. Furthermore, carcass characteristics did not differ (P ≥ 0.26) between E and NE steers. Feeding an alpha-amylase enzyme to finishing cattle had no detrimental effects on performance or carcass characteristics. Overall, a more highly processed corn was observed to decrease dry matter intake and increase the potential for digestive upsets. This may limit the use of the alpha-amylase enzyme to a lesser processed corn.

scholarly journals 393 Beef cow antepartum glucose-insulin kinetics are altered by overfeeding metabolizable protein during late gestation and influenced by test-day relative to calving

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 157-157
Author(s):  
Koryn S Hare ◽  
Greg B Penner ◽  
Katie M Wood
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Area Under The Curve ◽  
Late Gestation ◽  
Ratio Test ◽  
Intravenous Glucose ◽  
Metabolizable Protein ◽  
Insulin Kinetics ◽  
Randomized Block Design

Abstract The objective of this study was to determine whether antepartum glucose-insulin kinetics were affected when primiparous Hereford-cross heifers were fed diets containing 100 (CON; n = 10) or 133% (HMP; n = 11) of their metabolizable protein requirements from d -55.3 ± 3.7 until parturition. Intravenous glucose tolerance tests (IVGTT) were performed on d -5.4 and -7.1 ± 1.3 (CON and HMP, respectively; P = 0.34) by collecting baseline (-10 min) plasma, infusing 1.36 g glucose/kg BW0.75 via a jugular catheter, and subsequently sampling plasma for analysis of glucose and insulin. Data were analyzed with PROC GLIMMIX as a randomized block design with the fixed effects of diet, time (minute), and diet × time, considering cow (block) as a random effect. Pearson correlations were performed for IVGTT variables and test-day relative to parturition. Differences and trends were declared at P < 0.05 and 0.05 ≤ P < 0.10, respectively. Basal glucose was 13.5% greater (P = 0.048) and basal insulin tended to be 28.8% greater (P = 0.068) for HMP than CON heifers. Plasma glucose (-10 to 120 min) was greater (P = 0.047) for HMP than CON, whereas insulin was not (P = 0.54). Glucose and insulin were respectively elevated (time: P < 0.001) at 0 and 5 and 5 to 20 min and both equilibrated to baseline by 90- and 120-min. Diet × time tended to affect (P = 0.061) the glucose:insulin ratio. Test-day was correlated with time-to-max insulin (ρ = 0.60, P = 0.006), insulin clearance rate (ρ = 0.48, P = 0.027), and glucose area-under-the-curve (ρ = -0.47, P = 0.031). Time relative to calving can influence antepartum glucose-insulin kinetics. Oversupplying metabolizable protein increased baseline glucose and insulin and glucose throughout the IVGTT. Antepartum beef cattle may have altered energy partitioning that is responsive to protein consumption.

Innovation of Natural Preservation of Mackerel (Rastrelliger kanagurta) Using Coconut Coconut Ashes with Drying Time Variations on Hedonics

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Rika Agustina ◽  
Rita Sunartaty ◽  
Teuku Makmur
Keyword(s):  
Research Design ◽  
Block Design ◽  
Drying Time ◽  
Basic Ingredient ◽  
Randomized Block Design ◽  
Salt Substitute ◽  
Long Time ◽  
Time Variations ◽  
Rastrelliger Kanagurta

Coconut frond ash is one of the wastes from coconut trees which has not been maximally utilized. Coconut frond ash contains MgCl2 and KCl so that it can be used as a salt substitute in the process of preserving fish. In this study coconut frond ash was used as a basic ingredient for making dried mackerel with a long time of drying to storage. The purpose of this study was to determine the effect of drying time on mackerel storage. The research design used was a Randomized Block Design (RCBD) with 2 factors studied. The first factor is the drying time consists of 3 levels, namely P1 = 3 days, P2 = 4 days, P3 = 5 days. The second factor is storage which consists of 3 levels, namely S1 = 30 days, S2 = 60 days, S3 = 90 days. Each treatment was repeated 2 times to obtain 18 experimental units to observed hedonic tests. From the results of the study it can be stated that the treatment has a very significant effect (P≥0.01) on the hedonic test which includes (color, aroma, taste and texture).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

Combining ability and heterosis studies across environments in linseed (Linum usitatissimum L.)

2011 ◽  
Vol 59 (1) ◽  
pp. 87-102 ◽  
Author(s):  
S. Sood ◽  
N. Kalia ◽  
S. Bhateria
Keyword(s):  
Seed Yield ◽  
Linum Usitatissimum ◽  
Combining Ability ◽  
Yield Components ◽  
Block Design ◽  
Linum Usitatissimum L ◽  
Randomized Block Design ◽  
Component Traits ◽  
Parental Lines ◽  
Fibre Yield

Combining ability and heterosis were calculated for fourteen lines of linseed in a line × tester mating design using twelve lines and two diverse testers in two different environments. The hybrids and parental lines were raised in a completely randomized block design with three replications to investigate seed and fibre yield and their component traits. Genetic variation was significant for most of the traits over environments. Combining ability studies revealed that the lines KL-221 and LCK-9826 were good general combiners for seed yield and most of its components, whereas LMH-62 and LC-2323 were good general combiners for yield components only. Moreover, KL-221 was also a good general combiner for fibre yield. Similarly, B-509 and Ariane were good general combiners for fibre yield and most of its components. Among the specific cross combinations, B-509 × Flak-1 was outstanding for seed yield per plant and B-509 × KL-187 and LC-2323 × LCK-9826 for fibre yield per plant, with high SCA effects. In general, the hybrids excelled their respective parents and the standard checks for most of the characters studied. Based on the comparison of mean performance, SCA effects and the extent of heterosis, the hybrids LC-2323 × LCK-9826 and B-509 × KL-221 appeared to be the most promising for both seed and fibre yield. Other promising combinations were LC-2323 × KL-210 and B-509 × Ariane for seed and fibre yield, respectively. The superiority of LC-2323, LCK-9826, KL-221, B-509 and Ariane as good general combiners was further confirmed by the involvement of these parents in the desirable cross combinations.

PENGARUH KADAR PROTEIN DAN LEMAK PAKAN TERHADAP PERTUMBUHAN DAN KOMPOSISI BADAN IKAN KERAPU MACAN, Epinephelus fuscoguttatus

2016 ◽  
Vol 5 (2) ◽  
pp. 277
Author(s):  
Usman Usman ◽  
Neltje Nobertine Palinggi ◽  
Kamaruddin Kamaruddin ◽  
Makmur Makmur ◽  
Rachmansyah Rachmansyah
Keyword(s):  
Lipid Content ◽  
Lipid Level ◽  
Block Design ◽  
High Growth ◽  
Biological Variables ◽  
Randomized Block Design ◽  
Tiger Grouper ◽  
Test Fish ◽  
Net Cages ◽  
Protein Diets

Penelitian ini dilakukan untuk mendapatkan informasi tentang kebutuhan optimum kadar protein dan lemak pakan terhadap pertumbuhan dan komposisi badan ikan kerapu macan ukuran konsumsi. Sembilan pakan uji dibuat dalam bentuk moist pelet dengan tiga dosis protein (46%, 49%, dan 52%) dan tiga dosis lemak (9%, 11%, dan 13%). Ikan uji dipelihara dalam 27 keramba jaring apung ukuran 1 m x 1 m x 2 m selama 140 hari, diberi pakan uji secara satiasi dua kali sehari dan diset dalam rancangan acak kelompok pola faktorial berdasarkan kelompok ukuran bobot awal ikan yaitu (i) 122,0±4,2 g; (ii) 144,0±7,1 g; dan (iii) 172,9±10,5 g. Hasil penelitian menunjukkan bahwa laju pertumbuhan spesifik dan sintasan ikan relatif sama (>0,05) di antara perlakuan. Efisiensi pakan cenderung meningkat dengan meningkatnya kadar protein dan lemak pakan. Tingkat efisiensi pemanfaatan protein cenderung menurun dengan meningkatnya kadar protein pakan, tetapi meningkat dengan meningkatnya kadar lemak pakan. Hasil analisis proksimat badan ikan menunjukkan bahwa kadar bahan kering dan lemak ikan relatif tidak dipengaruhi (P>0,05) oleh peningkatan kadar protein pakan, namun kadar bahan kering dan lemak ikan tersebut sedikit naik dengan meningkatnya kadar lemak pakan. Kadar protein dan abu ikan relatif tidak dipengaruhi oleh perubahan kadar protein dan lemak pakan. Berdasarkan hasil penelitian ini tampak bahwa pakan dengan kadar protein 49% dan lemak sekitar 11% mampu memberikan pertumbuhan dan komposisi badan ikan kerapu macan yang baik.This experiment was conducted to investigate the optimum dietary protein and lipid level for growth and body composition of tiger grouper. Nine dietary experiments were formulated to moist pellet contain three levels of protein (46%, 49%, and 52%) and three levels of lipid (9%, 11%, and 13%). The fish were fed twice daily to satiation for 140 days in twenty seven net cages of 1 m x 1 m x 2 m, were set up factorial randomized block design based on fish size group i.e. (i) 122.0±4.2 g, (ii) 144.0±7.1 g, and (iii) 172.9±10.5 g. The results shown that specific growth rate and survival rate were not significant different (P>0.05) for all treatments. Feed efficiency increased when protein and lipid content increased. Protein efficiency decreased when protein diets increased and increased when lipid diets increased. No interaction occurred between protein and lipid content to all observable biological variables. Dry matter and lipid content of test fish were not affected by protein diets increase but by lipid diet content. Protein and ash of test fish were not affected by either protein or lipid contained in diet. To assure high growth rates and high quality of fish product, it is suggested to feed tiger grouper with diet containing 49% protein and 11% lipid. 

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