scholarly journals Identification of Plant Compounds Involved in the Microbe-Plant Communication During the Coinoculation of Soybean with Bradyrhizobium elkanii and Delftia sp. strain JD2

2018 ◽  
Vol 31 (11) ◽  
pp. 1192-1199 ◽  
Author(s):  
Célica Cagide ◽  
Braulio Riviezzi ◽  
Manuel Minteguiaga ◽  
María A. Morel ◽  
Susana Castro-Sowinski
Keyword(s):  
Plant Growth ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Plant Communication ◽  
Helper Function ◽  
Plant Growth Promoting ◽  
Bradyrhizobium Elkanii ◽  
Soybean Plants ◽  
Nodulation Rate ◽  
Nutritional Benefits

Delftia sp. strain JD2 is a betaproteobacterium characterized as a plant growth–promoting bacterium with a ‘helper’ function, enhancing the performance of rhizobial inoculant strains during the coinoculation of alfalfa and clover. In this work we analyzed i) the effect of the coinoculation with Bradyrhizobium elkanii and Delftia sp. strain JD2 strains on the performance of soybean plants and ii) the production of a few secondary plant metabolites that would explain the positive effect of coinoculation on the growth and development of soybean plants. The results showed a beneficial effect of coinoculation on soybean growth, nodulation rate, and pulse yield, with the concomitant benefit for the agricultural economy. In addition, based on a metabolomics approach, we demonstrated that a different pattern of plant metabolites is being produced at different stages of plant growth. The new information suggests that the coinoculation of soybean changes the primary and secondary metabolism of the plant, including changes in the metabolic status of main and secondary nodules within the plant. The relevance of producing a different pattern of photosynthetic and photoprotective pigments, flavonoids, organic acids, and carbohydrates are discussed. Finally, we propose that JD2 could be used together with bradyrhizobia to manipulate the chemical composition of plant tissues, promoting the nutritional benefits and health of soybean.

scholarly journals Metabolomics Analyses Reveal Metabolites Affected by Plant Growth-Promoting Endophytic Bacteria in Roots of the Halophyte Mesembryanthemum crystallinum

2021 ◽  
Vol 22 (21) ◽  
pp. 11813
Author(s):  
Ryota Kataoka ◽  
Mami Akashi ◽  
Takeshi Taniguchi ◽  
Yoshiyuki Kinose ◽  
Ahmet Emre Yaprak ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Tricarboxylic Acid Cycle ◽  
Mesembryanthemum Crystallinum ◽  
Initial Growth ◽  
Plant Metabolites ◽  
Ice Plant ◽  
Soil Phytoremediation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mesembryanthemum crystallinum L. (common ice plant) is an edible halophyte. However, if ice plants are used to phytoremediate salinity soil, there are problems of slow initial growth, and a long period before active NaCl uptake occurs under higher salinity conditions. Application of endophytic bacteria may improve the problem, but there remain gaps in our understanding of how endophytic bacteria affect the growth and the biochemical and physiological characteristics of ice plants. The aims of this study were to identify growth-promoting endophytic bacteria from the roots of ice plants and to document the metabolomic response of ice plants after application of selected endophytic bacteria. Two plant growth-promoting endophytic bacteria were selected on the basis of their ability to promote ice plant growth. The two strains putatively identified as Microbacterium spp. and Streptomyces spp. significantly promoted ice plant growth, at 2-times and 2.5-times, respectively, compared with the control and also affected the metabolome of ice plants. The strain of Microbacterium spp. resulted in increased contents of metabolites related to the tricarboxylic acid cycle and photosynthesis. The effects of salt stress were alleviated in ice plants inoculated with the endobacterial strains, compared with uninoculated plants. A deeper understanding of the complex interplay among plant metabolites will be useful for developing microbe-assisted soil phytoremediation strategies, using Mesembryanthemum species.

scholarly journals Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 404
Author(s):  
Atlaw Anbelu Sahile ◽  
Muhammad Aaqil Khan ◽  
Muhammad Hamayun ◽  
Muhammad Imran ◽  
Sang-Mo Kang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Culture Medium ◽  
Bacterial Isolate ◽  
Cadmium Stress ◽  
Cd Stress ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants

Cadmium stress significantly decreases agricultural productivity worldwide. Plant growth-promoting rhizobacteria (PGPR) are eco-friendly and inexpensive tool for mitigating heavy metal stress in crops. We isolated rhizospheric bacteria and screened them for various plant growth-promoting (PGP) traits as well as Cd tolerance. Only 6 bacterial isolates out of 55 assessed showed multiple PGP traits in response to different Cd concentrations. The Bacillus cereus ALT1 strain showed high tolerance to increased Cd amounts in the culture medium, while secreting indole-3-acetic acid (IAA) and organic acids into the culture medium. High Cd concentrations (0.7 mM, 1.4 mM, and 2.1 mM) reduced soybean shoot and root length, root/shoot fresh and dry weight, as well as chlorophyll content; however, inoculation with the bacterial isolate ALT1 mitigated Cd stress and enhanced both soybean growth parameters and chlorophyll content. It also decreased abscisic acid (ABA) amounts, enhanced salicylic acid (SA) production, and promoted antioxidant response by increasing total proteins (TP) and superoxide dismutase (SOD), while decreasing glutathione (GSH) content, lipid peroxidation (LPO), peroxidase (POD), superoxide anion (SOA), and polyphenol oxidase (PPO) in soybean plants. In addition, inductively coupled plasma mass spectrometry (ICP-MS) showed that soybean plants treated with the bacterial isolate ALT1 enhanced K uptake and decreased Cd amounts in comparison to control plants. The present study reveals that Cd-tolerant bacterial isolate ALT1 can alleviate Cd toxicity on plants by increasing their growth, thus imposing itself as an eco-friendly bio-fertilizer under Cd stress.

scholarly journals The Potency of UB Forest Chitinolytic Bacteria to Promote Plant Growth and Inhibit Damping off Disease on Soybean

2021 ◽  
Vol 8 (1) ◽  
pp. 25-33
Author(s):  
Achmad Roekhan ◽  
Ayu Ike Dayanti ◽  
Rahmania Oktaviani ◽  
Fibrianti Shinta ◽  
Nabilla Alya Anastasia ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crude Extract ◽  
Inhibition Test ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Chitinolytic Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants

Damping off disease in soybean plants is caused by the fungal Rhizoctonia solani. The damping off disease causes a yield loss of up to 85-100%. The purpose of this study was determining the ability chitinolytic bacteria consortium of UB Forest's in suppressing damping off disease in vitro and in vivo as well as its potential to stimulate the growth of soybean plants. The research stages included isolation of the pathogenic fungus R. solani and the pathogenicity test. Rejuvenation chitinolytic bacterial isolates of UB Forest, test chitinolytic bacteria antagonist of UB Forest against R. solani fungus, test of Plant Growth Promoting (PGP) activities, synergy test of selected chitinolytic bacterial isolates, in vitro test for the inhibition of chitinase crude extract against R. solani, and inhibition test of chitinolytic bacteria consortium against damping off disease. The selected chitinolytic bacteria were code bacteria UB12, UB19, and UB52 with plant growth promoting activities with inhibition percentage of the pathogen R. solani of 73.9%, 67.4%, and 71.7%. The best chitinolytic bacterial isolates were the genus Bacillus sp. and Pseudomonas sp. The inhibition test of chitinase crude extract showed an inhibition percentage of 25-55%.

scholarly journals Stomata dan Trikoma Kultivar Kedelai Anjasmoro selama pemupukan Nanosilika dan Plant Growth Promoting Rhizobacteria

Vegetalika ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 343
Author(s):  
Sri Suryanti ◽  
Arif Umami
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nano Silica ◽  
Randomized Design ◽  
Silica Concentration ◽  
The Status ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Completely Randomized Design ◽  
Soybean Plants

Soybean is a very important crop commodity in Indonesia. Increasing soybean production on dryland as cultivation land need to be carried out. Dryland have issues with either of nutrient and water availability which is not supportable for plant growth. Therefore, the application of nano-silica and plant growth promoting rhizobacteria (PGPR) are expected to increase nutrient absorption, synthesis of plant growth hormone and increase in plant resistance to drought stress. The growth of drought-resistant plants is able to be showed through the status of stomata and trichomes on leaves. So that, this study aimed to determine the stomata and trichome performance of soybean plants of Anjasmoro cultivar using nano-silica and PGPR as fertilizers. The study design used a completely randomized design with 2 factors with three replication. The first factor was nano silica concentration i.e. 0, 100 and 200 ppm. The second factor was PGPR concentration i.e. 0, 5, 10, and 15%. Results showed that the nanosilica and PGPR applications had no significant effect on stomata and trichome status of Anjasmoro cultivar except for stomatal aperture and the ratio of the size of the stomata openings. A positive correlation has been found in the study between stomata openings and soybean yields.

scholarly journals Purpureocillium lilacinum and Metarhizium marquandii as plant growth-promoting fungi

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9005 ◽  
Author(s):  
Noemi Carla Baron ◽  
Andressa de Souza Pollo ◽  
Everlon Cid Rigobelo
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Dry Mass ◽  
In Planta ◽  
Purpureocillium Lilacinum ◽  
Plant Growth Promoting ◽  
Soybean Plants ◽  
High Yields ◽  
Vitro Analysis

Background Especially on commodities crops like soybean, maize, cotton, coffee and others, high yields are reached mainly by the intensive use of pesticides and fertilizers. The biological management of crops is a relatively recent concept, and its application has increased expectations about a more sustainable agriculture. The use of fungi as plant bioinoculants has proven to be a useful alternative in this process, and research is deepening on genera and species with some already known potential. In this context, the present study focused on the analysis of the plant growth promotion potential of Purpureocillium lilacinum, Purpureocillium lavendulum and Metarhizium marquandii aiming its use as bioinoculants in maize, bean and soybean. Methods Purpureocillium spp. and M. marquandii strains were isolated from soil samples. They were screened for their ability to solubilize phosphorus (P) and produce indoleacetic acid (IAA) and the most promising strains were tested at greenhouse in maize, bean and soybean plants. Growth promotion parameters including plant height, dry mass and contents of P and nitrogen (N) in the plants and in the rhizospheric soil were assessed. Results Thirty strains were recovered and characterized as Purpureocillium lilacinum (25), Purpureocillium lavendulum (4) and Metarhizium marquandii (1). From the trial for P solubilization and IAA production, seven strains were selected and inoculated in maize, bean and soybean plants. These strains were able to modify in a different way the evaluated parameters involving plant growth in each crop, and some strains distinctly increased the availability of P and N, for the last, an uncommon occurrence involving these fungi. Moreover, the expected changes identified at the in vitro analysis were not necessarily found in planta. In addition, this study is the first to evaluate the effect of the isolated inoculation of these fungi on the growth promotion of maize, bean and soybean plants.

scholarly journals Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria

2016 ◽  
Vol 81 (11) ◽  
pp. 1239-1249 ◽  
Author(s):  
Biljana Kiprovski ◽  
Djordje Malencic ◽  
Simonida Djuric ◽  
Mira Bursac ◽  
Jelena Cvejic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Plant Growth ◽  
Positive Impact ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phenylpropanoid Pathway ◽  
Soybean Seedlings ◽  
Isoflavone Content ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants

Plant-growth promoting rhizobacteria (PGPR) elicit activation of phenylpropanoid pathway in plants which leads to phenolics production and enhanced antioxidant capacity. The purpose of this work was to assess the antioxidant activity of soybean plants, Glycine max L., inoculated with PGPR (isolates of Azotobacter chroococcum, Streptomyces sp. and mixture of these) during plant development, as well as yield of inoculated soybean plants. PGPR applied in the experiment stimulated flavonoids and isoflavone synthesis, which enhanced non-enzymatic antioxidant ability of soybean plants. Also, PGPRs stimulated accumulation of daidzein and genistin in soybean seedlings (5-fold and 2-fold compared to control values, respectively). The mixture of PGPRs showed positive impact on antioxidant activity (10-20% higher activity) and yield components of soybean which proposed this inoculum as possibly potent bio-fertilizer in soybean production.

Rhizobacteria AK1 remediates the toxic effects of salinity stress via regulation of endogenous phytohormones and gene expression in soybean

2019 ◽  
Vol 476 (16) ◽  
pp. 2393-2409 ◽  
Author(s):  
Muhammad Aaqil Khan ◽  
Sajjad Asaf ◽  
Abdul Latif Khan ◽  
Rahmatullah Jan ◽  
Sang-Mo Kang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Salinity Stress ◽  
Inductively Coupled Plasma ◽  
Antioxidant Activities ◽  
Nacl Stress ◽  
Rhizospheric Bacteria ◽  
Plant Growth Promoting ◽  
Soybean Plants ◽  
Phosphate Solubilizing

Abstract Salinity stress adversely affects the growth and productivity of different crops. In the present study, we isolated the rhizospheric bacteria Arthrobacter woluwensis AK1 from Pohang beach, South Korea and determined its plant growth-promoting potential under NaCl salt stress (0, 100, and 200 mM). AK1 has phosphate-solubilizing activity and produce siderophores, organic acids, and phytohormones such as gibberellic acid (GA) and indole-3-acetic acid (IAA) that significantly alleviate sodium chloride (NaCl) stress and increase all plant growth attributes. Furthermore, inoculation of AK1 significantly decreased endogenous abscisic acid (ABA) content, extensively regulated the antioxidant activities and mitigated NaCl stress. Similarly, inductively coupled plasma mass spectrometry results showed that soybean plants inoculated with AK1 significantly decreased the amount of sodium (Na+) uptake during NaCl stress after 6 and 12 days. Four genes, auxin resistant 1 (GmLAX1), potassium channel AKT2 (GmAKT2), soybean salt tolerance 1 (GmST1), and salt tolerance-associated gene on chromosome 3 (GmSALT3) were up-regulated, while two genes chloride channel gene (GmNHX1) and Na+/H+ antiporter (GmCLC1) were down-regulated in soybean AK1treated plants. In conclusion, AK1 can mitigate salinity stress, increase plant growth and could be utilized as an eco-friendly bio-fertilizer under salinity stress.

scholarly journals Halotolerant Rhizobacterial Strains Mitigate the Adverse Effects of NaCl Stress in Soybean Seedlings

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Muhammad Aaqil Khan ◽  
Sajjad Asaf ◽  
Abdul Latif Khan ◽  
Arjun Adhikari ◽  
Rahmatullah Jan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Nacl Stress ◽  
Ion Concentration ◽  
Oenothera Biennis ◽  
Artemisia Princeps ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants ◽  
Plant Growth Promoting Traits

Background. Salinity is one of the major abiotic constraints that hinder health and quality of crops. Conversely, halotolerant plant growth-promoting rhizospheric (PGPR) bacteria are considered biologically safe for alleviating salinity stress. Results. We isolated halotolerant PGPR strains from the rhizospheric soil of Artemisia princeps, Chenopodium ficifolium, Echinochloa crus-galli, and Oenothera biennis plants; overall, 126 strains were isolated. The plant growth-promoting traits of these isolates were studied by inoculating them with the soil used to grow soybean plants under normal and salt stress (NaCl; 200 mM) conditions. The isolates identified as positive for growth-promoting activities were subjected to molecular identification. Out of 126 isolates, five strains—Arthrobacter woluwensis (AK1), Microbacterium oxydans (AK2), Arthrobacter aurescens (AK3), Bacillus megaterium (AK4), and Bacillus aryabhattai (AK5)—were identified to be highly tolerant to salt stress and demonstrated several plant growth-promoting traits like increased production of indole-3-acetic acid (IAA), gibberellin (GA), and siderophores and increased phosphate solubilization. These strains were inoculated in the soil of soybean plants grown under salt stress (NaCl; 200 mM) and various physiological and morphological parameters of plants were studied. The results showed that the microbial inoculation elevated the antioxidant (SOD and GSH) level and K+ uptake and reduced the Na+ ion concentration. Moreover, inoculation of these microbes significantly lowered the ABA level and increased plant growth attributes and chlorophyll content in soybean plants under 200 mM NaCl stress. The salt-tolerant gene GmST1 was highly expressed with the highest expression of 42.85% in AK1-treated plants, whereas the lowest expression observed was 13.46% in AK5-treated plants. Similarly, expression of the IAA regulating gene GmLAX3 was highly depleted in salt-stressed plants by 38.92%, which was upregulated from 11.26% to 43.13% upon inoculation with the microorganism. Conclusion. Our results showed that the salt stress-resistant microorganism used in these experiments could be a potential biofertilizer to mitigate the detrimental effects of salt stress in plants via regulation of phytohormones and gene expression.

Effect of plant growth promoting rhizobacteria (PGPR) and water stress on phytohormones and polyamines of soybean

2015 ◽  
Vol 49 (5) ◽  
Author(s):  
Hossein Zahedi ◽  
Samira Abbasi
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Control Treatment ◽  
Rhizobium Japonicum ◽  
Severe Drought ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants

The effect of inoculation of three plant growth promoting rhizobacteria (PGPR) that is <italic>Rhizobium japonicum</italic>, <italic>Azotobacter chroococcum</italic> and <italic>Azospirillum brasilense</italic> and mixture of them on phytohormones and polyamines of soybean under different irrigation regimes was investigated. Drought stress induced by irrigation withholding until 40, 80 and 120 mm evaporation from evaporation pan. However seed bacterization of soybean was accompanied with 20 kg ha<sup>−1</sup> nitrogen. In addition, 20 and 100 kg ha<sup>−1</sup> nitrogen were considered as control treatments. The results showed that drought stress significantly decreased cytokinin, gibberellin and auxin accumulation in plant tissues. By contrast, drought stress led to increase in abscisic acid accumulation in soybean plants. Polyamines that are putrescine and spermidine increased due to drought stress and then decreased under severe drought stress. PGPR application had positive effect on growth promoting phytohormones compared to control treatment. However the highest accumulation of cytokinin, gibberellin and auxin was related to 100 kg ha<sup>−1</sup> nitrogen treatment. In case of abscisic acid PGPR application decreased its accumulation. Asignificant decrease as observed on polyamines accumulation when PGPRs were applied on stressed soybean plants.

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