Point-of-Care Testing in an Acute Tertiary Care Hospital in Singapore

Author(s):  
Chin Pin Yeo ◽  
Wai Yoong Ng
2019 ◽  
Vol 6 (3) ◽  
pp. 828
Author(s):  
Harjit S. Dumra ◽  
Kautuk A. Patel ◽  
Gopal Raval ◽  
Mansi Dandnaik ◽  
Amrish Patel

Background: Electrolyte disorders are common in patients in the emergency department and intensive care unit, and have been associated with increased morbidity and mortality. In this respect sodium and potassium are the most important cations, whose improper adjustment may cause severe neuromuscular disorders. This study was designed to compare values obtained by laboratory and point-of-care testing and also to find most frequent electrolyte abnormalities.Methods: Observational Study was done on 51 patients presenting to Tertiary care Hospital emergency department with altered sensorium between 1st January 2016 to 31st May 2017 fulfilling the inclusion criteria and willing for participation by giving written informed consent. Electrolytes were tested in patients with GCS 14 or less by both point of care system and in the laboratory.Results: The distribution of mean sodium and potassium levels did not differ significantly between two techniques (P-value>0.05). The sodium and potassium levels by POC and laboratory techniques are significantly and positively correlated (P-value<0.001). The distribution of mean along with 95% CI of mean of amount of bias in the estimation of Sodium and Potassium levels by POC against Laboratory method is 3.50 [2.79-4.20] mEq/L and 0.83 [0.55-1.11] mEq/L respectively. The most common electrolyte abnormality was hyponatremia.Conclusions: We concluded that it is advisable to do a point-of-care electrolyte in Emergency department and Intensive care unit. By use of point-of-care testing, we can identify electrolytes imbalance early in emergency department. Point-of-care electrolyte levels had a near comparable value with laboratory electrolyte levels.


Author(s):  
Surbhi Gupta ◽  
Anju Shukla ◽  
Poonam Singh ◽  
Areena H. Siddiqui

Background: Nucleic acid amplification test (NAAT) is considered gold standard in the molecular diagnosis of CoV-2 infection but since it is costly, labor intensive and needs technical expertise, rapid chromatographic immunoassay for the qualitative detection of specific antigens to SARS CoV-2 have been devised. Objectives of this study was to compare the results of Antigen test and NAAT for CoV-2 infection carried out during the months of July and August 2020 by single tertiary care hospital in Lucknow, Uttar Pradesh and to determine the utility of rapid antigen test in the SARS CoV-2 diagnosis.Methods: All the patients who came to our hospital seeking admission during July 2020 and August 2020 were included in the study. A total of 1000 patients were included in this study.Results: Out of a total 1000 cases which were included in the study, 769 cases (76.9%) were found to be SARS CoV-2 negative by both antigen and CBNAAT, 100 cases (10.0%) were SARS CoV-2 positive by both antigen and CBNAAT tests. But in 131 cases (13.1%), antigen was not able to pick up the disease. It was also found that the Cycle Threshold (Ct) value for the discordant group was higher (Mean E= 28, Mean N2=33) when compared to the group where antigen was positive.Conclusions: The present study establishes the role of rapid antigen tests in contributing to the quick, point of care diagnosis of SARS CoV-2. These assays are safe, simple, and fast and can be used in local clinics and hospitals. These tests are very important for real-time patient management and infection control decision.


Author(s):  
Shilpi Hora ◽  
Neeraj Raman ◽  
Mukesh Kumar ◽  
Needa Saneef

Introduction: Tuberculosis (TB) remains a leading cause of death among infectious diseases worldwide. The advent of the CBNAAT was a revolution in the diagnosis of tuberculosis, especially with high incidence and resource poor regions. It can be used close to the point of care by operators with minimal technical expertise, enabling diagnosis of TB and rifampicin Resistance (RIF) concurrently using unprocessed clinical specimens, in less than 2h. Aim: To evaluate the diagnostic performances of Ziehl-Neelsen & CBNAAT techniques in detection of Pulmonary Tuberculosis. Materials and Methods: We retrospectively reviewed the 2414 sputum samples of suspected pulmonary TB. Sputum samples were subjected for ZN staining and CBNAAT.RIF resistance was detected by CBNAAT. Results: Out of 2414 samples, 751 sputum samples were positive by smear microscopy. 1127 Samples were confirmed positive by CBNAAT examination. Majority of cases, 43.57% were in 21-40 yrs age group.24.40% were females and 75.59% were males. Sensitivity of CBNAAT was 100% for sputum positive cases and sensitivity was 22.6% for sputum negative cases. Overall RIF resistance was detected in 49 (4.4%) cases in present study. Conclusion: CBNAAT provide sensitive detection of tuberculosis and rifampicin resistance directly from untreated sputum in less than 2 hours with minimal hands-on time. Keywords: Cartridge-based nucleic acid amplification tests (CBNAAT), Rifampicin(RIF)


2021 ◽  
pp. 74-75
Author(s):  
Bhaswati Ghoshal ◽  
Tunisha Bhattacharya ◽  
Basudha Roy ◽  
Anamika Das

Objective-Perinatal asphyxia is a important cause of admission in sick newborn care unit. Intensive training on neonatal resuscitation of point of care doctors and nurses can change the asphyxia related outcome in a tertiary care hospital. Design – Before after study design. Setting-Tertiary care hospital delivery room and neonatal care unit. Intervention-Intensive neonatal resuscitation hands on training of point of care doctors and nurses as per neonatal resuscitation protocol by National Neonatology Forum. Outcome- There is signicant decrease in perinatal asphyxia related admission in sick newborn care unit in after group. Early neonatal mortality also decreased. Conclusion – Increasing the awareness of appropriate and timely neonatal resuscitation at delivery point change the outcome of neonatal care unit of tertiary care hospital.


Author(s):  
C. Mohan Rao ◽  
Bhaskar Thakur ◽  
Saswat Subhankar ◽  
D. P. Dash

Background: Diagnosis of Extra-pulmonary TB (EPTB) is a challenge. Authors wanted to assess the sites of extra-pulmonary involvement during 2013-2017 in a tertiary care hospital cum medical college. Authors also wanted to evaluate the role of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) in diagnosis of EPTB and compare its efficacy with AFB Culture.Methods: Total 470 EPTB cases diagnosed between 2013 and 2017 from 840 TB treatment records maintained in designated microscopy centre. Specific samples from appropriate sites were taken up for smear for AFB, CBNAAT and AFB culture.Results: There was incremental detection and registration in both TB and EPTB cases from 96 and 50 cases in 2013 to 246 and 150 cases in 2017 respectively. Among the total 470 EPTB cases in 2013-2017 (55.9%), lymph node followed by pleura and abdomen were the organs having maximum involvement. Bone involvement was more witnessed in adult male than children (p <0.05). There was male preponderance. CBNAAT results were 100 % sensitive and 87.5% specific. Lymph node samples and pus elsewhere in the body had much better diagnostic yield than serous effusions.Conclusions: Awareness and availability of diagnostic services in tertiary care institutions has lead to increased reporting of EPTB under RNTCP services. CBNAAT can be also be utilized as a point of care testing for lymph node aspirate and pus specimen.


Vacunas ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Y.M. AlGoraini ◽  
N.N. AlDujayn ◽  
M.A. AlRasheed ◽  
Y.E. Bashawri ◽  
S.S. Alsubaie ◽  
...  

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