scholarly journals Oral Collagen for the treatment of Dermal Atrophy: A systematic review of human trials

2020 ◽  
Author(s):  
Rhydian P Howell-Morris
Keyword(s):  
Human Study ◽  
Collagen Content ◽  
Minor Trauma ◽  
Substantial Impact ◽  
Impaired Wound Healing ◽  
Human Trials ◽  
Beneficial Effects ◽  
Dermal Thickness ◽  
Dermal Collagen ◽  
Collagen Density

BACKGROUND Dermal atrophy (DA) or skin "thinning" can cause a substantial impact on quality of life and, due to barrier function damage, further health problems including cutaneous infection, skin tears and lacerations from minor trauma, impaired wound healing and chronic dermal inflammation. Some dietary products are targeted at therapeutic and functional treatments for skin ageing (of which mild DA is a component); however, while dietary collagen is amongst the most popular, particularly in the form of collagen peptides (CPs), in contrast to reviews for both over-the-counter and under prescription topical treatments for DA (e.g. Tretinoin), there is no reviewed literature of human trials testing the efficacy of orally administered collagen treatments applicable to DA; hence this review. OBJECTIVE To review the literature and assess available randomised-controlled trials (RCTs) testing the efficacy of orally administered collagen treatments for any skin properties that relate to the pathophysiology of DA and suggest their potential for medical and general public use in treating DA. EVIDENCE REVIEW METHOD A PubMed search was conducted using "(collagen) AND (supplementation OR treatment) AND (skin OR dermis)", after which titles and abstracts were screened to decide if they matched the inclusion criteria for review. Results were collected up to 1st of August 2019 and no lower limit on the year of publication was set. MAIN RESULTS Five studies with a total of 430 participants were included for review, with participants aged 24-70. Four out of the five studies used female only participants. The five studies used orally administered CPs, with dosage ranging from 570 mg/d to 10 g/d, running from 8 weeks to 6 months and assessed a range of skin properties relevant to DA including dermal thickness, epidermal thickness, dermal density, dermal collagen content, dermal collagen density and dermal elasticity. One of the studies combined CPs with several antioxidant ingredients to form the treatment and the remaining 4 studies used CPs as the only active ingredient. Methods to control for potential confounders were implemented in most studies including limiting exposure to sun, implementing a pre-treatment period of 1 week or more that controlled the use of cosmetics and intake of certain medications, micronutrient supplements and nutraceuticals with those restrictions continuing for the duration of the study. Given the heterogeneity of outcome measures across studies, quantitative analysis of results was not possible. In summary, the study with the antioxidant combined supplement showed a significant improvement in dermal thickness; two of the studies showed improvement in dermal collagen or pro-collagen content; three of the studies showed improvement in dermal elasticity; three studies showed improvement in dermal density or dermal collagen density; and lastly, no human study was found with the stated objective of assessing CPs effect specifically on DA. CONCLUSION Although definitive mechanistic cause-effect conclusions could not be drawn from the existing studies, they are supportive of beneficial effects of oral CP intake for treating characteristics of dermal atrophy. Further elucidation of the exact mode(s) of action that the CP intake has on improving dermal thickness, dermal density, and other skin biomarkers is necessary, with larger studies including more finely divided experimental and dose-response groups. In conclusion, rigorousness of the trials must be improved to establish a cause-effect relationship between the CP intake and the beneficial effects for the skin atrophy, however potential has been demonstrated.

Conventional Nanofat and SVF/ADSC-Concentrated Nanofat: A Comparative Study on Improving Photoaging of Nude Mice Skin

2019 ◽  
Vol 39 (11) ◽  
pp. 1241-1250 ◽  
Author(s):  
Hui Zheng ◽  
Lihong Qiu ◽  
Yingjun Su ◽  
Chenggang Yi
Keyword(s):  
Nude Mice ◽  
Lower Layer ◽  
Elastic Fiber ◽  
Stromal Vascular Fraction ◽  
Collagen Content ◽  
Elastic Fibers ◽  
Saline Injection ◽  
Fiber Morphology ◽  
Dermal Thickness ◽  
Dermal Collagen

AbstractBackgroundNanofats could improve photoaging. Stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) may play pivotal roles. However, SVFs and ADSCs in nanofats processed by conventional methods cannot be enriched. Some researchers have found that after centrifugation, the SVF/ADSC density increases from top to bottom.ObjectivesThe authors hypothesized that centrifugation can be used to obtain SVF/ADSC-concentrated nanofats that are superior to conventional nanofats in improving the photoaging of skin.MethodsAfter a photoaging model was successfully established in nude mice, the back of each mouse was divided into 4 areas and randomly injected with conventional nanofat, centrifuged nanofat (either the middle or lower layer of centrifuged nanofat), or normal saline. Wrinkles, dermis thickness, dermal collagen content, and elastic fiber morphology were measured and compared at weeks 4 and 8.ResultsCompared with the wrinkles in the physiological saline injection areas, the wrinkles in the areas injected with the 3 nanofats (lower and middle layers of centrifuged nanofat and conventional nanofat) were significantly reduced. All 3 nanofat groups showed increased dermal thickness, increased collagen content, and a more regular distribution of elastic fibers compared with the saline injection areas.ConclusionsThe study established the efficacy of nanofats in improving photoaging by reducing wrinkles and increasing the thickness of dermal collagen, making nanofats a promising novel treatment for photoaging. The SVF/ADSC-concentrated nanofats exhibited the most improvement.

scholarly journals High frequency ultrasound assessment of systemic sclerosis skin involvement: intra-observer repeatability and relationship with clinician assessment and dermal collagen content

2020 ◽  
pp. jrheum.200234
Author(s):  
Victoria A. Flower ◽  
Shaney L. Barratt ◽  
Darren J. Hart ◽  
Amanda B. Mackenzie ◽  
Jacqueline A. Shipley ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
High Frequency ◽  
Convergent Validity ◽  
Objective Assessment ◽  
Collagen Content ◽  
Skin Thickness ◽  
High Frequency Ultrasound ◽  
Dermal Collagen ◽  
The Relationship ◽  
Frequency Ultrasound

Objective The modified Rodnan skin score (mRSS) remains the preferred method for skin assessment in systemic sclerosis (SSc). There are concerns regarding high inter-observer variability of mRSS and negative clinical trials utilising mRSS as the primary endpoint. High frequency ultrasound (HFUS) allows objective assessment of cutaneous fibrosis in SSc. We investigated the relationship between HFUS with both mRSS and dermal collagen. Methods Skin thickness (ST), echogenicity and novel Shear wave elastography (SWE) were assessed in 53 SSc patients and 15 healthy controls (HC) at the finger, hand, forearm and abdomen. The relationship between HFUS parameters with mRSS (n=53) and dermal collagen (10 SSc patients and 10 HC) was investigated. Intra-observer repeatability of HFUS was calculated using intra-class correlation coefficients (ICCs). Results HFUS assessment of ST (hand/forearm) and SWE (finger/hand) correlated with local mRSS at some sites. Subclinical abnormalities in ST, echogenicity and SWE were present in clinically uninvolved SSc skin. Additionally, changes in echogenicity and SWE were sometimes apparent despite objectively normal ST on HFUS. ST, SWE and local mRSS correlated strongly with collagen quantification (rho 0.697, 0.709, 0.649 respectively). Intra-observer repeatability was high for all HFUS parameters (ICCs for ST 0.946-0.978, echogenicity 0.648- 0.865 and SWE 0.953-0.973). Conclusion Our data demonstrates excellent reproducibility and reassuring convergent validity with dermal collagen content. Detection of subclinical abnormalities is an additional benefit of HFUS. The observed correlations with collagen quantification support further investigation of HFUS as an alternative to mRSS in clinical trial settings.

scholarly journals The Impact of Consumption of Dairy Products on Phospholipid Metabolism: A Systematic Review

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1261-1261
Author(s):  
Emad Yuzbashian ◽  
Catherine B Chan
Keyword(s):  
Dairy Products ◽  
Animal Studies ◽  
Meta Analysis ◽  
Phospholipid Metabolism ◽  
Human Studies ◽  
Resistance Risk ◽  
Human Trials ◽  
Beneficial Effects ◽  
Funding Sources ◽  
The Impact

Abstract Objectives Metabolomics approach indicates that circulating phospholipid (PL) and some PL species are associated with a lower insulin resistance risk. Evidence suggests that dairy products' health beneficial effects may pertain to their regulatory influence on PL metabolism. Therefore, we aimed to systematically review the existing literature of animal and human trials to unravel the impact of dairy products on the concentration of PL and its metabolism. Methods Three online databases, including PubMed, Scopus, and Web of Science, were searched to find relevant studies published in peer-reviewed journals between January 2000 and July 2020. Included studies were interventional trials (animal and human) that investigated the effect of dairy or its subtypes on the circulating or liver content of PL and its species. The risk of bias (RoB) in trials in humans and animals was assessed using the revised Cochrane's and SYRCLE's RoB assessment, respectively. Since there was marked methodological heterogeneity, a meta-analysis did not perform. Results In this review, 2427 articles were identified and screened after removing duplicate articles. Following evaluation of the titles and abstracts and then full-text assessment, 17 studies were identified that met the inclusion criteria. Studies were classified according to their type, resulting in nine human trials and eight animal studies. For human studies, the RoB assessment indicated that more than 55% of studies had high RoB. None animal studies receive low RoB because of the lack of methodological information. Findings from human studies revealed that plasma/serum concentration of PL did not change after intervention with dairy products. PL concentration remained stable even after a high dosage of milk supplemented with dairy-derived PL; however, certain PC or LPC species were increased by interventions. These findings were also confirmed in animal studies. The interesting point in animal studies was that high fat diet-induced elevation of PL tends to be normalized after intervention with dairy products enriched with milk-PL. Furthermore, in mice, intervention with yogurt or cheese did not impact serum or liver content of PL or PC. Conclusions Dairy products can influence the blood concentration of PC and LPC species in both rodents and humans without alteration of total PL and PC. Funding Sources Alberta Diabetes Institute.

scholarly journals Solitary skin-colored nodule on a child’s face

2022 ◽  
Vol 13 (1) ◽  
pp. 105-106
Author(s):  
Mariem Tabka ◽  
Refka Frioui ◽  
Taghrid Tlili ◽  
Nedia Fetoui ◽  
Amina Ounallah ◽  
...  
Keyword(s):  
Excisional Biopsy ◽  
Skin Tumor ◽  
White Background ◽  
Histopathological Analysis ◽  
Mandibular Angle ◽  
Her Family ◽  
Cowden’S Disease ◽  
Storiform Pattern ◽  
Dermal Collagen ◽  
Collagen Density

Sir, A healthy, six-year-old boy presented with a slowly grown dome-shaped nodule on the mandibular angle region present for two years. The patient’s past medical and family history were unremarkable. A physical examination revealed a solitary, 1.3 × 1 cm, firm, painless, flesh-colored tumor (Fig. 1). Dermoscopy showed branching, serpentine vessels on a pink background (Fig. 2a). These features disappeared when slight pressure was exerted on the dermoscope and the tumor exhibited a central, white, structureless area (Fig. 2b). An excisional biopsy was performed. A microscopic examination showed a well-circumscribed, paucicellular dermal tumor composed of eosinophilic collagen bundles separated by clefts and forming a storiform pattern. Scattered fibroblasts were found among the collagen bundles. The overlying epidermis was slightly flattened (Fig. 3). The diagnosis of solitary sclerotic fibroma was established. Sclerotic fibroma (SF), also known as storiform collagenoma, is a rare benign skin tumor. It usually manifests itself as an asymptomatic, slowly growing, white-to-skin-colored papule or nodule [1]. It was first described in patients with Cowden’s disease, yet may also occur sporadically [2]. There were no mucocutaneous features of Cowden’s disease (tricholemmomas, oral fibromas, acral keratoses, palmar pits, and gingival and palatal papules) in the patient and her family members. Dermatofibroma, the main differential diagnosis of SF, usually exhibits hyperplastic changes of the epidermis instead of atrophy, and the boundaries of the lesion are unclear [2]. Only two papers have been published describing the dermoscopic findings of SF, consisting of a white background with peripheral arborizing vessels [3]. A white background may be related to an increased dermal collagen density. It is also described in dermatofibroma, typically with a peripheral pigmentation network. Although dermoscopy may improve the clinical diagnosis of SF, histopathological analysis is required.

scholarly journals Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

2019 ◽  
Vol 2019 ◽  
pp. 1-19 ◽  
Author(s):  
Martina Balli ◽  
Jonathan Sai-Hong Chui ◽  
Paraskevi Athanasouli ◽  
Willy Antoni Abreu de Oliveira ◽  
Youssef El Laithy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Wound Repair ◽  
Molecular Mechanisms ◽  
Cell Model ◽  
Matrix Remodeling ◽  
Activator Protein 1 ◽  
Impaired Wound Healing ◽  
Beneficial Effects

Impaired wound healing and tissue regeneration have severe consequences on the patient’s quality of life. Micrograft therapies are emerging as promising and affordable alternatives to improve skin regeneration by enhancing the endogenous wound repair processes. However, the molecular mechanisms underpinning the beneficial effects of the micrograft treatments remain largely unknown. In this study, we identified the active protein-1 (AP-1) member Fos-related antigen-1 (Fra-1) to play a central role in the extracellular signal-regulated kinase- (ERK-) mediated enhanced cell migratory capacity of soluble micrograft-treated mouse adult fibroblasts and in the human keratinocyte cell model. Accordingly, we show that increased micrograft-dependent in vitro cell migration and matrix metalloprotease activity is abolished upon inhibition of AP-1. Furthermore, soluble micrograft treatment leads to increased expression and posttranslational phosphorylation of Fra-1 and c-Jun, resulting in the upregulation of wound healing-associated genes mainly involved in the regulation of cell migration. Collectively, our work provides insights into the molecular mechanisms behind the cell-free micrograft treatment, which might contribute to future advances in wound repair therapies.

Living cells of probiotic Bifidobacterium bifidum YIT 10347 detected on gastric mucosa in humans

2016 ◽  
Vol 7 (3) ◽  
pp. 319-326 ◽  
Author(s):  
H. Shibahara-Sone ◽  
A. Gomi ◽  
T. Iino ◽  
M. Kano ◽  
C. Nonaka ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Human Study ◽  
In Vitro Study ◽  
Probiotic Strain ◽  
Bifidobacterium Bifidum ◽  
Human Gastric Mucosa ◽  
Beneficial Effects ◽  
H Pylori

The probiotic strain Bifidobacterium bifidum YIT 10347 has been demonstrated to inhibit Helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in H. pylori positive patients. This study aimed to investigate the adhering activity and localisation of B. bifidum YIT 10347 to gastric cells and tissue in vitro, and in human in vivo to clarify the mechanism of its beneficial effects on the stomach. The in vitro study found the adhesion rate of B. bifidum YIT 10347 to human gastric epithelial cells was about 10 times higher than that of lactic acid bacteria and other bifidobacteria. In the human study, 5 H. pylori negative and 12 H. pylori positive subjects ingested milk fermented with B. bifidum YIT 10347. B. bifidum YIT 10347 cells were measured by RT-qPCR for in gastric biopsy samples. Living B. bifidum YIT 10347 cells were detected in the biopsy samples in H. pylori negative subjects (105 cells/g and 104 cells/g at 1 h and 2 h after ingestion, respectively) and H. pylori positive subjects (104 cells/g at 1 h after the ingestion). Moreover, immunostaining analysis of tissue sections found that B. bifidum YIT 10347 cells were located at the interstitial mucin layer of the stomach. These results suggest that cells of probiotic B. bifidum YIT 10347 adhered to the human gastric mucosa in a live state, and that the higher adhering activity of B. bifidum YIT 10347 to the gastric mucosa may be involved in its beneficial effects on the human stomach.

scholarly journals Novel 11β-hydroxysteroid dehydrogenase 1 inhibitors reduce cortisol levels in keratinocytes and improve dermal collagen content in human ex vivo skin after exposure to cortisone and UV

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171079 ◽  
Author(s):  
Stéphanie M. Boudon ◽  
Anna Vuorinen ◽  
Piero Geotti-Bianchini ◽  
Eliane Wandeler ◽  
Denise V. Kratschmar ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Hydroxysteroid Dehydrogenase ◽  
Collagen Content ◽  
Dermal Collagen ◽  
Cortisol Levels

scholarly journals Microsatellite polymorphism in the Heme oxygenase-1 gene promoter is associated with dermal collagen density in Japanese obese male subjects

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199994 ◽  
Author(s):  
Ai Ibuki ◽  
Takeo Minematsu ◽  
Mikako Yoshida ◽  
Shinji Iizaka ◽  
Masaru Matsumoto ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Gene Promoter ◽  
Microsatellite Polymorphism ◽  
Heme Oxygenase 1 ◽  
Dermal Collagen ◽  
Collagen Density ◽  
Male Subjects ◽  
Obese Male

scholarly journals Influence of lycopene and vitamin C from tomato juice on biomarkers of oxidative stress and inflammation

2007 ◽  
Vol 99 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Karin Jacob ◽  
María J. Periago ◽  
Volker Böhm ◽  
Gaspar Ros Berruezo
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Human Study ◽  
Thiobarbituric Acid ◽  
Tomato Juice ◽  
Protein Carbonyls ◽  
Beneficial Effects ◽  
Lipid Status ◽  
Biomarkers Of Oxidative Stress

A human study was carried out to investigate whether tomato juice, rich in natural lycopene and fortified with vitamin C, is able to reduce several biomarkers of oxidative stress and inflammation and whether the effect can be attributed to lycopene, vitamin C or any other micronutrient. Following a 2-week depletion phase, volunteers were assigned randomly to ingest either tomato juice with (LC) or without (L) vitamin C fortification for 2 weeks (daily dose 20·6 mg lycopene and 45·5/435 mg vitamin C). Plasma and urine were analysed for carotenoids and vitamin C, lipid status, antioxidant capacity, thiobarbituric acid reactive substances (TBARS) and 8-epi-PGF2α, protein carbonyls, cytokines IL-1β and TNFα and C-reactive protein (CRP). The consumption of tomato juice led to a reduction in total cholesterol levels (L: 157·6v. 153·2 mg/dl,P = 0·008; LC: 153·4v. 147·4 mg/dl,P = 0·002) and that of CRP (L: 315·6v. 262·3 μg/l,P = 0·017; LC: 319·2v. 247·1 μg/l,P = 0·001) in both groups. The vitamin C-fortified juice slightly raised the antioxidant capacity in urine and decreased TBARS in plasma and urine. All other markers were affected to a lesser extent or remained unchanged. Cholesterol reduction was correlated with lycopene uptake (P = 0·003), whereas the other effects could not be related with particular micronutrients. Any beneficial effects of tomato consumption for human health cannot be attributed only to lycopene and, as the additional supplementation with ascorbic acid indicates, a variety of antioxidants might be needed to optimize protection against chronic diseases.

Effects of severe caloric restriction from birth on the hearts of adult rats

2013 ◽  
Vol 38 (8) ◽  
pp. 879-885 ◽  
Author(s):  
Dirceu Sousa Melo ◽  
Tania Regina Riul ◽  
Elizabeth Adriana Esteves ◽  
Patrícia Lanza Moraes ◽  
Fernanda Oliveira Ferreira ◽  
...  
Keyword(s):  
Caloric Restriction ◽  
Ventricular Myocytes ◽  
Isolated Heart ◽  
Weight Ratio ◽  
Collagen Content ◽  
Aerobic Performance ◽  
Adult Rats ◽  
Beneficial Effects ◽  
Phosphorylated Akt ◽  
Intracellular Ca

There has been increasing evidence suggesting that a severe caloric restriction (SCR) (above 40%) has beneficial effects on the hearts of rats. However, most of the reports have focused on the effects of SCR that started in adulthood. We investigated the consequences of SCR on the hearts of rats subjected to SCR since birth (CR50). From birth to the age of 3 months, CR50 rats were fed 50% of the food that the ad libitum group (AL) was fed. Thereafter, a maximal aerobic test was performed to indirectly evaluate global cardiovascular function. Indices of contractility (+dT/dt) and relaxation (−dT/dt) were analyzed in isolated heart preparation, and cardiomyocyte diameter, number, density, and myocardium collagen content were obtained through histologic analysis. Ventricular myocytes were isolated, using standard methods to evaluate phosphorylated AKT levels, and Ca2+ handling was evaluated with a combination of Western blot analysis, intracellular Ca2+ imaging, and confocal microscopy. CR50 rats exhibited increased aerobic performance and cardiac function, as shown by the increase in ±dT/dt. Despite the smaller cardiomyocyte diameter, CR50 rats had an increased heart−body weight ratio, increased cardiomyocyte density and number, and similar levels of myocardium collagen content, compared with AL rats. AKT was hyperphosphorylated in cardiomyocytes from CR50 rats, and there were no significant differences in Ca2+ transient and SERCA2 levels in cardiomyocytes between CR50 and AL rats. Collectively, these observations reveal the beneficial effects of a 50% caloric restriction on the hearts of adult rats restricted since birth, which might involve cardiomyocyte AKT signaling.

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