scholarly journals Defective Repression of OLE3::LUC 1 (DROL1) is specifically required for the splicing of AT–AC-type introns in Arabidopsis

2020 ◽  
Author(s):  
Takamasa Suzuki ◽  
Tomomi Shinagawa ◽  
Tomoko Niwa ◽  
Hibiki Akeda ◽  
Satoki Hashimoto ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Abiotic Stress ◽  
Growth And Development ◽  
Growth Arrest ◽  
Comprehensive Analysis ◽  
Rna Seq ◽  
Plant Growth And Development ◽  
Abscisic Acid Signaling ◽  
A Subunit ◽  
Arabidopsis Homolog

AbstractAn Arabidopsis mutant named defective repression of OLE3::LUC 1 (drol1) was originally isolated as a mutant with defects in the repression of OLEOSIN3 (OLE3) after seed germination. In this study, we show that DROL1 is an Arabidopsis homolog of yeast DIB1, a subunit of U5 snRNP in the spliceosome, but comprises a subfamily specific to a certain class of eukaryotes. Comprehensive analysis of intron splicing by RNA-Seq analysis of drol1 mutants revealed reduced splicing of most of the minor introns with AT–AC dinucleotide termini. Thirty-nine genes, including those playing important roles in the response to abiotic stress, exhibited reduced splicing of AT–AC-type introns in drol1 mutants. In addition, drol1 mutant seedlings showed growth arrest, similar to that caused by the activation of abscisic acid signaling, as a result of reduced splicing of AT–AC-type introns in some genes. These results indicate that DROL1 is specifically involved in the splicing of introns with AT–AC termini, and splicing of these minor introns plays an important role in plant growth and development.

scholarly journals A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1247-1255 ◽  
Author(s):  
Eiji Nambara ◽  
Masaharu Suzuki ◽  
Suzanne Abrams ◽  
Donald R McCarty ◽  
Yuji Kamiya ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Growth And Development ◽  
Plant Hormone ◽  
The Other ◽  
Aba Signaling ◽  
Wild Type ◽  
Plant Growth And Development ◽  
Diverse Range ◽  
Abscisic Acid Signaling ◽  
Aba Insensitive

Abstract The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (−)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs. the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2, and a collection of new mutant alleles of the ABA-insensitive genes, ABI3, ABI4, and ABI5. The abi5 alleles are less sensitive to (+)-ABA than to (−)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals.

scholarly journals Genome-wide identification and expression analysis of the 14-3-3 gene family in soybean (Glycine max)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7950 ◽  
Author(s):  
Yongbin Wang ◽  
Lei Ling ◽  
Zhenfeng Jiang ◽  
Weiwei Tan ◽  
Zhaojun Liu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gene Family ◽  
Stress Responses ◽  
Growth And Development ◽  
Genomic Data ◽  
Evolutionary Analysis ◽  
Rna Seq ◽  
Plant Growth And Development ◽  
Genome Wide ◽  
Gene Structures

In eukaryotes, proteins encoded by the 14-3-3 genes are ubiquitously involved in the plant growth and development. The 14-3-3 gene family has been identified in several plants. In the present study, we identified 22 GmGF14 genes in the soybean genomic data. On the basis of the evolutionary analysis, they were clustered into ε and non-ε groups. The GmGF14s of two groups were highly conserved in motifs and gene structures. RNA-seq analysis suggested that GmGF14 genes were the major regulator of soybean morphogenesis. Moreover, the expression level of most GmGF14s changed obviously in multiple stress responses (drought, salt and cold), suggesting that they have the abilities of responding to multiple stresses. Taken together, this study shows that soybean 14-3-3s participate in plant growth and can response to various environmental stresses. These results provide important information for further understanding of the functions of 14-3-3 genes in soybean.

scholarly journals Genome wide survey, evolution and expression analysis of PHD finger genes reveal their diverse roles during the development and abiotic stress responses in Brassica rapa L.

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Intikhab Alam ◽  
Cui-Cui Liu ◽  
Hong-Liu Ge ◽  
Khadija Batool ◽  
Yan-Qing Yang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Plant Growth ◽  
Brassica Rapa ◽  
Expression Analysis ◽  
Stress Responses ◽  
Growth And Development ◽  
Gene Families ◽  
Plant Growth And Development ◽  
Genome Database ◽  
Phd Finger

Abstract Background Plant homeodomain (PHD) finger proteins are widely present in all eukaryotes and play important roles in chromatin remodeling and transcriptional regulation. The PHD finger can specifically bind a number of histone modifications as an “epigenome reader”, and mediate the activation or repression of underlying genes. Many PHD finger genes have been characterized in animals, but only few studies were conducted on plant PHD finger genes to this day. Brassica rapa (AA, 2n = 20) is an economically important vegetal, oilseed and fodder crop, and also a good model crop for functional and evolutionary studies of important gene families among Brassica species due to its close relationship to Arabidopsis thaliana. Results We identified a total of 145 putative PHD finger proteins containing 233 PHD domains from the current version of B. rapa genome database. Gene ontology analysis showed that 67.7% of them were predicted to be located in nucleus, and 91.3% were predicted to be involved in protein binding activity. Phylogenetic, gene structure, and additional domain analyses clustered them into different groups and subgroups, reflecting their diverse functional roles during plant growth and development. Chromosomal location analysis showed that they were unevenly distributed on the 10 B. rapa chromosomes. Expression analysis from RNA-Seq data showed that 55.7% of them were constitutively expressed in all the tested tissues or organs with relatively higher expression levels reflecting their important housekeeping roles in plant growth and development, while several other members were identified as preferentially expressed in specific tissues or organs. Expression analysis of a subset of 18 B. rapa PHD finger genes under drought and salt stresses showed that all these tested members were responsive to the two abiotic stress treatments. Conclusions Our results reveal that the PHD finger genes play diverse roles in plant growth and development, and can serve as a source of candidate genes for genetic engineering and improvement of Brassica crops against abiotic stresses. This study provides valuable information and lays the foundation for further functional determination of PHD finger genes across the Brassica species.

scholarly journals Understanding and exploiting autophagy signaling in plants

2017 ◽  
Vol 61 (6) ◽  
pp. 675-685 ◽  
Author(s):  
Henri Batoko ◽  
Yasin Dagdas ◽  
Frantisek Baluska ◽  
Agnieszka Sirko
Keyword(s):  
Abiotic Stress ◽  
Plant Growth ◽  
Growth And Development ◽  
Molecular Mechanisms ◽  
Regulatory Mechanisms ◽  
Catabolic Pathway ◽  
Plant Growth And Development ◽  
The Core ◽  
Important Pathway ◽  
Sessile Organisms

Autophagy is an essential catabolic pathway and is activated by various endogenous and exogenous stimuli. In particular, autophagy is required to allow sessile organisms such as plants to cope with biotic or abiotic stress conditions. It is thought that these various environmental signaling pathways are somehow integrated with autophagy signaling. However, the molecular mechanisms of plant autophagy signaling are not well understood, leaving a big gap of knowledge as a barrier to being able to manipulate this important pathway to improve plant growth and development. In this review, we discuss possible regulatory mechanisms at the core of plant autophagy signaling.

scholarly journals KEEP ON GOING, a RING E3 Ligase Essential for Arabidopsis Growth and Development, Is Involved in Abscisic Acid Signaling

2006 ◽  
Vol 18 (12) ◽  
pp. 3415-3428 ◽  
Author(s):  
Sophia L. Stone ◽  
Luis A. Williams ◽  
Lisa M. Farmer ◽  
Richard D. Vierstra ◽  
Judy Callis
Keyword(s):  
Abscisic Acid ◽  
Growth And Development ◽  
E3 Ligase ◽  
Abscisic Acid Signaling ◽  
Ring E3 Ligase ◽  
Ring E3

scholarly journals Identification and expression analysis of Shaker K+ channel genes in millet (Setaria italica) under abiotic stresses

2021 ◽  
Author(s):  
Ben Zhang ◽  
Hui Wang ◽  
Yue Guo ◽  
Xiaoxia Wang ◽  
Mengtao Lv ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Abiotic Stresses ◽  
Growth And Development ◽  
Abiotic Stress Tolerance ◽  
Foxtail Millet ◽  
K Channel ◽  
Quantitative Real Time Pcr ◽  
Plant Growth And Development ◽  
Transcription Level ◽  
Analysis Prediction

Potassium (K+) is one of the essential nutrients for plant, which is involved in plant growth and development and abiotic stress tolerance. The absorption and transport of K+ depends on Shaker K+ channels. Foxtail millet is a Poaceae crop with strong drought stress-tolerant. In this study, we identified ten Shaker K+ channel genes in foxtail millet. Phylogenetic analysis, prediction of conserved motif, and gene structure analysis classified these genes into five groups. The transcription level of these genes under different abiotic stress treatments (cold, heat, NaCl, PEG) and ABA treatment were analyzed by quantitative real-time PCR. Each gene displayed its own regulation pattern under different treatments, suggests these channels play important role in plant adaptation to different environment conditions.

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