scholarly journals Effect of telomerization on the growth patterns of adult cutaneous diploid fibroblasts

2021 ◽  
Author(s):  
Alexander A. Zgurskiy ◽  
Leonid Yu. Prokhorov
Keyword(s):  
Proliferative Activity ◽  
Doubling Time ◽  
Single Case ◽  
Control Culture ◽  
Growth Patterns ◽  
High Rate ◽  
Diploid Fibroblasts ◽  
Adult Men ◽  
Cell Divisions ◽  
Adult Fibroblasts

AbstractThe article describes effects of telomerization on proliferative activity in cultured cutaneous diploid fibroblasts obtained from an adult men aged 57 years. It is shown that the cells in the control culture initially divide quite actively, but starting from the 27th to the 40th passages, the rate of their division decreases and the time to reach the monolayer increases by about 2 times, and starting from the 42nd passage, the time to reach the monolayer increases by 4-10 times (up to 16-40 days). In a single case, an increase in the doubling time of control non-thelomerized cells was recorded up to 30 times (136 days). The introduction of the telomerase gene allowed cells maintain a high rate of cell division to about 45-47 passages. At the same time, the culture with the telomerase gene exceeds the control culture by 10 passages. Rate of growth the cells with telomerase gene after 48 and up to 57 passages slowed down by 10-18 times (up to 40-73 days). At the 47th passage in the control culture and at the 57th passage in the experimental culture, the cells practically stopped dividing and their number did not increase. The results show that the inclusion of the telomerase gene in adult fibroblasts does not always cancel the limit of cell divisions.

scholarly journals Modelling and analysis of COVID-19 epidemic in India

2020 ◽  
Author(s):  
Alok Tiwari
Keyword(s):  
Public Health ◽  
Doubling Time ◽  
Single Case ◽  
World Health Organisation ◽  
Public Health Emergency ◽  
Positive Case ◽  
World Health ◽  
The Public ◽  
First Case ◽  
The World

ABSTRACTCOVID-19 epidemic is declared as the public health emergency of international concern by the World Health Organisation in the second week of March 2020. This disease originated from China in December 2019 has already caused havoc around the world, including India. The first case in India was reported on 30th January 2020, with the cases crossing 6000 on the day paper was written. Complete lockdown of the nation for 21 days and immediate isolation of infected cases are the proactive steps taken by the authorities. For a better understanding of the evolution of COVID-19 in the country, Susceptible-Infectious-Quarantined-Recovered (SIQR) model is used in this paper. It is predicted that actual infectious population is ten times the reported positive case (quarantined) in the country. Also, a single case can infect 1.55 more individuals of the population. Epidemic doubling time is estimated to be around 4.1 days. All indicators are compared with Brazil and Italy as well. SIQR model has also predicted that India will see the peak with 22,000 active cases during the last week of April followed by reduction in active cases. It may take complete July for India to get over with COVID-19.

scholarly journals Origin of craniopharyngiomas: implications for growth pattern, clinical characteristics, and outcomes of tumor recurrence

2016 ◽  
Vol 125 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Yun Bao ◽  
Jun Pan ◽  
Song-tao Qi ◽  
Yun-tao Lu ◽  
Jun-xiang Peng
Keyword(s):  
Growth Pattern ◽  
Functional Outcomes ◽  
Growth Patterns ◽  
High Rate ◽  
Pituitary Function ◽  
Delayed Puberty ◽  
Neurosurgical Procedures ◽  
Type I ◽  
Imaging Features ◽  
Recurrent Tumors

OBJECT Craniopharyngiomas are associated with a high rate of recurrence. The surgical management of recurrent lesions has been among the most challenging neurosurgical procedures because of the craniopharyngioma's complex topographical relationship with surrounding structures. The aim of this study was to define the determinative role of the site of origin on the growth pattern and clinical features of recurrent craniopharyngiomas. METHODS The authors performed a retrospective analysis of 52 patients who had undergone uniform treatment by a single surgeon. For each patient, data concerning symptoms and signs, imaging features, hypothalamic-pituitary function, and recurrence-free survival rate were collected. RESULTS For children, delayed puberty was more frequent in the group with Type I (infradiaphragmatic) craniopharyngioma than in the group with Type TS (tuberoinfundibular and suprasellar extraventricular) lesions (p < 0.05). For adults, blindness was more frequent in the Type I group than in the Type TS group (p < 0.05). Nausea or vomiting, delayed puberty, and growth retardation were more frequent in children than in adults (p < 0.05). Overall clinical outcome was good in 48.07% of the patients and poor in 51.92%. Patients with Type TS recurrent tumors had significantly worse functional outcomes and hypothalamic function than patients with the Type I recurrent tumors but better pituitary function especially in children. CONCLUSIONS The origin of recurrent craniopharyngiomas significantly affected the symptoms, signs, functional outcomes, and hypothalamic-pituitary functions of patients undergoing repeated surgery. Differences in tumor growth patterns and site of origin should be considered when one is comparing outcomes and survival across treatment paradigms in patients with recurrent craniopharyngiomas.

scholarly journals Low proliferative level is a poor prognostic factor for colon adenocarcinoma

2014 ◽  
Vol 95 (3) ◽  
pp. 378-382
Author(s):  
G A Raskin ◽  
S V Petrov
Keyword(s):  
Prognostic Factor ◽  
Proliferative Activity ◽  
Single Case ◽  
Cancer Recurrence ◽  
Colon Adenocarcinoma ◽  
Tumor Stage ◽  
Ki 67 ◽  
Poor Prognostic Factor ◽  
Significant Difference ◽  
Primary Colon

Aim. To match up the proliferative activity of colon adenocarcinoma cells with tumor stage and survival rate. Methods. Ki-67 protein expression was evaluated by immunohistochemical methods in 217 patients with primary colon adenocarcinoma. After epitope retrieval and endogenous peroxidase inhibiting by 3% solution of hydrogen peroxide, histologic samples were stained by antibodies to Ki-67 protein (clone SP6, dilution 1:300) and polymer systemic detection with diaminobenzidine as a chromogenic substrate. Nuclear counterstain was performed using Mayer’s hematoxylin solution. Results. Assessment of colon adenocarcinoma proliferative activity showed a significant difference between the number of cases with high (70%) and relatively low (≤30%) proliferative levels in groups with metastatic cancers and non-metastatic tumors. In patients with no relapses, colon adenocarcinoma proliferative activity assessment showed proliferation level exceeding 70% in 21 (95%) out of 22 cases, in a single case proliferation level of 60% was found according to Ki-67, no cases of proliferation level lower than 50% was found. Statistical analysis showed that proliferative activity was significantly lower in patients with metastatic colon adenocarcinoma compared to cases of adenocarcinoma without metastases (p= 0.0019). We observe one clinical case of aggressive colon adenocarcinoma with omental, peritoneal, paraumbilical metastases in 28-year old patient, in whom proliferative activity by Ki-67 was measured as 20%. Conclusion. Low proliferative level in colon adenocarcinoma is a poor prognostic factor for possible metastasing and cancer recurrence.

scholarly journals Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1224-1227 ◽  
Author(s):  
AA Fauser ◽  
HA Messner
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Proliferative Activity ◽  
Growth Patterns ◽  
Short Term ◽  
Proliferative Rate ◽  
Normal Individuals ◽  
Short Term Exposure ◽  
Blood Specimens

Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

Sarcomatoid Renal Cell Carcinoma With Divergent Sarcomatoid Growth Patterns: A Case Report and Review of the Literature

2005 ◽  
Vol 129 (8) ◽  
pp. 1057-1060 ◽  
Author(s):  
Lin Li ◽  
Saul Teichberg ◽  
Joph Steckel ◽  
Qiang-hua Chen
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Single Case ◽  
Electron Microscopic Examination ◽  
Growth Patterns ◽  
World Health ◽  
Positive Staining ◽  
Electron Microscopic ◽  
Sarcomatoid Renal Cell Carcinoma

Abstract A 71-year-old woman who presented with left abdominal pain was found to have a noncalcified renal mass with a perisplenic extension on imaging studies. Histologically, the tumor showed predominantly malignant spindle cells with extensive osteoid and chondroid matrix production. Various growth patterns resembling rhabdomyosarcoma, malignant fibrous histiocytoma, and fibrosarcoma were also observed. Immunohistochemistry showed positive staining of the neoplastic cells for cytokeratin and focally positive staining for CD10 and CD117 (c-Kit). Electron microscopic examination revealed a poorly differentiated neoplasm with both mesenchymal and epithelial features. The tumor was diagnosed as a sarcomatoid renal cell carcinoma with overgrowth of the sarcomatoid component (World Health Organization: renal cell carcinoma, unclassified). To our knowledge, sarcomatoid renal cell carcinoma with such a broad morphologic phenotype in a single case has not been documented. Furthermore, the CD117 expression in a sarcomatoid renal cell carcinoma that was observed in this case merits further investigation.

Increasing cell membrane potential and GABAergic activity inhibits malignant hepatocyte growth

2003 ◽  
Vol 285 (1) ◽  
pp. G12-G19 ◽  
Author(s):  
D. Sun ◽  
Y. Gong ◽  
H. Kojima ◽  
G. Wang ◽  
E. Ravinsky ◽  
...  
Keyword(s):  
Western Blot ◽  
Mrna Expression ◽  
Cell Lines ◽  
Proliferative Activity ◽  
Malignant Cell ◽  
Growth Patterns ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Receptor Mrna ◽  
Gabaergic Activity

Increasing hepatocyte membrane potentials by augmenting GABAergic activity inhibits nonmalignant hepatocyte proliferative activity. The objectives of this study were to document 1) potential differences (PDs) of four malignant hepatocyte cell lines, 2) GABAA receptor mRNA expression in the same cell lines, and 3) effects of restoring malignant hepatocyte PDs to levels approximating those of resting, nonmalignant hepatocytes. Hepatocyte PDs were documented in nonmalignant and malignant (Chang, HepG2, HuH-7, and PLC/PRF/5) hepatocytes with a fluorescent voltage-sensitive dye and GABAA receptor expression by RT-PCR and Western blot analyses. Compared with nonmalignant human hepatocytes, all four malignant cell lines were significantly depolarized ( P < 0.0001, respectively). Only PLC/PRF/5 cells had detectable GABAA-β3 receptor mRNA expression and all cell lines were negative for GABAA-β3 receptor protein by Western blot analysis. Stable transfection of Chang cells with GABAA-β3 receptor cDNA resulted in significant increases in PD and decreases in proliferative activity as manifest by decreased [3H]thymidine and bromodeoxyurieine incorporation rates, 4-[3-(4-lodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate activity, a lower mitotic index, prolongation of cell-doubling times, and attenuated growth patterns compared with cells transfected with vector alone. Colony formation in soft agar and the number of abnormal mitoses were also significantly decreased in GABAA-β3 receptor transfected cells. The results of this study indicate 1) relative to healthy hepatocytes, malignant hepatocytes are significantly depolarized, 2) GABAA-β3 receptor expression is absent in malignant hepatocyte cell lines, and 3) increasing the PD of malignant hepatocytes is associated with less proliferative activity and a loss of malignant features.

Exploring the next generation socialisation to entrepreneurship in family businesses: insights from the Moroccan community

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Widad Ibouder ◽  
Alain Jean-Claude Fayolle ◽  
Abdenbi Louitri
Keyword(s):  
Single Case ◽  
Study Data ◽  
Family Businesses ◽  
High Rate ◽  
Next Generation ◽  
Content Type ◽  
Entrepreneurial Spirit ◽  
Exploratory Approach ◽  
Set Up ◽  
Rate Of Failure

Purpose In Morocco, due to the high rate of failure in generational transfers, many family businesses are threatened with disappearance. This paper aims to focus on entrepreneurship and seeks to understand how the family business maintains its entrepreneurial orientation through the family’s entrepreneurial socialisation of the next generation, which aims to prepare them for the role of family entrepreneur. The study aims both to describe the socialisation process and to understand the context necessary to place the young community in an entrepreneurial dynamic. Design/methodology/approach Using an exploratory approach, this paper favours the single case study; data were collected from five participants from both generations, then this study matches the interviews from the founding generation with those from the next generation; in addition to a triad at the employee level. Findings The analysis shows that the early participation alongside the founding generation in entrepreneurial processes, initiates a sense of entrepreneurship in the next generation and the confidence gradually builds up through the achievements of the latter; which, in turn, increases the possibility to set up innovative projects by giving the necessary autonomy to carry them out. Practical implications The document underlines the importance of establishing a culture of transmission to promote entrepreneurship amongst the young community to engage it in exploring and seeking new opportunities for development and innovation. Originality/value Studying the transmission of the entrepreneurial spirit through the prism of socialisation provides an understanding of the context necessary to place the next generation in an entrepreneurial dynamic.

Marrow culture studies in adult acute leukemia at presentation and during remission

Blood ◽  
1977 ◽  
Vol 49 (6) ◽  
pp. 903-912
Author(s):  
PC Vincent ◽  
R Sutherland ◽  
M Bradley ◽  
D Lind ◽  
FW Gunz
Keyword(s):  
Proliferative Activity ◽  
Growth Patterns ◽  
Leukemic Cells ◽  
Type B ◽  
Culture Studies ◽  
Abnormal Growth ◽  
Pattern Type ◽  
Intermittent Chemotherapy ◽  
Acute Nonlymphoblastic Leukemia ◽  
Marked Proliferation

Culture of bone marrow and/or blood cells in a semisolid agar system from 43 adults with acute nonlymphoblastic leukemia at first presentation showed two distinct growth patterns at 14 days. In 53% of patients cells failed to grow (type O), while in the remainder an abnormal growth pattern (type B) with small numbers of diffuse colonies and excessive numbers of cell clusters was seen. The response following chemotherapy was significantly better in the patients whose cells failed to grow. Serial culture studies, performed in 9 patients throughout remissions of 100–1112 days, which had been maintained by intermittent chemotherapy, showed wide fluctuations in proliferative activity. These ranged from no growth to marked proliferation with predominance of clusters and small numbers of diffuse colonies, indistinguishable from the type B pattern seen in 47% of patients at first presentation. The possibility is discussed that the periods of failure to grow, and/or those in which a type B pattern emerged, represented sporadic reactivation of leukemic cells.

scholarly journals Pluripotent hemopoietic progenitors (CFU-GEMM) in polycythemia vera: analysis of erythropoietin requirement and proliferative activity

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1224-1227 ◽  
Author(s):  
AA Fauser ◽  
HA Messner
Keyword(s):  
Polycythemia Vera ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Proliferative Activity ◽  
Growth Patterns ◽  
Short Term ◽  
Proliferative Rate ◽  
Normal Individuals ◽  
Short Term Exposure ◽  
Blood Specimens

Abstract Pluripotent hemopoietic progenitors (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. We have examined the erythropoietin requirements of CFU-GEMM-derived erythroid progeny in patients with polycythemia vera (PV) and studied their proliferative activity by short-term exposure to 3HTdR. Mixed colonies with erythroid components were observed in all bone marrow and peripheral blood samples from patients with PV that were cultured without addition of exogenous erythropoietin. This response is consistent with previously reported growth patterns for CFU-E and BFU-E. The frequency of mixed colonies increased regularly when erythropoietin was added to the cultures. Short-term exposure of peripheral blood specimens to 3HTdR prior to plating yielded a reduction of the plating efficiency by 20%- 70% when compared to cells that were not exposed to 3HTdR. The observation of cycling CFU-GEMM in PV contrasts with the usually quiescent behavior of CFU-GEMM in peripheral blood of normal individuals under steady-state conditions. These results support the view that the increased proliferative rate observed for CFU-GEMM may be responsible for the increased formation of blood cells in PV.

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