SARS-CoV-2 Spike S1-specific IgG kinetic profiles following mRNA- versus vector-based vaccination in the general Dutch population

mRNA- and vector-based vaccines are used at a large scale to prevent COVID-19. We compared Spike S1-specific (S1) IgG antibodies after vaccination with mRNA-based (Comirnaty, Spikevax) or vector-based (Janssen, Vaxzevria) vaccines, using samples from a Dutch nationwide cohort. mRNA vaccines induced faster inclines and higher S1 antibodies compared to vector-based vaccines in adults 18-64 years old (n=2,412). For all vaccines, one dose resulted in boosting of S1 antibodies in adults with a history of SARS-CoV-2 infection. For Comirnaty, two to four months following the second dose (n=196), S1 antibodies in adults aged 18-64 years old (436 BAU/mL, interquartile range: 328-891) were less variable and median concentrations higher compared to those in persons ≥80 years old (366, 177-743), but differences were not statistically significant (p>0.100). Nearly all participants seroconverted following COVID-19 vaccination, including the aging population. These data confirm results from controlled vaccine trials in a general population, including vulnerable groups.

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Seroprevalence of Sindbis virus and associated risk factors in northern Sweden

Epidemiology and Infection ◽

10.1017/s0950268813002239 ◽

2013 ◽

Vol 142 (7) ◽

pp. 1559-1565 ◽

Cited By ~ 15

Author(s):

C. AHLM ◽

M. ELIASSON ◽

O. VAPALAHTI ◽

M. EVANDER

Keyword(s):

Risk Factors ◽

Rural Communities ◽

Sindbis Virus ◽

Multiple Logistic Regression Analysis ◽

Northern Sweden ◽

Igg Antibodies ◽

History Of ◽

Specific Igg ◽

Associated Risk Factors ◽

Specific Igg Antibodies

SUMMARYMosquito-borne Sindbis virus (SINV) cause disease characterized by rash, fever and arthritis which often leads to long-lasting arthralgia. To determine the seroprevalence of SINV and associated risk factors in northern Sweden, a randomly selected population aged between 25 and 74 years were invited to join the MONICA study. Serum from 1611 samples were analysed for specific IgG antibodies. Overall, 2·9% had IgG against SINV. More men (3·7%) than women (2·0%) were SINV seropositive (P = 0·047) and it was more common in subjects with a lower educational level (P = 0·013) and living in small, rural communities (P < 0·001). Seropositivity was associated with higher waist circumference (P = 0·1), elevated diastolic blood pressure (P = 0·037), and history of a previous stroke (P = 0·011). In a multiple logistic regression analysis, adjusting for known risk factors for stroke, seropositivity for SINV was an independent predictor of having had a stroke (odds ratio 4·3, 95% confidence interval 1·4–13·0, P = 0·011).

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Seroprevalence of SARS-CoV-2-specific IgG antibodies in Kashmir, India, 7 months after the first reported local COVID-19 case: results of a population-based seroprevalence survey from October to November 2020

BMJ Open ◽

10.1136/bmjopen-2021-053791 ◽

2021 ◽

Vol 11 (9) ◽

pp. e053791

Author(s):

S Muhammad Salim Khan ◽

Mariya Amin Qurieshi ◽

Inaamul Haq ◽

Sabhiya Majid ◽

Javid Ahmad ◽

...

Keyword(s):

Sampling Design ◽

Final Analysis ◽

Population Based ◽

Secondary Outcome ◽

Igg Antibodies ◽

Serum Samples ◽

History Of ◽

Specific Igg ◽

Index Value ◽

Specific Igg Antibodies

ObjectivesWe designed a population-based survey in Kashmir to estimate the seroprevalence of SARS-CoV-2-specific IgG antibodies in the general population aged 18 years and above.SettingThe survey was conducted among 110 villages and urban wards across 10 districts in Kashmir from 17 October 2020 to 4 November 2020.ParticipantsIndividuals aged 18 years and above were eligible to be included in the survey. Serum samples were tested for the presence of SARS-CoV-2-specific IgG antibodies using the Abbott SARS-CoV-2 IgG assay.Primary and secondary outcome measuresWe labelled assay results equal to or above the cut-off index value of 1.4 as positive for SARS-CoV-2-specific IgG antibodies. Seroprevalence estimates were adjusted for the sampling design and assay characteristics.ResultsOut of 6397 eligible individuals enumerated, 6315 (98.7%) agreed to participate. The final analysis was done on 6230 participants. Seroprevalence adjusted for the sampling design and assay characteristics was 36.7% (95% CI 34.3% to 39.2%). Seroprevalence was higher among the older population. Among seropositive individuals, 10.2% (247/2415) reported a history of COVID-19-like symptoms. Out of 474 symptomatic individuals, 233 (49.2%) reported having been tested. We estimated an infection fatality rate of 0.034%.ConclusionsDuring the first 7 months of the COVID-19 epidemic in Kashmir Valley, approximately 37% of individuals were infected. The reported number of COVID-19 cases was only a small fraction of the estimated number of infections. A more efficient surveillance system with strengthened reporting of COVID-19 cases and deaths is warranted.

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Seroprevalence of SARS-CoV-2 specific IgG antibodies in District Srinagar, northern India – A cross-sectional study

PLoS ONE ◽

10.1371/journal.pone.0239303 ◽

2020 ◽

Vol 15 (11) ◽

pp. e0239303

Author(s):

S. Muhammad Salim Khan ◽

Mariya Amin Qurieshi ◽

Inaamul Haq ◽

Sabhiya Majid ◽

Arif Akbar Bhat ◽

...

Keyword(s):

Test Performance ◽

Cross Sectional Study ◽

Mitigation Measures ◽

Igg Antibodies ◽

Cross Sectional ◽

Essential Information ◽

Northern India ◽

History Of ◽

Specific Igg ◽

Specific Igg Antibodies

Background Prevalence of IgG antibodies against SARS-CoV-2 infection provides essential information for deciding disease prevention and mitigation measures. We estimate the seroprevalence of SARS-CoV-2 specific IgG antibodies in District Srinagar. Methods 2906 persons >18 years of age selected from hospital visitors across District Srinagar participated in the study. We tested samples for the presence of SARS-CoV-2 specific IgG antibodies using a chemiluminescent microparticle immunoassay-based serologic test. Results Age- and gender-standardized seroprevalence was 3.6% (95% CI 2.9% to 4.3%). Age 30–69 years, a recent history of symptoms of an influenza-like-illness, and a history of being placed under quarantine were significantly related to higher odds of the presence of SARS-CoV-2 specific IgG antibodies. The estimated number of SARS-CoV-2 infections during the two weeks preceding the study, adjusted for test performance, was 32602 with an estimated (median) infection-to-known-case ratio of 46 (95% CI 36 to 57). Conclusions The seroprevalence of SARS-CoV-2 specific IgG antibodies is low in the District. A large proportion of the population is still susceptible to the infection. A sizeable number of infections remain undetected, and a substantial proportion of people with symptoms compatible with COVID-19 are not tested.

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THE CONTENT OF ANTIBODIES TO THE SARS-COV-2 VIRUS IN STUDENTS OF A HIGHER EDUCATIONAL INSTITUTION

10.31089/978-5-6042929-2-1-2021-1-34-38 ◽

2021 ◽

Author(s):

V.T. Akhmetshina ◽

◽

L.G. Gizatullina ◽

L.M. Masyagutova

Keyword(s):

Educational Institution ◽

Dynamic Monitoring ◽

Igg Antibodies ◽

Average Value ◽

Population Immunity ◽

History Of ◽

Specific Igg ◽

Higher Educational ◽

Antibody Levels ◽

Specific Igg Antibodies

Abstract: Abstract: Today, a request is being formed to prevent the introduction of infection into organized groups by means of the formation of population immunity by methods of specific prevention. Purpose of the work: To carry out the determination of specific IgG antibodies to SARS-CoV-2 in students of a higher educational institution, to determine the number of students in need of vaccination. Material and research methods: The level of IgG antibodies to SARS-CoV-2 in the blood serum of students of a higher educational institution was analyzed. An analysis of the strength of immunity shows that the average value of the CP of positive samples is 11.3. A more significant diagnostic level of CP was revealed, indicating a pronounced tension of immunity in students with a history of pneumonia. Among those with a diagnostically significant positive CP level, more than a third of the examined subjects have lower IgG antibodies to SARS-CoV-2 than the average in this group. Consequently, these individuals require dynamic observation and monitoring of antibody levels in order to ensure timely vaccination. Thus, specific IgG antibodies to SARS-CoV-2 were detected in half of the examined students, which was 55%. It is this group that is subject to immediate vaccination before undergoing industrial practice. A third of students with low levels of antibodies to SARS-CoV-2 need dynamic monitoring of their content.

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Large-Scale Study of Antibody Titer Decay following BNT162b2 mRNA Vaccine or SARS-CoV-2 Infection

Vaccines ◽

10.3390/vaccines10010064 ◽

2021 ◽

Vol 10 (1) ◽

pp. 64

Author(s):

Ariel Israel ◽

Yotam Shenhar ◽

Ilan Green ◽

Eugene Merzon ◽

Avivit Golan-Cohen ◽

...

Keyword(s):

Large Scale ◽

Immune Protection ◽

Igg Antibodies ◽

Igg Antibody ◽

Exponential Decrease ◽

Previous Infection ◽

History Of ◽

Antibody Titers ◽

Antibody Levels ◽

Kinetics Of

Immune protection following either vaccination or infection with SARS-CoV-2 is thought to decrease over time. We designed a retrospective study, conducted at Leumit Health Services in Israel, to determine the kinetics of SARS-CoV-2 IgG antibodies following administration of two doses of BNT162b2 vaccine, or SARS-CoV-2 infection in unvaccinated individuals. Antibody titers were measured between 31 January 2021, and 31 July 2021 in two mutually exclusive groups: (i) vaccinated individuals who received two doses of BNT162b2 vaccine and had no history of previous infection with COVID-19 and (ii) SARS-CoV-2 convalescents who had not received the vaccine. A total of 2653 individuals fully vaccinated by two doses of vaccine during the study period and 4361 convalescent patients were included. Higher SARS-CoV-2 IgG antibody titers were observed in vaccinated individuals (median 1581 AU/mL IQR [533.8–5644.6]) after the second vaccination than in convalescent individuals (median 355.3 AU/mL IQR [141.2–998.7]; p < 0.001). In vaccinated subjects, antibody titers decreased by up to 38% each subsequent month while in convalescents they decreased by less than 5% per month. Six months after BNT162b2 vaccination 16.1% subjects had antibody levels below the seropositivity threshold of <50 AU/mL, while only 10.8% of convalescent patients were below <50 AU/mL threshold after 9 months from SARS-CoV-2 infection. This study demonstrates individuals who received the Pfizer-BioNTech mRNA vaccine have different kinetics of antibody levels compared to patients who had been infected with the SARS-CoV-2 virus, with higher initial levels but a much faster exponential decrease in the first group.

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Parvovirus B19 in Croatia: A Large-Scale Seroprevalence Study

Medicina ◽

10.3390/medicina57111279 ◽

2021 ◽

Vol 57 (11) ◽

pp. 1279

Author(s):

Tatjana Vilibic-Cavlek ◽

Irena Tabain ◽

Branko Kolaric ◽

Klara Mihulja ◽

Lana Blazevic ◽

...

Keyword(s):

General Population ◽

Large Scale ◽

Parvovirus B19 ◽

Adult Population ◽

Hemodialysis Patients ◽

Enzyme Linked Immunosorbent Assay ◽

Age Group ◽

Igg Antibodies ◽

Serological Tests ◽

Recent Infection

Background and Objectives: Seroepidemiological studies indicate that parvovirus B19 circulates in all areas of the world, although with some differences. The aim of this study is to analyze the seroprevalence of parvovirus B19 in the Croatian population. Materials and Methods: From 2010 to 2021, 1538 serum samples from different populations were tested for the presence of parvovirus B19 IgM/IgG antibodies. Serological tests were performed using a commercial enzyme-linked immunosorbent assay. Results: IgG antibodies were detected in 986/64.1% of participants with differences (p < 0.001) among the following population groups: 42.4% of children and adolescents, 67.1% of the adult general population, 66.7% of hemodialysis patients, and 65.6% of liver transplant recipients. Seroprevalence increased with age, from 30.0% in the 6 months–9 years age group to 69.0% in the 40–49 years age group, and remained stable thereafter (68.8–73.3%). There was no difference in the seropositivity among males (66.1%) and females (63.1%), as well as the place of residence (suburban/rural 63.9%, urban 64.1%). IgM antibodies (current/recent infection) were found in 61/4.0% of participants with the highest seropositivity in the youngest age group (11.1%). In pregnant women, seroprevalence was higher in women with an unfavorable obstetric history compared with a normal pregnancy (IgG 71.0% vs. 62.6%; IgM 6.5% vs. 2.4%), but these differences were not significant. Logistic regression showed that the adult population had almost three times higher risk of IgG seropositivity compared to children/adolescents (general population OR = 2.777, 95% CI = 2.023–3.812; hemodialysis patients OR = 2.586, 95% CI = 1.531–4.367; and transplant patients OR = 2.717, 95% CI = 1.604–4.603). A one-year increase in age increased the risk of IgG seroprevalence (OR = 1.017; 95% CI = 1.011–1.022). Conclusions: Older age was the main risk factor for IgG seropositivity. Hemodialysis and organ transplantation seem unrelated to the increased parvovirus B19 seroprevalence. The role of parvovirus B19 in the etiology of TORCH infections needs to be studied further.

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ToxocaraSeroprevalence in Patients with Idiopathic Parkinson's Disease: Chance Association or Coincidence?

BioMed Research International ◽

10.1155/2013/685196 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 9

Author(s):

Tuncay Çelik ◽

Yüksel Kaplan ◽

Eser Ataş ◽

Derya Öztuna ◽

Said Berilgen

Keyword(s):

Toxoplasma Gondii ◽

Parkinson Disease ◽

Causal Relation ◽

Clinical History ◽

Toxocara Canis ◽

Igg Antibodies ◽

History Of ◽

Specific Igg ◽

Genetic And Environmental Factors ◽

Specific Igg Antibodies

Most cases of idiopathic Parkinson disease (IPD) are believed to be due to a combination of genetic and environmental factors. The purpose of this study is to investigate the relationship between toxocariasis and Parkinson disease (PD). Patients were selected from people who were admitted to the Movement Disorders Branch, Neurology Department of Elazığ University Faculty of Medicine Elazığ, Turkey. We studied specific IgG antibodies againstToxocara canis(T. canis) in 50 patients with idiopathic Parkinson and 50 healthy volunteers. We investigated the clinical history of three patients infected withT. canis. We also studied specific IgG antibodies againstToxoplasma gondiiin these groups. Antibodies anti-Toxocara caniswere found in 3 idiopathic PD (6%) (P=0.121) and antibody titer was not found in control. A patient had history of the presence of dog in current dog ownership. We did not detect any statistically significant association betweenT. canisand IPD. But, we believe that further comprehensive studies are required for understanding whether there is a causal relation between toxocariasis and PD. We didn’t find possible association betweenToxoplasma gondiiand IPD (P=0.617).

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The Habitual Diet of Dutch Adult Patients with Eosinophilic Esophagitis Has Pro-Inflammatory Properties and Low Diet Quality Scores

Nutrients ◽

10.3390/nu13010214 ◽

2021 ◽

Vol 13 (1) ◽

pp. 214

Author(s):

Marlou L. A. de Kroon ◽

Simone R. B. M. Eussen ◽

Bridget A. Holmes ◽

Lucien F. Harthoorn ◽

Marijn J. Warners ◽

...

Keyword(s):

General Population ◽

Eosinophilic Esophagitis ◽

Diet Quality ◽

Saturated Fat ◽

Adult Patients ◽

Dietary Intakes ◽

Dutch Population ◽

Dietary Fiber Intake ◽

Habitual Diet ◽

General Dutch Population

We determined the nutritional adequacy and overall quality of the diets of adult patients with eosinophilic esophagitis (EoE). Dietary intakes stratified by sex and age were compared to Dietary Reference Values (DRV). Overall diet quality was assessed by two independent Diet-Quality-Indices scores, the PANDiet and DHD-index, and compared to age- and gender-matched subjects from the general population. Lastly, food and nutrient intakes of EoE patients were compared to intakes of the general population. Saturated fat intake was significantly higher and dietary fiber intake significantly lower than the DRV in both males and females. In males, the DRV were not reached for potassium, magnesium, selenium, and vitamins A and D. In females, the DRV were not reached for iron, sodium, potassium, selenium, and vitamins A, B2, C and D. EoE patients had a significantly lower PANDiet and DHD-index compared to the general population, although the relative intake (per 1000 kcal) of vegetables/fruits/olives was significantly higher (yet still up to 65% below the recommended daily amounts) and alcohol intake was significantly lower compared to the general Dutch population. In conclusion, the composition of the habitual diet of adult EoE patients has several pro-inflammatory and thus unfavorable immunomodulatory properties, just as the general Dutch population, and EoE patients had lower overall diet quality scores than the general population. Due to the observational character of this study, further research is needed to explore whether this contributes to the development and progression of EoE.

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Level of SARS-CoV-2 IgG antibodies after two doses CoronaVac vaccine: Primarily report

10.21203/rs.3.rs-388073/v1 ◽

2021 ◽

Author(s):

Umut Binay ◽

Faruk Karakeçili ◽

Orçun Barkay ◽

Özlem Gül ◽

Cuma Mertoğlu

Keyword(s):

University Hospital ◽

Spike Protein ◽

Effective Vaccine ◽

Igg Antibodies ◽

Fluorescent Assay ◽

Cross Sectional ◽

The People ◽

History Of ◽

Specific Igg ◽

Igg Level

Abstract Background: It is necessary to use an effective vaccine to end the COVID-19 pandemic. CoronaVac vaccine is used in our country and we aimed to examine the level of antibody development after the second dose.Methods: This is a retrospective, cross-sectional research. The data of the people, who applied to a university hospital between January and March 2021, were analyzed. Those who had SARS-CoV-2 IgG and IgM measurement in the previous two weeks before the CoronoVac vaccine, and those who were both found negative and who had SARS-CoV-2 IgG and IgM measurement after the second dose of CoronaVac vaccine were included in the research. SARS-CoV-2 IgG/IgM were measured by VIDAS® (BioMérieux, Marcy-l'Etoile, France) device for the detection of spike protein specific IgG/IgM of SARS-CoV-2 in human serum with ELFA (Enzyme Linked Fluorescent Assay) technique.Results: 75 people were included in this research. It was found that the individuals had SARS-CoV-2 IgG and IgM measurements between 14 and 21 days after the first dose of CoronaVac vaccine. It was observed that 12% (n = 9) of the cases had a history of COVID-19. The rate of positivity for SARS CoV-2 IgG level after vaccination was 100%.Conclusions: It can be said that two doses of CoronaVac vaccine create an effective humoral immunity.

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