The role of antifungal activity of ethyl acetate extract from Artemisia argyi on Verticillium dahliae

2021 ◽  
Author(s):  
Yunhan Wang ◽  
Jinxin Li ◽  
Qiaohuan Chen ◽  
Jia Zhou ◽  
Jiawei Xu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Verticillium Dahliae ◽  
Ethyl Acetate Extract ◽  
Artemisia Argyi

scholarly journals The Search for Antifungals from Amazonian Trees: A Bio-Inspired Screening

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000
Author(s):  
Charlie Basset ◽  
Véronique Eparvier ◽  
Laila S. Espindola
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Broad Spectrum ◽  
Tree Species ◽  
French Guiana ◽  
Antifungal Agents ◽  
Ethyl Acetate Extract ◽  
Natural Durability ◽  
Anti Fungal ◽  
Amazonian Trees

The anti-fungal activity of 60 extracts from 15 tree species in the French Guiana rainforest against human and wood-rotting fungi was studied. In this way (+)-mopanol (1) was isolated from the ethyl acetate extract of Peltogyne sp. (Caesalpiniaceae) wood. This work demonstrated that (1) the natural durability of wood can indeed guide the search for antifungal agents, (2) that extracts selected in this bio-inspired process exhibit a broad spectrum of antifungal activity and (3) that the method allows for the isolation of strongly active antifungals.

scholarly journals Antibacterial and Antifungal Activity of Heartwood of Acacia catechu of Nepal

2012 ◽  
Vol 27 ◽  
pp. 94-99 ◽  
Author(s):  
Susan Joshi ◽  
Yagya Prasad Subedi ◽  
Shobha Kumari Paudel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Diethyl Ether Extract ◽  
Pseudomonas Species ◽  
Acacia Catechu ◽  
Antibacterial And Antifungal

Antibacterial activity of different extracts of heartwood of Acacia catechu were tested against 11 species of bacteria and antifungal activity were tested against four species of fungi. Among bacteria species highest zone of inhibition (ZOI) was measured against Pseudomonas species by diethyl ether extract, likewise among fungal organism highest zone of inhibition (ZOI) was measured against Fusarium oxysporim by ethyl acetate extract. No extracts showed activity against Salmonella typhi, Salmonella paratyphi, Escherichia coli and Proteus mirabilis. The minimum bactericidal concentration (MBC) of ethyl acetate extract was evaluated against Bacillus subtilis, Klebsiella pneumonia, Staphylococus aureus and Shigella species.DOI: http://dx.doi.org/10.3126/jncs.v27i1.6667 J. Nepal Chem. Soc., Vol. 27, 2011 94-99 

Protective Role of Terminalia superba Ethyl Acetate Extract Against Oxidative Stress in Type 2 Diabetes

Pharmacologia ◽  
2011 ◽  
Vol 2 (12) ◽  
pp. 355-361
Author(s):  
Ngueguim Tsofack Florence ◽  
Tabi Nkoulou Thierry ◽  
Dzeufiet Djomeni Paul Desir ◽  
Tsala David Emeryb ◽  
Dongmo Alain Blaise ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Protective Role ◽  
Terminalia Superba

scholarly journals Quantification of Lupeol as Secondary Metabolite by HPTLC Technique and Assessment of Antimicrobial Potential of Ethyl Acetate Extract of Betula alnoides Bark

2021 ◽  
Vol 37 (2) ◽  
pp. 426-432
Author(s):  
Shahbaz Khan ◽  
Harpreet Singh ◽  
Arun K Mishra ◽  
Najam Ali Khan
Keyword(s):  
Antifungal Activity ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Antimicrobial Potential ◽  
Betula Alnoides ◽  
Layer Chromatography

The present work includes extraction of Betula alnoides bark using ethyl acetate as a solvent, preliminary phytochemical test, quantification of phytochemicals and quantification of lupeol in Betula alnoides by High Performance Thin Layer Chromatography (HPTLC) instrument along with the assessment of the antimicrobial potential of Ethyl Acetate Extract (EAE). The marc obtained after defatting of the coarsely powdered crude drug in Petroleum ether (60-80) was extracted using ethyl acetate. Afterward, preliminary phytochemical tests were done. For High Performance Thin Layer Chromatography (HPTLC), the solvent used was n-hexane: ethyl acetate (8:2 v/v) and scanning was performed at wavelength 254 nm. EAE was screened for antimicrobial potential. The extraction yield was 3.45% w/w. The result of the phytochemical analysis confirmed the presence of some important phytochemicals in EAE. A clear and resolved peak of lupeol was observed at Rf 0.61. The developed method was validated as per ICH guidelines. The concentration (%) of the marker compound (lupeol) was found to be 0.0168. Disk diffusion method using Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis as bacterial strains and Candida albicans, Aspergillus flavus and Epidermophyton floccosum as fungal strains against ciprofloxacin (for antibacterial activity) and fluconazole (for antifungal activity) as standard drugs was employed. The finding suggested that EAE possess significant antibacterial and antifungal activity when comparison was made with standard drugs. The proposed elucidated mechanism behind this action may be due to the presence of triterpenoids in EAE.

scholarly journals Antibacterial and antifungal activity of Moringa oleifera stem bark

2013 ◽  
pp. 109-117 ◽  
Author(s):  
Md Shafiqur Rahman ◽  
Laila Zerin ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Stem Bark ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

Petroleum ether, chloroform, ethyl acetate and carbon tetrachloride extracts of Moringa oleifera stem bark were studied for their antimicrobial activities against eleven human pathogenic bacteria (Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae) and four human pathogenic fungi (Aspergillus niger, A. ochraceus, A. ustus and Candida albicans) using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extracts exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extract exhibited the largest zone of inhibition (21 mm in diameter with 2000 mg/disc extract) against S. sonnei. The highest inhibition of fungal radial mycelial growth (52. 00% with 100 mg extract/ml medium) was recorded against C. albicans with ethyl acetate extract. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against B. megaterium, S. dysenteriae, V. cholerae and E. coli. For fungi, the lowest MIC was 500 mg/ml against C. albicans with crude extract of ethyl acetate. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13411 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):109-117, 2008

scholarly journals Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

2016 ◽  
Vol 19 (1) ◽  
pp. 37-43
Author(s):  
Md Anwarul Haque ◽  
Ashish Kumar Sarker ◽  
Md Ajijur Rahman ◽  
Md Aktar Uzzaman Chouduri ◽  
Md Anwar Ul Islam
Keyword(s):  
Antifungal Activity ◽  
Mortality Rate ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Small Scale ◽  
Dilution Method ◽  
Streptomyces Sp

To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50µg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40µg/ml and more where as LC50 was found to be 6.61µg/ml.Bangladesh Pharmaceutical Journal 19(1): 37-43, 2016

scholarly journals Antibacterial and antifungal activity of crude extracts of Plumeria rubra L.

2013 ◽  
pp. 87-94
Author(s):  
Laila Jarin ◽  
Md Shafiqur Rahman ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts ◽  
Plumeria Rubra

Petroleum ether, carbon tetrachloride, chloroform and ethyl acetate extracts of Plumeria rubra leaves were studied for their antimicrobial activities against eleven human pathogenic bacteria, viz., Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae and four human pathogenic fungi, viz., Aspergillus niger, A. ochraceus, A. ustus and Candida albicans using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extract exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extracts exhibited the largest zone of inhibition (25 mm in diameter with 2000 mg/disc extract) against E. coli. The highest inhibition of fungal radial mycelial growth (62.00% with 100 mg extract/ml medium) was recorded against A. ustus with ethyl acetate extract. The MICs were determined by broth macrodilution technique. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against E. coli. However, for fungi the lowest MIC was 500 mg/ml against A. ustus with the same extract. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13409 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):87-94, 2008

scholarly journals ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS

2017 ◽  
Vol 9 (7) ◽  
pp. 140
Author(s):  
Anto Suganya Regis ◽  
Jeya Jothi Gabriel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
High Concentration ◽  
Disc Diffusion Method ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Methanol Extracts

Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential extraction method using solvents hexane, ethyl acetate and methanol based on their polarity. The antifungal activity of the extracts was tested against 12 opportunistic fungal strains by disc diffusion method. Minimum inhibitory concentration (MIC) was determined using microtiter plate method.Results: The hexane, ethyl acetate and methanol extracts showed significant antifungal activities. The highest antifungal activity was recorded for ethyl acetate extract of C. axillaris. In disc diffusion method at high concentration (5 mg/ml), the ethyl acetate extract exhibited the zone of inhibition>30 mm against C. krusei, mentagrophytes, Scopulariopsis sp. and B. cinerea. In MIC the ethyl acetate extract inhibited the growth of T. mentagrophytes, Scopulariopsis sp., B. cinerea in its low dose (0.031 mg/ml). The hexane, ethyl acetate and methanol extracts of C. axillaris did not show activity against M. gypseum, T. rubrum and E. floccosum.Conclusion: This is the first report for the antifungal efficacy of C. axillaris. The results proved that the extracts of C. axillaris have high potential antifungal principles which could fight against the opportunistic and multidrug resistant fungal strains.

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