RhNAC3, a stress-associated NAC transcription factor, has a role in dehydration tolerance through regulating osmotic stress-related genes in rose petals

AbstractPetals and leaves share common evolutionary origins but have different phenotypic characteristics, such as the absence of stomata in the petals of most angiosperm species. Plant NAC transcription factor, NAP, is involved in ABA responses and regulates senescence-associated genes, and especially those that affect stomatal movement. However, the regulatory mechanisms and significance of NAP action in senescing astomatous petals is unclear. A major limiting factor is failure of flower opening and accelerated senescence. Our goal is to understand the finely regulatory mechanism of dehydration tolerance and aging in rose flowers. We functionally characterized RhNAP, an AtNAP-like transcription factor gene that is induced by dehydration and aging in astomatous rose petals. Cytokinins (CKs) are known to delay petal senescence and we found that a cytokinin oxidase/dehydrogenase gene 6 (RhCKX6) shares similar expression patterns with RhNAP. Silencing of RhNAP or RhCKX6 expression in rose petals by virus induced gene silencing markedly reduced petal dehydration tolerance and delayed petal senescence. Endogenous CK levels in RhNAP- or RhCKX6-silenced petals were significantly higher than those of the control. Moreover, RhCKX6 expression was reduced in RhNAP-silenced petals. This suggests that the expression of RhCKX6 is regulated by RhNAP. Yeast one-hybrid experiments and electrophoresis mobility shift assays showed that RhNAP binds to the RhCKX6 promoter in heterologous in vivo system and in vitro, respectively. Furthermore, the expression of putative signal transduction and downstream genes of ABA-signaling pathways were also reduced due to the repression of PP2C homolog genes by RhNAP in rose petals. Taken together, our study indicates that the RhNAP/RhCKX6 interaction represents a regulatory step enhancing dehydration tolerance in young rose petals and accelerating senescence in mature petals in a stomata-independent manner.

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Potato NAC Transcription Factor StNAC053 Enhances Salt and Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms22052568 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2568

Author(s):

Qi Wang ◽

Cun Guo ◽

Zhiyuan Li ◽

Jinhao Sun ◽

Zhichao Deng ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Stress Responses ◽

Nac Transcription Factor ◽

Transactivation Domain ◽

Wild Type ◽

Gfp Fusion Protein ◽

Salt And Drought Stress ◽

Stress Related Genes ◽

Nac Family

The NAC (NAM, ATAF1/2, and CUC2) transcription factors comprise one of the largest transcription factor families in plants and play important roles in stress responses. However, little is known about the functions of potato NAC family members. Here we report the cloning of a potato NAC transcription factor gene StNAC053, which was significantly upregulated after salt, drought, and abscisic acid treatments. Furthermore, the StNAC053-GFP fusion protein was found to be located in the nucleus and had a C-terminal transactivation domain, implying that StNAC053 may function as a transcriptional activator in potato. Notably, Arabidopsis plants overexpressing StNAC053 displayed lower seed germination rates compared to wild-type under exogenous ABA treatment. In addition, the StNAC053 overexpression Arabidopsis lines displayed significantly increased tolerance to salt and drought stress treatments. Moreover, the StNAC053-OE lines were found to have higher activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) under multiple stress treatments. Interestingly, the expression levels of several stress-related genes including COR15A,DREB1A, ERD11, RAB18, ERF5, and KAT2, were significantly upregulated in these StNAC053-overexpressing lines. Taken together, overexpression of the stress-inducible StNAC053 gene could enhance the tolerances to both salt and drought stress treatments in Arabidopsis, likely by upregulating stress-related genes.

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The durum wheat NAC transcription factor TtNAC2A enhances drought stress tolerance in Arabidopsis

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2021.104439 ◽

2021 ◽

Vol 186 ◽

pp. 104439

Author(s):

Dhawya Mergby ◽

Moez Hanin ◽

Mohammed Najib Saidi

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Durum Wheat ◽

Stress Tolerance ◽

Nac Transcription Factor ◽

Drought Stress Tolerance

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NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms22136952 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6952

Author(s):

Mingxin Yu ◽

Junling Liu ◽

Bingshuai Du ◽

Mengjuan Zhang ◽

Aibin Wang ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Stress Response ◽

Transcriptional Activation ◽

Dominant Role ◽

State Level ◽

Energy Conversion Efficiency ◽

Nac Transcription Factor ◽

Wild Plants ◽

Transgenic Lines

NAC (NAM, ATAF1/2, and CUC2) transcription factors are ubiquitously distributed in eukaryotes and play significant roles in stress response. However, the functional verifications of NACs in Picea (P.) wilsonii remain largely uncharacterized. Here, we identified the NAC transcription factor PwNAC11 as a mediator of drought stress, which was significantly upregulated in P. wilsonii under drought and abscisic acid (ABA) treatments. Yeast two-hybrid assays showed that both the full length and C-terminal of PwNAC11 had transcriptional activation activity and PwNAC11 protein cannot form a homodimer by itself. Subcellular observation demonstrated that PwNAC11 protein was located in nucleus. The overexpression of PwNAC11 in Arabidopsis obviously improved the tolerance to drought stress but delayed flowering time under nonstress conditions. The steady-state level of antioxidant enzymes’ activities and light energy conversion efficiency were significantly increased in PwNAC11 transgenic lines under dehydration compared to wild plants. PwNAC11 transgenic lines showed hypersensitivity to ABA and PwNAC11 activated the expression of the downstream gene ERD1 by binding to ABA-responsive elements (ABREs) instead of drought-responsive elements (DREs). Genetic evidence demonstrated that PwNAC11 physically interacted with an ABA-induced protein—ABRE Binding Factor3 (ABF3)—and promoted the activation of ERD1 promoter, which implied an ABA-dependent signaling cascade controlled by PwNAC11. In addition, qRT-PCR and yeast assays showed that an ABA-independent gene—DREB2A—was also probably involved in PwNAC11-mediated drought stress response. Taken together, our results provide the evidence that PwNAC11 plays a dominant role in plants positively responding to early drought stress and ABF3 and DREB2A synergistically regulate the expression of ERD1.

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The Legume miR1514a modulates a NAC transcription factor transcript to trigger phasiRNA formation in response to drought

Journal of Experimental Botany ◽

10.1093/jxb/erw380 ◽

2016 ◽

pp. erw380 ◽

Cited By ~ 9

Author(s):

Guadalupe Sosa-Valencia ◽

Miguel Palomar ◽

Alejandra A. Covarrubias ◽

José L. Reyes

Keyword(s):

Transcription Factor ◽

Nac Transcription Factor

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TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis

BMC Plant Biology ◽

10.1186/s12870-015-0644-9 ◽

2015 ◽

Vol 15 (1) ◽

Cited By ~ 128

Author(s):

Quanjun Huang ◽

Yan Wang ◽

Bin Li ◽

Junli Chang ◽

Mingjie Chen ◽

...

Keyword(s):

Transcription Factor ◽

Drought Tolerance ◽

Transgenic Arabidopsis ◽

Nac Transcription Factor ◽

Salt And Drought Tolerance

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Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction

Eukaryotic Cell ◽

10.1128/ec.3.6.1544-1556.2004 ◽

2004 ◽

Vol 3 (6) ◽

pp. 1544-1556 ◽

Cited By ~ 17

Author(s):

Jade Mei-Yeh Lu ◽

Robert J. Deschenes ◽

Jan S. Fassler

Keyword(s):

Signal Transduction ◽

Transcription Factor ◽

Osmotic Stress ◽

Kinase Activity ◽

Mitogen Activated Protein Kinase ◽

Osmotic Response ◽

Mitogen Activated Protein ◽

Kinase Pathway

ABSTRACT Yeast Sln1p is an osmotic stress sensor with histidine kinase activity. Modulation of Sln1 kinase activity in response to changes in the osmotic environment regulates the activity of the osmotic response mitogen-activated protein kinase pathway and the activity of the Skn7p transcription factor, both important for adaptation to changing osmotic stress conditions. Many aspects of Sln1 function, such as how kinase activity is regulated to allow a rapid response to the continually changing osmotic environment, are not understood. To gain insight into Sln1p function, we conducted a two-hybrid screen to identify interactors. Mog1p, a protein that interacts with the yeast Ran1 homolog, Gsp1p, was identified in this screen. The interaction with Mog1p was characterized in vitro, and its importance was assessed in vivo. mog1 mutants exhibit defects in SLN1-SKN7 signal transduction and mislocalization of the Skn7p transcription factor. The requirement for Mog1p in normal localization of Skn7p to the nucleus does not fully account for the mog1-related defects in SLN1-SKN7 signal transduction, raising the possibility that Mog1p may play a role in Skn7 binding and activation of osmotic response genes.

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