Thermal Analysis Measurement of Gold Nanoparticle Interactions With Cell and Biomaterial

2012 ◽  
Author(s):  
Zhenpeng Qin ◽  
Neha Shah ◽  
Taner Akkin ◽  
Warren C. W. Chan ◽  
John C. Bischof
Keyword(s):  
Drug Delivery ◽  
Thermal Analysis ◽  
Gold Nanoparticles ◽  
Contrast Agents ◽  
Gold Nanoparticle ◽  
Magnetic Fluid ◽  
Superparamagnetic Nanoparticles ◽  
Functional Nanoparticles ◽  
Nanoparticle Interactions ◽  
Analysis Measurement

The rapidly evolving field of nanomedicine focuses on the design and application of multi-functional nanoparticles for diagnosis and treatment of diseases especially cancer1. Many of these nanomaterials are designed to serve as drug delivery or image contrast agents, or even to generate heat for hyperthermia (i.e. treatment), of cancer. Heating examples include gold nanoparticles (GNPs) for photothermal therapy3, and superparamagnetic nanoparticles for magnetic fluid hyperthermia4.

Gold nanoparticles and gold nanoparticle-conjugates for delivery of therapeutic molecules. Progress and challenges

2014 ◽  
Vol 2 (27) ◽  
pp. 4204-4220 ◽  
Author(s):  
I. Fratoddi ◽  
I. Venditti ◽  
C. Cametti ◽  
M. V. Russo
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Gold Nanoparticle ◽  
Drug Delivery Vehicles ◽  
Selective Targeting ◽  
Therapeutic Molecules ◽  
Delivery Vehicles

Gold nanoparticles and their conjugates as drug delivery vehicles for selective targeting of cancer cells.

scholarly journals Controlled delivery of gold nanoparticle-coupled miRNA therapeutics via an injectable self-healing hydrogel

Nanoscale ◽  
2021 ◽  
Author(s):  
Casper F. T. van der Ven ◽  
Mark W. Tibbitt ◽  
João Conde ◽  
Alain van Mil ◽  
Jesper Hjortnaes ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Disease Model ◽  
Controlled Delivery ◽  
Heart Valve Disease ◽  
Self Healing ◽  
Injectable Hydrogel ◽  
Delivery Platform ◽  
Mirna Therapeutics

A novel injectable hydrogel drug delivery platform introduces miRNA therapeutics coupled to gold nanoparticles to cells in a 3D bioprinted heart valve disease model.

Programmable assembly of gold nanoparticle nanoclusters and lattices

2020 ◽  
Vol 8 (31) ◽  
pp. 6810-6813
Author(s):  
Luyao Shen ◽  
Victor Pan ◽  
Haofei Li ◽  
Yunlong Zhang ◽  
Pengfei Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Metallic Nanoparticles

Deterministic assembly of metallic nanoparticles (e.g. gold nanoparticles) into prescribed configurations has promising applications in many fields such as biosensing and drug delivery.

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

Fluorescence biosensor for Salmonella typhimurium detection in food based on nano-self-assembly of alendronic acid modified upconversion and gold nanoparticles

2021 ◽  
Author(s):  
Min Chen ◽  
Leiqing Pan ◽  
K. Tu
Keyword(s):  
Gold Nanoparticles ◽  
Salmonella Typhimurium ◽  
Gold Nanoparticle ◽  
Alendronic Acid ◽  
Self Assembly ◽  
Upconversion Nanoparticles ◽  
Fluorescent Biosensor ◽  
Fluorescence Biosensor

A simple and quick responsive fluorescent biosensor for Salmonella typhimurium detection based on the recognition of aptamer coupled with alendronic acid (ADA)@upconversion nanoparticles (UCNPs) and gold nanoparticle (AuNPs) has been...

scholarly journals Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Metals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 472
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Morphological Properties ◽  
Phytochelatin Synthase ◽  
Rhizobium Tropici ◽  
Whole Cells ◽  
E Coli ◽  
Recombinant Cells

Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

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