The morphological changes in lymphoid organs and peripheral blood indicators in rats after peroral administration of gold nanoparticles

2016 ◽  
Author(s):  
A. B. Bucharskaya ◽  
S. S. Pakhomy ◽  
O. V. Zlobina ◽  
G. N. Maslyakova ◽  
O. V. Matveeva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Peripheral Blood ◽  
Morphological Changes ◽  
Lymphoid Organs ◽  
Peroral Administration

scholarly journals Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats

2016 ◽  
Vol 09 (01) ◽  
pp. 1640004 ◽  
Author(s):  
Alla B. Bucharskaya ◽  
Svetlana S. Pakhomy ◽  
Olga V. Zlobina ◽  
Galina N. Maslyakova ◽  
Olga V. Matveeva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Peripheral Blood ◽  
Morphological Changes ◽  
Cancer Diagnostics ◽  
Lymphoid Organs ◽  
Male Rats ◽  
Laboratory Animals ◽  
Peroral Administration ◽  
Proliferation And Differentiation ◽  
Quantitative Changes

At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention. Here, we report the effects of prolonged peroral administration of GNPs with different sizes (2, 15 and 50[Formula: see text]nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180–220[Formula: see text]g, gold nanospheres sizes 2, 15 and 50[Formula: see text]nm were administered orally for 15 days at a dosage of 190[Formula: see text][Formula: see text]g/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50[Formula: see text]nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and differentiation of immune cells indicate the presence of immunostimulation effect of GNPs.

The reversibility of morphological changes in the mesenteric lymph nodes after peroral administration of gold nanoparticles

2014 ◽  
Author(s):  
Olga V. Zlobina ◽  
Svetlana S. Pakhomy ◽  
Alla B. Bucharskaya ◽  
Irina O. Bugaeva ◽  
Galina N. Maslyakova ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lymph Nodes ◽  
Morphological Changes ◽  
Peroral Administration ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

scholarly journals The morphological changes in the internal organs of laboratory animals after prolonged oral administration of gold nanoparticles

2016 ◽  
Vol 09 (04) ◽  
pp. 1642004
Author(s):  
Alla B. Bucharskaya ◽  
Galina N. Maslyakova ◽  
Svetlana S. Pakhomy ◽  
Olga V. Zlobina ◽  
Irina O. Bugaeva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Morphological Changes ◽  
Drug Carriers ◽  
Laboratory Animals ◽  
Peroral Administration ◽  
Prolonged Administration ◽  
Internal Organs ◽  
Wide Range ◽  
Spleen And Lymph Nodes ◽  
Cancer Diagnosis And Therapy

Recently gold nanoparticles (GNPs) have been actively studied as photothermal converters, drug carriers, and imaging agents in a wide range of applications in cancer diagnosis and therapy. The prolonged peroral administration of GNPs in a range of sizes was performed to investigate the morphological changes and their reversibility in the internal organs of laboratory animals. In this study, GNPs with average diameters of 2[Formula: see text]nm, 15[Formula: see text]nm and 50[Formula: see text]nm were administered during 30 days, and the reversibility of morphological changes was investigated 14 days after administration. After the prolonged administration of GNPs, the severity of morphological changes in the liver, kidney, spleen and lymph nodes depended on the nanoparticle size. Specifically, 50[Formula: see text]nm nanoparticles caused the most pronounced dystrophic and necrobiotic effects, whereas the smallest 2[Formula: see text]nm nanoparticles caused proliferative changes. Most importantly, the development of pathological processes was reversible, as evidenced by the gradual restoration of the organ structure at 14 days after the end of GNPs administration.

scholarly journals Molecular Profiling and Gene Banking of Rabbit EPCs Derived from Two Biological Sources

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 366
Author(s):  
Jaromír Vašíček ◽  
Andrej Baláži ◽  
Miroslav Bauer ◽  
Andrea Svoradová ◽  
Mária Tirpáková ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Morphological Changes ◽  
Umbilical Vein ◽  
Differentiation Potential ◽  
Neuronal Markers ◽  
High Viability ◽  
Droplet Pcr ◽  
Passage Number ◽  
Umbilical Vein Endothelial Cells

Endothelial progenitor cells (EPCs) have been broadly studied for several years due to their outstanding regenerative potential. Moreover, these cells might be a valuable source of genetic information for the preservation of endangered animal species. However, a controversy regarding their characterization still exists. The aim of this study was to isolate and compare the rabbit peripheral blood- and bone marrow-derived EPCs with human umbilical vein endothelial cells (HUVECs) in terms of their phenotype and morphology that could be affected by the passage number or cryopreservation as well as to assess their possible neuro-differentiation potential. Briefly, cells were isolated and cultured under standard endothelial conditions until passage 3. The morphological changes during the culture were monitored and each passage was analyzed for the typical phenotype using flow cytometry, quantitative real–time polymerase chain reaction (qPCR) and novel digital droplet PCR (ddPCR), and compared to HUVECs. The neurogenic differentiation was induced using a commercial kit. Rabbit cells were also cryopreserved for at least 3 months and then analyzed after thawing. According to the obtained results, both rabbit EPCs exhibit a spindle-shaped morphology and high proliferation rate. The both cell lines possess same stable phenotype: CD14-CD29+CD31-CD34-CD44+CD45-CD49f+CD73+CD90+CD105+CD133-CD146-CD166+VE-cadherin+VEGFR-2+SSEA-4+MSCA-1-vWF+eNOS+AcLDL+ALDH+vimentin+desmin+α-SMA+, slightly different from HUVECs. Moreover, both induced rabbit EPCs exhibit neuron-like morphological changes and expression of neuronal markers ENO2 and MAP2. In addition, cryopreserved rabbit cells maintained high viability (>85%) and endothelial phenotype after thawing. In conclusion, our findings suggest that cells expanded from the rabbit peripheral blood and bone marrow are of the endothelial origin with a stable marker expression and interesting proliferation and differentiation capacity.

scholarly journals Pleurotus sajor-caju-Mediated Synthesis of Silver and Gold Nanoparticles Active against Colon Cancer Cell Lines: A New Era of Herbonanoceutics

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3091 ◽  
Author(s):  
Vivek K. Chaturvedi ◽  
Navneet Yadav ◽  
Neeraj K. Rai ◽  
Noura H. Abd Ellah ◽  
Raghvendra A. Bohara ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cell ◽  
Morphological Changes ◽  
Synthesis Method ◽  
Colon Cancer Cell ◽  
Similar Distribution ◽  
New Era ◽  
Silver And Gold Nanoparticles ◽  
Pleurotus Sajor Caju

Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active ‘phyto’ ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.

Abstract 1155: Quantification of circulating tumor cells in peripheral blood using EGFR-targeting gold nanoparticles

2010 ◽  
Author(s):  
Xu Wang ◽  
Ximei Qian ◽  
Jonathan J. Beitler ◽  
Zhuo Georgia Chen ◽  
Shuming Nie ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Peripheral Blood

The Influence of Long-Term Peroral Administration of Gold Nanoparticles with Various Sizes on the Liver, Spleen, and Lymph Nodes of Laboratory Rats and Their Progeny

2019 ◽  
Vol 126 (6) ◽  
pp. 681-686
Author(s):  
S. S. Pakhomy ◽  
A. B. Bucharskaya ◽  
G. N. Maslyakova ◽  
O. V. Zlobina ◽  
I. O. Bugaeva ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lymph Nodes ◽  
Peroral Administration ◽  
Laboratory Rats ◽  
Spleen And Lymph Nodes

scholarly journals Effects of Homozygosity of the nude (rnu) gene in an inbred strain of rats: Studies of lymphoid and non-lymphoid organs in different age groups of nude rats of LEW background at a stage in the gene transfer

1984 ◽  
Vol 18 (1) ◽  
pp. 7-14 ◽  
Author(s):  
H. P. Hougen ◽  
B. Klausen
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Genetic Background ◽  
Early Stage ◽  
Age Groups ◽  
Lymphoid Organs ◽  
Thymic Tissue ◽  
Nude Rats ◽  
Histological Appearance ◽  
Differential Counts

Several age groups of nude homozygous rnu/rnu and heterozygous rnu/+ rats of the same genetic background at an early stage of back-crossing (LEW/Mol) were compared as to body and organ weights, histological appearance and cell density of lymphoid organs, haematological values and differential counts of bone marrow and peripheral blood. No thymic tissue was found in the nude animals. 7-week-old nudes were smaller than control animals and had relatively larger non-lymphoid organs and cell-depleted peripheral lymphoid organs. Other age groups showed little difference. Peripheral blood of nude rats showed no signs of lymphopaenia in contrast with the findings in nude mice. The number of thoracic duct lymphocytes was, however, significantly smaller in all age groups of the nude rats, and the bone marrow tended to contain fewer lymphocytes.

Impaired Clonogenic Capacity Contributes to Bone Marrow Hypoplasia and Late Hematological Recovery after Zevalin in the Low-Grade NHL Patients.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4610-4610
Author(s):  
Wojciech Jurczak ◽  
Marta Szostek ◽  
Zbigniew Rudzki ◽  
Dorota Krochmalczyk ◽  
Joanna Wegrzyn ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Morphological Changes ◽  
Petri Dish ◽  
Relative Increase ◽  
Significant Inhibition ◽  
Low Grade ◽  
Bone Marrow Hypoplasia

Abstract Chemoimmunotherapy with Zevalin (anty CD20,90Y) is a new therapeutic approach in the resistant or relapsed low grade malignant lymphoma patients. The most important side effect observed in the up to date studies is related to transient peripheral blood cytopenias. In an attempt to evaluate the involved pathogenetic mechanisms of the above findings, the blood and bone marrow samples from 8 low grade NHL patients were prospectively tested for the peripheral blood count, the clonogenic capacity and the histopathology abnormalities (trephine biopsies) before and every 14 days after Zevalin administration until recovery. In the majority evaluated patients the mild thrombocytopenia (30-55000/μl) was observed 4 weeks after Zevalin therapy. Bone marrow mononuclear cells were cultured in methylcellulose assay using combination of recombinant CSF (IL-3, IL-6, stem cell factor, erythropoietin; Methocult, StemCell, Canada), at a concentration of 1x10^5 cells, in 35 mm gridded Petri dish and incubated at 37°C, in a humidified atmosphere of 5% CO2. The hematopoietic colonies (CFU-GM, BFU-E and CFU-GEMM) were evaluated on 14th d. of the culture. The in vitro CFU-Meg formation in the serum-free collagen-based semisolid culture (Megacult, StemCell, Canada) were also studied, stained after 12 days with a monoclonal antibody against CD 41. In the results of our studies the significant inhibition of colony formation was found already in 7th day for CFU-GM and BFU-E and starting from 14th day after Zevalin administration for CFU-Meg. In the subsequent cultures evaluated during 4–8 weeks after Zevalin therapy the three lineages hematopoietic clonogenic capacity returned to the pre-radiotherapy values. In the repeated trephine biopsis several morphological changes were observed: oedema of stromal cells, the relative increase of erythropoietic components with inverted proportion of granulo- to erythropoiesis. In conclusion, chemoimmunotherapy with Zevalin resulted in the reversible peripheral cytopenia parallely with transient damage to bone marrow hematopoiesis and in vitro clonogenic capacity.

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