The aim of the study is to determine the contribution of external and internal factors, such as the season, body mass index (BMI), age, sex, degree of AH, the formation of insufficiency and/or deficiency of 25 (OH)D in adolescents with arterial hypertension (AH). Materials and methods: the core group consisted of 87 adolescents with primary AH, which then were divided into two subgroups depending on BMI (Subgroup 1 – overweight and obese, Subgroup 2 – with normal body weight). The control group consisted of 23 adolescents with the phenomenon of «white coat hypertension» (WHT). The level of 25(OH)D in the blood serum was determined by immunoextraction with further with further quantitative determination by enzyme immunoassay using StarFax 2100 analyzer (USA). A DIA source Immuno assays S.A. test system. (Belgium) was used. All calculations were carried out according to the instruction in which the norm was 25(OH)D>/=30–100 ng/ml; insufficiency – 10–29 ng/ml; deficiency – > 10 ng/ml. Results: the level of 25(OH)D in peripheral blood fluctuated in a wide range – from 5,1 to 50,2 ng/ml, the median level of 25(OH)D was 17,8 ng/ml [12,2; 23,5]. According to the results of the study, only 4 (3,6%) children had a normal vitamin D level, 95 (86,4%) had vitamin D insufficiency and 11 (10%) had a deficiency. The median values of 25(OH)D in children of the comparison group were statistically significantly higher than in children with AH of the 1st and 2nd subgroups – 23,3 ng/ml [20,8; 26,0], 14,9 ng/ml [10,8; 19,5] and 17,1 ng/ml [11,8; 23,7], respectively, p=0,001. Conclusion: according to the results of the multivariate analysis, 25(OH)D in the observed adolescents depends on many factors, such as age (6,7%), gender differences (5,7%), etc. The seasons have the greatest influence on its level (62,3%) and BMI (21,4%), the least – AH (3,9%).