scholarly journals Validation of an In-House Assay for Cytomegalovirus Immunoglobulin G (CMV IgG) Avidity and Relationship of Avidity to CMV IgM Levels

2002 ◽  
Vol 9 (4) ◽  
pp. 824-827 ◽  
Author(s):  
Harry E. Prince ◽  
Amy L. Leber
Keyword(s):  
Pregnant Women ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Avidity ◽  
Cmv Infection ◽  
Capture Elisa ◽  
Igg Avidity ◽  
Relationship Of ◽  
The Relationship ◽  
Positive Results

ABSTRACT Measurement of cytomegalovirus (CMV)-specific immunoglobulin G (IgG) avidity has proven to be a powerful tool for distinguishing primary from nonprimary CMV infection. An in-house enzyme-linked immunosorbent assay (ELISA) for measuring CMV IgG avidity was validated using 84 sera from pregnant women who had recently seroconverted following primary CMV infection and 74 sera from individuals with past CMV infection (IgG-positive and IgM-negative profile). Of the 84 sera from pregnant women, 73 sera were collected within 120 days of the last IgG-negative sample, and 72 of these 73 sera (99%) exhibited an avidity index (AI) of <50%. In contrast, 71 of 74 (96%) sera from individuals with past CMV infection exhibited CMV AI values of >60%. Thus, low avidity in the in-house ELISA was defined as an AI of ⩽50%, whereas high avidity was defined as an AI of ⩾60%. In additional studies, the relationship between CMV IgG avidity and CMV IgM levels was examined using 64 CMV IgG-positive sera (time since seroconversion unknown) exhibiting equivocal or positive results in a CMV IgM capture ELISA (Diamedix). Of these 64 sera, 29 exhibited IgM index values of ⩾3.0, and 27 of these 29 (93%) exhibited low IgG avidity. A similar trend was observed when a subset of these 64 sera (n = 48) was tested in another CMV IgM capture ELISA (Trinity); of 18 sera with IgM index values of ⩾3.0, 17 (94%) exhibited low IgG avidity. These findings demonstrate the validity of an in-house ELISA for CMV IgG avidity and further show that strong reactivity of CMV IgG-positive sera in either of two CMV IgM capture assays is a reliable indicator of low CMV IgG avidity, and thus, recent CMV infection.

scholarly journals Immunoglobulin G Avidity in Diagnosis of Toxoplasmic Lymphadenopathy and Ocular Toxoplasmosis

1999 ◽  
Vol 6 (4) ◽  
pp. 514-518 ◽  
Author(s):  
Malgorzata Paul
Keyword(s):  
Pregnant Women ◽  
Immunoglobulin G ◽  
Large Range ◽  
Congenital Toxoplasmosis ◽  
Chronic Phase ◽  
Ocular Toxoplasmosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ocular Infection ◽  
High Avidity ◽  
Igg Avidity

ABSTRACT Traditional serological techniques have some limitations in evaluating the duration of Toxoplasma gondii infection in pregnant women, patients with lymphadenopathy, and older children suspected of having congenital toxoplasmosis. In these three groups of patients, two variants of T. gondii immunoglobulin G (IgG) avidity tests were used: an EIA Kit (Labsystems) and a noncommercial enzyme-linked immunosorbent assay specially elaborated in the laboratory. The avidity of specific IgG in sera from 23 patients with a known recently acquired infection (mainly pregnant women) was low (less than 30%), whereas that in sera from 19 patients with toxoplasmic lymphadenopathy of 3 weeks to 6 months in duration (mean, 8.3 weeks) covered a large range (between 0.2 and 57.8%; mean, 25.7%); high avidity results were observed for 10 of 19 patients (52.6%). The large range of IgG avidity in patients with toxoplasmic lymphadenopathy suggests various durations of infection in these patients, with a tendency for a chronic phase of toxoplasmosis. According to the avidity marker, five patients with lymphadenopathy for less than 3 months did not have a recent Toxoplasma infection. In 6 of 19 patients with lymphadenopathy (31.6%), low IgG avidity values persisted until 5 months after the first serological examination. In all four patients with a documented chronic course of Toxoplasma infection (6 months to 8 years after the first positive serology), high IgG avidity values were observed. Among sera from 10 children and young immunocompetent adults suspected of having ocular reactivation of congenital toxoplasmosis, all had high IgG avidity values (over 40%), suggesting congenitally acquired ocular infection rather than noncongenital infection. In conclusion, the avidity of IgG is a valuable marker of recent toxoplasmosis in pregnant women, suggests the duration of invasion in patients with lymphadenopathy, and may be helpful for differentiation between reactivation of congenital infection and recently acquired ocular toxoplasmosis in immunocompetent patients. A low IgG avidity does not always identify a recent case of toxoplasmosis, but a high IgG avidity can exclude primary infections of less than 5 months’ duration.

scholarly journals Detection of Acute and Chronic Toxoplasma gondii Infection among Women with History of Abortion in the Southwest of Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Jasem Saki ◽  
Maryam Zamanpour ◽  
Mahin Najafian ◽  
Niloofar Mohammadpour ◽  
Masoud Foroutan
Keyword(s):  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Umbilical Cord ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Avidity ◽  
Normal Delivery ◽  
Blood Samples ◽  
Igg Avidity ◽  
Avidity Test ◽  
History Of

Background. Toxoplasma gondii (T. gondii) is one of the most common intracellular protozoan parasites, which can infect humans and a wide range of mammals and birds. The current study is aimed at investigating the occurrence of T. gondii infection in women with a history of abortion in Khuzestan, Iran. Materials and Methods. A total of 480 women with an abortion history, as well as 200 pregnant women with a normal delivery, were examined in this study. The blood, placenta, and umbilical cord blood samples were assessed by the enzyme-linked immunosorbent assay (ELISA) and nested-polymerase chain reaction (PCR) assay. Results. Based on the results of ELISA assay, the prevalence of toxoplasmosis was 30.83% in women with a history of abortion (25.62% with T. gondii IgG and 5.20% with T. gondii IgM). According to the IgG avidity test, 60.16% of IgG-positive samples showed high avidity, while 27.64% showed low avidity. On the other hand, the prevalence of toxoplasmosis in women with a normal delivery was 23% (21.5% with T. gondii IgG and 1.5% with T. gondii IgM). According to the IgG avidity test, 81.39% of these women showed high avidity, while only 4.65% showed low avidity. Based on the nested-PCR method, T. gondii DNA was detected in 14.18% of blood samples, 4.69% of placental samples, and 1.34% of umbilical cord samples, collected from 148 seropositive women with a history of abortion. Besides, using this method, the parasite DNA was identified in 4.34% of blood samples, collected from 46 seropositive women with a normal delivery, but not in any of the umbilical cord or placenta samples. Conclusion. The present results showed that T. gondii infection contributes to abortion in Khuzestan Province, Iran. Therefore, it is essential to investigate toxoplasmosis in pregnant women, especially in those who are seronegative, using molecular and serological methods and inform them about their disease and the associated risks.

scholarly journals Role of Cytomegalovirus (CMV) IgG Avidity Testing in Diagnosing Primary CMV Infection during Pregnancy

2014 ◽  
Vol 21 (10) ◽  
pp. 1377-1384 ◽  
Author(s):  
Harry E. Prince ◽  
Mary Lapé-Nixon
Keyword(s):  
Pregnant Women ◽  
Primary Infection ◽  
Viral Reactivation ◽  
Published Data ◽  
Specific Method ◽  
High Avidity ◽  
Cmv Infection ◽  
Igg Avidity ◽  
Increased Risk

ABSTRACTThe risk of intrauterine transmission of cytomegalovirus (CMV) during pregnancy is much greater for women who contract primary CMV infection after conception than for women with evidence of infection (circulating CMV antibodies) before conception. Thus, laboratory tests that aid in the identification of recent primary CMV infection are important tools for managing the care of pregnant women suspected of having been exposed to CMV. CMV IgM detection is a sensitive marker of primary CMV infection, but its specificity is poor because CMV IgM is also produced during viral reactivation and persists following primary infection in some individuals. Studies conducted over the last 20 years convincingly demonstrate that measurement of CMV IgG avidity is both a sensitive and a specific method for identifying pregnant women with recent primary CMV infection and thus at increased risk for vertical CMV transmission. IgG avidity is defined as the strength with which IgG binds to antigenic epitopes expressed by a given protein; it matures gradually during the 6 months following primary infection. Low CMV IgG avidity is an accurate indicator of primary infection within the preceding 3 to 4 months, whereas high avidity excludes primary infection within the preceding 3 months. In this minireview, we summarize published data demonstrating the clinical utility of CMV IgG avidity results for estimating time since primary infection in pregnant women, describe commercially available CMV IgG avidity assays, and discuss some of the issues and controversies surrounding CMV IgG avidity testing during pregnancy.

scholarly journals Use of Dense Granule Antigen GRA6 in an Immunoglobulin G Avidity Test To Exclude Acute Toxoplasma gondii Infection during Pregnancy

2010 ◽  
Vol 17 (9) ◽  
pp. 1349-1355 ◽  
Author(s):  
Hossein Elyasi ◽  
Jalal Babaie ◽  
Hélène Fricker-Hidalgo ◽  
Marie-Pierre Brenier-Pinchart ◽  
Mehrak Zare ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Chronic Infection ◽  
Dense Granule ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Avidity ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Recent Infection ◽  
Igg Avidity

ABSTRACT The usefulness of a specific immunoglobulin G (IgG) avidity enzyme-linked immunosorbent assay (ELISA) based on recombinant GRA6 antigen for distinguishing between acute and chronic Toxoplasma infection was investigated. Two sets of serum samples obtained from pregnant women with acute, chronic, or no Toxoplasma infection collected in France and Iran were used. Among the French subjects, 19 of 20 (95%) women who experienced seroconversion during the past 4 months before sampling displayed low-avidity IgG antibodies against GRA6, while all 17 (100%) women with chronic infection had high-avidity antibodies. When the Euroimmun IgG avidity ELISA was used, 15 of 19 (78.9%) recently infected women had low-avidity antibodies, and 20 of 22 (90.9%) women with chronic infection displayed high-avidity antibodies. The results suggested better performance of the GRA6 avidity ELISA than the Euroimmun avidity ELISA for exclusion of a recent infection occurring less than 4 months previously. Similarly, all 35 Iranian women with acute Toxoplasma infection had low-avidity antibodies against GRA6, whereas all 34 women with chronic infection displayed IgG antibodies of high avidity, indicating the value of GRA6 avidity testing for ruling out a recent infection. Avidity tests based on lysed whole-cell Toxoplasma gondii antigen are currently used to exclude recently acquired infections; however, the use of recombinant antigen(s) might improve the diagnostic performance of avidity tests and facilitate the development of more standardized assays.

How matrix metalloproteinase (MMP)-9 (rs3918242) polymorphism affects MMP-9 serum concentration and associates with autism spectrum disorders: A case-control study in Iranian population

2021 ◽  
pp. 1-7
Author(s):  
Javid Rezaei Lord ◽  
Farhad Mashayekhi ◽  
Zivar Salehi
Keyword(s):  
Serum Concentration ◽  
Matrix Metalloproteinase ◽  
Serum Levels ◽  
Autism Spectrum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mmp9 Expression ◽  
Relationship Of ◽  
The Relationship ◽  
Metalloproteinase 9 ◽  
Control Study

Abstract The aim of this project was to evaluate the relationship of matrix metalloproteinase-9 (MMP-9) genetic variation and its serum concentration with autism spectrum disorder (ASD). One hundred ASD and 120 controls were enrolled in this study. Genomic DNA was extracted from blood and MMP-9 polymorphism was determined by polymerase chain reaction restriction fragment length polymorphism and serum levels were measured by enzyme-linked immunosorbent assay. The frequencies of CC, CT, and TT genotypes were 72%, 26%, and 2% in controls and 31%, 57%, and 12% in ASD, respectively. The frequencies of C and T alleles in ASD were 59.5% and 40.5%, and controls were 86% and 14%, respectively. There is a significant increase in serum MMP-9 levels in ASD as compared to controls. We have also shown that TT genotype is significantly associated with increase serum MMP-9 levels in patients (TT, CT, and CC serum levels were 91.77 ± 10.53, 70.66 ± 7.21, and 38.66 ± 5.52 and in controls were 55.55 ± 11.39, 42.66 ± 7.85, and 30.55 ± 6.34 ng/ml, respectively). It is concluded that there is a significant association between rs3918242 MMP-9 polymorphism and its serum concentration with autism. We also suggest that TT genotype is associated with increased MMP9 expression and may be a risk factor for ASD.

scholarly journals The Relationship of Behavior Factors with Helminthiasis Incident on Helminthiasis Infection Disease in Pregnant Mothers in Langkat Regency

2020 ◽  
Vol 2 (1) ◽  
pp. 327-342
Author(s):  
Rini Aswita ◽  
Fazidah Aguslina Siregar ◽  
Nurmaini
Keyword(s):  
Clinical Study ◽  
Pregnant Women ◽  
Sample Size ◽  
Common Type ◽  
Behavioral Factors ◽  
Study Population ◽  
Infection Disease ◽  
Relationship Of ◽  
The Relationship ◽  
Pregnant Mothers

The aim of the study is to find out the behavior factors helminthiasis Infection Disease in Pregnant Mothers. The sample size in this study was determined based on the sample size formula for testing the hypothesis of the proportion of one clinical study population of Skovland and Vatn (2007). The results of research on the influence of behavioral factors and environmental factors on helminthiasis in pregnant women in Langkat Regency in 2019 are Helminthiasis status. There are 175 pregnant women, 20 percent of pregnant women are infected with helminthiasis, and the most common type of worm is Ascaris lumbricoides. Behavioral factors. Behavioral factors have a significant influence on the incidence of helminthiasis in pregnant women, namely the habit of wearing footwear, the habit of washing hands with soap before eating and after defecation, the habit of consuming raw food and the habit of defecating in addition to toilet.

scholarly journals Relationship Of Age And Parity With Preeclampsia Incidence In Pregnant Women At dr. M. Yunus Hospital In Bengkulu

2020 ◽  
Vol 6 (2) ◽  
pp. 29-34
Author(s):  
Metha Fahriani ◽  
Sefti Fusvita Sari ◽  
Yuni Ramadhaniati
Keyword(s):  
Pregnant Women ◽  
Random Sampling ◽  
Sampling Technique ◽  
Case Control ◽  
Service Improvement ◽  
Maternal Deaths ◽  
Systematic Random Sampling ◽  
And Control ◽  
Relationship Of ◽  
The Relationship

In 2017 the number of maternal deaths was 28, consisting of 6 maternal deaths, 10 maternal deaths and 12 puerperal maternal deaths. The purpose of this study is to know the relationship of age and parity with the incidence of preeclampsia in pregnant women at dr. M. Yunus Hospital in Bengkulu year 2018. The type of research used is case control. The population in this study were all pregnant women in dr. M. Yunus Hospital in Bengkulu year 2018. The sampling technique was a case sample using total sampling, namely all pregnant women with preeclampsia and control samples using systematic random sampling technique that is sampling using multiples recorded in the hospital register of dr. M. Yunus Hospital in Bengkulu year 2018. The results of this study there is no relationship between age and the incidence of preeclampsia in pregnant women at RSUD dr. M. Yunus in 2018. With the value of p = 0.424> α = 0.05 and the value of Odds Ratio or Risk Estimate = 1.496 and there is a significant relationship between parity and the incidence of preeclampsia in pregnant women at RSUD dr. M. Yunus Bengkulu in 2018. With the value of p = 0.011 <α = 0.05 with the medium category.It is expected that the results of this study can be a program development and service improvement in reducing the prevalence of preeclampsia.

scholarly journals Kisspeptin: new biomarker of pregnancy

2019 ◽  
Vol 8 (8) ◽  
pp. 3021
Author(s):  
Priyanka Meena ◽  
Rati Mathur ◽  
Mohan L. Meena
Keyword(s):  
Pregnant Women ◽  
Gestational Age ◽  
Positive Relationship ◽  
Elisa Method ◽  
Medical College ◽  
Obstetrics And Gynaecology ◽  
Study Participants ◽  
Relationship Of ◽  
The Relationship ◽  
Single Blood Sample

Background: The recently identified hormone kisspeptin has been suggested to play an important regulatory role in placentation. The aim and objective of the study is the measurement of serum kisspeptin level in asymptomatic pregnant women and to find out the association of serum kisspeptin with gestational age in women with early pregnancy.Methods: This was a longitudinal study to the evaluation of 178 asymptomatic pregnant women with a gestation of 6 to 16 weeks attending routine antenatal booking visit recruited as study participants from the Antenatal Clinical of Obstetrics and Gynaecology Department, S.M.S. Medical College and Attached Hospitals, Jaipur, Rajasthan, India.Results: After initial clinical examination of every participant, a single blood sample was taken for the measurement of serum kisspeptin. Serum kisspeptin measurement test was performed by ELISA method and results were expressed as ng/ml. Pregnancy outcome was recorded prospectively. Mean serum kisspeptin level of study participants was 2.80±1.87ng/ml and median were 2.41 (Range 0.244-14.06ng/ml). Our result showed the relationship of serum kisspeptin with gestational age (GA) (p<0.000).Conclusions: serum kisspeptin level increases in pregnancy and showed positive relationship with gestational age significantly (p<0.000).

scholarly journals The Relationship of Nutritional Energy and Macronutrient Intake with Pregnancy Outcomes in Czech Pregnant Women

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1152
Author(s):  
Simona Najpaverova ◽  
Miroslav Kovarik ◽  
Marian Kacerovsky ◽  
Zdenek Zadak ◽  
Miloslav Hronek
Keyword(s):  
Longitudinal Study ◽  
Pregnant Women ◽  
Pregnancy Outcomes ◽  
Maternal Nutrition ◽  
Well Being ◽  
Macronutrient Intake ◽  
Dietary Records ◽  
Positive Correlations ◽  
Relationship Of ◽  
The Relationship

Maternal nutrition and metabolism play important roles for the well-being of both mother and fetus during pregnancy. This longitudinal study brings an original evaluation of the relationship between the nutritional energy and macronutrients intake (NEMI) and pregnancy outcomes and an assessment of the changes in such intake over the previous ten years. Sixty-five healthy Czech pregnant women were examined in three pregnancy periods (1st: 17th–27th; 2nd: 28th–35th; 3rd: 36th–38th gestational weeks). Results of 7-day dietary records were analyzed using NutriDan software. Energy intake decreased from 30.0 kcal/kg to 25.0 kcal/kg during pregnancy. The data also showed a decrease in macronutrients intake (p < 0.0001) with the advancing stage of pregnancy. Positive correlations were demonstrated between NEMI and birth weight (r = 0.410, p < 0.001). In the second pregnancy period, NEMI (excluding carbohydrates) positively associated with neonatal birth length (p < 0.01) and negatively with duration of birth (p < 0.05). An increased NEMI in the last period of pregnancy shortened the length of pregnancy.

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