scholarly journals Aeromonas salmonicida Growth in Response to Atlantic Salmon Mucins Differs between Epithelial Sites, Is Governed by Sialylated and N-Acetylhexosamine-Containing O-Glycans, and Is Affected by Ca2+

2017 ◽  
Vol 85 (8) ◽  
Author(s):  
János Tamás Padra ◽  
Henrik Sundh ◽  
Kristina Sundell ◽  
Vignesh Venkatakrishnan ◽  
Chunsheng Jin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atlantic Salmon ◽  
Sialic Acids ◽  
Aeromonas Salmonicida ◽  
Mucus Layer ◽  
Content Type ◽  
Salmon Aquaculture ◽  
Intestinal Mucin ◽  
Growth Promoting ◽  
Selection Of

ABSTRACT Aeromonas salmonicida causes furunculosis in salmonids and is a threat to Atlantic salmon aquaculture. The epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. By using mass spectrometry to identify mucin glycan structures with and without enzymatic removal of glycan residues, coupled to measurements of bacterial growth, we show here that the complex Atlantic salmon intestinal mucin glycans enhance A. salmonicida growth, whereas the more simple skin mucin glycans do not. Of the glycan residues present terminally on the salmon mucins, only N-acetylglucosamine (GlcNAc) enhances growth. Sialic acids, which have an abundance of 75% among terminal glycans from skin and of <50% among intestinal glycans, cannot be removed or used by A. salmonicida for growth-enhancing purposes, and they shield internal GlcNAc from utilization. A Ca2+ concentration above 0.1 mM is needed for A. salmonicida to be able to utilize mucins for growth-promoting purposes, and 10 mM further enhances both A. salmonicida growth in response to mucins and binding of the bacterium to mucins. In conclusion, GlcNAc and sialic acids are important determinants of the A. salmonicida interaction with its host at the mucosal surface. Furthermore, since the mucin glycan repertoire affects pathogen growth, the glycan repertoire may be a factor to take into account during breeding and selection of strains for aquaculture.

scholarly journals Aeromonas salmonicida Binds Differentially to Mucins Isolated from Skin and Intestinal Regions of Atlantic Salmon in anN-Acetylneuraminic Acid-Dependent Manner

2014 ◽  
Vol 82 (12) ◽  
pp. 5235-5245 ◽  
Author(s):  
János T. Padra ◽  
Henrik Sundh ◽  
Chunsheng Jin ◽  
Niclas G. Karlsson ◽  
Kristina Sundell ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Intestinal Tract ◽  
Aeromonas Salmonicida ◽  
High Morbidity ◽  
Dependent Manner ◽  
Content Type ◽  
Distal Intestine ◽  
Acetylneuraminic Acid ◽  
Host Pathogen Interaction ◽  
Glycan Chain

ABSTRACTAeromonas salmonicidasubsp.salmonicidainfection, also known as furunculosis disease, is associated with high morbidity and mortality in salmonid aquaculture. The first line of defense the pathogen encounters is the mucus layer, which is predominantly comprised of secreted mucins. Here we isolated and characterized mucins from the skin and intestinal tract of healthy Atlantic salmon and studied howA. salmonicidabound to them. The mucins from the skin, pyloric ceca, and proximal and distal intestine mainly consisted of mucins soluble in chaotropic agents. The mucin density and mucin glycan chain length from the skin were lower than were seen with mucin from the intestinal tract.A. salmonicidabound to the mucins isolated from the intestinal tract to a greater extent than to the skin mucins. The mucins from the intestinal regions had higher levels of sialylation than the skin mucins. Desialylating intestinal mucins decreasedA. salmonicidabinding, whereas desialylation of skin mucins resulted in complete loss of binding. In line with this,A. salmonicidaalso bound better to mammalian mucins with high levels of sialylation, andN-acetylneuraminic acid appeared to be the sialic acid whose presence was imperative for binding. Thus, sialylated structures are important forA. salmonicidabinding, suggesting a pivotal role for sialylation in mucosal defense. The marked differences in sialylation as well asA. salmonicidabinding between the skin and intestinal tract suggest interorgan differences in the host-pathogen interaction and in the mucin defense againstA. salmonicida.

First the seed, next the smolt?

2016 ◽  
Vol 118 (10) ◽  
pp. 2598-2612 ◽  
Author(s):  
Svein Ole Borgen ◽  
Bernt Aarset
Keyword(s):  
Atlantic Salmon ◽  
High Fecundity ◽  
Content Type ◽  
Salmon Farming ◽  
Development Path ◽  
Salmon Aquaculture ◽  
Structural Configurations ◽  
Farming Industry ◽  
Biotechnological Inventions ◽  
The Impact

Purpose The purpose of this paper is to investigate the impact of biotechnological inventions and innovations on the organization of the burgeoning Atlantic salmon farming industry. Design/methodology/approach The authors study how novel biotechnological inventions are utilized within Atlantic salmon aquaculture. The authors compare the findings with the historical development path of invention and innovation in the plant sector, and explore parallels and dissimilarities between the plant breeding sector and Atlantic salmon aquaculture. Findings The innovation capacity within Atlantic salmon aquaculture is distinct from the plant sector, but nonetheless likely to become equifinal. Similar to plants, the female fecundity of salmon is high. Hybridization, which is an effective mechanism for protection of investments in high fecundity organisms, is less effective in salmon farming because the genetic variability is higher in salmon. Hybridization provides plant breeders with significant power over grow-out farmers. The development path in Atlantic salmon sector is distinctively dissimilar from plants, but salmon farmers nonetheless appear to move toward the structural configurations that are parallel to the plant sector. The significance of new breeding technologies in Atlantic salmon farming ascends, and will play an increasingly important role in the further development of this industry. Research limitations/implications The study contributes to the prevailing knowledge of how inventions and innovations influence the future development of the Atlantic salmon industry. Originality/value Biotechnological inventions are evolving within aquaculture. So far, the implications of novel biotechnological possibilities for the Atlantic salmon sector have been underanalyzed. The paper explores these implications from the perspective of political economy.

scholarly journals Defined Deletion Mutants Demonstrate that the Major Secreted Toxins Are Not Essential for the Virulence ofAeromonas salmonicida

1998 ◽  
Vol 66 (5) ◽  
pp. 1990-1998 ◽  
Author(s):  
Richard Vipond ◽  
Ian R. Bricknell ◽  
Emma Durant ◽  
Timothy J. Bowden ◽  
Anthony E. Ellis ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Serine Protease ◽  
Extracellular Enzymes ◽  
Aeromonas Salmonicida ◽  
Deletion Mutants ◽  
Genes Encoding ◽  
Very High ◽  
Wild Type Parent ◽  
Internal Fragment ◽  
Selection Of

ABSTRACT The importance of the two major extracellular enzymes ofAeromonas salmonicida, glycerophospholipid:cholesterol acyltransferase (GCAT) and a serine protease (AspA), to the pathology and mortality of salmonid fish with furunculosis had been indicated in toxicity studies. In this study, the genes encoding GCAT (satA) and AspA (aspA) have been cloned and mutagenized by marker replacement of internal deletions, and the constructs have been used for the creation of isogenic satAand aspA mutants of A. salmonicida. A pSUP202 derivative (pSUP202sac) carrying the sacRB genes was constructed to facilitate the selection of mutants. The requirement of serine protease for processing of pro-GCAT was demonstrated. Processing involved the removal of a short internal fragment. Surprisingly, pathogenicity trials revealed no major decrease in virulence of theA. salmonicida ΔsatA::kan orA. salmonicida ΔaspA::kan mutants compared to the wild-type parent strains when Atlantic salmon (Salmo salar L.) were challenged by intraperitoneal injection. Moreover, using a cohabitation model, which more closely mimics the natural disease, there was also no significant decrease in the relative cumulative mortality following infection with either of the deletion mutants compared to the parent strain. Thus, although these two toxins may confer some competitive advantage to A. salmonicida, neither toxin is essential for the very high virulence of A. salmonicida in Atlantic salmon. This first report of defined deletion mutations within any proposed extracellular virulence factor of A. salmonicida raises crucial questions about the pathogenesis of this important fish pathogen.

PROMETHEE-based ranking of project managers based on the five personality traits

Kybernetes ◽  
2019 ◽  
Vol 49 (4) ◽  
pp. 1083-1102
Author(s):  
Georgios N. Aretoulis ◽  
Jason Papathanasiou ◽  
Fani Antoniou
Keyword(s):  
Personality Traits ◽  
Questionnaire Survey ◽  
Multicriteria Decision Making ◽  
Project Managers ◽  
Preference Ranking ◽  
Infrastructure Projects ◽  
Public Infrastructure ◽  
Content Type ◽  
The One ◽  
Selection Of

Purpose This paper aims to rank and identify the most efficient project managers (PMs) based on personality traits, using Preference Ranking Organization METHod for Enrichment Evaluations (PROMETHEE) methodology. Design/methodology/approach The proposed methodology relies on the five personality traits. These were used as the selection criteria. A questionnaire survey among 82 experienced engineers was used to estimate the required weights per personality trait. A second two-part questionnaire survey aimed at recording the PMs profile and assess the performance of personality traits per PM. PMs with the most years of experience are selected to be ranked through Visual PROMETHEE. Findings The findings suggest that a competent PM is the one that scores low on the “Neuroticism” trait and high especially on the “Conscientiousness” trait. Research limitations/implications The research applied a psychometric test specifically designed for Greek people. Furthermore, the proposed methodology is based on the personality characteristics to rank the PMs and does not consider the technical skills. Furthermore, the type of project is not considered in the process of ranking PMs. Practical implications The findings could contribute in the selection of the best PM that maximizes the project team’s performance. Social implications Improved project team communication and collaboration leading to improved project performance through better communication and collaboration. This is an additional benefit for the society, especially in the delivery of public infrastructure projects. A lot of public infrastructure projects deviate largely as far as cost and schedule is concerned and this is an additional burden for public and society. Proper project management through efficient PMs would save people’s money and time. Originality/value Identification of the best PMbased on a combination of multicriteria decision-making and psychometric tests, which focus on personality traits.

scholarly journals Glycan-protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces

2021 ◽  
Author(s):  
Yixuan Xie ◽  
Siyu Chen ◽  
Qiongyu Li ◽  
Ying Sheng ◽  
Michael R Alvarez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sialic Acid ◽  
Membrane Proteins ◽  
Cell Membranes ◽  
Sialic Acids ◽  
Cross Linking ◽  
Protein Networks ◽  
Cell Surfaces ◽  
Protein Cross Linking

A cross-linking method is developed to elucidate the glycan-mediated interactions between membrane proteins through sialic acids. The method provides previously unknown extensive glycomic interactions on cell membranes. The vast majority...

scholarly journals Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”

2021 ◽  
Author(s):  
Sergio Tomás-Martínez ◽  
Hugo B.C. Kleikamp ◽  
Thomas R. Neu ◽  
Martin Pabst ◽  
David G. Weissbrodt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pathogenic Bacteria ◽  
Extracellular Polymeric Substances ◽  
Lectin Binding ◽  
Wide Distribution ◽  
Sialic Acids ◽  
Animal Cells ◽  
Microbial Life ◽  
Proteomic Data ◽  
Accumulibacter Phosphatis

Abstract Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular matrix of virtually all microbial life and in “Candidatus Accumulibacter phosphatis”, a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs present, showing a wide distribution and variety of these carbohydrates, such as sialic acids and bacterial NulOs, in the bioaggregates. Phylogenetic analysis confirmed the potential of “Ca. Accumulibacter” to produce different types of NulOs. Proteomic analysis showed the ability of “Ca. Accumulibacter” to reutilize and reincorporate these carbohydrates. This investigation points out the importance of diverse NulOs in non-pathogenic bacteria, which are normally overlooked. Sialic acids and other NulOs should be further investigated for their role in the ecology of “Ca. Accumulibacter” in particular, and biofilms in general. Key Points •“Ca. Accumulibacter” has the potential to produce a range of nonulosonic acids. •Mass spectrometry and lectin binding can reveal the presence and location of nonulosonic acids. •The role of nonulosonic acid in non-pathogenic bacteria needs to be studied in detail.

scholarly journals Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases

Vaccines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 361
Author(s):  
Valentina Valenzuela-Muñoz ◽  
Bárbara P. Benavente ◽  
Antonio Casuso ◽  
Yeny Leal ◽  
Cristian Gallardo-Escárate
Keyword(s):  
Immune Response ◽  
Atlantic Salmon ◽  
Iron Metabolism ◽  
Iron Transport ◽  
Aeromonas Salmonicida ◽  
Transcriptional Analysis ◽  
Control Fish ◽  
Control Group ◽  
Commercial Fish ◽  
Chelating Activity

Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath®) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath® protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath® was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath® and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath® showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of iron-homeostasis-related genes in fish vaccinated with IPath® compared to the control group during the infection. Moreover, increasing expression of Atlantic salmon IgT was associated with IPath® immunization. This study provides evidence that the IPath® protein could be used as an antigen or booster in commercial fish vaccines, improving the immune response against relevant pathogens for salmon aquaculture.

scholarly journals The color change and stress response of Atlantic salmon (Salmo salar L.) infected with Aeromonas salmonicida

2021 ◽  
Vol 20 ◽  
pp. 100664
Author(s):  
Mengmeng Yi ◽  
Huanda Lu ◽  
Yishuai Du ◽  
Guoxiang Sun ◽  
Ce Shi ◽  
...  
Keyword(s):  
Stress Response ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Color Change ◽  
Aeromonas Salmonicida ◽  
Atlantic Salmon Salmo Salar

scholarly journals Cervicovaginal Microbiome Composition Is Associated with Metabolic Profiles in Healthy Pregnancy

mBio ◽  
2020 ◽  
Vol 11 (4) ◽  
Author(s):  
Andrew Oliver ◽  
Brandon LaMere ◽  
Claudia Weihe ◽  
Stephen Wandro ◽  
Karen L. Lindsay ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Reproductive Health ◽  
Microbial Communities ◽  
Carbon Sources ◽  
Amplicon Sequencing ◽  
Vaginal Microbiome ◽  
Content Type ◽  
Healthy Women ◽  
Healthy Pregnancy ◽  
Tof Ms

ABSTRACT Microbes and their metabolic products influence early-life immune and microbiome development, yet remain understudied during pregnancy. Vaginal microbial communities are typically dominated by one or a few well-adapted microbes which are able to survive in a narrow pH range and are adapted to live on host-derived carbon sources, likely sourced from glycogen and mucin present in the vaginal environment. We characterized the cervicovaginal microbiomes of 16 healthy women throughout the three trimesters of pregnancy. Additionally, we analyzed saliva and urine metabolomes using gas chromatography-time of flight mass spectrometry (GC-TOF MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) lipidomics approaches for samples from mothers and their infants through the first year of life. Amplicon sequencing revealed most women had either a simple community with one highly abundant species of Lactobacillus or a more diverse community characterized by a high abundance of Gardnerella, as has also been previously described in several independent cohorts. Integrating GC-TOF MS and lipidomics data with amplicon sequencing, we found metabolites that distinctly associate with particular communities. For example, cervicovaginal microbial communities dominated by Lactobacillus crispatus have high mannitol levels, which is unexpected given the characterization of L. crispatus as a homofermentative Lactobacillus species. It may be that fluctuations in which Lactobacillus dominate a particular vaginal microbiome are dictated by the availability of host sugars, such as fructose, which is the most likely substrate being converted to mannitol. Overall, using a multi-“omic” approach, we begin to address the genetic and molecular means by which a particular vaginal microbiome becomes vulnerable to large changes in composition. IMPORTANCE Humans have a unique vaginal microbiome compared to other mammals, characterized by low diversity and often dominated by Lactobacillus spp. Dramatic shifts in vaginal microbial communities sometimes contribute to the presence of a polymicrobial overgrowth condition called bacterial vaginosis (BV). However, many healthy women lacking BV symptoms have vaginal microbiomes dominated by microbes associated with BV, resulting in debate about the definition of a healthy vaginal microbiome. Despite substantial evidence that the reproductive health of a woman depends on the vaginal microbiota, future therapies that may improve reproductive health outcomes are stalled due to limited understanding surrounding the ecology of the vaginal microbiome. Here, we use sequencing and metabolomic techniques to show novel associations between vaginal microbes and metabolites during healthy pregnancy. We speculate these associations underlie microbiome dynamics and may contribute to a better understanding of transitions between alternative vaginal microbiome compositions.

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