scholarly journals A Novel Six-Rhodopsin System in a Single Archaeon

2010 ◽  
Vol 192 (22) ◽  
pp. 5866-5873 ◽  
Author(s):  
Hsu-Yuan Fu ◽  
Yu-Cheng Lin ◽  
Yung-Ning Chang ◽  
Hsiaochu Tseng ◽  
Ching-Che Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Global Environment ◽  
Diverse Group ◽  
Ion Transporters ◽  
Haloarcula Marismortui ◽  
Resource Limited ◽  
New Type ◽  
Ion Transportation ◽  
Proton Transporters ◽  
First Time

ABSTRACT Microbial rhodopsins, a diverse group of photoactive proteins found in Archaea, Bacteria, and Eukarya, function in photosensing and photoenergy harvesting and may have been present in the resource-limited early global environment. Four different physiological functions have been identified and characterized for nearly 5,000 retinal-binding photoreceptors, these being ion transporters that transport proton or chloride and sensory rhodopsins that mediate light-attractant and/or -repellent responses. The greatest number of rhodopsins previously observed in a single archaeon had been four. Here, we report a newly discovered six-rhodopsin system in a single archaeon, Haloarcula marismortui, which shows a more diverse absorbance spectral distribution than any previously known rhodopsin system, and, for the first time, two light-driven proton transporters that respond to the same wavelength. All six rhodopsins, the greatest number ever identified in a single archaeon, were first shown to be expressed in H. marismortui, and these were then overexpressed in Escherichia coli. The proteins were purified for absorption spectra and photocycle determination, followed by measurement of ion transportation and phototaxis. The results clearly indicate the existence of a proton transporter system with two isochromatic rhodopsins and a new type of sensory rhodopsin-like transducer in H. marismortui.

scholarly journals An Origami Paper-Based Device Printed with DNAzyme-Containing DNA Superstructures for Escherichia coli Detection

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 531 ◽  
Author(s):  
Yating Sun ◽  
Yangyang Chang ◽  
Qiang Zhang ◽  
Meng Liu
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Rolling Circle Amplification ◽  
Health And Safety ◽  
Turnaround Time ◽  
Rolling Circle ◽  
E Coli ◽  
Resource Limited ◽  
First Time ◽  
Public Health And Safety

Rapid detection of pathogenic bacteria is extremely important for public health and safety. Here, we describe for the first time an integrated origami paper-based analytical device (PAD) incorporating cell lysis, molecular recognition, amplification and visual detection of Escherichia coli (E. coli). The device features three components: paper for its ability to extract protein molecules nonspecifically from cells, DNA superstructures for their ability to immobilize RNA-cleaving DNAzymes (RCDs) but undergo target-induced RNA cleavage on paper, and isothermal rolling circle amplification (RCA) for its ability to amplify each cleavage event into repetitive sequence units that can be detected by naked eye. This device can achieve detection of E. coli K12 with a detection limit of as low as 103 CFU·mL−1 in a total turnaround time of 35 min. Furthermore, this device allowed the sensitive detection of E. coli in complex sample matrices such as juice and milk. Given that more specific RCDs can be evolved for diverse bacteria, the integrated PAD holds great potential for rapid, sensitive and highly selective detection of pathogenic bacteria in resource-limited settings.

Iso-octenidine: Promising Octenidine Analogue with Improved Solubility

2020 ◽  
Vol 17 ◽  
Author(s):  
Igor K. Yakuschenko ◽  
Nataliya N. Pozdeeva ◽  
Viktoriya A. Mumyatova ◽  
Alexey A. Terentiev ◽  
Svyatoslav Ya. Gadomsky
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Micrococcus Luteus ◽  
Octenidine Dihydrochloride ◽  
First Time

: Iso-octenidine, an isomer of octenidine dihydrochloride, was synthesized and studied for the first time. Isooctenidine was demonstrated to be 3-fold more soluble in water in comparison to original octenidine, and both substances had remarkably similar antibacterial activity (tested on Escherichia Coli and Micrococcus luteus).

A Relationship Between the Factor Indivisibility and the Output Elasticity of the Indivisible Factor

2021 ◽  
pp. 232102222098516
Author(s):  
Dipankar Das
Keyword(s):  
Production Function ◽  
Limit Point ◽  
Mathematical Proof ◽  
Wage Equation ◽  
Marginal Value ◽  
Output Elasticity ◽  
New Type ◽  
First Time ◽  
Jel Classifications

The paper puts forth a notion and derives a special type of production function where labour is an indivisible factor and is in the integer space. Thus, Newtonian calculus is not an appropriate method of deriving the marginal value because limit point does not exist. This shows that indivisibility determines the output elasticity. In the first part, the paper propounds a notion regarding how indivisibility determines curvature of the production function. In the second part, the paper incorporates the findings within a production function and derives a new type accordingly. Moreover, it formally derives the standard wage equation considering all the entitlements of labour, namely (a) normal wages, (b) interest and (c) rent of ability. So far, no such mathematical proof is there to support this wage composition. This paper, for the first time, derives this wage equation considering indivisibility of labour. JEL Classifications: J23, J24, J31, D24, C61, E24, L8

scholarly journals Structure of the master regulator Rns reveals an inhibitor of enterotoxigenic Escherichia coli virulence regulons

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Charles R. Midgett ◽  
Kacey Marie Talbot ◽  
Jessica L. Day ◽  
George P. Munson ◽  
F. Jon Kull
Keyword(s):  
Escherichia Coli ◽  
Small Molecules ◽  
Family Member ◽  
Shigella Flexneri ◽  
Decanoic Acid ◽  
Enterotoxigenic Escherichia Coli ◽  
Gram Negative Bacteria ◽  
Enteric Infections ◽  
First Time ◽  
Arac Family

AbstractEnteric infections caused by the gram-negative bacteria enterotoxigenic Escherichia coli (ETEC), Vibrio cholerae, Shigella flexneri, and Salmonella enterica are among the most common and affect billions of people each year. These bacteria control expression of virulence factors using a network of transcriptional regulators, some of which are modulated by small molecules as has been shown for ToxT, an AraC family member from V. cholerae. In ETEC the expression of many types of adhesive pili is dependent upon the AraC family member Rns. We present here the 3 Å crystal structure of Rns and show it closely resembles ToxT. Rns crystallized as a dimer via an interface similar to that observed in other dimeric AraC’s. Furthermore, the structure of Rns revealed the presence of a ligand, decanoic acid, that inhibits its activity in a manner similar to the fatty acid mediated inhibition observed for ToxT and the S. enterica homologue HilD. Together, these results support our hypothesis that fatty acids regulate virulence controlling AraC family members in a common manner across a number of enteric pathogens. Furthermore, for the first time this work identifies a small molecule capable of inhibiting the ETEC Rns regulon, providing a basis for development of therapeutics against this deadly human pathogen.

A unique environmental occurrence of hydrogen sulfide positive Escherichia coli

1980 ◽  
Vol 26 (2) ◽  
pp. 232-234
Author(s):  
Nell C. Roberts ◽  
Beverly O. Freeman ◽  
Henry B. Bradford Jr.
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Sulfide ◽  
Clinical Interest ◽  
First Time

For the first time in nearly 4 decades of surveillance, H2S positive Escherichia coli have been isolated from Calcasieu Lake and River. These results are reported because of recent clinical interest in these organisms.

scholarly journals Influence of High Pressure on the Dimerization of ToxR, a Protein Involved in Bacterial Signal Transduction

2008 ◽  
Vol 74 (24) ◽  
pp. 7821-7823 ◽  
Author(s):  
Kai Linke ◽  
Nagarajan Periasamy ◽  
Matthias Ehrmann ◽  
Roland Winter ◽  
Rudi F. Vogel
Keyword(s):  
Escherichia Coli ◽  
Signal Transduction ◽  
High Pressure ◽  
Structure And Function ◽  
Transmembrane Segments ◽  
Bacterial Signal Transduction ◽  
Reporter Strains ◽  
And Function ◽  
First Time

ABSTRACT High hydrostatic pressure (HHP) is suggested to influence the structure and function of membranes and/or integrated proteins. We demonstrate for the first time HHP-induced dimer dissociation of membrane proteins in vivo with Vibrio cholerae ToxR variants in Escherichia coli reporter strains carrying ctx::lacZ fusions. Dimerization ceased at 20 to 50 MPa depending on the nature of the transmembrane segments rather than on changes in the ToxR lipid bilayer environment.

Research on the Key Technologies for New Large Dual-Platen High-Precision Clamping System

2010 ◽  
Vol 97-101 ◽  
pp. 64-68
Author(s):  
Jian Chen ◽  
Jin Wang ◽  
Guo Dong Lu ◽  
Zheng Qi Ling
Keyword(s):  
High Precision ◽  
Large Scale ◽  
New Technologies ◽  
Impact Factors ◽  
Adaptive Correction ◽  
Hydraulic Servo ◽  
New Type ◽  
Clamping System ◽  
Plastic Parts ◽  
First Time

High- precision and large scale are the developing trend for injection molding machine clamping system .This paper compared the characteristics of three-platen toggle and dual-platen hydraulic clamping system. The key impact factors that effecting plastic parts` precision from clamping system were discussed systematically first time. Based on these analyses, a new clamping system has been proposed and manufactured to improve the plastics parts` precision, including three new technologies: new type dual-platen structure, parallelism adaptive correction technology and numerical controlled hydraulic servo system technology. It has been applied in practical machine successfully, and experiment result proves that it is effective enough to satisfying the high-precision molding of large plastics parts.

Some Origins of Nationalism in East Africa

1968 ◽  
Vol 9 (1) ◽  
pp. 119-146 ◽  
Author(s):  
J. M. Lonsdale
Keyword(s):  
Central Government ◽  
Colonial Period ◽  
Political Activity ◽  
Colonial Rule ◽  
Face To Face ◽  
National Movements ◽  
New Type ◽  
First Time ◽  
Official Recognition

This paper attempts to provide a frame of reference for evaluating the role of ordinary rural Africans in national movements, in the belief that scholarly preoccupation with élites will only partially illumine the mainsprings of nationalism. Kenya has been taken as the main field of enquiry, with contrasts and comparisons drawn from Uganda and Tanganyika. The processes of social change are discussed with a view to establishing that by the end of the colonial period one can talk of peasants rather than tribesmen in some of the more progressive areas. This change entailed a decline in the leadership functions of tribal chiefs who were also the official agents of colonial rule, but did not necessarily mean the firm establishment of a new type of rural leadership. The central part of the paper is taken up with an account of the competition between these older and newer leaderships, for official recognition rather than a mass following. A popular following was one of the conditions for such recognition, but neither really achieved this prior to 1945 except in Kikuyuland, and there the newer leaders did not want official recognition. After 1945 the newer leadership, comprising especially traders and officials of marketing co-operatives, seems everywhere to have won a properly representative position, due mainly to the enforced agrarian changes which brought the peasant face to face with the central government, perhaps for the first time. This confrontation, together with the experience of failure in earlier and more local political activity, resulted in a national revolution coalescing from below, co-ordinated rather than instigated by the educated élite.

scholarly journals The hermit сharacter as a new type of character in realistic prose by Maxim Gorky

2021 ◽  
Vol 9 (SPE2) ◽  
Author(s):  
Svetlana Strokina ◽  
Lenie Taymazova ◽  
Elvina Useinova ◽  
Ruslan Adonin
Keyword(s):  
Point Of View ◽  
Art Form ◽  
New Type ◽  
Maxim Gorky ◽  
First Time ◽  
The Church

1922-1924 was a fruitful period of Maxim Gorky's literature work. It is related to searching a new art form. The cycle “Stories of 1922-1924” is an expressive example of “new prose”. For the first time, the hermit character appeared in the cycle “Stories of 1922-1924”. From the point of view of generally recognized morality and the Church, the new type of character is ambiguous. It is characterized by both sinfulness and holiness.

scholarly journals Biogenic synthesis of silver nanoparticles from waste banana plant stems and their antibacterial activity against Escherichia coli and Staphylococcus Epidermis

2017 ◽  
Vol 5 (9) ◽  
pp. 3769 ◽  
Author(s):  
Huu Dang ◽  
Derek Fawcett ◽  
Gerrard Eddy Jai Poinern
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ag Nanoparticles ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Banana Plant ◽  
Biogenic Synthesis ◽  
Maximum Inhibition ◽  
Staphylococcus Epidermis ◽  
First Time

Background: This study for the first time presents an eco-friendly and room temperature procedure for biologically synthesizing silver (Ag) nanoparticles from waste banana plant stems.Methods: A simple and straightforward green chemistry based technique used waste banana plant stems to act as both reducing agent and capping agent to produce Ag nanoparticles, which were subsequently characterized. In addition, antibacterial studies were conducted using the Kirby-Bauer sensitivity method.Results: Advanced characterisation revealed the Ag nanoparticles had a variety of shapes including cubes, truncated triangular and hexagonal plates, and ranged in size from 70 nm up to 600 nm. The gram-negative bacteria Escherichia coli showed the maximum inhibition zone of 12 mm.Conclusions: The study has shown that waste banana plant stems can generate Ag nanoparticles with antibacterial activity against Escherichia coli and Staphylococcus epidermis.

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