Iso-octenidine: Promising Octenidine Analogue with Improved Solubility

2020 ◽  
Vol 17 ◽  
Author(s):  
Igor K. Yakuschenko ◽  
Nataliya N. Pozdeeva ◽  
Viktoriya A. Mumyatova ◽  
Alexey A. Terentiev ◽  
Svyatoslav Ya. Gadomsky
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Micrococcus Luteus ◽  
Octenidine Dihydrochloride ◽  
First Time

: Iso-octenidine, an isomer of octenidine dihydrochloride, was synthesized and studied for the first time. Isooctenidine was demonstrated to be 3-fold more soluble in water in comparison to original octenidine, and both substances had remarkably similar antibacterial activity (tested on Escherichia Coli and Micrococcus luteus).

scholarly journals Biogenic synthesis of silver nanoparticles from waste banana plant stems and their antibacterial activity against Escherichia coli and Staphylococcus Epidermis

2017 ◽  
Vol 5 (9) ◽  
pp. 3769 ◽  
Author(s):  
Huu Dang ◽  
Derek Fawcett ◽  
Gerrard Eddy Jai Poinern
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ag Nanoparticles ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Banana Plant ◽  
Biogenic Synthesis ◽  
Maximum Inhibition ◽  
Staphylococcus Epidermis ◽  
First Time

Background: This study for the first time presents an eco-friendly and room temperature procedure for biologically synthesizing silver (Ag) nanoparticles from waste banana plant stems.Methods: A simple and straightforward green chemistry based technique used waste banana plant stems to act as both reducing agent and capping agent to produce Ag nanoparticles, which were subsequently characterized. In addition, antibacterial studies were conducted using the Kirby-Bauer sensitivity method.Results: Advanced characterisation revealed the Ag nanoparticles had a variety of shapes including cubes, truncated triangular and hexagonal plates, and ranged in size from 70 nm up to 600 nm. The gram-negative bacteria Escherichia coli showed the maximum inhibition zone of 12 mm.Conclusions: The study has shown that waste banana plant stems can generate Ag nanoparticles with antibacterial activity against Escherichia coli and Staphylococcus epidermis.

scholarly journals Cloning and Heterologous Expression of Plantaricin ZJ5, a Novel Bacteriocin from Lactobacillus plantarum ZJ5, in Escherichia coli

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Dafeng Song ◽  
Ping Li ◽  
Qing Gu
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Lactobacillus Plantarum ◽  
Micrococcus Luteus ◽  
Biologically Active ◽  
Biological Method ◽  
Lac Operon ◽  
E Coli ◽  
First Time ◽  
Soluble Material

A novel bacteriocin, plantaricin ZJ5 (PZJ5) was yielded from Lactobacillus plantarum ZJ5, cloned, and produced in Escherichia coli BL21 (DE3) pLys. The PZJ5 structural gene was fused with a Trx tag, and cloned into the pET32a plasmid under the control of the inducible lac operon. Induction was performed with isopropyl-β-D-thiogalactopyranoside (IPTG), with subsequent overexpression of the fusion protein, followed by purification to homogeneity via His affinity chromatography. Recombinant E. coli produced greater quantities of PZJ5 than L. plantarum ZJ5, and PZJ5 in E. coli was expressed in the form of soluble material. Biologically active PZJ5 was recovered by cleaving the purified fusion protein using enterokinase. The released PZJ5 demonstrated antibacterial activity against Micrococcus luteus. In this study, an inexpensive biological method using a Trx fusion system was presented, and for the first time, bacteriocin PZJ5 was expressed and purified in E. coli.

scholarly journals Antibacterial Activity of Copper and Cobalt Amino Acids Complexes

2011 ◽  
Vol 39 (2) ◽  
pp. 124 ◽  
Author(s):  
ANDREEA STĂNILĂ ◽  
Cornelia BRAICU ◽  
Sorin STĂNILĂ ◽  
Raluca M. POP
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Copper Complexes ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Free Ligand ◽  
Antibacterial Properties ◽  
Moderate Activity

The antibacterial properties of differently copper and cobalt amino acids complexes on agar plates was investigated in the present study. The antibacterial activity of amino acid complexes was evaluated against on three bacteria strains (Escherichia coli, Bacillus cereus, Micrococcus luteus). Generally, the amino acids complexes were mainly active against gram-positive organisms, species like Micrococcus luteus being the most susceptible strain tested. It was registered a moderate antibacterial activity against Bacillus cereus. The microorganisms Escherichia coli, which are already known to be multi-resistant to drugs, were also resistant to the amino acids complexes but also to the free salts tested. Escherichia coli were susceptible only to the CoCl2 and copper complex with phenylalanine. The complexes with leucine and histidine seem to be more active than the parent free ligand against one or more bacterial species. Moderate activity was registered in the case of complexes with methionine and phenylalanine. From the complexes tested less efficient antibacterial activity was noted in the case of complexes with lysine and valine. These results show that cobalt and copper complexes have an antibacterial activity and suggest their potential application as antibacterial agents.

scholarly journals ZnO Nanoparticles-Chitosan Composite as Antibacterial Finish for Textiles

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Asmaa Farouk ◽  
Shaaban Moussa ◽  
Mathias Ulbricht ◽  
Torsten Textor
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Micrococcus Luteus ◽  
Sol Gel ◽  
Negative Bacterium ◽  
Hybrid Polymers ◽  
Gram Negative ◽  
Organic Hybrid ◽  
Equal Degree

The antibacterial performance of sol-gel-derived inorganic-organic hybrid polymers filled with ZnO nanoparticles-chitosan against a gram-negative bacterium Escherichia coli and a gram-positive Micrococcus luteus has been investigated. Three different molecular weights (MW) of chitosan (CTS) 1.36 · 105, 2.2 · 105, and 3.0 · 105 Da with equal degree of deacetylation (DD, 85%) (coded as S 85-60, He 85-250, and He 85-500) with equal degree of deacetylation (DD, 85%) were examined. ZnO was prepared by the base hydrolysis of zinc acetate in isopropanol using lithium hydroxide (LiOH · H2O) to hydrolyze the precursor. Sol-gel-based inorganic-organic hybrid polymers were modified with these oxides and were applied to cellulosic cotton (100%) and cotton/polyester (65/35%) fabrics. Inorganic-organic hybrids polymers were based on 3-glycidyloxypropyltrimethoxysilane (GPTMS). Bacteriological tests were performed in nutrient agar media on solid agar plates and in liquid broth systems using ZnO nanoparticles with average particle size of (40 nm). Our study showed the enhanced antibacterial activity of ZnO nanoparticles chitosan (different MW) of against a gram-negative bacterium Escherichia coli DSMZ 498 and a gram-positive Micrococcus luteus ATCC 9341 in repeated experiments. The antibacterial activity of textile treated with ZnO nanoparticles chitosan increases with decreasing the molecular weight of chitosan.

Antibacterial Ellagic Acid Derivatives and Other Constituents from Pancovia pedicellaris

2009 ◽  
Vol 64 (9) ◽  
pp. 1070-1076 ◽  
Author(s):  
René F. Soh ◽  
Jean K. Bankeu ◽  
Bruno N. Lenta ◽  
Brice M. Mbáning ◽  
Silvère Ngouela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ellagic Acid ◽  
Micrococcus Luteus ◽  
Spectroscopic Methods ◽  
Ellagic Acid Derivatives ◽  
Reported Data ◽  
Antibacterial Potential

Two new ellagic acid derivatives, named panconosides A (1) andB(2) were isolated from Pancovia pedicellaris together with eleven known compounds (3 - 13). The structures of 1 and 2, as well as those of the known compounds were established by spectroscopic methods and by comparison with previously reported data. Compounds 1 and 2 were tested in vitro for their antibacterial potential against six strains of microorganisms: Micrococcus luteus, Streptococcus ferus, Streptococcus minor, Escherichia coli, Bacillus subtilis, and Pseudomonas agarici. They were found to exhibit moderate antibacterial activity against all the tested strains compared to standard drugs.

scholarly journals Chemical and Antibacterial Polymorphism ofJuniperus oxycedrusssp.oxycedrusandJuniperus oxycedrusssp.macrocarpa(Cupressaceae) Leaf Essential Oils from Tunisia

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hnène Medini ◽  
Bruno Manongiu ◽  
Neffati Aicha ◽  
Leila Chekir-Ghedira ◽  
Fethia Harzalla-Skhiri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Juniperus Oxycedrus ◽  
Zone Diameter ◽  
Chemical Variability ◽  
Components Analysis ◽  
First Time

Essential oils fromJuniperus oxycedrusL. have been used since antiquity for fragrance, flavoring, medicinal, antimicrobial, insecticidal, and cosmetic purposes. Several works studied the chemical composition of the essential oils ofJuniperus oxycedrusleaves. The aim of this study is to investigate the chemotaxonomic relationships and antibacterial activity of two Tunisian subspecies:Juniperus oxycedrusssp.oxycedrus(L. K.) Deb. andJuniperus oxycedrusssp.macrocarpa(S. & m.) Ball. In addition, and for the first time, we reported the antibacterial activities of TunisianJ. oxycedrusssp.macrocarpaandJ. oxycedrusssp.oxycedrusagainst four bacteria. Essential oils obtained by hydrodistillation were analysed by GC and GC/MS. Fifty-five constituents were identified. Thirty four major compounds were retained for the study of the chemical variability, andα-pinene, sylvestrene,p-cymene, and 13-epi-manoyl oxide were the main ones. The chemical principal components analysis (PCA) identified three chemotypes. The study of the antibacterial activity showed thatEscherichia coliwas found to be extremely resistant (zone diameter 0 mm) to all the oils tested, whileStaphylococcus aureuswas the most sensitive strain (zone diameter 13.5 mm and MIC ranged from 600 to 650 μg/mL).

scholarly journals GC-MS analysis and antibacterial activity of some fractions from Lagochilus ilicifolius Bge. grown in Mongolia

2016 ◽  
Vol 16 ◽  
pp. 39-43
Author(s):  
M. Dumaa ◽  
Ya. Gerelt-Od ◽  
S. Javzan ◽  
D. Otgonhkishig ◽  
Ts. Doncheva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Measured Data ◽  
Gram Negative ◽  
Total Alkaloids ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Inhibition Zones ◽  
First Time ◽  
Positive Results

3-methyl-1,2,3,4-tetrahydroquinoline (1), 4-hydroxyisoquinoline (2), 4-(1E)-hydroxy-1-prophenyl)-2-methoxyphenol (3), 4-acetoxycinnamic acid (4), Songoramine (5), and Songorine (6) have been determined by GC-MS analysis from the crude alkaloid mixtures (G1) obtained from the aerial parts of Lagochilus ilicifolius Bge. grown in Mongolia and comparison of the measured data with those from the literature. The compounds 1-6 are described for the first time from L.ilicifolius. From these 3-methyl-1,2,3,4-tetrahydroquinoline (1) was determined for the first time from natural plants.In addition, the antibacterial activity of fractions and total alkaloids were evaluated against Staphylococcus aurous, Bacillus subtilis, Bacillus cereus and Escherichia coli strains, respectively. The growth inhibition zones against gram-positive S.aureus, B.subtilis, B.cereus and gram negative E.coli, strains were observed. Positive results were achieved on 500 μg/disc concentration, but lower results or no active on 100 μg/disc concentration were for the plant extracts, fractions and total alkaloids.Mongolian Journal of Chemistry 16 (42), 2015, 39-43

scholarly journals Analysis of Amoxicillin’s Antibacterial Activity Using Biological Sensor With Clit Acoustic Wave

2021 ◽  
Vol 66 (1-2) ◽  
pp. 12-18
Author(s):  
О. I. Guliy ◽  
В. D. Zaitsev ◽  
О. S. Larionova ◽  
A. M. Alsowaidi ◽  
О. A. Karavaeva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Acoustic Wave ◽  
Cell Suspension ◽  
Bottom Plate ◽  
Activity Assay ◽  
Horizontal Polarization ◽  
Express Analysis ◽  
First Time ◽  
Biological Sensor

The possibility of antibacterial activity assay using an acoustic non-contact biological sensor based on two piezoelectric plates separated by an air gap was demonstrated on the example of amoxicillin and Escherichia coli for the first time. An acoustic wave with transverse horizontal polarization is excited in the bottom plate of the sensor. The upper plate serves as the bottom of the container with the studied cell suspension. It was shown that the addition of an antibiotic to the cell suspension leads to a change in the parameters of the sensor. The effect of amoxicillin on microbial cells was monitored by laser microscopy and standard microbiological culture. The possibility of express analysis of the drug’s antibacterial activity using a biological sensor based on the use of a slit acoustic wave is shown. 

scholarly journals Comparison of Antibacterial Activity of the Spent Substrate of Pleurotus ostreatus and Lentinula edodes

2016 ◽  
Vol 8 (4) ◽  
pp. 43 ◽  
Author(s):  
A. Zepeda-Bastida ◽  
D. Ojeda-Ramírez ◽  
S. Soto-Simental ◽  
N. Rivero-Perez ◽  
M. Ayala-Martínez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pleurotus Ostreatus ◽  
Bacterial Resistance ◽  
Lentinula Edodes ◽  
Micrococcus Luteus ◽  
Inhibition Zone ◽  
Barley Straw ◽  
Bacterial Diseases

<p>Nowadays, the uncontrolled use of antibiotics has created the problem of bacterial resistance to them, what has motivated the search for new alternatives of drug for the treatment of bacterial diseases. Here, we compare antimicrobial activity of spent substrate of mushroom <em>Pleurotus ostreatus</em> and <em>Lentinula edodes</em>, against <em>Escherichia coli</em>,<em> Salmonella tiphymorium</em>,<em> Staphylococcus aureus</em> and <em>Micrococcus luteus</em>. We designed two mixtures, barley straw to be used as a substrate of cultivation of mushroom <em>Pleurotus ostreatus</em> and oats or cedar for the cultivation of mushroom <em>Lentinula edodes</em>; and were obtained aqueous extracts from spent substrates; extracts were tested for antibacterial activity. The protocol was a completely randomized assay with a factorial arrangement design. The data were analyzed with PROC GLM, SAS. The results showed that in the case of <em>Escherichia coli</em> the greatest inhibition zone was of 12.66 mm at a concentration of 6 mg mL<sup>-1</sup>, with treatment of <em>Lentinula edodes</em>/Cedar; <em>Salmonella tiphymorium</em> showed a greatest inhibition zone of 31.10 mm to a concentration of 5.12 mg mL<sup>-1</sup>, with treatment of <em>Pleurotus ostreatus</em>/Barley straw; <em>Staphylococcus aureus</em> showed a greatest inhibition zone of 9.33 mm to a concentration of 100 mg mL<sup>-1</sup>, with the treatment of <em>Lentinula edodes</em>/Cedar and finaly, <em>Micrococcus luteus </em>showed a greatest inhibition zone of 15.00 mm to a concentration of 50 mg mL<sup>-1</sup>, with the treatment <em>Lentinula edodes</em>/Oats. In conclusion, the results suggest that it is possible to use indistinctly the spent substrate of <em>Pleurotus ostreatus</em> and <em>Lentinula edodes</em> as source of extracts with antibacterial activity.</p>

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

Sign in / Sign up

Export Citation Format

Share Document