Heparan sulphate and hyaluronic acid components of the glycocalyx do not play a role in flow-mediated dilation of the iliac in the anaesthetized pig

2019 ◽  
Vol 97 (8) ◽  
pp. 746-752
Author(s):  
T. Ruane-O’Hora ◽  
D. O’Malley ◽  
M.M. Buckley ◽  
F. Markos
Keyword(s):  
Shear Stress ◽  
Hyaluronic Acid ◽  
Heparan Sulphate ◽  
Flow Mediated Dilation ◽  
Similar Increase ◽  
Shear Stress Sensor ◽  
Before And After ◽  
Student’S T ◽  
Student’S T Test

The shear-stress sensor function of vascular glycocalyx heparan sulphate and hyaluronic acid was investigated in vivo by assessing flow-mediated dilation before and after their removal. Heparinase III exposure (100 mU·mL−1 for 20 min;n = 6) did not significantly affect flow-mediated dilation of the iliac, from 0.42 ± 0.08 mm (mean ± SEM) to 0.34 ± 0.07 mm after (P = 0.12; paired Student’s t test) for a statistically similar increase in shear stress; 18.24 ± 4.2 N·m−2 for the control and 15.8 ± 3.6 N·m−2 for the heparinase III experiment (P = 0.18). Hyaluronidase exposure (0.14–1.4 mg·mL−1 for 20 min; n = 8) also did not significantly reduce flow-mediated dilation of the iliac, which averaged 0.39 ± 0.08 mm before and 0.38 ± 0.09 mm after (P = 0.11) for a statistically similar increase in shear stress; 11.90 ± 3.20 N·m−2 for the control and 9.8 ± 3.33 N·m−2 for the hyaluronidase experiment (P = 0.88). Removal of both heparan sulphate and hyaluronic acid was confirmed using immunohistochemistry. Neither the heparan sulphate nor the hyaluronic acid components of the glycocalyx mediate shear-stress-induced vasodilation in conduit arteries in vivo.

Platelets Do Not Alter Flow-Mediated Dilation or Arterial Conduction in vivo

2021 ◽  
pp. 1-6
Author(s):  
Therese Ruane-O’Hora ◽  
Farouk Markos
Keyword(s):  
Shear Stress ◽  
Platelet Count ◽  
Platelet Rich Plasma ◽  
Flow Mediated Dilation ◽  
Vascular Conductance ◽  
Arterial Blood ◽  
Vascular Bed ◽  
Before And After

The aim of this study was to investigate whether platelets contribute to shear stress and vascular conductance in the iliac vascular bed in vivo. Flow-mediated dilation of pig iliac was induced by downstream injection of acetylcholine (50 μg), and separately, conductance (ΔF/ΔP) was calculated. This was carried out before and after removal of 1 L of arterial blood in 240 mL increments, and each 240 mL was spun in a centrifuge (1,500 rcf for 7 min); platelet-rich plasma was replaced with equal volume of heparinised saline and reinjected. The circulating platelet count fell from 369 × 10<sup>9</sup>/L (<i>n</i> = 5) to 165 × 10<sup>9</sup>/L (<i>p</i> = 0.01; <i>n</i> = 4; Student’s unpaired <i>t</i>). An increase in flow led to an increase in the iliac diameter by 0.49 ± 0.03 mm (mean ± SEM) before platelet reduction and 0.55 ± 0.05 mm after (<i>p</i> = 0.36, Student’s paired <i>t</i>, <i>n</i> = 5); the change in arterial conductance was also not significantly affected by platelet reduction, control: 1.44 ± 0.34 mL/min/mm Hg, after platelet reduction: 1.39 ± 0.04 mm (<i>p</i> = 0.55, Student’s paired <i>t</i>, <i>n</i> = 4). Therefore, platelets do not contribute to shear stress or conductance in vivo.

scholarly journals Three-dimensional analysis of the root canal preparation with Reciproc Blue®, WaveOne Gold® and XP EndoShaper®: a new method in vivo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Nestor Rios-Osorio ◽  
Diana Usme ◽  
Cristian Jimenez ◽  
Adriana Pinzon ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Root Canal ◽  
Paired Comparison ◽  
Three Dimensional ◽  
New Method ◽  
Root Canal Preparation ◽  
Paired Data ◽  
Before And After ◽  
Student’S T

Abstract Background The purpose of this study was to evaluate the changes in canal volume after root canal preparation in vivo with 3 different single-file techniques (Reciproc-Blue®, WaveOne-Gold® and XP-EndoShaper®), with a new method using CBCT and 3D reconstruction. Methods In this prospective study, thirty human lower premolars from healthy patients were used, in which extraction was indicated for orthodontic reasons. All the teeth used were caries- and restoration-free with complete root development, without signs of periodontal disease or traumatic occlusion, and with only one straight canal (up to 25º curvature). Teeth were randomly divided into three different groups: Reciproc-Blue, WaveOne-Gold and XP-EndoShaper. CBCT scans before root canal preparation were used to create a 3D reconstruction with RHINOCEROS 5.0 software to assess the initial canal volume, and then compared with 3D reconstructions after canal preparation to measure the increase in canal volume. Student’s t test for paired data were used to determine statistically significant differences between the before and after canal volumes. Anova test was used to determine statistically significant differences in the percentage of canal volume increase between the groups and Tukey's post-hoc test were used to paired comparison. Results Reciproc-Blue showed the higher increase in canal volume, followed by WaveOne-Gold and XP-EndoShaper (p = 0.003). XP-EndoShaper did not show a statistically significant increase in canal volume after root canal preparation (p = 0.06). Conclusion With this model, Reciproc-Blue showed higher increase in root canal volume, followed by WaveOne-Gold, while XP-EndoShaper did not significantly increase root canal volume during preparation.

Tracings of Behavior of Myoblasts Cultured Under Couette Type of Shear Flow Between Parallel Disks

2021 ◽  
Author(s):  
Shigehiro Hashimoto ◽  
Hiroki Yonezawa
Keyword(s):  
Shear Stress ◽  
Shear Flow ◽  
Rotating Disk ◽  
Time Lapse ◽  
Mechanical Stimuli ◽  
Parallel Disks ◽  
Before And After ◽  
A Cell

Abstract A cell deforms and migrates on the scaffold under mechanical stimuli in vivo. In this study, a cell with division during shear stress stimulation has been observed in vitro. Before and after division, both migration and deformation of each cell were analyzed. To make a Couette-type shear flow, the medium was sandwiched between parallel disks (the lower stationary culture-disc and the upper rotating disk) with a constant gap. The wall shear stress (1.5 Pa &lt; τ &lt; 2 Pa) on the surface of the lower culture plate was controlled by the rotational speed of the upper disc. Myoblasts (C2C12: mouse myoblast cell line) were used in the test. After cultivation without flow for 24 hours for adhesion of the cells to the lower disk, constant τ was applied to the cells in the incubator for 7 days. The behavior of each cell during shear was tracked by time-lapse images observed by an inverted phase contrast microscope placed in the incubator. Experimental results show that each cell tends to divide after higher activities: deformation and migration. The tendency is remarkable at the shear stress of 1.5 Pa.

scholarly journals HYDROLOGICAL RESPONSES OF A WATERSHED TO VEGETATION CHANGES IN A TROPICAL SEMIARID REGION

2018 ◽  
Vol 31 (1) ◽  
pp. 161-170 ◽  
Author(s):  
EUNICE MAIA DE ANDRADE ◽  
RAFAEL DO NASCIMENTO RODRIGUES ◽  
HELBA ARAÚJO DE QUEIROZ PALÁCIO ◽  
JOSÉ BANDEIRA BRASIL ◽  
JACQUES CARVALHO RIBEIRO FILHO
Keyword(s):  
Daily Precipitation ◽  
Semiarid Region ◽  
Rainfall Characteristics ◽  
Hydrological Responses ◽  
Precipitation Water ◽  
Before And After ◽  
Student’S T ◽  
Moisture Conditions ◽  
Precipitation Events ◽  
Student’S T Test

ABSTRACT The objective of this work was to assess the effect of vegetation on the runoff coefficients of a Caatinga dry tropical forest before and after thinning. Thus, an experiment was conducted with evaluations in three hydrological years (2008, 2011 and 2013) in Iguatu, State of Ceará, Brazil. In 2008, the vegetation consisted of a 30-year regenerating Caatinga forest. The vegetation was subjected to thinning in 2009, 2011 and 2013, removing trees with less than 10-cm diameter at breast height. Hydrological responses were evaluated as a function of daily precipitation water depths, based on cumulative frequency distribution, by dividing precipitation events into three classes (CP) (CP≤30, 30<CP≤50 and CP>50 mm). Significant differences between runoff coefficients before and after vegetation thinning were assessed through the Student's t-test (p<0.01). Before thinning (2008), CP≤30 mm showed the highest runoff coefficient, differing statistically (p<0.01) from the other years. The results of precipitation events of great magnitude (CP>50 mm) indicate that the runoff is greatly dependent on rainfall characteristics and soil moisture conditions. The greater development of herbaceous vegetation due to thinning reduced the surface runoff.

Effect of in Vivo Treatment with Delta9-THC on Mice Adrenal Gland

2009 ◽  
Vol 24 (S1) ◽  
pp. 1-1
Author(s):  
E. Moura ◽  
C. Esteves-Pinto ◽  
M.P. Serrão ◽  
I. Azevedo ◽  
M. Vieira-Coelho
Keyword(s):  
Adrenal Gland ◽  
Hpa Axis ◽  
Chronic Administration ◽  
Test Results ◽  
Gland Weight ◽  
Adrenal Gland Weight ◽  
Reduce Activity ◽  
Student’S T ◽  
Student’S T Test

Introduction:The efficacy of antidepressants has been linked in part to their ability to reduce activity of the hypothalamic-pituitary-adrenal (HPA) axis; however, the mechanism by which antidepressants regulate the HPA axis is largely unknown. Recent research has demonstrated that endocannabinoids can regulate the HPA axis and exhibit antidepressant potential.Aim:The purpose of this study was therefore to evaluate the effect of chronic administration of delta-9-tetrahydrocannabinol (delta9-THC) on the adrenal gland of mice.Methods:Delta9-THC (10 mg/kg, 1 THC:1 chremophor:18 saline) or vehicle (CT, 1 chremophor:18 saline) was administered i.p. for 10 days to C57Bl6 mice aged 15 weeks. At the end of the study rats were placed in metabolic cages. Noradrenaline (NA) and adrenaline (AD) levels in samples and tissues were evaluated by HPLC-ED. Statistical analysis was done by ANOVA followed by Student's t test. Results are presented as mean±SEM.Results:Treatment with delta9-THC did not produce changes in mice weight (CT: 25±1; delta9-THC: 24±1 g, n=5-6) but produced a significant reduction in adrenal gland weight (CT: 1.4±0.2; delta9-THC: 0.6±0.1* mg, n=5-6, *P˂0.01). However, treatment with delta9-THC did not produce significant changes in NA and AD adrenal content (NA: 7.5±2.1, 5.3±0.6; AD: 14.1±1.1, 11.1±2.1 nmol, CT and delta9-THC respectively, n=5-6) or in NA and AD urine levels (NA: 0.88±0.06, 1.18±0.17; AD: 0.64±0.07, 0.81±0.09 nmol/24h, CT and delta9-THC respectively, n=5-6).Conclusion:Chronic treatment with delta9-THC reduces adrenal gland weight in mice. These results suggest that endocannabinoids may act directly at the adrenal gland to regulate the HPA axis.

scholarly journals Analysis of Effectiveness of the Social-Pedagogical Leisure Activities System for Formation of Student Youth’s Social Activity

2020 ◽  
Vol 10 (5) ◽  
pp. 24
Author(s):  
Nataliia Viacheslavivna Shepelieva ◽  
Nataliia Oleksandrivna Maksymovska ◽  
Angela Oleksandrivna Polyanichko
Keyword(s):  
Young People ◽  
Leisure Activities ◽  
Social Activity ◽  
Analysis Method ◽  
The Social ◽  
Before And After ◽  
Grouping Method ◽  
Student’S T ◽  
High Level ◽  
Student’S T Test

Formation of student youth’s social activity is the leading task of social education, especially under conditions of information society. If psychological and age traits of students as a social group are to be taken into account, the beneficial means of harmonizing this process is leisure activities. Based on interdisciplinary and integrated approaches, a system of social-pedagogical leisure activities was developed to form social activity of student youth. The aim of this article is to analyze the implementation of the social-pedagogical leisure activities system of student youth’s social activity formation. The task of this article is to study the levels of student youth’s social activity before and after implementing the social-pedagogical leisure activities system and to analyze its effectiveness using the corresponding methods. According to outlined tasks, the following methods and indices were selected and used: relative indices method, grouping method, quantitative and qualitative analysis method, Student’s t-test, graphical method. Thanks to systematic approach to experimental part of the research, it was established that the level of students’ social activity formation has improved: the specific share of young people with low level has decreased significantly, while of those with high level has increased. Group curators have noted the tendencies of young people to organize leisure activities, while students themselves were becoming involved in useful leisure practices that were new to them. Effectiveness of the new system was confirmed using statistical methods.

scholarly journals Assessment of Parkinson disease patients before and after a physical activity program

2010 ◽  
Vol 9 (1) ◽  
pp. 59-62
Author(s):  
Diego Luis Ballio Santana ◽  
Rodrigo Cruz Pinto ◽  
Alessandro Finkelsztejn ◽  
Yara Dadalti Fragoso
Keyword(s):  
Physical Activity ◽  
Parkinson Disease ◽  
Physical Activity Program ◽  
Activity Program ◽  
Special Apparatus ◽  
Before And After ◽  
Fat Composition ◽  
Student’S T ◽  
Positive Effect ◽  
Student’S T Test

OBJECTIVES: The purpose of the present study was to assess the influence of a tailored physical activity program on specific parameters in Parkinson Disease (PD) patients. METHOD: PD patients were assessed before and after six months of a tailored physical activity program. Twenty PD patients (13 M, 7 F), mean age 55 years. Aerobic, resistance and stretching exercises. No special apparatus or machine was used at any stage of the program. There was no interference with the pharmacologic treatment, which remained at the discretion of the physician in charge. Fatigue, disability, joint amplitude, cardiorespiratory parameters and body fat composition were assessed. Comparisons were performed using the Student’s t-test at baseline and after six months. RESULTS: There was a significant (p0.001) and positive effect of this physical activity program in all assessed parameters. CONCLUSIONS: Despite this chronic, disabling and progressive neurological disease, PD patients showed significant improvement in all assessed parameters after participating in a specific and tailored physical activity program.

scholarly journals 1544. Efficacy of Human-Simulated Cefiderocol Exposure Against Gram-Negative Bacteria in an Iron-Overloaded Murine Thigh Infection Model

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S563-S563
Author(s):  
James M Kidd ◽  
Kamilia Abdelraouf ◽  
David P Nicolau
Keyword(s):  
Iron Overload ◽  
Host Immunity ◽  
Infection Model ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Infection Models ◽  
Student’S T ◽  
Inoculation Treatment ◽  
Student’S T Test

Abstract Background Cefiderocol (CFDC) is a siderophore-cephalosporin conjugate which exploits bacterial iron scavenging in reaction to the hypoferremic response of host immunity and achieves potent in vivo activity against various Gram-negative bacteria (GNB). In patients with hereditary or iatrogenic hemochromatosis, the hypoferremic response may be altered by iron overload, which could hypothetically suppress the bacterial iron scavenging that bolsters CFDC efficacy. We compared CFDC efficacy between iron-overloaded (Fe+) and normal iron (NFe) murine thigh infection models. Methods Female CD-1 mice received iron dextran 100 mg/kg/d for 14 d to induce iron overload (Fe+) (ASM Microbe 2019 abstract HMB-373); an equal number of same-age mice were not dosed (NFe). On day 15, both thighs of mice rendered neutropenic were inoculated with GNB suspensions of 107 CFU/mL. Twenty CFDC-susceptible isolates with previously determined CFDC MIC from 0.25 to 4 mg/L, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriales, were used. Two hours after inoculation, treatment mice were dosed with a CFDC regimen simulating the human plasma PK profile after doses of 2g q8h (3 h infusion), while control mice were sacrificed (0 h) or dosed with saline placebo on the same schedule as the CFDC regimen (24 h). All procedures were simultaneously performed in Fe+ and NFe mice. Efficacy was defined as a change in log10 CFU/thigh at 24 h vs. 0 h and was compared between Fe+ and NFe mice for individual isolates using Student’s t-test. Results Mean (SD) bacterial burdens at 0 h in Fe+ and NFe control mice were 5.77 (0.52) and 5.76 (0.52) and log10 CFU/thigh, respectively, and, at 24 h, increased by 3.49 (0.73) and 3.42 (0.96) log10 CFU/thigh, respectively. Mean (SD) efficacies of CFDC in Fe+ and NFe mice were -1.98 (0.83) and -1.98 (0.72) log10 CFU/thigh, respectively. For 17 of 20 individual isolates, no significant differences in efficacy between Fe+ and NFe mice were observed (P > 0.05); 2 of the 3 isolates with a difference had greater efficacy in Fe+ mice. Conclusion Human-simulated exposure of CFDC is equally efficacious in iron-overloaded and normal hosts against a variety of GNB susceptible to CFDC. The potential clinical use of CFDC to treat GNB infections in patients with iron overload is supported. Disclosures All authors: No reported disclosures.

162 IMPROVED EMBRYO SURVIVAL AND QUALITY WITH EMCARE II

2006 ◽  
Vol 18 (2) ◽  
pp. 189
Author(s):  
A. Harvey ◽  
M. Lane ◽  
J. Thompson
Keyword(s):  
Calf Serum ◽  
Cell Number ◽  
Tunel Staining ◽  
Embryo Survival ◽  
Improved Outcomes ◽  
Student’S T ◽  
The Impact ◽  
Student’S T Test

Collection of embryos exposes them to a number of stresses, including light, air, and changes in temperature. Improvement of holding media to reduce the impact of handling stresses on the embryo during in vivo collection and transfer is therefore beneficial to ensure maintenance of viability following transfer. The aim of this study was to compare the effect of holding IVP-derived blastocysts at 25°C in Emcare I (ECMI, Emcare, Dallas, TX, USA) with those held in Emcare II (ECMII), a proprietry formulation designed to reduce in vitro-induced stress. In vitro-produced bovine embryos were generated using standard protocols. Blastocysts were randomly allocated to either ECMI or ECMII (ICPBio, Aukland, New Zealand) on Day 7 and were held at 25°C for a period of 24 h, after which they were cultured in Cook Bovine Blast (Cook Australia, Brisbane, Australia) supplemented with 10% fetal calf serum for 48 h. At 24 and 48 h, embryos were scored for hatching, and a cohort removed for TUNEL staining at each time point. Differences were analyzed by Student's t-test. At both 24- and 48-h culture, hatching rates tended to be higher for embryos held in ECMII than in ECMI (Table 1). The level of apoptosis at 48 h was reduced in blastocysts held in ECMII (P = 0.06). Moreover, the total cell number of hatched blastocysts at 48 h was significantly increased (1.5-fold) in those held in ECMII (P = 0.01). Results suggest that the formulation of ECMII improves the ability of IVP bovine blastocysts to re-expand and hatch following an imposed stress (25°C for 24 h). Furthermore, ECMII improves overall embryo quality through a reduction in the percentage of cells undergoing apoptosis as well as through increased cell numbers, evident 48 h following cessation of the stress. We suggest that Emcare II reduces the impact of (or increases the embryo's tolerance to and recovery from) an imposed stress, which, although severe in the present study, may provide improved outcomes following embryo transfer in field situations. Table 1. Hatching and apoptosis of blastocysts held at 25°C for 24 h in Emcare I or Emcare II This work was supported with funding by ICPBio (NZ).

132 BLASTOCYST PRODUCTION FROM BOVINE OVARIAN CORTEX FRAGMENTS XENOGRAFTED UNDER THE BACK SKIN OF MICE

2016 ◽  
Vol 28 (2) ◽  
pp. 196
Author(s):  
T. J. M. Alves ◽  
M. T. Dias ◽  
C. M. Assuncao ◽  
L. S. A. Camargo ◽  
J. H. M. Viana ◽  
...  
Keyword(s):  
The Body ◽  
Promising Alternative ◽  
Vitro Fertilization ◽  
Immunodeficient Mice ◽  
Ovarian Cortex ◽  
Standard Procedures ◽  
Before And After ◽  
Student’S T ◽  
Student’S T Test

Chemo- or radiotherapy negatively affects the fertility of female patients undergoing oncological treatments. Ovaries are sensitive to such treatments, resulting in an increasing number of premature ovarian failures. Graft techniques are a promising alternative to preserve the fertility of such patients. So far, 35 birthed from human ovarian cortex autografts were reported in the literature; however, in this approach there is a risk of neoplastic reincidence. The aim of the present study was to evaluate the feasibility of ectopic ovarian cortex xenograft (using the bovine model) under the back skin of immunodeficient mice. Female SCID mice (~60 days, n = 38) were anesthetized with ketamine/xilazine and were placed on ventral decubitus. Ovarian cortex fragments from 8 cows (1.5 mm3; n = 152) were grafted through incisions made in the dorsal region (4 grafts per mouse). Ten days after ovarian fragments xenograft, the recipients were killed and the xenografts were harvested. The mice and grafts were weighed before and after the transplant. From the xenografts recovered, 88 were either routinely processed for histology (n = 26), to evaluate the progression of folliculogenesis, or sliced (n = 62) to recover the cumulus‐oocyte complexes, which were morphologically classified and used for in vitro embryo production, using standard procedures (in vitro maturation, fertilization, and embryo culture). The remaining grafts recovered (64) were stored in liquid nitrogen for future studies. Differences between means were compared using Student’s t-test. There was no difference between the body weight of recipient mice before and after xenograft (20.5 ± 0.4 v. 21 ± 0.8 g, respectively; P > 0.05). On the other hand, the grafts increased weight (11.6 ± 3.4 v. 14.8 ± 5.2 mg before and after transplant; P < 0.01). Histological analysis of the slices showed primordial, primary, multilaminar, antral, and atretic follicles, indicating the progression of folliculogenesis and neo-angiogenesis in the grafts. Twenty-four viable cumulus‐oocyte complexes were recovered from ovarian xenografts, from which 2 blastocysts were produced in vitro 8 days later (8.3% blastocyst rate). In summary, this study showed that ovarian xenografts were (i) healthily maintained under the back skin of immunodeficient mice, (ii) responsive to murine gonadotrophins, and (iii) able to produce viable cumulus-oocyte complexes that, (iv) by in vitro fertilization, can originate blastocysts. In general, our findings show the feasibility of the ovary xenograft as an alternative technique to fertility preservation in oncogenic patients, avoiding the risk of neoplastic re-incidence. Study approved by Animal Experimentation Ethics Committee/FUSJ-009/15. Financial support was received from Fapemig and CNPq.

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