Within-family selection at an otherwise unselected locus in dairy cattle

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 433-439 ◽  
Author(s):  
L. Gomez-Raya ◽  
J. P. Gibson

Identification of allelic variants with economic importance is feasible via molecular genetic techniques. This information can be used to increase the frequency of favourable alleles in dairy cattle. The effect of selection on the genotype within families in the early stages of life is examined. Three different strategies are considered: (1) random mating of bull sires with bull dams and with cows, with embryo selection of young bulls and all cows; (2) random mating of bull sires with bull dams, with embryo selection of young bulls only; (3) minimizing or avoiding matings between homozygotes for the unfavourable allele, with embryo selection of young bulls. Selection strategies assume the use of reproductive technologies such as embryo transfer to produce large family sizes for within-family selection to be practiced. All the three strategies increase the frequency of the favourable allele rapidly. Strategy 1 gives the fastest increase in the frequency of the favourable allele. The increase in the frequency of the favourable allele is slower under random mating (strategy 2) than under a negative assortative mating (strategy 3). This is a novel example of increased selection response with negative assortative mating.Key words: selection, breeding strategies, dairy cattle.

2014 ◽  
Vol 26 (1) ◽  
pp. 12 ◽  
Author(s):  
C. Ponsart ◽  
D. Le Bourhis ◽  
H. Knijn ◽  
S. Fritz ◽  
C. Guyader-Joly ◽  
...  

Genomic tools are now available for most livestock species and are used routinely for genomic selection (GS) in cattle. One of the most important developments resulting from the introduction of genomic testing for dairy cattle is the application of reasonably priced low-density single nucleotide polymorphism technology in the selection of females. In this context, combining genome testing and reproductive biotechnologies in young heifers enables new strategies to generate replacement and elite females in a given period of time. Moreover, multiple markers have been detected in biopsies of preimplantation stage embryos, thus paving the way to develop new strategies based on preimplantation diagnosis and the genetic screening of embryos. Based on recent advances in GS, the present review focuses on new possibilities inherent in reproductive technologies used for commercial purposes and in genetic schemes, possible side effects and beneficial impacts on reproductive efficiency. A particular focus is on the different steps allowing embryo genotyping, including embryo micromanipulation, DNA production and quality assessment.


GYNECOLOGY ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 113-116
Author(s):  
L A Bagdasaryan ◽  
I E Korneyeva

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.


2016 ◽  
Vol 52 ◽  
pp. 6-12 ◽  
Author(s):  
M. V. Gladiy ◽  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holyosa ◽  
A. V. Tuchyk ◽  
...  

The main goal of dairy breeds selection should be improving breeding and productive qualities of animals under modern conditions. The majority of farms, using native breeds to produce milk, has created optimal conditions for keeping and feeding, selection and matching, growing of replacements etc. Further improvement of created native dairy breeds for economically useful traits occurs at total use of purebred Holstein bulls (semen) of foreign selection. In order to realistically assess milk productivity (milk yield, fat content in milk and fat yield) of Ukrainian Black-and-White and Red-and-White Dairy cows should be conducted a comparative analysis of Holstein cows under the same conditions of feeding and keeping. It was established that Ukrainian Red-and-White Dairy cows were characterized by the highest milk yields for 305 days of all lactations, taken into account, the among three investigated breeds. Their milk yield during the first lactation was 5933 kg of milk, during the second – 6393 kg, the third – 6391 kg and during higher lactation – 6650 kg. Ukrainian Black-and-White Dairy cows were second by milk yield (except for the second lactation), during the first lactation – 5932 kg of milk, the third – 6462 kg and higher – 6541 kg, and Holstein cows were third, during the first lactation – 5794 kg of milk, the second – 6381 kg, the third – 6335 kg and higher – 6469 kg. The fat content was almost the same and varied within 3.49-3.58% in milk of Ukrainian Red-and-White Dairy cattle, 3.50-3.60% in milk of Ukrainian Black-and-White Dairy cattle and 3.50-3.56% in Holsteins’ milk. The difference between the breeds was within 0.01-0.04%. All the investigated breeds had predominance in fat yield for three lactations over standards of these breeds: Ukrainian Red-and-White Dairy cows from 75.1 to 93.4 kg, Ukrainian Black-and-White Dairy cows – 75.1-89.0 kg respectively and Holstein cows – 41.9-60.2 kg. It was found different level of positive correlation between milk yield and fat yield in all the cases and high correlation (r = 0.604-0.921, P < 0.001) in five cases (41.7%) Negative correlation coefficients indicate that selection of animals to higher milk yield in the herd will decrease the second trait – fat content in milk. Positive and highly significant correlation between milk yield and fat yield indicates that selection of cows in the herd to higher milk yields will increase fat yield. It was revealed that bulls were among the factors impacted the milk productivity (milk yield, fat content, fat yield) of three investigated breeds. So, the force (η²x) of father’s impact on milk yield was15.4-47.9%, fat content – 22.0-43.4% and fat yield – 14.9-47.7% taking into account a lactation and a breed. The force of lines impact (η²x) was second; it was on milk yield 6.1-24.5%, fat content – 4.1-17.1 and fat yield – 5.8-23.5%. The force of breeds impact (η²x) was last; it was on milk yield 0.3-2.9%, fat content – 0.2-0.3% and fat yield – 0.6-2.7%. So, the comparative studies of milk productivity of Ukrainian Red-and-White and Black-and-White Dairy cattle with Holsteins indicate that under similar conditions of feeding and keeping, these native breeds can compete with Holstein cattle. The milk yield for 305 days of higher lactation was 6650 kg of milk in Ukrainian Red-and-White Dairy cows, 6541 kg in Ukrainian Black-and-White Dairy cows and 6469 kg in Holsteins. It was found the inverse correlation r = -0.025-0.316 between milk yield and fat content in milk in most cases. Selection and matching of animals in the herd should be carried out simultaneously on these traits. It was found positive repeatability of milk yields between the first and second, the third and higher lactations (rs = 0.036-0.741), indicating the reliability of forecasting increase in milk productivity during the next lactations in all herd. Bulls have the greatest impact (η²x) on milk productivity among the factors taken into account: milk yield – 15.4-47.9%, fat content in milk – 22.0-43.4% and fat yield – 14.9-47.7%.


Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 599
Author(s):  
Miguel A. Gutierrez-Reinoso ◽  
Pedro M. Aponte ◽  
Manuel Garcia-Herreros

Genomics comprises a set of current and valuable technologies implemented as selection tools in dairy cattle commercial breeding programs. The intensive progeny testing for production and reproductive traits based on genomic breeding values (GEBVs) has been crucial to increasing dairy cattle productivity. The knowledge of key genes and haplotypes, including their regulation mechanisms, as markers for productivity traits, may improve the strategies on the present and future for dairy cattle selection. Genome-wide association studies (GWAS) such as quantitative trait loci (QTL), single nucleotide polymorphisms (SNPs), or single-step genomic best linear unbiased prediction (ssGBLUP) methods have already been included in global dairy programs for the estimation of marker-assisted selection-derived effects. The increase in genetic progress based on genomic predicting accuracy has also contributed to the understanding of genetic effects in dairy cattle offspring. However, the crossing within inbred-lines critically increased homozygosis with accumulated negative effects of inbreeding like a decline in reproductive performance. Thus, inaccurate-biased estimations based on empirical-conventional models of dairy production systems face an increased risk of providing suboptimal results derived from errors in the selection of candidates of high genetic merit-based just on low-heritability phenotypic traits. This extends the generation intervals and increases costs due to the significant reduction of genetic gains. The remarkable progress of genomic prediction increases the accurate selection of superior candidates. The scope of the present review is to summarize and discuss the advances and challenges of genomic tools for dairy cattle selection for optimizing breeding programs and controlling negative inbreeding depression effects on productivity and consequently, achieving economic-effective advances in food production efficiency. Particular attention is given to the potential genomic selection-derived results to facilitate precision management on modern dairy farms, including an overview of novel genome editing methodologies as perspectives toward the future.


2021 ◽  
Vol 7 (1) ◽  
pp. 10-18
Author(s):  
Wilkister Nakami ◽  
◽  
Victor Tsuma ◽  

The human population is expected to double in Africa by 2030 and with it a consequent rise in demand for livestock products. Reproductive technologies improve livestock productivity however, uptake of the technologies has been low in Africa, yet this is where there is a dire need for livestock products as the human population surges. This study aimed to assess knowledge extent and understanding the use of assisted reproductive technologies in decision support for reproductive management of dairy cattle in Kenya. Dairy farmers and veterinary practitioners were interviewed using a questionnaire to obtain relevant data on knowledge, attitude and practices in the use of assisted reproductive technologies in reproductive management in dairy farms. Observable heat signs was the only method used by participants to ascertain estrus in a cow. Artificial insemination technique was adopted by all farmers as the breeding method of choice. The analysis highlighted that the respondents with low levels of formal education had less knowledge on reproductive technologies and displayed risky practices. Overall, a larger proportion of respondents indicated that the assisted reproductive technologies would be important in reproductive management. The relatively low levels of awareness, higher levels of education and a willingness to use the assisted reproductive technologies in reproductive management of dairy cattle strengthen the logic of improving productivity through the application of these assisted reproductive technologies as well as improve levels of awareness of reproductive management practices. Keywords: attitude, heat detection, knowledge, pregnancy diagnosis, practices, progesterone


2015 ◽  
Vol 43 (2) ◽  
pp. 147
Author(s):  
Roberdi , ◽  
Sobir , ◽  
Sudirman Yahya ◽  
Nurita Toruan-Mathius ◽  
Tony Liwang

<p>ABSTRACT</p><p>Molecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.</p><p>Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon</p>


Author(s):  
O. V. Gorelik ◽  
N. A. Fedoseeva ◽  
A. S. Gorelik ◽  
E. V. Koksharov

In the last few decades the improvement of domestic dairy cattle was due to the widespread use of the gene pool of the best world breed such as Holstein. As a result of crossbreeding the breeding stock of the domestic Black-and-White breed with the sires of Holstein breed in different regions of the country a population of crossbreds has been created. The purpose of the work was to study the age dynamics of the live weight of replacement heifers depending on the paternal origin and the correlation between the indicators for the growth periods. The daughters of different sires have a difference in their live weight at the first insemination. The live weight of more than 400 kg was obtained from replacement heifers obtained from the sires Saber, Decaro and Baquero. The daughters of the sires Gatedenser, Bentley and Alta Ciara in live weight were inferior to their herdmates by 2,7–9,3 kg or by 0,7–2,8 % (P ≥ 0,05). The bulk of the replacement heifers are inseminated at the age of 13 months and only the daughters of the sire Alta Ciara are inseminated at the age of 14,1 months with a live weight of 396,7 kg. Despite the almost identical live weight of heifers at birth they differed from each other in the process of rearing. It has been established that the live weight at birth is not an indicator for assessing the further growth of replacement young animals. At 6 months of age the correlation of live weight at the age of 6 months and at the first insemination was positive and ranges from 0,01 (daughters of the sire Baquero) to 0,55 (daughters of the sire Decaro). Selection of heifers at the age of 6 months by their live weight allows us to choose the best and provide the opportunity to inseminate them at the age of 12–13 months with the organization of intensive rearing.


2002 ◽  
Vol 7 (1) ◽  
Author(s):  
S. B. PINTO ◽  
V. THOMAZ-SOCCOL ◽  
R. ROCHADELLI ◽  
R. R. ANDRADE ◽  
C. R. MONTANUCCI

O presente trabalho foi desenvolvido na UFPR - Campus Palotina e em três propriedades rurais do município de Palotina, Paraná, durante o período de setembro de 1997 a agosto de 1999. O experimento foi delineado com o objetivo de estudar possíveis marcadores (parasitológicos e hematológicos) que refletem a habilidade do gado de leite (raça Holandesa Preto e Branco) em resistir à parasitose por larvas de Dermatobia hominis (Linnaeus Jr., 1781). Um total de 1050 larvas de D. hominis foram expulsas por meio de compressão digital. Os maiores valores de contagem total de leucócitos foram observados nos animais que apresentaram o menor número de nódulos de larvas de D. hominis. Study of parasitological and hematological markers for the selection of dermatobiose resistant bovines Abstract The present research work has been carried out from September 1997 through August 1999 at Federal University of Paraná Palotina Campus and in three farms from the same County. The main aim of this study was to analyze the possible parasitological and hematological markers that reflect Black and White Holstein dairy cattle resistance towards parasitism by Dermatobia hominis (Linnaeus Jr, 1781) larvae. From the group of 14 experimental animals a total of 1050 D. hominis were collected by manual compression during the period of time of the experiment. From the hematological data it was possible to conclude that there was an inverse relationship between the total leucocytes counting values and the number of larvae nodules, so that the highest the number of leucocytes per l of blood, the lowest the number of larvae nodules.


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