Plasma Cholesterol Esterification in Normal and Hyperlipoproteinemic Subjects

1973 ◽  
Vol 51 (5) ◽  
pp. 550-555 ◽  
Author(s):  
Hélène D. Fabien ◽  
Jean Davignon ◽  
Yves L. Marcel
Keyword(s):  
Total Cholesterol ◽  
Negative Correlation ◽  
Plasma Cholesterol ◽  
Relative Rate ◽  
Plasma Lipid ◽  
Normal Subjects ◽  
Cholesterol Esterification ◽  
Strong Negative Correlation ◽  
Total Group ◽  
The Mean

Plasma lipid values and the initial rates of plasma cholesterol esterification in vitro have been studied in seven normal subjects and in 34 patients with primary hyperlipoproteinemia of the different types.Compared with that of normal subjects, the mean rate of cholesterol esterification (nanomoles cholesterol esterified per hour per milliliter of plasma) is significantly increased in type II (p < 0.05), whereas the mean increases noted in types III, IV, and V are not significant. The mean relative rate (percentage cholesterol esterified per hour) is decreased in type III (p < 0.05).The correlations between plasma lipid values and cholesterol esterification have been calculated in each type as well as in normal and hyperlipoproteinemic subjects taken as a group (total group). In the total group, plasma cholesterol esterification (nanomoles per hour per milliliter) is weakly correlated with unesterified cholesterol (r = 0.384), with triglycerides (r = 0.416), and with the ratio of triglycerides to total cholesterol (r = 0.439). Only in the group of normal subjects is the rate of cholesterol esterification strongly correlated with triglycerides (r = 0.896) and with the ratio of triglycerides to total cholesterol (r = 0.885). Finally, there is a strong negative correlation (r = −0.792, p < 0.001) between the rate of cholesterol esterification expressed in the percentage of cholesterol esterified per hour and the ponderal index in the total group. This negative correlation indicates a positive correlation between cholesterol esterification and obesity. In all subjects, we have also found that negative or positive caloric balances caused the rates of cholesterol esterification to be, respectively, decreased or increased as compared with the mean of the group considered.

Testosterone precursors in spermatic venous blood of normal men and varicocele patients

1985 ◽  
Vol 108 (2) ◽  
pp. 277-283 ◽  
Author(s):  
S. Andò ◽  
C. Giacchetto ◽  
G. M. Colpi ◽  
E. Beraldi ◽  
M. L. Panno ◽  
...  
Keyword(s):  
Negative Correlation ◽  
Venous Blood ◽  
Normal Subjects ◽  
Hydroxylase Activity ◽  
Positive Correlation ◽  
Testosterone Biosynthesis ◽  
The Mean ◽  
The Individual ◽  
Normal Men

Abstract. In the present study we determined progesterone (p), 17-OH-progesterone (17-OH-P), androstenedione (Δ4), dehydroepiandrosterone (DHEA) and testosterone (T) in spermatic venous blood of 34 varicocele patients and of 13 normal subjects. We also used the DHEA/Δ4 ratio as an index of the Δ5/Δ4 pathway ratio in testosterone biosynthesis. The mean of T and Δ4 in the spermatic blood of varicocele (V) patients appeared to be significantly lower with respect to that of normal (N) subjects (T:N = 1718.2 ± 202.4 (sem) nmol/l, No. 11; V = 1243.7 ± 97 (sem) nmol/l, No. 34; P < 0.03. Δ4: N = 56.4 ± 5.6 (sem) nmol/l, No. 12; V = 38.1 ± 4 (sem) nmol/l, No. 27, 0.02> P>0.01). A negative correlation was observed between the individual age of varicocele patients and 17-OH-P (No. 34, y = −30.66x + 1300, r = −0.57, P < 0.01) Δ4 values (No. 27, y = −1.981x + 96.52, r = −0.67, P< 0.01). When the ratio of T precursors was evaluated, we observed a positive correlation between the P/17-OH-P ratio and age of varicocele (No. 33, y = 0.0065x–0.092, r = 0.45, P < 0.03). The 17-OH-P/Δ4 ratio was greatly increased with respect to that of normal subjects (N = 5.12 ± (sem), No. 12; V= 10.77 ± 1.31 (sem), No. 27; P<0.01). These data suggest that the reduced T levels in spermatic venous blood of varicocele patients were due firstly to the enzymatic deficiency of 17-20-lyase and secondly to that of 17α-hydroxylase activity as the patients grow relatively older. The negative correlation between the DHEA/Δ4 ratio and Δ4/T ratio was observed in normal subjects (No. 10, y = −0.00432x + 0.0542, r = −0.67, P < 0.03) as well as in varicocele patients (No. 27, y = −0.00399x + 0.0587, r = −0.48, 0.02 > P > 0.01). This indicates that in the testis of varicocele patients the testosterone remains prevalently supplied by the Δ5 pathway of biosynthesis.

In vitro cholesterol esterification in human serum.

1977 ◽  
Vol 23 (3) ◽  
pp. 447-453 ◽  
Author(s):  
J K Yao ◽  
P J Dyck
Keyword(s):  
Free Cholesterol ◽  
Normal Subjects ◽  
Cholesterol Esterification ◽  
Tween 20 ◽  
Fractional Rate ◽  
Significant Difference ◽  
Reproducible Method ◽  
Esterification Rate ◽  
The Mean

Abstract We report a simple, convenient, and reproducible method, involving the use of radiolabeled cholesterol dispersed in Tween 20 as a tracer and endogenous lipoproteins as a substrate, for measuring the rate of serum cholesterol esterification in vitro. The reaction of lecithin acyltransferase (EC 2.3.1.43) was enhanced by the presence of Tween 20, which probably accelerates the exchange between radiolabeled cholesterol and endogenous lipoprotein cholesterol. In sera from 65 normal subjects, the in vitro cholesterol esterification rate was significantly correlated (r=0.47,P=0.001) with age. The mean rate of esterification of 31 subjects 30 years old or younger was significantly lower than that of 34 subjects 31 to 64 years of age. We found no significant difference in the rate of esterification between men and women. The rate of cholesterol esterification (nmol/ml per h) was significantly correlated with the concentration of endogenous free cholesterol in serum, but the fractional rate (the percentage of radiolabeled cholesterol esterified per hour) was inversely proportional to the endogenous free cholesterol. The fatty acid composition of the cholesteryl esters formed by the acyltransferase reaction may provide an index in recognizing some specific disorder.

Plasma Cholesterol Levels of 6585 Children in the United States: Results of the Know Your Body Screening in Five States

PEDIATRICS ◽  
1989 ◽  
Vol 84 (6) ◽  
pp. 969-976
Author(s):  
Ken Resnicow ◽  
Jane Morley-kotchen ◽  
Ernst Wynder
Keyword(s):  
Public Health ◽  
Total Cholesterol ◽  
School Health ◽  
Plasma Cholesterol ◽  
The United States ◽  
Cholesterol Concentration ◽  
Cholesterol Levels ◽  
The Mean ◽  
Race Ethnicity ◽  
American Children

Medical and public health recommendations regarding detection and treatment of hyperlipidemia in children have generally been based on two principal epidemiologic studies: the Lipids Research Clinics Population Study (1972 to 1976) and the Bogalusa Heart Study (1973 to 1974). The present study was initiated to further describe the distribution of plasma cholesterol levels in a multiracial sample of American children. Between 1984 and 1988, the total cholesterol levels of 6585 children from 22 schools were measured as part of the Know Your Body School Health Program. For the entire population, ages 5 to 18 years, the mean total cholesterol concentration was 166.4 mg/dL. Total cholesterol was significantly greater in girls (168 mg/dL) than in boys (165 mg/dL), although sex differences were inconsistent across race/ethnicity. The mean value for blacks, 173 mg/dL, and Hispanics, 168 mg/dL, was higher than for Asians, 165 mg/dL, and whites, 163 mg/dL. Across race/ethnicity, values tended to peak between ages 8 and 10 years for girls and approximately 10 years of age for boys. These values are slightly higher than those reported in the Lipids Research Clinics and Bogalusa studies. Public health implications of these findings are discussed.

scholarly journals Randomised controlled trial of the effect of long-term selenium supplementation on plasma cholesterol in an elderly Danish population

2015 ◽  
Vol 114 (11) ◽  
pp. 1807-1818 ◽  
Author(s):  
Frederik Cold ◽  
Kristian H. Winther ◽  
Roberto Pastor-Barriuso ◽  
Margaret P. Rayman ◽  
Eliseo Guallar ◽  
...  
Keyword(s):  
Randomised Controlled Trial ◽  
Total Cholesterol ◽  
Plasma Cholesterol ◽  
Controlled Trial ◽  
Plasma Lipid ◽  
Hdl Cholesterol ◽  
Cross Sectional ◽  
Cholesterol Ratio ◽  
Randomised Controlled

AbstractAlthough cross-sectional studies have shown a positive association between Se and cholesterol concentrations, a recent randomised controlled trial in 501 elderly UK individuals of relatively low-Se status found that Se supplementation for 6 months lowered total plasma cholesterol. The Danish PRECISE (PREvention of Cancer by Intervention with Selenium) pilot study (ClinicalTrials.gov ID: NCT01819649) was a 5-year randomised, double-blinded, placebo-controlled trial with four groups (allocation ratio 1:1:1:1). Men and women aged 60–74 years (n491) were randomised to 100 (n124), 200 (n122) or 300 (n119) μg Se-enriched yeast or matching placebo-yeast tablets (n126) daily for 5 years. A total of 468 participants continued the study for 6 months and 361 participants, equally distributed across treatment groups, continued for 5 years. Plasma samples were analysed for total and HDL-cholesterol and for total Se concentrations at baseline, 6 months and 5 years. The effect of different doses of Se supplementation on plasma lipid and Se concentrations was estimated by using linear mixed models. Plasma Se concentration increased significantly and dose-dependently in the intervention groups after 6 months and 5 years. Total cholesterol decreased significantly both in the intervention groups and in the placebo group after 6 months and 5 years, with small and nonsignificant differences in changes in plasma concentration of total cholesterol, HDL-cholesterol, non-HDL-cholesterol and total:HDL-cholesterol ratio between intervention and placebo groups. The effect of long-term supplementation with Se on plasma cholesterol concentrations or its sub-fractions did not differ significantly from placebo in this elderly population.

scholarly journals Negative correlation between interleukin-2 (IL-2) serum with bacterial index in leprosy

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Patricia Dian Putri ◽  
Luh Made Mas Rusyati ◽  
Made Swastika Adiguna
Keyword(s):  
Risk Factor ◽  
Negative Correlation ◽  
Sampling Method ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Strong Negative Correlation ◽  
Cross Sectional ◽  
Leprosy Patients ◽  
The Mean ◽  
Bacterial Index

Background: Leprosy is strongly influenced by the host immunity response. One of the cytokines that play a role in cellular immunity is IL-2. IL-2 will stimulate macrophages to activate phagocytosis and form granulomas, and this occurs in the tuberculoid type of leprosy.Objective: Aim of this study was to verify the IL-2 serum levels are negatively correlated or not with bacterial index in leprosy.Methods: This study was a cross-sectional observational analytic study involving 50 leprosy subjects (41 MB and 9 PB types). Study was conducted at Dermatolovenerology Polyclinic of Sanglah General Hospital from December 2019 to March 2020. Sample was taken using a consecutive sampling method. IL-2 serum levels were measured by ELISA. Result: The mean of IL-2 serum in PB was 75.53±10.32 pg/mL and in MB was 37.59±17.13 pg/mL. Strong negative correlation (r= -0.67; p<0.001) between serum IL-2 levels with bacterial index (CI 95%= 25.96–49.93; p<0.001) was found. Every 1 pg/mL increase in IL-2 serum levels will be accompanied by a decrease in the bacterial index of 0.039 and vice versa. Determinant coefficient shows that 42.2% of bacterial index affected by the IL-2 serum levels. Low IL-2 serum levels were the risk factor of high bacterial index 2.8 times (PR= 2.8; CI 95%= 1.444–5.519; p<0.001).Conclusion: IL-2 serum levels were found to be higher in PB than MB. There was a negative correlation between IL-2 serum levels with bacterial index. Low IL-2 serum levels were the risk factor of a high bacterial index in leprosy patients.

“Ex Vivo” Influence of Fatty Acids from Plasma Cholesterol and Triglycerides on the Fatty Acid Pattern of Platelets

1985 ◽  
Vol 54 (03) ◽  
pp. 669-674 ◽  
Author(s):  
Justo Aznar ◽  
Teresa Santos ◽  
Juana Vallés
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Plasma Lipids ◽  
Ex Vivo ◽  
Plasma Cholesterol ◽  
Fatty Acid Pattern ◽  
Plasma Lipid ◽  
Normal Subjects ◽  
Lipid Fractions

SummaryWe have studied “ex vivo”, in 92 normal subjects, the influence of fatty acids (FA) that esterify plasma cholesterol and triglycerides on the fatty acid composition of phospholipids, triglycerides and free fatty acid fractions in platelets.High and significant correlations (p <0.001) were found for some of the platelet phospholipid FA and the same FA that esterify plasma cholesterol [18:11 (r = 0.56); 18:2 (r = 0.71) and 20:5 (r = 0.42)] and plasma triglycerides [18:1* (r = 0.57) and 18:2 (r = 0.66)]. Some significant correlations were also found between some of the platelet triglyceride FA and the same FA that esterify plasma phospholipids [18:1 (r = 0.58)], triglycerides [18:1 (r = 0.51), 18:2 (r = 0.52)] cholesterol [18:1 (r = 0.44)] and plasma free fatty acids [18:1 (r = 0.39); 18:2 (r = 0.40)].By evaluating these results in conjunction with those of an earlier study (1), it can be concluded that “ in vivo” the FA from different plasma lipid fractions and especially those esterifying the plasma cholesterol and phospholipid fractions, can influence the FA composition of platelet phospholipids in normal subjects. In trying to interpret the role played by plasma lipids in platelet lipids, it may be of interest to take into account the interrelationships found in this study.

scholarly journals Lipid Profile and Prevalence of Dyslipidemia in Pre- and Postmenopausal Women in Yaounde, Cameroon

2019 ◽  
pp. 1-11
Author(s):  
Nkeunen Gerard ◽  
Tsafack Takadong Julie Judith ◽  
Simo Gustave ◽  
Betrand Ayuk Tambe ◽  
Ayangma Celestin Roger ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Postmenopausal Women ◽  
Energy Homeostasis ◽  
Plasma Lipid ◽  
Military Hospital ◽  
Cross Sectional ◽  
Mean Values ◽  
Significant Difference ◽  
The Mean

Aims: Lipids serve several important physiologic functions like energy homeostasis or biosynthesis of hormone within the human body. The deterioration of lipid metabolism which is favoured by menopause is the source of cardiovascular diseases. This study aimed to determine the plasma lipid profile and dyslipidemia prevalence of some Cameroonian women. Study Design: This was a cross-sectional study. Place and Duration of Study: The enrollment took place at the Yaounde Military Hospital from November 2016 to July 2017. Methodology: To achieve this goal, two sets of 105 postmenopausal and 127 premenopausal participants were enrolled in this study. 5ml of blood were collected on EDTA coated tubes from each participant. Enzymatic methods were used to evaluate total cholesterol, HDL cholesterol and triglyceride, while LDL cholesterol was determined using the Friedewald equation. These biological parameters were used to determine dyslipidemia. Thereafter, they were compared within age and year since menopause subgroups. Results: There was no significant difference in the mean values of total cholesterol, HDL-C, LDL-C as well as triglyceride between premenopausal and postmenopausal women. The mean HDL-C concentration was stable in the age subgroups while the mean total cholesterol and LDL-C were slightly increasing as postmenopausal participants got older. LDL-C level was significantly higher in women who had been in menopause for more than 15 years. The overall dyslipidemia prevalence was 53%. Dyslipidemia was significantly higher in the subgroup of women who had freshly entered menopause. Conclusion: Even though there was no change in the mean concentration of the plasma lipid parameters, of pre- and postmenopausal participants there was a general need to improve their lifestyle in order to reduce dyslipidemia prevalence.

Relationship of Sense of Coherence to Other Psychosocial Indices

2004 ◽  
Vol 20 (4) ◽  
pp. 227-236 ◽  
Author(s):  
Sara Holmberg ◽  
Anders Thelin ◽  
Eva-Lena Stiernström
Keyword(s):  
Social Support ◽  
Psychosocial Factors ◽  
Sense Of Coherence ◽  
Living Conditions ◽  
Strong Negative Correlation ◽  
Cross Sectional ◽  
Positive Correlation ◽  
Work Related ◽  
Job Demand ◽  
The Mean

Summary: The concept of “sense of coherence” (SOC) has been widely recognized since it was first introduced by Antonovsky. The originality and usefulness of the SOC scale and its relation to other psychosocial measures has been the subject of lively debate. The aim of this paper was to test for associations between SOC and work-related psychosocial factors (mainly the Job Demand-Control model), general living conditions, education, and social network factors. Cross-sectional data from a population-based sample of 1782 rural males from nine counties in Sweden were analyzed with a multiple regression technique. The subjects were occupationally active at inclusion and the mean age was 50 years (range 40-60). SOC was assessed with the original 29-item questionnaire. Psychosocial variables and lifestyle factors were assessed using questionnaires and structured interviews. The mean SOC among the subjects was 152.3 (standard deviation, 19.4). A strong negative correlation was found between SOC and job demand, whereas a positive correlation with job control was demonstrated. A positive correlation with general living conditions and with social support was also found. However, there was no correlation to education and occupation. Thus, SOC was shown to be strongly correlated to work-related psychosocial factors and social support, but independent of sociodemographic factors.

Instant 99mTc-Labelled Glucoheptonate Kit for Kidney Imaging

1983 ◽  
Vol 22 (05) ◽  
pp. 246-250 ◽  
Author(s):  
M. Al-Hilli ◽  
H. M. A. Karim ◽  
M. H. S. Al-Hissoni ◽  
M. N. Jassim ◽  
N. H. Agha
Keyword(s):  
Sodium Hydroxide Solution ◽  
Normal Subjects ◽  
Organ Distribution ◽  
Stable Complex ◽  
Scintillation Camera ◽  
Ph Adjustment ◽  
The Mean ◽  
The Stability ◽  
Kidney Imaging ◽  
Mean Concentration

Gelchromatography column scanning has been used to study the fractions of reduced hydrolyzed 99mTc, 99mTc-pertechnetate and 99mTc-chelate in a 99mTc-glucoheptonate (GH) preparation. A stable high labelling yield of 99mTc-GH complex in the radiopharmaceutical has been obtained with a concentration of 40-50 mg of glucoheptonic acid-calcium salt and not less than 0.45 mg of SnCl2 2 H2O at an optimal pH between 6.5 and 7.0. The stability of the complex has been found significantly affected when sodium hydroxide solution was used for the pH adjustment. However, an alternative procedure for final pH adjustment of the preparation has been investigated providing a stable complex for the usual period of time prior to the injection. The organ distribution and the blood clearance data of 99mTc-GH in rabbits were relatively similar to those reported earlier. The mean concentration of the radiopharmaceutical in both kidneys has been studied in normal subjects for one hour with a scintillation camera and the results were satisfactory.

HRA Model for Hypercholesterolemia Based on a Longitudinal Health Database

1998 ◽  
Vol 37 (02) ◽  
pp. 130-133
Author(s):  
T. Kishimoto ◽  
Y. Iida ◽  
K. Yoshida ◽  
M. Miyakawa ◽  
H. Sugimori ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Proportional Hazards ◽  
Proportional Hazards Model ◽  
Model Analysis ◽  
Serum Total Cholesterol ◽  
Hazards Model ◽  
The Mean ◽  
Health Database ◽  
Observation Period

AbstractTo evaluate the risk factors for hypercholesterolemia, we examined 4,371 subjects (3,207 males and 1,164 females) who received medical checkups more than twice at an AMHTS in Tokyo during the period from 1976 through 1991; and whose serum total cholesterol was under 250 mg/dl. The mean follow-up duration was 6.6 years. A self-registering questionnaire was administered at the time of the health checkup. The endpoint of this study was the onset of hypercholesterolemia when the level of serum total cholesterol was 250 mg/dl and over. We compared two prognosis groups (normal and hypercholesterol) in terms of age, examination findings and lifestyle. After assessing each variable, we employed Cox's proportional hazards model analysis to determine the factors related to the occurrence of hypercholesterolemia. According to proportional hazards model analysis, total cholesterol, triglyceride and smoking at the beginning, and hypertension during the observation period were selected in males; and total cholesterol at the beginning and age were selected in females to determine the factors related to the occurrence of hypercholesterolemia.

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