Inhibition of Substrate-Tumor Cell Adhesion Under the Effect of Gnidilatimonoein Purified from Daphne mucronata

2004 ◽  
Vol 32 (03) ◽  
pp. 369-376 ◽  
Author(s):  
M. Mianabadi ◽  
R. Yazdanparast
Keyword(s):  
Plant Extract ◽  
Culture Medium ◽  
Membrane Glycoproteins ◽  
Active Components ◽  
Tumor Cell Adhesion ◽  
Adhesive Properties ◽  
Toxic Dose ◽  
Daphne Mucronata ◽  
Cancerous Cells ◽  
Malignancy Potential

Alteration of the cell surface glycoproteins of cancerous cells correlate with malignancy potential. To evaluate the mediation of membrane glycoproteins in wehi-164 cancerous cells, under the effect of D. mucronata crude extract and one of its purified active components, gnidilatimonoein, their attachment to fibronectin-coated wells were investigated. The plant extract, 27 μl/ml (equivalent to 0.54 mg/of plant leaves powder per ml of culture medium), as well as gnidilatimonoein (0.94 μM), were capable of quenching by 58% and 64%, respectively, the attachment of wehi-164 cells to fibronectin-coated wells (4 μg/ml). In addition to alteration of cell adhesive properties, the morphology of the treated cells were significantly changed upon treatment with the non-toxic dose of the plant extract or gnidilatimonoein. While the untreated cells have polygonal shapes, the treated cells had spherical shapes.

Production of vincristine and vinblastine by the endophytic fungus Botryosphaeria laricina strain (CRS1) is dependent on stimulating factors present in Catharanthus roseus

2020 ◽  
Vol 10 ◽  
Author(s):  
Chamara Janaka Bandara ◽  
Asitha Siriwardhana ◽  
D. Nedra Karunaratne ◽  
B. M. R. Bandara ◽  
A. Wickramasinghe ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Catharanthus Roseus ◽  
Plant Extract ◽  
Endophytic Fungus ◽  
Culture Medium ◽  
Vinca Alkaloid ◽  
Vinca Alkaloids ◽  
Alkaloid Production ◽  
Active Components ◽  
Aerial Parts

Aims: To isolate vinca alkaloid producing endophytic fungi from Catharanthus roseus and the evaluation of the factors which enhance the vincristine production. Background: An endophytic fungus Botryosphaeria laricina (CRS1) isolated from Catharanthus roseus demonstrated vinca alkaloid production under certain conditions. Method: Fungal isolates from C. roseus were grown in liquid culture and screened for alkaloid production. The strain (CRS1) producing catharanthine was sequenced and matched with GenBank. This isolated strain was studied for production of vinca alkaloids and the conditions required for vincristine and vinblastine production. Results: Eight endophytic fungi were isolated from the fresh aerial parts of C. roseus. Only CRS1, demonstrated catharanthine production. DNA sequencing of CRS1 gave a 100% match with the GenBank accession number, KC509580.1, which is related to the Botryosphaeria laricina strain JAS6. CRS1 produced only catharanthine when cultured in Czapek’s peptone liquid medium (CZ). Addition of C. roseus fresh plant extract (8.0 mL) to the culture medium (4.0 L) stimulated production of catharanthine (3.2 mg), catharanthinic acid (0.3 mg), N-demethyl-vinblastine (0.3 mg), vinblastine (2.8 mg) and vincristine (2.4 mg). However, if the added plant extract was preheated (80 ˚C, for 15 min), no vinca alkaloids appeared other than catharanthine. To identify the active fractions of the plant extract stimulating the vinca alkaloid production, the extract was dialyzed in buffer at 4 ˚C through 20 kDa MW cutoff membrane to separate into two fractions of molecules above and below 20 kDa MW .Only the fraction containing molecules above 20 kDa was able to transform catharanthine to vincristine and vinblastine. When the dialysis was performed in water instead of buffer, the larger fraction could only produce catharanthine and vinblastine. Other conditions such as the presence of light:dark (12:12 h), fructose (30.0 g L-1), glucose (30.0 g L-1), Cu2+ (0.1 mM) ions, L-tryptophan (0.1%) and succinic acid (1%) did not induce alkaloid production. Conclusions: The catharanthine producing fungal strain B. laricina (CRS1) could only produce the two vinca alkaloids vinblastine and vincristine from catharanthine in the presence of the active components larger than 20 kDa MW present in the plant extract of C. roseus,

scholarly journals Blastocyst-Bearing Sows Display a Dominant Anti-Inflammatory Cytokine Profile Compared to Cyclic Sows at Day 6 of the Cycle

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2028
Author(s):  
Inmaculada Parrilla ◽  
Cristina A. Martinez ◽  
Josep M. Cambra ◽  
Xiomara Lucas ◽  
Graça Ferreira-Dias ◽  
...  
Keyword(s):  
Culture Medium ◽  
Immune Status ◽  
Cytokine Profile ◽  
Active Components ◽  
Early Embryos ◽  
Porcine Embryo ◽  
Uterine Environment ◽  
Anti Inflammatory ◽  
Immune Tolerant ◽  
Anti Inflammatory Cytokine

In the context of porcine embryo transfer (ET) technology, understanding the tightly regulated local uterine immune environment is crucial to achieve an adequate interaction between the transferred embryos and the receiving endometrium. However, information is limited on the uterine immune status of cyclic-recipient sows when receiving embryos during ET. The present study postulated that the anti- and proinflammatory cytokine profile 6 days after the onset of estrus differs between endometria from uninseminated cyclic sows and blastocyst-bearing sows. On Day 6 of the cycle, endometrial explants were collected from sows inseminated or not inseminated during the postweaning estrus and cultured for 22 h. The culture medium was then analyzed for the contents of a total of 16 cytokines using Luminex MAP® technology. The results showed important differences in the endometrial production of most cytokines between the sow categories, with a predominant anti-inflammatory environment displayed by the blastocyst-bearing endometria. These findings suggest that sperm, seminal plasma (SP) and/or early embryos modify the uterine environment by inducing an immune-tolerant cytokine profile already visible at Day 6. Whether the SP or some of its active components may help to develop strategies to maximize the reproductive performance of recipients after ET needs further investigation.

scholarly journals Cranberry (Vaccinium Macrocarpon) Changes the surface hydrophobicity and biofilm formation of E. coli

2011 ◽  
Vol 4 ◽  
pp. MBI.S8526 ◽  
Author(s):  
Jose Uberos ◽  
Ishan Iswaldi ◽  
Rocio Rodríguez Belmonte ◽  
Antonio Segura-Carretero ◽  
Verónica Fernández-Puentes ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Urinary Infection ◽  
Haemagglutination Inhibition ◽  
Surface Hydrophobicity ◽  
Vaccinium Macrocarpon ◽  
Bacterial Suspension ◽  
Adhesive Properties ◽  
E Coli

Cranberry has been shown useful in the prevention of urinary infection by E. coli. In this study, we examined the changes in the hydrophobicity of P fimbriated E. coli and biofilm formation after incubation with commercial cranberry syrup extract at various concentrations. After incubating a bacterial suspension with cranberry at dilutions of 1:100 and 1:1000, tests of haemagglutination inhibition, surface hydrophobicity and biofilm formation were carried out. The surface hydrophobicity of E. coli decreased significantly after incubation with cranberry and this effect was not modified by the culture medium. Biofilm formation was inhibited after incubation with cranberry syrup and this effect was dependent on the culture medium. Thus, in some circumstances cranberry can modify nonspecific adhesive properties of E. coli. In previous studies, cranberry has only been implicated in the inhibition of P-fimbriated E. coli, but our observations show that it acts by modifying adhesive properties under P-related fimbriae.

scholarly journals Investigating Apoptotic Effects of Methanolic Extract of Dorema glabrum Seed on WEHI-164 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Maryam Bannazadeh Amirkhiz ◽  
Nadereh Rashtchizadeh ◽  
Hossein Nazemiyeh ◽  
Jalal Abdolalizadeh ◽  
Leila Mohammadnejad ◽  
...  
Keyword(s):  
Plant Extract ◽  
Caspase 3 ◽  
Methanolic Extract ◽  
Spherical Shape ◽  
Dependent Manner ◽  
Chromosomal Dna ◽  
Genes Expression ◽  
Apoptotic Effects ◽  
Cancerous Cells

We aimed to investigate the apoptotic effects of the methanolic extract of Dorema glabrum seed on WEHI-164, cancerous cells in comparison with L929, normal cells and compared them with the cytotoxic effects of Taxol. So, MTT test and DNA fragmentation assay were performed on cultured and treated cells. Also electrophoresis which was followed by immunoblotting was done to survey the production of Caspase-3 and Bcl2 proteins, and to inquire into their relative genes expression, RT-PCR was used. According to our findings, the methanolic extract of Dorema glabrum seed can alter cells morphology as they shrink and take a spherical shape and lose their attachment too. So, the plant extract inhibits cell growth albeit in a time- and dose-dependent manner and results in degradation of chromosomal DNA. Induction of apoptosis by the plant extract was proved by the reduction of pro-Caspase-3 and Bcl2 proteins and increase in Caspase-3 gene expression and decrease in that of bcl2 too. Our data well established the antiproliferative effect of methanolic extract of Dorema glabrum seed and clearly showed that the plant extract can induce apoptosis and not necrosis in vitro. These results demonstrated that Dorema glabrum seed might be a novel and attractive therapeutic candidate for tumor treatment.

scholarly journals The Effect of Aspirin and Ibuprofen on the Proliferation of Cervical Cancer Cells (HeLa) Compared to Non-Cancerous Cells (HEK 293) in Cell Culture Medium

2018 ◽  
Vol 12 (5) ◽  
pp. 16-24
Author(s):  
Rahim Ahmadi ◽  
Zahra Karimi Ghezeli ◽  
Foroozan Gravand ◽  
Mahin Naghshineh ◽  
◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Hek 293 ◽  
Cervical Cancer Cells ◽  
Cancerous Cells

scholarly journals 3D PRINTED HYDROGEL GLUCOSE SENSOR ON ARGON PLASMA ACTIVATED POLYSTYRENE

2020 ◽  
Vol 50 (2) ◽  
pp. 45-48
Author(s):  
Jens Jüttner ◽  
Nenad Krstic ◽  
Achim Müller ◽  
Monika Knuth ◽  
Christiane Thielemann
Keyword(s):  
Culture Medium ◽  
Glucose Sensor ◽  
Argon Plasma ◽  
High Sensitivity ◽  
Two Dimensional ◽  
Cell Culture Medium ◽  
Adhesive Properties ◽  
3D Printed ◽  
Petri Dishes ◽  
Relevant Range

This study presents a proof of principle concept for a two-dimensional bioprinted glucose sensor on Petri dishes that allows for glucose measurements in cell culture medium. To improve bioink adhesion, the polystyrene surfaces of standard Petri dishes are activated with argon plasma, which increases roughness and hydrophilicity. The bioink containing the sensor chemistry—namely fluorescently labeled ConA/Dextran embedded in alginate microbeads—was printed on the activated Petri dishes with an extrusion-based bioprinter. The printed sensor showed good stability and adhesive properties on polystyrene. The glucose concentration was examined using a standard fluorescence microscope with filters adapted to the emission wavelength of the donor and reference dyes. The printed glucose sensor showed high sensitivity and good linearity in a physiologically relevant range of glucose concentrations.

scholarly journals Hydrodynamic shear-based purification of cancer cells with enhanced tumorigenic potential

2020 ◽  
Vol 12 (1) ◽  
pp. 1-11
Author(s):  
Efraín A Cermeño ◽  
Meghan J O’Melia ◽  
Woojin M Han ◽  
Austin Veith ◽  
Graham Barber ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Adhesion Strength ◽  
Human Cancer ◽  
Low Frequency ◽  
Label Free ◽  
Tumor Initiating Cells ◽  
Adhesive Properties ◽  
Human Cancer Cells ◽  
Tumorigenic Potential ◽  
Cancerous Cells

Abstract Tumor-initiating cells (TICs), a subpopulation of cancerous cells with high tumorigenic potential and stem-cell-like properties, drive tumor progression and are resistant to conventional therapies. Identification and isolation of TICs are limited by their low frequency and lack of robust markers. Here, we characterize the heterogeneous adhesive properties of a panel of human and murine cancer cells and demonstrate differences in adhesion strength among cells, which exhibit TIC properties and those that do not. These differences in adhesion strength were exploited to rapidly (~10 min) and efficiently isolate cancerous cells with increased tumorigenic potential in a label-free manner by use of a microfluidic technology. Isolated murine and human cancer cells gave rise to larger tumors with increased growth rate and higher frequency in both immunocompetent and immunocompromised mice, respectively. This rapid and label-free TIC isolation technology has the potential to be a valuable tool for facilitating research into TIC biology and the development of more efficient diagnostics and cancer therapies.

scholarly journals Cytotoxic effects on cancerous and non-cancerous cells of trans-cinnamaldehyde, carvacrol, and eugenol

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saurav Ranjitkar ◽  
Delong Zhang ◽  
Fei Sun ◽  
Saleh Salman ◽  
Wu He ◽  
...  
Keyword(s):  
Hela Cells ◽  
Normal Skin ◽  
Antioxidant Properties ◽  
Cell Types ◽  
Active Components ◽  
Cancer Treatments ◽  
Cytotoxic Effects ◽  
Reverse Transcription Quantitative Pcr ◽  
Apoptotic Genes ◽  
Cancerous Cells

AbstractEssential oils and their active components, referred here as plant derived antimicrobials (PDAs), have been used for their antimicrobial, anti-inflammatory and antioxidant properties. Many reports also document PDAs’ cytotoxic effects on cancerous cells, raising the hope that they could be used for cancer treatments. Due to the lack of specificity, we hypothesize that PDAs are cytotoxic to both cancerous and non-cancerous cells. Trans-cinnamaldehyde (TCA), carvacrol, and eugenol were assessed for their cytotoxicity on cancerous HeLa cells and normal skin fibroblasts (CCD-1123Sk, CCD) by MTT and LDH assays, flow cytometry, and reverse transcription quantitative PCR (RT-qPCR). After 24 h of treatment, carvacrol and TCA significantly decreased cell viability (by more than 50%) at 100 µg/ml, whereas eugenol was ineffective up to 400 µg/ml. Cell detachment and significantly increased apoptosis were observed with 100 µg/ml of TCA on both cell types. RT-qPCR for apoptotic genes (BCL2, CASP3 and CASP8) and necrosis genes (MLKL, RIPK1 and RIPK3) did not show significant differences between control and treated cells of both types, with the exception of eugenol-treated HeLa cells in which expression of BCL2, MLKL and RIPK1 was significantly higher than controls. Taken together, we conclude that the three PDAs studied here exhibited similar cytotoxic effects on both cancerous and non-cancerous cells.

scholarly journals Hydroalcoholic Extracts of Eclipta Alba as a Reversal Agent in Boric Acid Induced Male Reproductive Toxicity

2012 ◽  
Vol 57 (1-4) ◽  
pp. 21-46
Author(s):  
Pooja Nair ◽  
Nidhi Vora ◽  
Kaveri Purandhar ◽  
Vishal Kamle ◽  
Sriram Seshadri
Keyword(s):  
Oxidative Stress ◽  
Boric Acid ◽  
Plant Extract ◽  
Dose Regimen ◽  
Reproductive Toxicity ◽  
Treated Group ◽  
Active Components ◽  
Reversal Agent ◽  
Eclipta Alba ◽  
Normal Ranges

AbstractBoric acid administration has a detrimental effect on reproductive organs, livers, and kidneys in rats. In the present study, male Wistar rats were orally fed with boric acid at a dose regimen of 350 mg/kg bodyweight for a period of 30 days, followed by oral administration of hydroalcoholic extract of Eclipta alba at a dose regimen of 500 mg/kg body weight orally for 60 days. The boric acid treated group showed signs of toxicity, such as liver damage, nephropathy, and complete damage to the testis, as early as 15 days which became aggravated by 30 days of the boric acid study period. Oxidative stress in all of the vital organs showed a significant increase. Following the plant extract treatment, complete reversal in reproductive toxicity was observed. The hormonal levels viz., testosterone, estrogen, and FSH returned to normal ranges after the 60 days of plant extract treatment. Significant reversal comparable to the control groups was observed in oxidative stress in the vital tissues. The fertility of the rats was found to be restored. The altered tissue morphology showed significant signs or recovery as observed under light microscopic examination. Thus, overall recovery was observed in the boric acid treated rats which received treatment with the plant extract. It can be concluded that Boric acid definitely has detrimental effects on the male reproductive system and the plant extract showed promising results. However, the mode of action and the active components for the same needs to be further investigated.

Sign in / Sign up

Export Citation Format

Share Document