Using hierarchical mesoporous Mg–Al LDH as a potential model to precisely load BSA for biological application

2021 ◽  
pp. 2050012
Author(s):  
Xiaoxuan Li ◽  
Luxin Liang ◽  
Yanni Tan ◽  
Qianli Huang ◽  
Si Yang ◽  
...  
Keyword(s):  
Sodium Dodecyl ◽  
Phosphate Buffer Solution ◽  
Bioactive Molecules ◽  
Cytotoxicity Test ◽  
Adsorption Efficiency ◽  
Buffer Solution ◽  
Electron Microscopic ◽  
Anion Surfactant ◽  
Model Drug ◽  
Double Hydroxide

Bovine serum albumin (BSA) is a familiar protein and often used as a model drug to test the adsorption efficiency of material, while the cytotoxicity of hybrid is overlooked. In this study, we synthesized hierarchical mesoporous flower-like Mg–Al layered double hydroxide (LDH) microspheres through anion surfactant mediated hydrothermal method. The scanning electron microscopic images demonstrated that 10S-LDH and 100S-LDH samples possessed a porous surface and the porosity structure were affected by the concentration of anionic surfactant, i.e., sodium dodecyl sulfonate (SDS). By being immersed in the BSA/phosphate buffer solution (PBS) solution, the 100S-LDH had a loading capacity about 44.6 mg/g. Compared to 1S-LDH and 10S-LDH, the SDS intercalated in 100S-LDH could form the hydrogen bond with BSA molecules and promote the adsorption efficiency. In the cytotoxicity test towards Raw264.7 cells, the hybrid of BSA and [Formula: see text]S-LDH showed a superior biocompatibility to the raw LDH materials. The SDS intercalated Mg–Al LDH is expected to be used in the bioactive molecules delivery.

scholarly journals Design of novel Surfactant Modified Carbon Nanotube PasteElectrochemical Sensor for the Sensitive Investigation of Tyrosineas a Pharmaceutical Drug

2019 ◽  
Vol 9 (1) ◽  
pp. 132-137 ◽  
Author(s):  
Nagarajappa Hareesha ◽  
Jamballi Gangadharappa Gowda Manjunatha ◽  
Chenthattil Raril ◽  
Girish Tigari
Keyword(s):  
Carbon Nanotube ◽  
Pharmaceutical Medicine ◽  
Sodium Dodecyl ◽  
Phosphate Buffer Solution ◽  
Differential Pulse ◽  
Electrochemical Analysis ◽  
Buffer Solution ◽  
Limit Of Quantification ◽  
Scanning Electronmicroscopy ◽  
Carbon Nanotube Paste Electrode

Purpose: The novel sodium dodecyl sulfate modified carbon nanotube paste electrode (SDS/CNTPE) was used as a sensitive sensor for the electrochemical investigation of L-tyrosine (TY).Methods: The electrochemical analysis of TY was displayed through cyclic voltammetry (CV)and differential pulse voltammetry (DPV). The surface morphology of SDS/CNTPE and barecarbon nanotube past electrode (BCNTPE) was reviewed trough field emission scanning electronmicroscopy (FESEM).Results: The functioning SDS/CNTPE shows a voltammetric response with superior sensitivitytowards TY. This study was conducted using a phosphate buffer solution having neutral pH(pH=7.0). The correlation between the oxidation peak current of TY and concentration of TYwas achieved linearly in CV method, in the range 2.0×10-6 to 5 ×10-5 M with the detection limit729 nM and limit of quantification 2.43 μM. The investigated voltammetric study at SDS/CNTPEwas also adopted in the examination of TY concentration in a pharmaceutical medicine as a realsample with the recovery of 97% to 98%.Conclusion: The modified electrode demonstrates optimum sensitivity, constancy, reproducibility,and repeatability during the electrocatalytic activity of TY.

Synthesis, characterization, and drug delivery property of 2-N-carboxymethyl-6-O-diethylaminoethyl-chitosan

e-Polymers ◽  
2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Aidi Zhang ◽  
Derun Ding ◽  
Jicun Ren ◽  
Xiangli Zhu ◽  
Youhong Yao
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Controlled Delivery ◽  
Protective Agent ◽  
Buffer Solution ◽  
Solution Ph ◽  
H Nmr ◽  
Structure Morphology ◽  
Ft Ir ◽  
Model Drug

Abstract A novel route is demonstrated for the synthesis of 2-N-carboxymethyl-6- O-diethylaminoethyl-chitosan (DEAE-CMC) by incorporation of carboxymethyl groups as hydrophilic moieties to the C2-NH2, and diethylaminoethyl groups as hydrophobic moieties to the C6-CH2OH of the structural unit of chitosan, via a protection-graft-deprotection procedure with benzaldehyde as protective agent. The structure, morphology, and thermal properties of the chitosan graft copolymers were characterized by means of FT-IR, 1H NMR, SEM, TGA and DSC. Chitosan and its derivatives were used as carrier of model drug-Vitamin B12 (VB12), and their controlled delivery behaviour in phosphate buffer solution (pH 7.4) were studied. The results show that the release rate of VB12 from the carrier of DEAE-CMC copolymer becomes much slower than that of chitosan in phosphate buffer solution.

scholarly journals Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Emrullah Beyazyıldız ◽  
Ferda Alpaslan Pınarlı ◽  
Özlem Beyazyıldız ◽  
Emine Rümeysa Hekimoğlu ◽  
Uğur Acar ◽  
...  
Keyword(s):  
Dry Eye ◽  
Secretory Granules ◽  
Phosphate Buffer Solution ◽  
Tear Film ◽  
Microscopic Analysis ◽  
Dry Eye Syndrome ◽  
Eye Drops ◽  
Buffer Solution ◽  
Electron Microscopic ◽  
Mesenchymal Stem Cell Therapy

Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats.Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n=9) or phosphate buffer solution (n=7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis.Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P<0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats.Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.

Drug Release from a pH/Temperature Sensitive Hydrogel Bead of Poly(Lysine Methyl Ester-Diacetoxy Tartaric Acid) and Sodium Alginate

2013 ◽  
Vol 711 ◽  
pp. 14-17
Author(s):  
Kui Lin Deng ◽  
Hui Juan Shen ◽  
Shu Liang Wang ◽  
Meng Wang ◽  
Xin Ma ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Drug Release ◽  
Sodium Alginate ◽  
Tartaric Acid ◽  
Phosphate Buffer Solution ◽  
Controlled Drug Release ◽  
Temperature Sensitive ◽  
Condensation Polymerization ◽  
Buffer Solution ◽  
Model Drug

In this study, a pH/temperature sensitive polymeric hydrogel from poly (lysine methyl ester-diacetoxy tartaric acid) and sodium alginate (PLMD-SA) was prepared via the condensation polymerization. Using indomethacin as the model drug, the controlled drug-release from the resulting pH/temperature sensitive PLMD-SA was evaluated as functions of pH, PLMD content and temperature by UV spectrophotometer. It was found that the release amount of indomethacin in pH=7.4 phosphate buffer solution (PBS) was evidently higher than in pH=2.3 PBS.

Immobilization of Porcine pancreatic Lipase on Vesicle-Like Silica Material with Large Pore

2012 ◽  
Vol 485 ◽  
pp. 314-317
Author(s):  
Cui Cui Wu ◽  
Guo Wei Zhou ◽  
De Lan Xu ◽  
Tian Duo Li
Keyword(s):  
Electron Microscopy ◽  
Cetyltrimethylammonium Bromide ◽  
Physical Adsorption ◽  
Sodium Dodecyl ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Excellent Thermal Stability ◽  
Silica Material ◽  
Transmission Electron ◽  
Adsorption Desorption

Vesicle-like silica (VS) was prepared by utilizing cationic surfactant cetyltrimethylammonium bromide (CTAB) and anionic surfactant sodium dodecyl sulfate (SDS) as a dual-template. The mesostructures of VS were characterized by N2adsorption-desorption isotherms, field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). Results showed that mean mesopore size of unilayer and multilayer VS was about 14 nm and shell thickness was nearly 6 nm. The VS was chosen as support for immobilization of Porcine pancreatic lipase (PPL) by physical adsorption. The immobilized PPL possessed excellent thermal stability and reusability in the hydrolysis reaction of triacetin in phosphate buffer solution.

scholarly journals Preparation and Evaluation of Doxorubicin-Loaded Micelles Based on Glycyrrhetinic Acid Modified Gelatin Conjugates for Targeting Hepatocellular Carcinoma

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Dun Fan ◽  
Jingmou Yu ◽  
Ruiqiao Yan ◽  
Xiao Xu ◽  
Yunfei Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Polymeric Micelles ◽  
Phosphate Buffer Solution ◽  
Glycyrrhetinic Acid ◽  
Buffer Solution ◽  
Human Liver Cancer ◽  
Model Drug ◽  
Preparation And Evaluation

Hepatocellular carcinoma (HCC) is one of the most prevalent fatal diseases and the incidence of HCC is increasing worldwide. Polymeric micelles with targeting groups have drawn great attention as carriers for drug delivery in HCC therapy. Herein, novel glycyrrhetinic acid modified gelatin (GA-GEL) conjugates with three substitution degrees were synthesized and characterized. Doxorubicin (DOX) was applied as a model drug. DOX-loaded GA-GEL (DOX/GA-GEL) micelles were prepared by an emulsion-solvent evaporation method. The mean diameters of DOX/GA-GEL micelles were in the range of 195–235 nm. The encapsulation efficiency of DOX/GA-GEL micelles was 63.6%–96.2%, and the loading content was 8.3%–12.5%. Drug release from DOX-loaded micelles exhibited a biphasic manner in phosphate buffer solution (PBS) at pH 7.4. DOX/GA-GEL could be efficiently accumulated into human liver cancer HepG2 cells. The IC50 values of DOX/GA-GEL-2 and DOX·HCl in HepG2 cells were 0.33 and 0.66 μg/mL, respectively. In vivo imaging analysis demonstrated that the fluorescence signals of DiR-labeled GA-GEL-2 micelles were mainly distributed in liver and H22 orthotopic tumor, indicating that GA-GEL had the liver-targeting activity. Compared to DOX·HCl, DOX/GA-GEL-2 exhibited better antitumor activity in H22 orthotopic mice. Therefore, these results indicated that GA-GEL could be used as carrier of hydrophobic drug for targeting HCC.

Release of cell-bound polygalacturonase and cellulase from mycelium of Rhizoctonia solani

1975 ◽  
Vol 21 (4) ◽  
pp. 521-526 ◽  
Author(s):  
N. Lisker ◽  
J. Katan ◽  
I. Chet ◽  
Y. Henis
Keyword(s):  
Rhizoctonia Solani ◽  
Phosphate Buffer ◽  
Mycelial Growth ◽  
Sodium Dodecyl ◽  
Phosphate Buffer Solution ◽  
Tween 80 ◽  
Buffer Solution ◽  
Sorbitan Monooleate ◽  
Osmotic Effect ◽  
Triton X

Propagules of Rhizoctonia solani grown in modified Czapek's medium containing sodium polypectate or carboxymethyl cellulose as a sole carbon source produced both extracellular and cell-bound polygalacturonase (PG), and cellulase (Cx), respectively. The cell-bound enzymes can be released to various extents by shaking the germinating propagules in solutions of NaCl, KCl, phosphate buffer, Na2EDTA (ethylenediaminetetraacetate), detergents such as Triton X-100 (octyl phenoxypolyethoxyethanol), Tween 80 (polyoxyethylene sorbitan monooleate), Celmusol, and distilled water. Sodium dodecyl sulfate (SDS) inactivated both PG and Cx but did not affect Cx activity in phosphate buffer solution. PG was more easily released by salts from the mycelium of R. solani than Cx. The release of both enzymes was a passive process and was not due to an osmotic effect. The amount of the cell-bound fraction was correlated with the total amount of the extracellular fraction rather than with the mycelial growth. At least one-third of the cell-bound fractions of both enzymes was found to be associated with the cell wall fraction of the mycelium.

The three-dimensional fine structure of the human heart: a scanning electron microscopic atlas for research and education

Biologia ◽  
2017 ◽  
Vol 72 (12) ◽  
Author(s):  
Paulína Gálfiová ◽  
Štefan Polák ◽  
Renáta Mikušová ◽  
Andrea Gažová ◽  
Daniel Kosnáč ◽  
...  
Keyword(s):  
Fine Structure ◽  
Human Heart ◽  
Three Dimensional ◽  
Phosphate Buffer Solution ◽  
Heart Tissue ◽  
The Body ◽  
Buffer Solution ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Research And Education

AbstractKnowledge about the three-dimensional fine structure of human heart, as a crucial vital organ of the body, is not only fascinating from the scientific or educational points of view, but has a very important clinical impact. Therefore, we decided to create a three-dimensional atlas of fine structure of the human heart. Tissue samples from ten human hearts were rinsed in phosphate-buffer solution, fixed by glutaraldehyde buffered solution, and post-fixed in osmium tetroxide solution. A gentle dehydration with ethanol in different concentration and drying at the critical point of CO

Electrochemical Study of a Nano Vesicular Artificial Peroxidase on a Functional Nano Complex Modified Glassy Carbon Electrode

2013 ◽  
Vol 16 (2) ◽  
pp. 089-095 ◽  
Author(s):  
Wei-Yun Yang ◽  
Jun Hong ◽  
Ying-Xue Zhao ◽  
Bao-Lin Xiao ◽  
Yun-Fei Gao ◽  
...  
Keyword(s):  
Modified Electrode ◽  
Glassy Carbon ◽  
Sodium Dodecyl ◽  
Phosphate Buffer Solution ◽  
Nano Particles ◽  
Buffer Solution ◽  
Transfer Constant ◽  
Nano Structure ◽  
Heterogeneous Electron Transfer ◽  
Carbon Nano Tubes

A nano vesicular was constructed with the complex consisting of hemin, imidazole Gemini and sodium dodecyl sulfate (SDS) in 50 mM phosphate buffer solution (PBS). The novel nano structure acted as an efficient artificial peroxidase (AP). The AP was also immobilized on a carboxyl functionalized multi-walled carbon nano-tubes (MWCNTs-COOH) and gold nano-particles (AuNPs) nano-complex modified glassy carbon (GC) electrode, with a formal potential of -31 ± 2 mV vs. Ag/AgCl in 50 mM PBS, at a scan rate of 0.05 Vs -1. The heterogeneous electron transfer constant (ks) was evaluated to be 5.4 ± 0.1s-1. The modified electrode can be used to detect hydrogen peroxide in the range from 0.03 to 160 μM linearly, with a detection limit of 0.03 μM. The apparent Michaelis-Menten constant (Kmapp) of AP modified electrode was evaluated to be 0.034 ± 0.003mM.

A pH-Sensitive Poly (2-(Acryloyloxy) Propanoic Acid) Hydrogel and its Drug Release Behaviors

2012 ◽  
Vol 455-456 ◽  
pp. 901-906
Author(s):  
Kui Lin Deng ◽  
Chun Yuan Huang ◽  
Xiao Bo Ren ◽  
Yu Bo Gou ◽  
Hai Bin Zhong ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Salicylic Acid ◽  
Drug Release ◽  
Phosphate Buffer Solution ◽  
Ph Sensitive ◽  
Propanoic Acid ◽  
Buffer Solution ◽  
H Nmr ◽  
Model Drug

A novel pH-sensitive poly (2-(acryloyloxy) propanoic acid) hydrogel (PAPA) synthesized from lactic acid by radical polymerization has been investigated in this work. The structure of prepared monomer2-(acryloyloxy) propanoic acid) (APA) has been characterized by FTIR and1H-NMR measurements. PAPA hydrogel demonstrated an obvious pH sensitivity in its swelling in the range of 4-7 pH of phosphate buffer solution (PBS). The rapid response of the hydrogel makes it suitable for drug delivery application. Here, salicylic acid (SA) selected as a model drug, thein-vitrodrug release as a function of pH and temperature was studied systematically. The cumulative release of salicylic acid reached up to 86.6% in pH=7.4 PBS at 37.0°C within 550min. In pH=2.1 PBS, however, only 26.6% drug was released from the pH-sensitive PAPA on the same conditions.

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