scholarly journals Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes

2006 ◽  
Vol 291 (2) ◽  
pp. E372-E380 ◽  
Author(s):  
Delphine Gardan ◽  
Florence Gondret ◽  
Isabelle Louveau
Keyword(s):  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Trapezius Muscle ◽  
Growing Pigs ◽  
Hormone Sensitive Lipase ◽  
Muscle Physiology ◽  
Mrna Levels ◽  
Secretory Function ◽  
Gene Expressions ◽  
Age Related

The function of adipocytes interspersed between myofiber fasciculi in skeletal muscle physiology and physiopathology is poorly documented. Because regional differences in adipocyte features have been reported in various species, we hypothesized that lipid metabolism and secretory function of intramuscular (IM) adipocytes differ from that of nonmuscular adipocytes. In the present study, adipocytes isolated from trapezius muscle were compared with subcutaneous and perirenal adipocytes in growing pigs. Between 80 and 210 days of age, gene expressions and/or activities of enzymes involved in lipogenesis or lipolysis were much lower ( P < 0.05) in adipocytes isolated from muscle than in those from other locations. Insulin-induced lipogenesis and lipolytic efficiency after catecholamine addition were also the lowest ( P < 0.05) in IM adipocytes. In these cells, the age-related increase (+300%) in the ratio of mRNA levels of fatty acid synthase to hormone-sensitive lipase paralleled the enlargement of adipocyte diameters (+70%, P < 0.05) and the increase in lipid content in muscle (+135%, P < 0.05) during growth. Expressions of genes coding for leptin, adiponectin, and IGF-I, as well as for various hormonal receptors, were lower ( P < 0.05) in IM adipocytes than in other adipocytes, whereas levels of TNF-α mRNA did not differ between sites. Interestingly, IGF-II mRNA levels were higher ( P < 0.05) in IM adipocytes than in other adipocytes. These data support the view that IM fat is not just an ectopic extension of other fat locations but displays specific biological features during growth.

scholarly journals Dietary zinc addition influenced zinc and lipid deposition in the fore- and mid-intestine of juvenile yellow catfishPelteobagrus fulvidraco

2017 ◽  
Vol 118 (8) ◽  
pp. 570-579 ◽  
Author(s):  
Guang-Hui Chen ◽  
Christer Hogstrand ◽  
Zhi Luo ◽  
Dian-Guang Zhang ◽  
Shi-Cheng Ling ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
Pelteobagrus Fulvidraco ◽  
Lipid Deposition ◽  
Mrna Levels ◽  
Yellow Catfish ◽  
Element Binding Protein ◽  
Zinc Addition ◽  
Glucose 6 Phosphate Dehydrogenase

AbstractThe present study explored the mechanisms of dietary Zn influencing Zn and lipid deposition in the fore- and mid- intestine in yellow catfishPelteobagrus fulvidraco, and investigated whether the mechanism was intestinal-region dependent. For this purpose, yellow catfish were fed three diets containing Zn levels of 8·83, 19·20 and 146·65 mg Zn/kg, respectively. Growth performance, intestinal TAG and Zn contents as well as activities and mRNA expression of enzymes and genes involved in Zn transport and lipid metabolism in the fore- and mid-intestine were analysed. Dietary Zn increased Zn accumulation as well as activities of Cu-, Zn-superoxide dismutase and ATPase in the fore- and mid-intestine. In the fore-intestine, dietary Zn up-regulated mRNA levels of ZnT1, ZnT5, ZnT7, metallothionein (MT) and metal response element-binding transcription factor-1 (MTF-1), but down-regulated mRNA levels of ZIP4 and ZIP5. In the mid-intestine, dietary Zn up-regulated mRNA levels of ZnT1, ZnT5, ZnT7, MT and MTF-1, but down-regulated mRNA levels of ZIP4 and ZIP5. Dietary Zn reduced TAG content, down-regulated activities of 6-phosphogluconate dehydrogenase (6PGD), glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME) and fatty acid synthase (FAS) activities, and reduced mRNA levels of 6PGD, G6PD, FAS, PPARγand sterol-regulator element-binding protein (SREBP-1), but up-regulated mRNA levels of carnitine palmitoyltransferase IA, hormone-sensitive lipase (HSLa), adipose TAG lipase (ATGL) and PPARαin the fore-intestine. In the mid-intestine, dietary Zn reduced TAG content, activities of G6PD, ME, isocitrate dehydrogenase and FAS, down-regulated mRNA levels of 6PGD, G6PD, FAS, acetyl-CoA carboxylase a, PPARγand SREBP-1, but up-regulated mRNA expression of HSLa, ATGL and PPARγ. The reduction in TAG content following Zn addition was attributable to reduced lipogenesis and increased lipolysis, and similar regulatory mechanisms were observed between the fore- and mid-intestine.

scholarly journals Piceatannol, a Natural Stilbene, Extends the Lifespan of Caenorhabditis elegans Under Fasting Through Inhibition of Lipolysis

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 38-38
Author(s):  
Jang Miran ◽  
Zhang Yuan ◽  
Bai Juan ◽  
Jun-Bae An ◽  
Park Yeonhwa ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Caenorhabditis Elegans ◽  
Negative Energy ◽  
Hormone Sensitive Lipase ◽  
Pcr Analysis ◽  
Lifespan Extension ◽  
Mrna Levels ◽  
Free Glycerol ◽  
C Elegans ◽  
Glycerol Level

Abstract Objectives Lipolysis is the catabolic process that hydrolyzes triglyceride (TG) to free fatty acids (FFAs) and glycerol under negative energy balance such as fasting. In adipocytes, adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase play key roles in a series of TG hydrolysis reactions in mammals. However, overly activated adipose lipolysis is believed to contribute to link between obesity and systemic inflammation and oxidative stress. We previously demonstrated that piceatannol (PIC), a natural resveratrol analogue, inhibits adipogenesis in cultured adipocytes and lipogenesis in Caenorhabditis elegans. Furthermore, we showed that PIC extends the lifespan of C. elegans via the insulin/IGF-1 signaling. However, the effects of PIC on lipid metabolism during fasting state is unknown. Methods We conducted Oil-Red-O assay, Enzyme assay (TG and Free glycerol contents), PCR analysis and lifespan assay. Results In this study, we demonstrated that PIC-treated C. elegans exhibited suppressed lipolysis under fasting as judged by increased lipid accumulation and TG levels with decreased free glycerol level. Consistent with these findings, PIC treatment resulted in decreased mRNA levels of genes involved lipolysis such as atgl-1, hosl-1 and aak-2 in fasted C. elegans. Also, PIC treatment augmented fasting-induced lifespan of C. elegans by an increased daf-16 gene expression. However, such effect was abolished when atgl-1, aak-2, and daf-16 mutants were treated with PIC. In addition, we also found that autophagy is required for PIC-induced lifespan in C. elegans during fasting since autophagy inhibitor treatments and autophagy gene deficient worms resulted in blunting the lifespan extension effect of PIC. Conclusions Collectively, our results indicate that PIC contributes to lifespan extension in C. elegans during fasting possibly through regulating lipolysis- and/or autophagy-dependent lipid metabolism. Funding Sources 1. The National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2019R1A2C1086146) and (2019R1A6A3A03033878) 2. The Rural Development Administration of the Republic of Korea.

Developmental patterns in hormone and lipid metabolism of growing Jinhua and Landrace gilts

2008 ◽  
Vol 88 (4) ◽  
pp. 601-607 ◽  
Author(s):  
Zhi-Guo Miao ◽  
Lei-Jie Wang ◽  
Zi-Rong Xu ◽  
Jin-Feng Huang ◽  
Yan-Rong Wang
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Growth Factor ◽  
Fatty Acid Synthase ◽  
Carcass Characteristics ◽  
Hormone Sensitive Lipase ◽  
Developmental Patterns ◽  
Serum Leptin ◽  
Insulin Levels ◽  
Igf I

The aim of the present study was to investigate the developmental patterns of lipid metabolism, hormones and growth factor in Jinhua and Landrace gilts. Six purebred gilts of each breed were selected and slaughtered for analyses at 35, 80, and 125 d of age, respectively. Jinhua gilts contained less lean meat (P < 0.01), and more carcass fat (P < 0.05) compared with Landrace gilts. Serum leptin, insulin-like growth factor I (IGF-I) and insulin levels increased with age, whereas, free triiodothyronine (FT3) and free thyroxine (FT4) levels decreased with age in both breeds (P < 0.05). Jinhua gilts had lower (P < 0.05) serum IGF-I, FT3 and FT4 levels, and higher (P < 0.05) serum leptin and insulin levels compared with Landrace gilts. Fatty acid synthase (FAS) activity of Jinhua gilts was higher than that of Landrace gilts (P < 0.001), whereas Jinhua gilts had lower hormone-sensitive lipase (HSL) activity (P < 0.001). Jinhua gilts had lower (P < 0.05) levels of free fatty acid (FFA) and triacylglycerol (TG). These results suggest that hormone and growth factors could induce changes in lipid metabolism that inhibit fat synthesis through reducing lipogenic enzymes activities and promote fat degradation by increasing HSL activity, and therefore induce differences in carcass characteristics in the two breeds. Key words: Gilts, lipid metabolism, carcass characteristics, hormone, growth factor

scholarly journals Effects on Liver Lipid Metabolism of the Naturally Occurring Dietary Flavone Luteolin-7-glucoside

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Carla Sá ◽  
Ana Rita Oliveira ◽  
Cátia Machado ◽  
Marisa Azevedo ◽  
Cristina Pereira-Wilson
Keyword(s):  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Molecular Mechanisms ◽  
Metabolic Diseases ◽  
Regulatory Element ◽  
Lipid Lowering ◽  
Whole Body ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Hepatic Expression

Disruptions in whole-body lipid metabolism can lead to the onset of several pathologies such as nonalcoholic fatty liver disease (NAFLD) and cardiovascular diseases (CVDs). The present study aimed at elucidating the molecular mechanisms behind the lipid-lowering effects of the flavone luteolin-7-glucoside (L7G) which we previously showed to improve plasma lipid profile in rats. L7G is abundant in plant foods of Mediterranean diet such as aromatic plants used as herbs. Results show that dietary supplementation with L7G for one week induced the expression of peroxisome proliferator-activated receptor-alpha (PPAR-α) and of its target gene carnitine palmitoyl transferase 1 (CPT-1) in rat liver. L7G showed a tendency to decrease the hepatic expression of sterol regulatory element-binding protein-1 (SREBP-1), without affecting fatty acid synthase (FAS) protein levels. Although SREBP-2 and LDLr mRNA levels did not change, the expression of HMG CoA reductase (HMGCR) was significantly repressed by L7G. L7G also inhibited this enzyme’sin vitroactivity in a dose dependent manner, but only at high and not physiologically relevant concentrations. These results add new evidence that the flavone luteolin-7-glucoside may help in preventing metabolic diseases and clarify the mechanisms underlying the beneficial health effects of diets rich in fruits and vegetables.

Effect of insulin on transcription of lipogenic and lipolytic-related genes and lipid metabolism in primary porcine adipocytes

2006 ◽  
Vol 3 (2) ◽  
pp. 135-140
Author(s):  
Lu Jian-Xiong ◽  
Chen Fen-Fen ◽  
Yang Gong-She
Keyword(s):  
Fatty Acid Synthase ◽  
Lipolytic Activity ◽  
Binding Protein ◽  
Regulatory Element ◽  
Mrna Level ◽  
Hormone Sensitive Lipase ◽  
Mrna Levels ◽  
Tissue Samples ◽  
Element Binding Protein ◽  
High Insulin

AbstractPrimary adipocytes from subcutaneous adipose tissue samples obtained from 7-day-old Yorkshire×Landrace crossbreed piglets were exposed to 0–400 nmol/l of insulin for 48 h. The accumulated triglyceride was measured through Oil Red O staining and the cumulative glycerol released was determined to assess lipolytic activity in adipocytes. Transcription levels of sterol regulatory element binding protein (SREBP)-1c, carbohydrate response element binding protein (ChREBP), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and hormone-sensitive lipase (HSL) were assessed using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that ChREBP and ACC1 mRNA levels were not influenced by insulin alone under low glucose (5 nmol/l). FAS mRNA level was markedly stimulated by all doses of insulin except 200 nmol/l, and SREBP-1c mRNA level increased with 100–300 nmol/l insulin. High insulin doses (300 and 400 nmol/l) increased the HSL mRNA level as well as lipolytic activity.

scholarly journals Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

2020 ◽  
Vol 21 (16) ◽  
pp. 5884
Author(s):  
Sara Balbuena-Pecino ◽  
Esmail Lutfi ◽  
Natàlia Riera-Heredia ◽  
Esther Gasch-Navalón ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
In Vivo Models ◽  
Rainbow Trout Oncorhynchus Mykiss

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

scholarly journals PSXI-27 Association of an increased marbling score by dietary glycerol supplementation with lipid metabolism gene expression in Korean cattle steers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 383-384
Author(s):  
Seon Pil Yoo ◽  
Dilla Fassah ◽  
Myunggi Baik ◽  
sang Weon Na ◽  
Inhyuk Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Mrna Levels ◽  
Lipid Uptake ◽  
Marbling Score ◽  
Adipose Tissues ◽  
Korean Cattle ◽  
Subcutaneous Adipose

Abstract This study investigated effects of dietary glycerol supplementation on liver, muscle, and adipose gene expression related with gluconeogenesis and lipid metabolism and association of gene expression levels with marbling score in Korean cattle steers. Fourteen Korean cattle steers (average age 28.4 months; average body weight 733 kg) were equally assigned to two groups (0 and 5% glycerol supplementation). Glycerol was provided with glycerol (63%)-adsorbed ground wheat bran (37%, DM) by top dressing during roughage feeding. A concentrate (1.2% of body weight) and 1.0 kg of ryegrass were individually fed twice daily. After four months of study, steers were slaughtered, and marbling score was evaluated. Longissimus thoracis (LT) and subcutaneous adipose tissue at the 13th thoracic vertebra area and liver were collected and analyzed for mRNA levels by quantitative real-time PCR. Statistical significance was analyzed by analysis of variance. Correlations were analyzed using Pearson’s correlation analysis. Glycerol supplementation increased (P = 0.01) marbling score. In the LT, glycerol supplementation tended to increase (0.05 &lt; P ≤ 0.10) lipid uptake CD36 and lipoprotein lipase (LPL) mRNA levels. In subcutaneous adipose tissues, glycerol supplementation increased (P ≤ 0.05) LPL, adipogenic sterol regulatory element binding protein 1 (SREBP1), and lipogenic acetyl CoA carboxylase (ACC) mRNA levels and tended to increase (0.05 &lt; P &lt; 0.10) CD36, adipogenic peroxisome proliferator-activated gamma (PPARG), and lipogenic fatty acid synthase (FASN) expression. It did not affect (P &gt; 0.05) mRNA levels of hepatic gluconeogenesis genes. Marbling score showed significant positive correlations (0.57 &lt; r &lt; 0.68; P &lt; 0.05) with mRNA levels of several genes including LPL, PPARG, SREBP1, and ACC in adipose tissues, but not with any genes examined in the LT. Our study demonstrates that lipid uptake, adipogenesis and lipogenesis may mainly contribute to the increased marbling score by glycerol supplementation.

scholarly journals Chronic Microcystin-LR Exposure Induces Abnormal Lipid Metabolism via Endoplasmic Reticulum Stress in Male Zebrafish

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 107 ◽  
Author(s):  
Dandan Zhang ◽  
Wang Lin ◽  
Yinjie Liu ◽  
Honghui Guo ◽  
Lingkai Wang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Lipid Metabolism ◽  
Endoplasmic Reticulum Stress ◽  
Fatty Acid Synthase ◽  
Prolonged Exposure ◽  
Signal Molecules ◽  
Signal Pathways ◽  
Mrna Levels ◽  
Related Factors ◽  
Hepatic Lipid

In order to explore effects of low levels of continuous microcystin-LR (MC-LR) (a cyanotoxin) exposure on hepatic lipid metabolism on the basis of the endoplasmic reticulum stress (ERS) pathway, we exposed adult male zebrafish to MC-LR (0, 1, 5, and 25 μg/L) for 60 days, and hepatic histopathology as well as lipid metabolic parameters were determined with mRNA levels of ERS signal molecules and downstream factors, along with genes associated with lipid metabolism in zebrafish liver. The results revealed that prolonged exposure to MC-LR remarkably altered the levels of hepatic total cholesterol and triglyceride and led to hepatic steatosis, which was also confirmed by hepatic cytoplasmic vacuolization in Hematoxylin/eosin (H&E) stain and lipid droplet accumulation in Oil Red O stain. The severity of hepatic damage and lipidation was increased in a dose-related manner. MC-LR exposure significantly upregulated transcriptional levels of ERS markers including hspa5, mapk8, and chop, indicating the occurrence of ERS in the liver of zebrafish. Concurrently, MC-LR significantly improved mRNA expression of unfolded protein response (UPR) pathway-related genes including atf6, eif2ak3, ern1, and xbp1s, suggesting that all of the three UPR branches were activated by MC-LR. MC-LR also induced significant upregulation of downstream lipid metabolism-related factors and genes including srebf1, srebf2, fatty acid synthase (fasn), acetyl-CoA carboxylase (acaca), stearoyl-CoA desaturase (scd), HMG CoA reductase (hmgcra), and HMG CoA synthase (hmgcs1), and downregulation of genes associated with lipolysis such as triglyceride hydrolase gene (atgl), hormone-sensitive enzyme gene (hsla), and carnitine palmitoyltransferase gene (cpt1aa). Our present results indicated that the cause of hepatic lipid accumulation by MC-LR was mainly by upregulating lipogenic and cholesterol genes but downregulating the expression of lipolytic genes through the induction of srebf1 and srebf2, which were involved in the activation of ERS signal pathways.

scholarly journals 25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathway

2008 ◽  
Vol 295 (6) ◽  
pp. E1369-E1379 ◽  
Author(s):  
Yongjie Ma ◽  
Leyuan Xu ◽  
Daniel Rodriguez-Agudo ◽  
Xiaobo Li ◽  
Douglas M. Heuman ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fatty Acid Synthase ◽  
Lipid Synthesis ◽  
Mrna Levels ◽  
Intracellular Lipids ◽  
Protein Levels ◽  
Regulatory Molecule ◽  
Fas Mrna

The oxysterol receptor LXR is a key transcriptional regulator of lipid metabolism. LXR increases expression of SREBP-1, which in turn regulates at least 32 genes involved in lipid synthesis and transport. We recently identified 25-hydroxycholesterol-3-sulfate (25HC3S) as an important regulatory molecule in the liver. We have now studied the effects of 25HC3S and its precursor, 25-hydroxycholesterol (25HC), on lipid metabolism as mediated by the LXR/SREBP-1 signaling in macrophages. Addition of 25HC3S to human THP-1-derived macrophages markedly decreased nuclear LXR protein levels. 25HC3S administration was followed by dose- and time-dependent decreases in SREBP-1 mature protein and mRNA levels. 25HC3S decreased the expression of SREBP-1-responsive genes, acetyl-CoA carboxylase-1, and fatty acid synthase (FAS) as well as HMGR and LDLR, which are key proteins involved in lipid metabolism. Subsequently, 25HC3S decreased intracellular lipids and increased cell proliferation. In contrast to 25HC3S, 25HC acted as an LXR ligand, increasing ABCA1, ABCG1, SREBP-1, and FAS mRNA levels. In the presence of 25HC3S, 25HC, and LXR agonist T0901317, stimulation of LXR targeting gene expression was repressed. We conclude that 25HC3S acts in macrophages as a cholesterol satiety signal, downregulating cholesterol and fatty acid synthetic pathways via inhibition of LXR/SREBP signaling. A possible role of oxysterol sulfation is proposed.

Fatty acid composition and mRNA expression levels of lipid-metabolic genes in the muscles of ovariectomised young goats

2016 ◽  
Vol 56 (10) ◽  
pp. 1585 ◽  
Author(s):  
Lei Zhang ◽  
Yan-yan Wang ◽  
Zhan-qin Zhou ◽  
Ming-zhe Fu ◽  
Guang Li ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Fatty Acid Synthase ◽  
Biceps Femoris ◽  
Hormone Sensitive Lipase ◽  
Mrna Levels ◽  
Biceps Femoris Muscle ◽  
Metabolic Genes

This study investigated the fatty acid composition and mRNA levels of acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), hormone-sensitive lipase (HSL) and lipoprotein lipase (LPL) genes in the Longissimus dorsi muscle (LD) and Biceps femoris muscle (BF) of nine ovariectomised young goats, 50 days after ovariectomy at 5 months of age, relative to nine Control goats. Ovariectomy resulted in faster growth rates (130 vs 110 g/days; P = 0.027), more intramuscular fat (3.37% vs 1.62%; P = 0.009) and heavier final weights (28.3 vs 26.6 kg; P = 0.035). The total saturated fatty acid, C15:0 in the LD and C6:0, C11:0, C12:0, C15:0 and C21:0 in the BF were lower in ovariectomised goats than that in the Control goats (P < 0.05). Ovariectomy increased the total monounsaturated fatty acids in the LD (P < 0.05) and BF (P < 0.01). In the BF, the total polyunsaturated fatty acids and C18:2 of ovariectomised goats were lower than those of the Control goats (P < 0.05), and the C20:2 content significantly decreased in the LD (P < 0.05). The LD of the ovariectomised goats expressed significantly higher FAS but lower HSL mRNA levels than those of Control goats (P < 0.01). Significantly higher ACC, FAS and LPL and lower HSL mRNA levels were observed in the BF of the ovariectomised goats than in the BF of Controls (P < 0.05). These data suggest that ovariectomy affects fatty acid composition potentially by altering the expression of lipid-metabolic genes in the muscles of young goats.

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