Ingestive patterns of liver-denervated rats presented with several diets

1994 ◽  
Vol 267 (1) ◽  
pp. R44-R52
Author(s):  
L. L. Bellinger ◽  
G. Dula ◽  
F. E. Williams
Keyword(s):  
Food Intake ◽  
High Protein Diet ◽  
Male Rats ◽  
Sham Operation ◽  
Dark Phase ◽  
Sweetened Condensed Milk ◽  
Afferent Nerves ◽  
Condensed Milk ◽  
Body Weights ◽  
Diet Exposure

The liver by way of afferent nerves is suggested to be a controller of food intake. In experiment 1, male rats were given a 15% fructose solution during the first 4 h of the dark phase, while chow was available the rest of the time, for 10 days, before total liver denervation (TLD) or sham operation. Postsurgery ingestion patterns (15-min measurements for 4 h) of fructose were similar in the two groups. However, chow intake in the TLD group was slightly attenuated the first 2 days after surgery. In experiment 2, rats were given chow in cups and vegetable oil in bottles for 8 days before TLD or sham operation. After surgery, hourly ingestion of chow and oil did not differ between the groups; however, there was a trend for the TLD group to take more oil in the dark phase on the first-day diet exposure. In experiment 3, rats were fed a high-protein diet for 21 days before TLD or sham operation. With the use of a computer-operated system, postsurgery meal size, meal duration, and frequency patterns were found to be comparable between the groups. In experiment 4, rats were given a diet of sweetened condensed milk mixed with water (3:1 vol/vol) and vitamins for 14 days before hepatic vagal branch transection (HVBX) or sham operation. After surgery the first-day milk intake of both groups was similar up to 3.5 h and then depressed at 4 and 24 h in the HVBX rats, but was again comparable over the next 13 days; body weights were similar throughout the study.(ABSTRACT TRUNCATED AT 250 WORDS)

Use of orchiectomy and testosterone replacement to explore meal number-to-meal size relationship in male rats

1999 ◽  
Vol 276 (5) ◽  
pp. R1366-R1373 ◽  
Author(s):  
Jia-Ke Chai ◽  
Vladimir Blaha ◽  
Michael M. Meguid ◽  
Alessandro Laviano ◽  
Zhong-Jin Yang ◽  
...  
Keyword(s):  
Food Intake ◽  
Meal Size ◽  
Male Rats ◽  
Testosterone Replacement ◽  
Sham Operation ◽  
Dark Phase ◽  
Light Phase ◽  
Feeding Regulation ◽  
Size Relationship ◽  
Significant Change

Because food intake is a function of meal number and meal size and because gender-related hormones are involved in feeding regulation, we explored effects of orchiectomy and testosterone replacement on the relationship between meal number and size and changes in resulting feeding patterns in adult male rats, randomized into orchiectomy and sham-operation groups. A rat eater meter measured feeding indexes for 1 wk before and 2 wk after castration and during 8 days of testosterone replacement. Orchiectomy leads to an immediate change in the meal number-to-size relationship, resulting in 1) change in pattern of feeding; 2) a significant decrease in dark-phase meal number; 3) a significant increase in dark-phase meal size, but insufficient to offset decrease in meal number, so total food intake significantly decreased during dark phase; 4) no significant change in light-phase meal number; and 5) an increase in meal size leading to an increased food intake during light phase, which offset decreased food intake in dark cycle and resulted in no net significant change in food intake after orchiectomy. Testosterone replacement acutely reversed effects of orchiectomy on meal number-to-meal size relationship, restoring feeding pattern. Data suggest that androgens immediately influence the meal number-to-meal size relationship. The speed of onset seen after orchiectomy suggests that the influence of testosterone on food intake may also occur partially via a nongenomic effect.

scholarly journals Intraperitoneal CCK and Fourth-Intraventricular Apo AIV Require Both Peripheral and NTS CCK1R to Reduce Food Intake in Male Rats

Endocrinology ◽  
2014 ◽  
Vol 155 (5) ◽  
pp. 1700-1707 ◽  
Author(s):  
Chunmin C. Lo ◽  
W. Sean Davidson ◽  
Stephanie K. Hibbard ◽  
Maria Georgievsky ◽  
Alexander Lee ◽  
...  
Keyword(s):  
Food Intake ◽  
High Fat Diet ◽  
Early Gene ◽  
Male Rats ◽  
Reduce Food Intake ◽  
Low Fat Diet ◽  
Afferent Nerves ◽  
Fat Consumption ◽  
Hypothalamic Arcuate Nucleus ◽  
Effective Doses

Apolipoprotein AIV (Apo AIV) and cholecystokinin (CCK) are secreted in response to fat consumption, and both cause satiation via CCK 1 receptor (CCK-1R)-containing vagal afferent nerves to the nucleus of the solitary tract (NTS), where Apo AIV is also synthesized. Fasted male Long-Evans rats received ip CCK-8 or fourth-ventricular (i4vt) Apo AIV alone or in combination. Food intake and c-Fos proteins (a product of the c-Fos immediate-early gene) were assessed. i4vt Apo AIV and/or ip CCK at effective doses reduced food intake and activated c-Fos proteins in the NTS and hypothalamic arcuate nucleus and paraventricular nucleus. Blockade of the CCK-1R by i4vt lorglumide adjacent to the NTS attenuated the satiating and c-Fos-stimulating effects of CCK and Apo AIV, alone or in combination. Maintenance on a high-fat diet (HFD) for 10 weeks resulted in weight gain and attenuation of both the behavioral and c-Fos responses to a greater extent than occurred in low-fat diet-fed and pair-fed HFD animals. These observations suggest that NTS Apo AIV or/and peripheral CCK requires vagal CCK-1R signaling to elicit satiation and that maintenance on a HFD reduces the satiating capacity of these 2 signals.

scholarly journals Brain somatostatin receptor 2 mediates the dipsogenic effect of central somatostatin and cortistatin in rats: role in drinking behavior

2014 ◽  
Vol 307 (7) ◽  
pp. R793-R801 ◽  
Author(s):  
Hiroshi Karasawa ◽  
Seiichi Yakabi ◽  
Lixin Wang ◽  
Andreas Stengel ◽  
Jean Rivier ◽  
...  
Keyword(s):  
Food Intake ◽  
Water Intake ◽  
Somatostatin Receptor ◽  
Drinking Behavior ◽  
Male Rats ◽  
Receptor Subtype ◽  
Dark Phase ◽  
Intracerebroventricular Injection ◽  
Water Ingestion ◽  
Food And Water Intake

Intracerebroventricular injection of stable somatostatin (SST) agonists stimulates food and water intake in rats. We investigated the receptor subtype(s) involved in the dipsogenic effect of intracerebroventricular injection of SST agonists, mechanisms of action, and role. In nonfasted and non-water-deprived male rats with chronic intracerebroventricular cannula, intake of water without food or food without water was monitored separately to avoid any interactions compared with intracerebroventricular vehicle. SST-14 and cortistatin (CST-14) (1 μg/rat icv) increased water intake by 3.1- and 2.7-fold, respectively, while both peptides did not alter food intake at 1 h postinjection in the light phase. By contrast, the stable pan-somatostatin agonist ODT8-SST (1 μg/rat icv) increased both water and food intake by 4.9- and 3.7-fold, respectively. S-346-011, a selective receptor 2 (sst2) agonist (1 μg/rat icv) induced water ingestion, while sst1 or sst4 agonist, injected under the same conditions, did not. The sst2 antagonist S-406-028 (1 μg/rat icv) prevented the 1-h water intake induced by intracerebroventricular ODT8-SST and CST-14. Losartan (100 μg/rat icv), an angiotensin receptor 1 (AT1) antagonist, completely blocked the water consumption induced by intracerebroventricular ODT8-SST, whereas intracerebroventricular injection of S-406-028 did not modify the intracerebroventricular ANG II-induced dipsogenic response. The sst2 antagonist reduced by 40% the increase of the 3-h water intake in the early dark phase. These data indicate that SST-14 and CST-14 interact with sst2 to exert a potent dipsogenic effect, which is mediated downstream by angiotensin-AT1 signaling. These data also indicate that sst2 activation by brain SST-14 and/or CST-14 may play an important role in the regulation of drinking behavior.

scholarly journals High-protein diet selectively reduces fat mass and improves glucose tolerance in Western-type diet-induced obese rats

2013 ◽  
Vol 305 (6) ◽  
pp. R582-R591 ◽  
Author(s):  
Andreas Stengel ◽  
Miriam Goebel-Stengel ◽  
Lixin Wang ◽  
Eugenia Hu ◽  
Hiroshi Karasawa ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Fat Mass ◽  
Peptide Yy ◽  
Selective Reduction ◽  
High Protein Diet ◽  
High Protein ◽  
Male Rats ◽  
Dark Phase ◽  
Obese Rats ◽  
Treatment Of Obesity

Obesity is an increasing health problem. Because drug treatments are limited, diets remain popular. High-protein diets (HPD) reduce body weight (BW), although the mechanisms are unclear. We investigated physiological mechanisms altered by switching diet induced obesity (DIO) rats from Western-type diet (WTD) to HPD. Male rats were fed standard (SD) or WTD (45% calories from fat). After developing DIO (50% of rats), they were switched to SD (15% calories from protein) or HPD (52% calories from protein) for up to 4 weeks. Food intake (FI), BW, body composition, glucose tolerance, insulin sensitivity, and intestinal hormone plasma levels were monitored. Rats fed WTD showed an increased FI and had a 25% greater BW gain after 9 wk compared with SD ( P < 0.05). Diet-induced obese rats switched from WTD to HPD reduced daily FI by 30% on day 1, which lasted to day 9 (−9%) and decreased BW during the 2-wk period compared with SD/SD ( P < 0.05). During these 2 wk, WTD/HPD rats lost 72% more fat mass than WTD/SD ( P < 0.05), whereas lean mass was unaltered. WTD/HPD rats had lower blood glucose than WTD/SD at 30 min postglucose gavage ( P < 0.05). The increase of pancreatic polypeptide and peptide YY during the 2-h dark-phase feeding was higher in WTD/HPD compared with WTD/SD ( P < 0.05). These data indicate that HPD reduces BW in WTD rats, which may be related to decreased FI and the selective reduction of fat mass accompanied by improved glucose tolerance, suggesting relevant benefits of HPD in the treatment of obesity.

scholarly journals Histopathological effects of Averon®, an indigenous herbal formulation, on Cyclophosphamide - induced immunosuppressed male rats

2015 ◽  
Vol 32 (04) ◽  
pp. 236-244
Author(s):  
G. Ukpo ◽  
T. Ehianeta ◽  
A. Adegoke ◽  
O. Salako ◽  
G. Mbaka
Keyword(s):  
Food Intake ◽  
Adverse Effects ◽  
Water Consumption ◽  
Negative Control ◽  
Male Rats ◽  
Therapeutic Monitoring ◽  
Control Group ◽  
Significant Drop ◽  
Herbal Formulation ◽  
Body Weights

Abstract Introduction: The study investigated the effect of an acclaimed immunoboosting herbal formulation, Averon®, which contains Aloe spp on some basal physiological and pathophysiological profiles in immunomodulated male rats. Materials and Methods: Six groups of male rats were used for the study: control group received water and pelletized food ad libitum, Negative control Cyclophosphamide-treated group (30mg/kg i.p), two groups pre-treated with Cyclophosphamide (30mg/kg i.p) and followed by oral 200mg/kg and 400 mg/kg Averon® respectively, two groups orally pre-treated with 200mg/kg and 400 mg/kg Averon® respectively and followed by Cyclophosphamide (30mg/kg i.p) on the last three days. The experimental design was characterized by observations for behavioral changes in the rats, changes in body weight, food consumption, water intake and gross histopathological changes after sacriice. Results: The results revealed the adverse effects in the cyclophosphamide pre-treated groups in the behavioral pattern, significant decrease in body weights at several p-values, significant decrease in food intake (p< 0.05), significant drop in water consumption and toxicological effects on the studied organs. The reverse was true for the Averon®-pretreated groups: significant increase in body weights, food intake and water consumption. The adverse effect of cyclophosphamide was most pronounced on the lungs with little or no amelioration of the adverse effects on the pulmonary milieu. However, there was remarkable recovery on the livers, kidneys and the hearts on treatment with Averon®, particularly at the higher doses. Conclusion: Averon® showed tissue ameliorating potential except on the lungs, hence the need for therapeutic monitoring when administered to immunosuppressed subjects.

Roux-en-Y Gastrointestinal Bypass Promotes Activation of TGR5 and Peptide YY

2020 ◽  
Vol 20 (8) ◽  
pp. 1262-1267
Author(s):  
Haojun Yang ◽  
Hanyang Liu ◽  
YuWen Jiao ◽  
Jun Qian
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Metabolic Diseases ◽  
Peptide Yy ◽  
The Body ◽  
Wild Type ◽  
L Cells ◽  
Body Weights ◽  
Gastrointestinal Bypass ◽  
G Protein Coupled

Background: G protein-coupled bile acid receptor (TGR5) is involved in a number of metabolic diseases. The aim of this study was to identify the role of TGR5 after Roux-en-Y gastric bypass (GBP). Methods: Wild type and TGR5 knockout mice (tgr5-/-) were fed a high-fat diet (HFD) to establish the obesity model. GBP was performed. The changes in body weight and food intake were measured. The levels of TGR5 and peptide YY (PYY) were evaluated by RT-PCR, Western blot, and ELISA. Moreover, the L-cells were separated from wild type and tgr5-/- mice. The levels of PYY in L-cells were evaluated by ELISA. Results: The body weights were significantly decreased after GBP in wild type mice (p<0.05), but not tgr5-/- mice (p>0.05). Food intake was reduced after GBP in wild type mice, but also not significantly affected in tgr5-/- mice (p>0.05). The levels of PYY were significantly increased after GBP compared with the sham group (p<0.05); however, in tgr5-/- mice the expression of PYY was not significantly affected (p>0.05). After INT-777 stimulation in L-cells obtained from murine intestines, the levels of PYY were significantly increased in L-cells tgr5+/+ (p<0.05). Conclusion: Our study suggests that GBP up-regulated the expression of TGR5 in murine intestines, and increased the levels of PYY, which further reduced food intake and decreased the body weight.

Effect of combination of peripheral oxytocin and naltrexone at subthreshold doses on food intake, body weight and feeding-related brain gene expression in male rats

2021 ◽  
pp. 113464
Author(s):  
Mitchell A. Head ◽  
Allen S. Levine ◽  
David G. Christian ◽  
Anica Klockars ◽  
Pawel K. Olszewski
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Food Intake ◽  
Male Rats ◽  
Brain Gene Expression
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