scholarly journals Application of Laser-Induced Bone Therapy by Carbon Dioxide Laser Irradiation in Implant Therapy

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Takahiro Naka ◽  
Satoshi Yokose
Keyword(s):  
Bone Formation ◽  
Laser Irradiation ◽  
Healing Process ◽  
Titanium Implants ◽  
Control Group ◽  
Sham Operation ◽  
Micro Ct ◽  
Rat Tibia ◽  
Implant Therapy ◽  
Laser Group

This study evaluated the application of laser-induced bone therapy (LIBT) to reduce implant healing time in rat tibia. Twenty 10-week-old female Sprague-Dawlay rats were used. The rats received laser irradiation (laser group) or sham operation (control group) on either side of the tibia. Five days after invasion, titanium implants were inserted in proximal tibia. Five, 10, and 20 days after implant placement, tibiae were collected. After taking micro-CT and performing a torque test, the tibiae were decalcified and 8-μm-thick sections were prepared. Specimens were stained with hematoxylin and eosin.Results. Micro-CT images, removal torque values, and histomorphometric analysis data demonstrated a significantly accelerated bone formation in the laser group earlier in the healing process.Conclusion. The use of laser irradiation was effective in promoting bone formation and acquiring osseointegration of titanium implants inserted in rat tibia. LIBT may be suitable for use in implant therapy.

scholarly journals Use of Platelet-Rich Fibrin Associated with Xenograft in Critical Bone Defects: Histomorphometric Study in Rabbits

Symmetry ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1293
Author(s):  
Paulo Wilson Maia ◽  
Marcelo Lucchesi Teixeira ◽  
Luís Guilherme Scavone de Macedo ◽  
Antonio Carlos Aloise ◽  
Celio Amaral Passos Junior ◽  
...  
Keyword(s):  
Bone Formation ◽  
Saline Solution ◽  
Healing Process ◽  
Test Group ◽  
The Other ◽  
Control Group ◽  
Collagen Membrane ◽  
Platelet Rich Fibrin ◽  
Histomorphometric Study ◽  
Significant Difference

Platelet-rich fibrin (PRF) is an autologous material used to improve bone regeneration when associated with bone grafts. It affects tissue angiogenesis, increasing the healing process and, theoretically, presenting potential to increase bone neoformation. The aim of this study was to verify, histomorphometrically, the effects of the association of PRF to a xenograft. Twelve adult white New Zealand rabbits were randomly assigned into two groups containing six animals each. After general anesthesia of the animals, two critical defects of 12 mm were created in the rabbit calvaria, one on each side of the sagittal line. Each defect was filled with the following biomaterials: in the control group (CG), xenograft hydrated with saline solution filling one defect and xenograft hydrated with saline solution covered with collagen membrane on the other side; in the test group (TG), xenograft associated with PRF filling the defect of one side and xenograft associated with PRF covered with collagen membrane on the other side. After eight weeks the animals were euthanized and a histomorphometric analysis was performed. The results showed that in the sites that were covered with collagen membrane, there was no statistically significant difference for all the analyzed parameters. However, when comparing the groups without membrane coverage, a statistically significant difference could be observed for the vital mineralized tissue (VMT) and nonmineralized tissue (NMT) parameters, with more VMT in the test group and more NMT in the control group. Regarding the intragroup comparison, the use of the membrane coverage presented significant outcomes in both groups. Therefore, in this experimental model, PRF did not affect the levels of bone formation when a membrane coverage technique was used. However, higher levels of bone formation were observed in the test group when membrane coverage was not used.

Influence of Homemade and Commercial Fibrin Sealant on Alveolar Bone Healing - an Experimental Study.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4110-4110
Author(s):  
Maria Elvira P. Correa ◽  
Oslei P. Almeida ◽  
Danyel Peres ◽  
Marcelo C. Alves ◽  
Fernando F. Costa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Healing ◽  
Fibrin Sealant ◽  
Factor Xiii ◽  
Alveolar Bone ◽  
Healing Process ◽  
Control Group ◽  
Image Analysis System ◽  
New Bone Formation ◽  
Fibrin Sealants

Abstract The beneficial aspects of fibrin sealants for soft tissues are well documented, but studies of their direct influence on bone healing and their effectiveness in augmenting bone graft healing have produced conflicting results. The aim of this study was to evaluate the influence of fibrin sealants (FS) in the alveolar bone healing process during a period of up to 28 days. Seventy-five Wistar rats were submitted to a superior incisor extraction after intramuscular anesthesia (kentamine chloridrate 10% – 10mg/kg and tiazina chloridrate-5mg/kg). The rats were divided into tree different groups and the sealants were introduced into the alveolar bone. The first group (25) received human homemade fibrin glue, the second group (25) FS associated to factor XIII and aprotinin (Beriplast; Aventis-Beringher) and the third group (25) was the control. Animals were sacrificed by prolonged diethyl inhalation on days 7, 14 and 28 after surgery. Animal craniums were dissected and submitted to a decalcification, and preparated for H&E light microscopy. The morphometric study was performed by means of an interactive computerized image analysis system KS400 (Zeiss, Jena). New bone formation was carefully delimited in four different alveolar regions (apical, two middle areas and cervical) of each specimen. The data were statistically analyzed using multiple regression, ANOVA and Tukey test. Results showed that the amount of alveolar bone formation (μm) in the control group and commercial sealant was statistically similar. However, alveolus receiving homemade sealant presented less amount of new bone formation comparing to commercial sealant and control group (p=0.0034) (figure 1). The present study demonstrated that homemade fibrin sealant delays osteogenic formation. Commercial sealant did not improve alveolar repair however, the amount of new bone formation was slightly higher comparing to the control, probably due to the factor XIII present in this sealant. Figure Figure

scholarly journals Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jiangfeng Liu ◽  
Huijun Kang ◽  
Jiangfeng Lu ◽  
Yike Dai ◽  
Fei Wang
Keyword(s):  
Signaling Pathway ◽  
Control Group ◽  
Osteoporotic Bone ◽  
Sham Operation ◽  
Mrna Levels ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Micro Computed Tomography ◽  
Sham Operation Group ◽  
Pull Out

Abstract Background Poor osseointegration is the key reason for implant failure after arthroplasty,whether under osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine (DFO) can accelerate osteogenesis by activating the hypoxia signaling pathway. The purpose of this study was to test the following hypothesis: after knee replacement, intra-articular injection of DFO will promote osteogenesis and osseointegration with a 3D printed titanium prosthesis in the bones of osteoporotic rats. Materials and methods Ninety female Sprague–Dawley rats were used for the experiment. Ten rats were used to confirm the successful establishment of the osteoporosis model: five rats in the sham operation group and five rats in the ovariectomy group. After ovariectomy and knee arthroplasty were performed, the remaining 80 rats were randomly divided into DFO and control groups (n = 40 per group). The two groups were treated by intraarticular injection of DFO and saline respectively. After 2 weeks, polymerase chain reaction (PCR) and immunohistochemistry were used to evaluate the levels of HIF-1a, VEGF, and CD31. HIF-1a and VEGF have been shown to promote angiogenesis and bone regeneration, and CD31 is an important marker of angiogenesis. After 12 weeks, the specimens were examined by micro-computed tomography (micro-CT), biomechanics, and histopathology to evaluate osteogenesis and osseointegration. Results The results of PCR showed that the mRNA levels of VEGF and CD31 in the DFO group were significantly higher than those in the control group. The immunohistochemistry results indicated that positive cell expression of HIF-1a, VEGF, and CD31 in the DFO group was also higher. Compared with the control group, the micro-CT parameters of BMD, BV/TV, TB. N, and TB. Th were significantly higher. The maximal pull-out force and the bone-to-implant contact value were also higher. Conclusions The local administration of DFO, which is used to activate the HIF-1a signaling pathway, can promote osteogenesis and osseointegration with a prosthesis in osteoporotic bone.

scholarly journals Cigarette smoke inhalation influences bone healing of post-extraction tooth socket: a histometric study in rats

2012 ◽  
Vol 23 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Ana Paula Oliveira Giorgetti ◽  
João Batista César Neto ◽  
Márcio Zaffalon Casati ◽  
Enílson Antonio Sallum ◽  
Francisco Humberto Nociti Júnior
Keyword(s):  
Bone Formation ◽  
Cigarette Smoke ◽  
Bone Healing ◽  
Healing Process ◽  
Test Group ◽  
Smoke Inhalation ◽  
Control Group ◽  
Mineralized Tissue ◽  
Male Wistar Rats ◽  
Socket Healing

The aim of this study was to evaluate, histometrically, the bone healing of the molar extraction socket just after cigarette smoke inhalation (CSI). Forty male Wistar rats were randomly assigned to a test group (animals exposed to CSI, starting 3 days before teeth extraction and maintained until sacrifice; n=20) and a control group (animals never exposed to CSI; n=20). Second mandibular molars were bilaterally extracted and the animals (n=5/group/period) were sacrificed at 3, 7, 10 and 14 days after surgery. Digital images were analyzed according to the following histometric parameters: osteoid tissue (OT), remaining area (RA), mineralized tissue (MT) and non-mineralized tissue (NMT) in the molar socket. Intergroup analysis showed no significant differences at day 3 (p>0.05) for all parameters. On the 7th day, CSI affected negatively (p<0.05) bone formation with respect to NMT and RA (MT: 36%, NMT: 53%, RA: 12%; and MT: 39%, NMT: 29%, RA: 32%, for the control and test groups, respectively). In contrast, no statistically significant differences (p>0.05) were found at days 10 and 14. It may be concluded that CSI may affect socket healing from the early events involved in the healing process, which may be critical for the amount and quality of new-bone formation in smokers.

Bone Formation Around Titanium Implants in the Rat Tibia: Role of Insulin

2003 ◽  
Vol 12 (3) ◽  
pp. 242-251 ◽  
Author(s):  
José T. Siqueira ◽  
Simone C. Cavalher-Machado ◽  
Victor E. Arana-Chavez ◽  
Paulina Sannomiya
Keyword(s):  
Bone Formation ◽  
Titanium Implants ◽  
Rat Tibia

scholarly journals Effects of Low-Intensity Laser Irradiation on Wound Healing in Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Ma ◽  
Ying-xin Li ◽  
Hong-li Chen ◽  
Mei-ling Kang ◽  
Timon Cheng-Yi Liu
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Laser Irradiation ◽  
Healing Process ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Low Intensity ◽  
Intensity Laser ◽  
Later Phase

Objective.The effects of low-intensity 630 nm semiconductor laser irradiation at 3.6 J/cm2(LISL) on wound healing in diabetic rats were studied in this paper.Methods.36 diabetic rats with dorsal cutaneous excisional wounds were divided into three LISL groups and a control group randomly. The three LISL groups were irradiated with LISL at 5, 10, and 20 mW/cm2five times a week for two weeks, respectively. The process of wound healing was assessed by assessing blood glucose, calculating percentage of wound closure, histopathological evaluation, and immunohistochemical quantification.Results.Blood glucose of all groups remained at similar levels throughout the experiment. LISL could obviously promote wound contraction, fibroblasts proliferation, and collagen synthesis, alter bFGF and TGF-β1 expression, and reduce inflammatory reaction in the early and middle phases of chronic wound-healing process. However, LISL could not shorten cicatrization time, and the treatment effects were not sensitive to illuminate parameters in the later phase of the experiment.Conclusions.LISL might have auxiliary effects in the early and middle phases of wound healing in STZ-induced diabetic rats, but the reciprocity rule might not hold. The wound-healing process of early-phase diabetes rats shows typical characteristics of self-limited disease.

scholarly journals Histological and Histomorphometrical Evaluation of a New Implant Macrogeometry. A Sheep Study

2020 ◽  
Vol 17 (10) ◽  
pp. 3477 ◽  
Author(s):  
Sergio Alexandre Gehrke ◽  
Margherita Tumedei ◽  
Jaime Aramburú Júnior ◽  
Tiago Luis Eirles Treichel ◽  
Roni Kolerman ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dental Implants ◽  
Test Group ◽  
Titanium Implants ◽  
Control Group ◽  
New Bone Formation ◽  
Conventional Design ◽  
Random Fashion ◽  
Apical Direction

Decompression or healing chambers between the threads have been proposed to improve and accelerate the osseointegration process of dental implants. The aim of the present work was to test, in an in vivo sheep study, if healing chambers between the threads could produce a better osseointegration process. Thirty titanium implants (15 conventional design (control) and 15 implants with healing chambers (test)) were inserted in a random fashion in the tibia of 3 sheep. The animals were euthanized after 30 days of healing, and the retrieved specimens treated to obtain thin ground sections. Histological observations showed that the quantity of newly formed bone growing in an apical direction was lower in the control group (1095 µm) when compared to the Test group (1658 µm). This difference was statistically significant. Moreover, a layer of osteogenic matrix was present around the portion of implants immersed in the marrow spaces. This osteogenic tissue was thicker in the test group. In conclusion, the present study confirmed the very good results in implants with healing chambers that presented a higher percentage of new bone formation.

scholarly journals Short-term results of the combined application of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and erbium-doped yttrium aluminum garnet (Er:YAG) laser in the treatment of periodontal disease: a randomized controlled trial

2021 ◽  
Author(s):  
Markus Laky ◽  
Maximilian Müller ◽  
Brenda Laky ◽  
Muazzez Arslan ◽  
Christian Wehner ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Yttrium Aluminum Garnet ◽  
Er:Yag Laser ◽  
Periodontal Treatment ◽  
Control Group ◽  
Treatment Results ◽  
Randomized Controlled ◽  
Combined Application ◽  
Yttrium Aluminum ◽  
Laser Group

Abstract Objectives Nd:YAG and Er:YAG lasers have been previously used as an adjunct in periodontal therapy. The aim of this single-blinded randomized controlled clinical trial was to evaluate the efficacy of a combined application of Nd:YAG and Er:YAG laser irradiation in periodontal treatment. Materials and methods Twenty-two patients with at least one site of ≥ 6 mm periodontal probing depth (PPD) after mechanical debridement with curettes and sonic instruments at periodontal reevaluation were included in the study. Patients were randomly allocated at a 1:1 ratio to either a combined Nd:YAG/Er:YAG laser therapy (test group) or a “turned off” laser therapy (control group). The Nd:YAG laser was used for periodontal pocket deepithelialization and to stabilize the resulting blood clot. The Er:YAG laser was primarily used for root surface modification. PPD (mm), clinical attachment level (CAL, mm), and bleeding on probing (BOP, +/−) at the site of laser treatment were evaluated at baseline and 2 months after treatment. Results The mean improvements from baseline to 2-month follow-up for PPD were significantly better in the laser group (2.05 ± 0.82 mm) compared to the control group (0.64 ± 0.90 mm; p = 0.001). Likewise, the gain in CAL was significantly better in the laser group (1.50 ± 1.10 mm) than in the control group (0.55 ± 1.01mm; p = 0.046). Conclusions The combined application of Nd:YAG and Er:YAG laser irradiation as an adjunct to conventional non-surgical therapy showed a significant beneficial effect on periodontal treatment results. Clinical relevance Combined Nd:YAG and Er:YAG laser irradiation could be a useful procedure additionally to conventional non-surgical periodontal therapy to improve periodontal treatment results. Clinical trial registration ISRCTN registry #ISRCTN32132076

scholarly journals Silibinin can enhance the osseointegration of titanium rods in ovariectomized rats maintenance treatment with valproic acid

2021 ◽  
Author(s):  
zhoushan tao
Keyword(s):  
Valproic Acid ◽  
Bone Formation ◽  
Quantitative Polymerase Chain Reaction ◽  
Biomechanical Testing ◽  
Titanium Implants ◽  
Ovariectomized Rats ◽  
Micro Ct ◽  
Alizarin Red ◽  
Combined Application ◽  
Regulated Gene Expression

Abstract The present work was aimed to evaluate the effect of valproic acid(VPA)，Silibinin (SIM)+VPA on Ti rods osseointegration in ovariectomized rats and further investigation of the possible mechanism. The MC3T3-E1 cells were co-cultured with VPA，SIM +VPA and induced to osteogenesis, and the cell viability，mineralization ability were observed by MTT and ALP staining，Alizarin Red staining and Western blotting. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups: group OVX and VPA，SIM +VPA, and all the rats received Ti implants and animals belong to group VPA，SIM +VPA received valproic acid(300 mg/kg/day), valproic acid(300 mg/kg/day) plus SIM (25mg/kg/day) , respectively, treatment until death at 12 weeks. Micro-CT, histology, biomechanical testing, bone metabolism index and Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis were used to observe the therapeutic effect and explore the possible mechanism. Results shown that VPA decreased new bone formation around the surface of titanium rods and push-out force other than group OVX. Histology, Micro-CT and biochemical analysis results showed combined application of systemic VPA showed harmful effects than OVX group on bone formation in osteopenic rats, with the worse effects on CTX-1, P1NP and microarchitecture as well as biomechanical parameters by down-regulated gene expression of Runx2, OCN, Smad1, BMP-2 and OPG, while up-regulated RANKL. However, after SIM treatment, the above indicators were significantly improved. The present study suggests that systemic use of VPA may bring harm to the stability of titanium implants in osteoporosis, SIM can reverse the negative effect of VPA on the osseointegration of titanium rods in ovariectomized rats.

scholarly journals Parathyroid hormone enhances gap healing and osseointegration in orthopedic porous coated titanium implants: a correlative micro-computed tomographic, histomorphometric and biomechanical analysis

2022 ◽  
Vol 23 (1) ◽  
Author(s):  
Xinlin Gao ◽  
Yang Meng ◽  
Dingjun Hao ◽  
Hao Liu
Keyword(s):  
Computed Tomography ◽  
Parathyroid Hormone ◽  
Bone Formation ◽  
Low Dose ◽  
Titanium Implants ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Pull Out ◽  
Custom Made

Abstract Background Parathyroid hormone, with its anabolic effect on bone formation, has shown excellent outcomes of curing postmenopausal osteoporosis as well as enhancing osseointegration around orthopaedic and stomatologic implants.The purpose of the present study is to test if low-dose intermittent PTH (1–34) treatment could achieve a satisfactory osseointegration in 2-mm peri-implant gaps, as to provide a new idea for improving the stability of such prosthesis, which will be of great clinical value. Methods A custom-made titanium implant was implanted on the calvarium of New Zealand White rabbits. 48 male rabbits were randomly divided into control and PTH group. PTH group received subcutaneous injection of PTH (20 μg/day, 5 days/week). Animals were sacrificed at 4 and 8 weeks after surgery. Quantitative micro-computed tomography, histology and biomechanical pull-out testing were performed to evaluate the gap healing at implantation site. Results Analysis of micro-computed tomography demonstrated that PTH group achieved more new bone formation in 2-mm gaps and on bone-implant interface. Quantitatively, significant differences were observed between two groups in regard to BIC and BV/TV at each time-point. Histological staining revealed that PTH group had a superiority in trabecular number, thickness, separation and better osseointegration compared to control group. As for biomechanical pull-out testing, PTH group also showed significant improvement of ultimate force than control group. Conclusions Low-dose intermittent administration of PTH for 4 and 8 weeks enhances early osseointegration and fixation of orthopedic implants surrounded by a 2-mm gap in terms of increased bone regeneration and mechanical stability. These findings suggest PTH a potential for reducing the postoperative complications of implants by improving bone healing at peri-implant gaps.

Sign in / Sign up

Export Citation Format

Share Document