scholarly journals Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Maysa F. Zampa ◽  
Inês Maria de S. Araújo ◽  
José Ribeiro dos Santos Júnior ◽  
Valtencir Zucolotto ◽  
José Roberto de S. A. Leite ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Electrochemical Sensors ◽  
Biological Fluids ◽  
Fundamental Aspect ◽  
Layer By Layer ◽  
Cationic Antimicrobial Peptide ◽  
Nickel Phthalocyanine ◽  
Layer Technique ◽  
Potential Applications ◽  
Pharmaceutical Industries

The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion ofPhyllomedusa hypochondrialisfrogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries.

Evaluation of geometrical benchmark artifacts containing multiple overhang lengths fabricated using material extrusion technique

2020 ◽  
Vol 14 (3) ◽  
pp. 7296-7308
Author(s):  
Siti Nur Humaira Mazlan ◽  
Aini Zuhra Abdul Kadir ◽  
N. H. A. Ngadiman ◽  
M.R. Alkahari
Keyword(s):  
3D Model ◽  
Laser Scanner ◽  
Dimensional Accuracy ◽  
Fused Deposition Modelling ◽  
Layer By Layer ◽  
Fused Deposition ◽  
Layer Technique ◽  
Subtractive Manufacturing ◽  
Manufacturing Features ◽  
Layer Problem

Fused deposition modelling (FDM) is a process of joining materials based on material entrusion technique to produce objects from 3D model using layer-by-layer technique as opposed to subtractive manufacturing. However, many challenges arise in the FDM-printed part such as warping, first layer problem and elephant food that was led to an error in dimensional accuracy of the printed parts especially for the overhanging parts. Hence, in order to investigate the manufacturability of the FDM printed part, various geometrical and manufacturing features were developed using the benchmarking artifacts. Therefore, in this study, new benchmarking artifacts containing multiple overhang lengths were proposed. After the benchmarking artifacts were developed, each of the features were inspected using 3D laser scanner to measure the dimensional accuracy and tolerances. Based on 3D scanned parts, 80% of the fabricated parts were fabricated within ±0.5 mm of dimensional accuracy as compared with the CAD data. In addition, the multiple overhang lengths were also successfully fabricated with a very significant of filament sagging observed.

Highly efficient nanomedicine from cationic antimicrobial peptide-protected Ag nanoclusters

2021 ◽  
Author(s):  
Zhikai Ye ◽  
Haishuang Zhu ◽  
Shan Zhang ◽  
Jing Li ◽  
Jin Wang ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Biological Systems ◽  
Cationic Antimicrobial Peptide ◽  
Highly Efficient ◽  
Antimicrobial Efficiency ◽  
Ag Nanoclusters ◽  
Fine Tune

Designing the homogeneous assembly of the bio–nano interface to fine-tune the interactions between the nanoprobes and biological systems is of prime importance to improve the antimicrobial efficiency of nanomedicines.

Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer-Based Electrochemical Sensors in Biological Fluids

Langmuir ◽  
2021 ◽  
Vol 37 (17) ◽  
pp. 5213-5221
Author(s):  
Alexander Shaver ◽  
Nandini Kundu ◽  
Brian E. Young ◽  
Philip A. Vieira ◽  
Jonathan T. Sczepanski ◽  
...  
Keyword(s):  
Electrochemical Sensors ◽  
Biological Fluids ◽  
Dna Aptamer ◽  
Rapid Signaling

A review of additive manufacturing applications in ophthalmology

2021 ◽  
pp. 095441192110280
Author(s):  
Arivazhagan Pugalendhi ◽  
Rajesh Ranganathan
Keyword(s):  
Additive Manufacturing ◽  
Treatment Planning ◽  
Physical Object ◽  
Layer By Layer ◽  
Case Examples ◽  
Manufacturing Method ◽  
3D Cad ◽  
Stl File ◽  
Potential Applications ◽  
Manufacturing Applications

Additive Manufacturing (AM) capabilities in terms of product customization, manufacture of complex shape, minimal time, and low volume production those are very well suited for medical implants and biological models. AM technology permits the fabrication of physical object based on the 3D CAD model through layer by layer manufacturing method. AM use Magnetic Resonance Image (MRI), Computed Tomography (CT), and 3D scanning images and these data are converted into surface tessellation language (STL) file for fabrication. The applications of AM in ophthalmology includes diagnosis and treatment planning, customized prosthesis, implants, surgical practice/simulation, pre-operative surgical planning, fabrication of assistive tools, surgical tools, and instruments. In this article, development of AM technology in ophthalmology and its potential applications is reviewed. The aim of this study is nurturing an awareness of the engineers and ophthalmologists to enhance the ophthalmic devices and instruments. Here some of the 3D printed case examples of functional prototype and concept prototypes are carried out to understand the capabilities of this technology. This research paper explores the possibility of AM technology that can be successfully executed in the ophthalmology field for developing innovative products. This novel technique is used toward improving the quality of treatment and surgical skills by customization and pre-operative treatment planning which are more promising factors.

scholarly journals Utilization of Mango, Apple and Banana Fruit Peels as Prebiotics and Functional Ingredients

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 584
Author(s):  
Hafza Fasiha Zahid ◽  
Chaminda Senaka Ranadheera ◽  
Zhongxiang Fang ◽  
Said Ajlouni
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Fruit Weight ◽  
Banana Fruit ◽  
Fruit Peel ◽  
Tropical Fruit ◽  
Functional Ingredients ◽  
Potential Applications ◽  
Mango Peel ◽  
Pharmaceutical Industries ◽  
Physical And Chemical

Among the waste by-products generated by the fruit industry (peels, seeds, and skins), fruit peel constitutes the major component. It is estimated that fruit peel accounts for at least 20% of the fresh fruit weight. Fruit peels are considered as major sources of dietary fiber and anticipated to be successfully utilized as prebiotics. This study examined the chemical composition, functional properties and the prebiotic effects of three major tropical fruit peels (apple, banana and mango). The prebiotic effect was tested using three commercial probiotic strains (Lactobacillus rhamnosus, L. casei and Bifidobacterium lactis) individually and in combination. Each probiotic culture was fortified with different concentration (0%, 2% and 4%) of selected fruit peel powder (FPP). Results revealed that all tested FPP significantly (p < 0.05) enhanced the probiotics viable counts, which reached >10 logs after 24 h of incubation. However, the concentration of 2% and 4% FPP showed no significant differences (p > 0.05) on the probiotic viable counts. Additionally, the prebiotic effects of FPP were the same when applied to individual and mixed cultures. This investigation demonstrated that small amount (2%) of apple, banana and mango peel powder could be successfully utilized as prebiotics to enhance the growth of lactic acid bacteria (LAB). Additionally, the studied physical and chemical characteristics of FPP demonstrated their potential applications in the food and pharmaceutical industries as functional ingredients.

scholarly journals Insights into the Action Mechanism of the Antimicrobial Peptide Lasioglossin III

2021 ◽  
Vol 22 (6) ◽  
pp. 2857
Author(s):  
Filomena Battista ◽  
Rosario Oliva ◽  
Pompea Del Vecchio ◽  
Roland Winter ◽  
Luigi Petraccone
Keyword(s):  
Antimicrobial Peptide ◽  
Gram Negative Bacteria ◽  
Lipid Domains ◽  
Action Mechanism ◽  
Cationic Antimicrobial Peptide ◽  
Intracellular Targeting ◽  
Gel Retardation Assay ◽  
Charged Membrane ◽  
Intracellular Target ◽  
Anionic Lipids

Lasioglossin III (LL-III) is a cationic antimicrobial peptide derived from the venom of the eusocial bee Lasioglossum laticeps. LL-III is extremely toxic to both Gram-positive and Gram-negative bacteria, and it exhibits antifungal as well as antitumor activity. Moreover, it shows low hemolytic activity, and it has almost no toxic effects on eukaryotic cells. However, the molecular basis of the LL-III mechanism of action is still unclear. In this study, we characterized by means of calorimetric (DSC) and spectroscopic (CD, fluorescence) techniques its interaction with liposomes composed of a mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-rac-phosphoglycerol (POPG) lipids as a model of the negatively charged membrane of pathogens. For comparison, the interaction of LL-III with the uncharged POPC liposomes was also studied. Our data showed that LL-III preferentially interacted with anionic lipids in the POPC/POPG liposomes and induces the formation of lipid domains. Furthermore, the leakage experiments showed that the peptide could permeabilize the membrane. Interestingly, our DSC results showed that the peptide-membrane interaction occurs in a non-disruptive manner, indicating an intracellular targeting mode of action for this peptide. Consistent with this hypothesis, our gel-retardation assay experiments showed that LL-III could interact with plasmid DNA, suggesting a possible intracellular target.

Pectin-Chitosan Multilayer Coated Microbeads of Diclofenac Sodium Prepared by Layer-by-Layer Technique

2014 ◽  
Vol 1060 ◽  
pp. 45-49
Author(s):  
Kamonrak Cheewatanakornkool ◽  
Pornsak Sriamornsak
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Diclofenac Sodium ◽  
Gastric Fluid ◽  
Layer By Layer ◽  
Release Behavior ◽  
Freeze Dried ◽  
Layer Technique ◽  
Calcium Pectinate ◽  
Layer By Layer Technique

The main objective of this study was to fabricate biopolymer-based microbeads, providing enteric properties and controlled release of diclofenac sodium, using layer-by-layer technique. The calcium pectinate microbeads have been designed and coated with chitosan and pectin multilayers. Drug release was performed in simulate gastric fluid (pH 1.2) for 2 hours, followed by pH 6.8 buffer for 8 hours. The effects of chitosan concentration, number of layer and drying technique on drug release were investigated. The results showed that the calcium pectinate microbeads could be simply prepared by ionotropic gelation and then coated with chitosan and pectin solutions using layer-by-layer procedure. The diameter of the microbeads ranged from 800 to 1000 μm for air-dried samples and from 1 to 2 mm for freeze-dried samples. The freeze-dried microbeads had a rough surface and many pores inside, as observed by SEM. The microbeads coated with 4% chitosan/4% pectin revealed a slower drug release than those coated with 1% chitosan/4% pectin and demonstrated a controlled release pattern. Moreover, different drying techniques and numbers of layer also influenced drug release behavior of the prepared microbeads.

scholarly journals Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

2022 ◽  
Vol 1249 ◽  
pp. 131482
Author(s):  
Mina Răileanu ◽  
Barbara Lonetti ◽  
Charles-Louis Serpentini ◽  
Dominique Goudounèche ◽  
Laure Gibot ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Cationic Antimicrobial Peptide ◽  
Polyion Complex ◽  
Antitumor Properties ◽  
Self Assembled

scholarly journals Layer-by-Layer Assembled Nano-Composite Multilayer Gas Barrier Film Manufactured with Stretchable Substrate

2021 ◽  
Vol 11 (13) ◽  
pp. 5794
Author(s):  
Se-Jung Kim ◽  
Tanyoung Kim ◽  
Dongsoo Kim ◽  
Byeong-Kwon Ju
Keyword(s):  
Transmission Rate ◽  
Significant Loss ◽  
Light Transmittance ◽  
Poly Vinyl Alcohol ◽  
Layer By Layer ◽  
Gas Barrier ◽  
Nano Composite ◽  
Barrier Films ◽  
Potential Applications ◽  
Barrier Film

Most gas barrier films produce cracks that lead to a significant loss of gas barrier integrity when strain is applied. In order to fabricate stretchable gas barrier films with low water permeability and high endurance after stretching, we used polydiallydimethylammonium (PDDA) mixed with graphene oxide (GO) and poly (vinyl alcohol) (PVA) mixed with montmorillonite (MMT). These films were manufactured by layer-by-layer assembly on an Ecoflex/polydimethylsiloxane (PDMS) substrate with pre-strain applied. A total of 30 layers of PDDA (GO)/PVA (MMT) coated on the substrate exhibited a low water vapor transmission rate of 2.5 × 10−2 g/m2 day after 100 cycles of stretching (30% strain). In addition, they exhibited a high light transmittance of 86.54%. Thus, the prepared stretchable gas barrier film has potential applications as a barrier film in transparent and stretchable electronic devices.

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